目的 利用真核系统对E1蛋白进行表达并纯化,建立一种以重组E1蛋白为包被抗原的风疹病毒抗体ELISA检测方法.方法 通过生物信息学预测E1优势抗原表位,根据真核系统表达的特点进行序列优化,全基因合成获得风疹病毒E1优势片段核酸序列,构建真核表达载体,在真核系统内进行诱导表达并纯化,用Western blot证实E1蛋白的抗原性.建立ELISA检测系统,并初步检测100份临床血清标本.结果 SDS-PAGE证实重组E1蛋白在酵母中高表达,Western blot证实该E1蛋白具有良好的抗原性,成功建立了检测IgM间接ELISA方法,对临床血清标本的初步检测显示该试剂盒具有较好的敏感性和特异性.结论 本ELISA检测试剂有较高敏感性和较强的特异性,可进一步开发用于检测风疹病毒的早期感染的试剂盒.

OBJECTIVE:The purpose of the present study was to determine whether type III IFN can modulate the autophagic response in human osteosarcoma cell.METHODS:Human osteosarcoma cell were treated with Interferon-λ1. We investigated that Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells by Matrigel invasion assay. Autophagy were assessed by acridine orange staining, MDC staining and Transmission electron microscopy.RESULTS:In this study, we found that Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells. Acridine orange staining and MDC staining showed that Interferon-λ1 triggered the accumulation of acidic vesicular and autolysosomes in osteosarcoma cell. The acridine orange osteosarcoma cell ratios were 3.6 ± 0.5%, 4.5 ± 0.8%, and 12.4 ± 1.7% after treatment with 1, 10, and 100 ng/mL Interferon-λ1 for 48 h. Osteosarcoma cell cells treated with 100 ng/mL Interferon-λ1 for 48 h developed autophagy some-like characteristics, including single or double-membrane vacuoles containing intact and degraded cellular debris.CONCLUSIONS:Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells. Autophagy can be induced in a dose-dependent manner by treatment with Interferon-λ1 in osteosarcoma cell.

Objective To explore the correlation between series pathogen infection of TORCH and essential hypertension.Methods The blood of 760 hospitalized patients (40-65 years old) were collected, which included 320 cases of essential hypertension specimens and 440 cases of the control samples. Then IgG and IgM antibodies of series pathogens of TORCH in serum were detected by enzyme-linked immunosorbent assay (ELISA). The results of the two groups were compared, respectively.Results The positive rate of human cytomegalovirus (HCMV) IgG antibody in the group of hypertensive patients was higher than that of the control group (χ2= 33.790,P = 0.001), and also herpes simplex virus (HSV) IgG antibody in the group of hypertensive patients was higher than that of the control group (χ2= 7.786,P = 0.003).Conclusion The infection of HCMV, HSV may be important risk factors for essential hypertension.

Autophagy was found to play an antimicrobial or antiparasitic role in the activation of host cells to defend against intracellular pathogens, at the same time, pathogens could compete with host cell and take advantage of autophagy to provide access for its proliferation, but there are few articles for studying the outcome of this competition between host cell and pathogens. Therefore, the aim of our study was to investigate the relationship between autophagy activated by Toxoplasma gondii (T. gondii) and proliferation of T. gondii affected by autophagy in vitro. Firstly, human embryonic fibroblasts (HEF) cells were infected with T. gondii for different times. The monodansylcadaverine (MDC) staining, acridine orange (AO) staining, punctuate GFP-LC3 distribution, and transmission electron microscopy (TEM) assays were conducted, and the results were consistent in showing that gondii infection could induce autophagy. Secondly, HEF cells were infected with T. gondii and treated with autophagy inhibitor bafilomycin A1 or inducer lithium chloride for different times. Giemsa staining was conducted, and the results exhibited that T. gondii infection-induced autophagy could in turn promote T. gondii proliferation. Simultaneously, the results of Giemsa staining also revealed that autophagy inhibitor could reduce the number of each cell infected with T. gondii and inhibit T. gondii proliferation. In contrast, autophagy inducer could increase the number of each cell infected with T. gondii and encourage T. gondii proliferation. Therefore, our study suggests that T. gondii infection could activate autophagy, and this autophagy could in turn facilitate T. gondii proliferation in HEF cells for limiting nutrients.

目的 了解不同月份轮状病毒(RV)在婴幼儿腹泻中的感染情况.方法对226例婴幼儿腹泻患者的粪便,用胶体金法检测其RV抗原.结果 在226例婴幼儿腹泻的粪便标本中,共检测RV阳性78例,阳性率34.5%,不同性别间RV阳性率差异无统计学意义(P>0.05).结论 RV依然是秋季婴幼儿腹泻的主要病原体,10至11月是感染高峰期.

Objective To evaluate the correlation between homocysteine(Hcy),hypersensitivity C-reactive protein(hsCRP),tumor necrosis factor-α(TNF-α)and atherosclerosis(AS)in the maintenance hemodialysis patients(MHD).Methods The level of Hcy,hs-CRP,TNF-α,serum creatinine,serum uric acid,glucose,albumin,triglyceride(TG)and total cholesterol(TC)were measured from 40 patients who had more than 1 years maintenance hemodialysis history.Intima-medial thickness of carotid arteries and the presence of atherosclerotic plaque were measured by ultrasonography.Results In MHD with AS group the level of serum Hcy,hsCRP and TNF-α was significantly higher than that without AS.The difference between the two groups was statistically significance(P<0.05).Meanwhile,there was no significant differences on serum TC,TG,blood pressure,pulse pressure,glucose,albumin and uric acid between two groups.Conclusion The increased level of serum Hcy,hsCRP and TNF-α could be used as independent dangerous factors for AS in MHD patients.

前列腺癌是中老年男性的多发病,在欧美等国居男性癌肿死亡第二位.早期诊断对于降低老龄人群癌症死亡率具有重要意义.作为前列腺癌肿标志的前列腺特异性抗原(PSA),已普遍应用于前列腺癌的早期诊断.我们测定了72例血清样本中PSA浓度,并对1例前列腺癌患者血清中的PSA水平进行动态监测.