Canker is a major fungal disease that causes substantial yield losses in pitaya (Selenicereus monacanthus (Lemaire) D.R.Hunt, syn. Hylocereus polyrhizus (F.A.C. Weber) Britton and Rose; red-fleshed pitaya). However, how fruit ripening and pathogenesis interactively shape fungal communities in fruit peels remains unclear. Here, we investigated the diversity, assembly mechanisms, co-occurrence networks, and functional guilds of fungal communities in healthy and diseased fruit peels at immature (green) and mature (red) stages of ‘Jindu No. 1’ pitaya using ITS1 amplicon sequencing. Our results revealed that fruit maturity exerted stronger effects on fungal community structure than disease status, with ripening reducing diversity and increasing dominance. Notably, disease-induced stage-dependent responses: immature communities shifted from stochastic to deterministic assembly under pathogen selection, whereas mature communities maintained stochastic processes despite infection. Co-occurrence network analysis revealed that healthy mature peels formed highly complex, cooperative networks with dense positive interactions, while healthy immature peels exhibited fragmented, modular structures vulnerable to invasion. Diseased immature peels displayed intermediate network topology, and diseased mature peels showed disrupted connectivity. Functionally, healthy fruits maintained balanced pathotroph-saprotroph-symbiotroph guilds, whereas diseased fruits exhibited higher relative abundance of pathotrophs and saprotrophs, reflecting a shift from symbiotic nutrient cycling toward necrotrophic pathogenicity and decomposition. These findings challenge the single-pathogen paradigm by revealing canker as an ecological process involving community-wide restructuring. They provide a theoretical basis for stage-specific microbiome-targeted disease management in tropical fruits, emphasizing the preservation of stochastic assembly and cooperative network structures to enhance disease resistance.

Bacteria have evolved a large number of two-component signalling systems (TCSs), which are typically composed of a histidine sensor kinase (HK) and a response regulator (RR), to sense environmental changes and modulate subsequent adaptive responses. Here, we describe the involvement of an orphan single-domain RR named EmvR in the virulence, extracellular polysaccharide (EPS) production and cell motilities of the bacterial leaf streak pathogen Xanthomonas oryzae pv. oryzicola (Xoc), which infects rice leaves mainly via stomata and wounds. Deletion of emvR in Xoc reduced virulence when using spraying inoculation but not when using infiltration inoculation. The emvR deletion mutant displayed weakened spreading and enhanced twitching. Additionally, although deletion of emvR did not significantly affect EPS production, overexpression of emvR significantly increased EPS production. Several standard assays revealed that EmvR physically interacts with PilB and represses its ATPase activity. Combining our data with previous findings that PilB provides the energy for type IV pilus (T4P) biogenesis, we conclude that EmvR plays a vital role in modulating Xoc T4P synthesis and in the early stage of Xoc infection through rice stomata. Moreover, our data reveal that EmvR can also interact with the HK of the TCS ColSXOCgx_4036/ColRXOCgx_4037, which positively and negatively affects Xoc spreading and twitching, respectively. We propose a 'one-to-two' TCS working model for the role of ColSXOCgx_4036, ColRXOCgx_4037, and EmvR in modulating Xoc motility.

水稻细菌性条斑病(bacterial leaf streak,BLS)由稻黄单胞菌稻生致病变种(Xanthomonas oryzae pv.oryzicola,Xoc)侵染引起,已成为我国南方水稻种植区的一个重要病害.为了筛选防治BLS的生防菌,以Xoc 野生型菌株GX01 为指示菌,采用含菌平板稀释法和牛津杯法,从花生根际土壤中筛选到一株对Xoc具有拮抗作用的细菌,编号为GX-H6.根据形态学观察、生理生化特征以及 16S rDNA和进化树分析,鉴定该菌株属于沙福芽孢杆菌(Bacillus safensis).拮抗实验表明,B.safensis GX-H6 菌株能够对多种黄单胞菌以及植物病原真菌具有较好的拮抗活性,尤其对引起水稻发生白叶枯病(bacterial blight,BB)的稻黄单胞菌稻致病变种(Xanthomonas oryzae pv.oryzae,Xoo)的拮抗效果最显著.温室和田间水稻植株试验表明,GX-H6 菌株能较好地防治BLS和BB.对GX-H6 菌株的基因组进行分析,发现该菌株的基因组中拥有与抗真菌、环境竞争相关的基因,同时也拥有地衣杆菌素(lichenysin)、植物阿唑霉素(plantazolicin)和溶杆菌素(bacilysin)合成相关基因.这为生产应用提供了新的微生物资源,以及为后续的抑菌机理研究提供了新材料.

[目的]明确火龙果茎溃疡病对火龙果植株茎组织真菌群落的影响,为揭示火龙果溃疡病发生机制及防治技术研究提供理论依据.[方法]采集火龙果健康植株和溃疡病发病植株茎组织,利用Illumina高通量测序平台对植株茎组织真菌rDNA的ITS序列进行测序,分析健康植株与发病植株茎组织中的真菌群落特征差异.[结果]火龙果健康植株茎组织的真菌群落多样性显著高于发病植株(P<0.05,下同),且健康植株与发病植株茎组织的真菌群落组成具有明显差异.与健康植株相比,发病植株茎组织的子囊菌门(Ascomycota)和担子菌门(Basidiomycota)相对丰度较高,分别为49.11%和10.90%,门分类水平的未知真菌(unclassified_k_Fungi)的相对丰度较低(39.97%).在属分类水平上,发病植株茎组织存在8个优势属,其中柱节孢属(Neoscytalidium)的相对丰度最高(33.29%),汉纳酵母属(Hannael-la)、Dirkmeia、丛赤壳科未知属(unclassified_f_Nectriaceae)和刺盘孢属(Colletotrichum)4个优势属的相对丰度显著高于健康植株,未在健康植株检测到柱节孢属、维希尼克氏酵母属(Vishniacozyma)和Meira;健康植株茎组织存在4个优势属,其中地霉属(Geotrichum)和链格孢属(Alternaria)的相对丰度显著高于发病植株.线性判别分析(LEfSe)结果显示,发病植株的柱节孢属和维希尼克氏酵母属以及健康植株的地霉属最能解释健康植株与发病植株间的差异.通过比对FUNGuild数据库预测真菌的营养型,发现发病植株中丰度最高的营养型为病理营养型(15.46%),健康植株中丰度最高的营养型是腐生营养型(7.95%).[结论]火龙果发生茎溃疡病后其茎组织的真菌群落多样性显著下降,真菌群落结构发生改变.当病原营养型真菌显著富集在茎组织中时会破坏茎组织的菌群平衡,可能促进了茎溃疡病的发生.

The pitaya canker disease is a serious fungal disease caused by Neoscytalidium dimidiatum. In recent years, the outbreak of the pitaya canker disease in the world’s pitaya producing areas has seriously affected the pitaya industry development. In order to study the expression level and function of genes responding to stress in N. dimidiatum, it is necessary to screen the reference genes that express stably when the pathogen grows under different cultural conditions. The N. dimidiatum strain LJ02 mycelia cultured in potato dextrose liquid medium（PDW）were taken as the control group, and the mycelia cultured in LB medium, and the mycelia cultured in PDW medium supplemented with hydrogen peroxide, pyrazolyl ester and difenoconazole, respectively, were taken as the treatment groups. The expression stabilities of 18candidate reference genes（CYT1, SDH2＿1, TBCC, SUI1, RPL19, PPH, ATP5B, UBE2＿16, RPL13, UBE2＿2, PRS17, SUCLA2, ATP5A,TUB1＿2, ACT1, EFTU, EF1A and GAPDH）were evaluated by real-time quantitative PCR（RT-qPCR）and stability evaluation software（geNorm, NormFinder, Bestkeeper and RefFinder）. Based on the results of RT-qPCR and software analysis, it was found that the Ct values of 18 candidate genes were within 14.80-24.66 under different culture conditions, with moderate expression levels. The reference gene with the highest stability was CYT1, followed by SUI1, while GAPDH and ACT1 were with the worst expression stability. Using at least two reference genes may improve analysis accuracy of RT-qPCR. At this time, SUCLA2 and ATP5A is the most stable reference gene combination. The results provide a theoretical basis for gene expression analysis in the biological process of N. dimidiatum.

Xrv proteins are a group of regulators in Xanthomonas spp., belonging to the histone-like nucleoid-structuring (H-NS) proteins of Gram-negative bacteria. The rice bacterial leaf streak pathogen Xanthomonas oryzae pv. oryzicola ( Xoc ) harbors three Xrv proteins, the XrvA, XrvB, and XrvC. Here, we report that in Xoc , the XrvB but not XrvA and XrvC is involved in negative regulation of the type III secretion system (T3SS) encoded by hrp genes. As with other Xanthomonas spp., the T3SS is an essential virulence determinant of Xoc and the expression of the hrp genes in Xoc is controlled by the HrpG/HrpX regulatory cascade. HrpG positively regulates the expression of HrpX, which in turn activates the transcription of the hrp genes. We provide evidences to demonstrate that the XrvB binds to the promoter region of hrpG and represses its transcription. Furthermore, we found that XrvB production was induced in the Xoc cells cultured in a nutrient-rich medium compared to a hrp -inducing minimal medium. We also found that in Xoc , the hrpG expression level is inversely correlated with the content of XrvB, and XrvB occupancy at hrpG promoter region is positively correlated with XrvB levels. Our data suggest that XrvB is a determinative factor controlling the expression levels of HrpG. In addition, mutation analysis revealed that the Xoc XrvB also plays positive roles in regulating bacterial growth, cell motility, and stress tolerance. Our findings provide important insights into the molecular mechanism of T3SS expression regulation in Xoc .

The bacterial pathogens Xanthomonas oryzae pathovars oryzae (Xoo) and oryzicola (Xoc) cause leaf blight and leaf streak diseases on rice, respectively. Pathogenesis is largely defined by the virulence genes harboured in the pathogen genome. Recently, we demonstrated that the protein HpaP of the crucifer pathogen Xanthomonas campestris pv. campestris is an enzyme with both ATPase and phosphatase activities, and is involved in regulating the synthesis of virulence factors and the induction of the hypersensitive response (HR). In this study, we investigated the role of HpaP homologues in Xoo and Xoc. We showed that HpaP is required for full virulence of Xoo and Xoc. Deletion of hpaP in Xoo and Xoc led to a reduction in virulence and alteration in the production of virulence factors, including extracellular polysaccharide and cell motility. Comparative transcriptomics and reverse transcription-quantitative PCR assays revealed that in XVM2 medium, a mimic medium of the plant environment, the expression levels of hrp genes (for HR and pathogenicity) were enhanced in the Xoo hpaP deletion mutant compared to the wild type. By contrast, in the same growth conditions, hrp gene expression was decreased in the Xoc hpaP deletion mutant compared to the wild type. However, an opposite expression pattern was observed when the pathogens grew in planta, where the expression of hrp genes was reduced in the Xoo hpaP mutant but increased in the Xoc hpaP mutant. These findings indicate that HpaP plays a divergent role in Xoo and Xoc, which may lead to the different infection strategies employed by these two pathogens.

Signal transduction pathways mediated by sensor histidine kinases and cognate response regulators control a variety of physiological processes in response to environmental conditions in most bacteria. Comparatively little is known about the mechanism(s) by which single-domain response regulators (SD-RRs), which lack a dedicated output domain but harbour a phosphoryl receiver domain, exert their various regulatory effects in bacteria. Here we have examined the role of the SD-RR proteins encoded by the phytopathogen Xanthomonas campestris pv. campestris (Xcc). We describe the identification and characterization of a SD-RR protein named McvR (motility, chemotaxis, and virulence-related response regulator) that is required for virulence and motility regulation in Xcc. Deletion of the mcvR open reading frame caused reduced motility, chemotactic movement, and virulence in Xcc. Global transcriptome analyses revealed the McvR had a broad regulatory role and that most motility and pathogenicity genes were down-regulated in the mcvR mutant. Bacterial two-hybrid and protein pull-down assays revealed that McvR did not physically interact with components of the bacterial flagellum but interacts with other SD-RR proteins (like CheY) and the subset of DNA-binding proteins involved in gene regulation. Site-directed mutagenesis and phosphor-transfer experiments revealed that the aspartyl residue at position 55 of the receiver domain is important for phosphorylation and the regulatory activity of McvR protein. Taken together, the findings describe a previously unrecognized class of SD-RR protein that contributes to the regulation of motility and virulence in Xcc.

通过田间药效试验的方法,评价9种常用杀菌剂对番茄灰霉病的防治效果,根据结果筛选出防治番茄灰霉病的有效药剂.结果 表明,按推荐剂量施药3次,400 g/L嘧霉胺悬浮剂,250 g/L嘧菌酯悬浮剂和50％腐霉利可湿性粉剂的防效分别为86.4％、82.3％和80.5％,高于其他供试药剂的防治效果.因此在生产上推荐轮换使用上述3种防效较高的杀菌剂,在灰霉病发生前期或初期开始喷药,每7～14d施药1次.

[目的]明确广西石漠化地区核桃主要病害种类,为其综合防控提供科学依据.[方法]调查广西河池市凤山县、东兰县、巴马县、天峨县、南丹县、环江县和金城江区等石漠化地区核桃病害的发生情况,并采集病害标本进行病原菌形态学特征观察;采用组织分离法分离病原菌,进行离体或活体接种确定其致病性,结合部分病原菌rDNA-ITS序列测定分析,鉴定主要病害的病原菌.[结果]广西石漠化地区核桃主要病害有胶孢炭疽菌(Colletotrichum gloeosporioides)引起的炭疽病,链格孢菌(Alternaria sp.)引起的黑斑病,胡桃楸拟茎点霉(Phomopsis juglandina)、多隔镰刀菌(Fusarium decemcellulare)和禾谷镰刀菌(F.graminearum)引起的枝枯病,葡萄座腔菌(Botryosphaeria dothidea)引起的溃疡病,腐皮镰刀菌(F.solani)和尖孢镰刀菌(F.oxysporum)引起的根腐病及胡桃球针壳(Phyllactinia juglandis)引起的白粉病.其中,多隔镰刀菌和禾谷镰刀菌引起核桃枝枯病为首次发现.核桃炭疽病可为害核桃的叶片、芽、嫩梢和果实;黑斑病可为害叶片、嫩梢和果实的青皮;枯枝病可为害小枝(尤其是1年生嫩枝);溃疡病可为害核桃树干及主侧枝基部;根腐病可为害根部;白粉病为害叶片.[结论]广西石漠化地区核桃主要病害有6种,其中发生最严重的是炭疽病,其次是黑斑病和枯枝病,生产上需重点防控.其他病害总体上发生较轻,但在局部地区发生严重时也需进行防控.

[目的]明确吡唑醚菌酯与苯醚甲环唑混配对核桃炭疽病菌(Colletotrichum gloeosporioides)的联合毒力和林间防治效果,为核桃炭疽病综合防控提供科学依据.[方法]采用菌丝生长速率法测定吡唑醚菌酯和苯醚甲环唑单剂及其不同配比混剂对核桃炭疽病菌菌丝生长的毒力;利用喷雾法进行林间防治试验,评价吡唑醚菌酯与苯醚甲环唑混剂对核桃炭疽病的林间防治效果.[结果]室内联合毒力测定结果表明,吡唑醚菌酯与苯醚甲环唑按质量比3:2和1:1进行复配对核桃炭疽病菌菌丝生长的毒力表现为增效作用,增效系数分别为1.61和1.57;其他配比的增效系数在0.91～1.41,表现为相加作用.林间对核桃炭疽病的防治试验结果表明,250 g/L吡唑醚菌酯乳油与250 g/L苯醚甲环唑乳油以质量比3:2进行混配(吡唑醚菌酯和苯醚甲环唑的含量分别为150和100 g/L),施用剂量为有效成分125.0、166.7和250.0 mg/L时对核桃炭疽病有很好的防治效果,施药3次后防治效果均在80.0％以上,分别与325 g/L苯甲·嘧菌酯悬浮剂(苯醚甲环唑125 g/L+嘧菌酯200 g/L)施用剂量为有效成分162.5、216.7和325.0 mg/L时的防治效果相当;250 g/L苯醚甲环唑乳油125.0 mg/L的林间防治效果也较好,而250 g/L嘧菌酯悬浮剂166.7 mg/L和250 g/L吡唑醚菌酯乳油166.7 mg/L的防治效果稍低.[结论]吡唑醚菌酯与苯醚甲环唑以质量比3:2混配具有较好的增效作用,林间对核桃炭疽病具有良好的防治效果,可作为防治核桃炭疽病的药剂推广应用.

以“大红”红肉火龙果结果树为观测研究对象,对全年现蕾盛花成熟批次进行命名,通过周年生长动态观测,明确了各批次花和果实的抽生发育时间节点.为方便栽培管理,将结果树的年生长周期划分为相对休眠期、新梢生长期、第一茬花果发育期、第二茬花果发育期、第三茬花果发育期共5个发育阶段;单茬花果发育期又分为“恢复—现蕾—开花—成熟”4个发育小阶段.在试验基础上,结合多年栽培管理经验,集成总结出以“补光延长光照与持续去蕾疏花”为关键调控措施的红肉火龙果一年三茬(熟)栽培周年栽培技术.该技术的应用可缓解不同火龙果产区自然成熟上市日期和产量同步性高,鲜果上市高峰期与市场需求匹配度较低的问题,具有较高的推广应用价值.

The sugarcane smut fungus Sporisorium scitamineum is bipolar and produces sporidia of two different mating types. During infection, haploid cells of opposite mating types can fuse to form dikaryotic hyphae that can colonize plant tissue. Mating and filamentation are therefore essential for S. scitamineum pathogenesis. In this study, we obtained one T-DNA insertion mutant disrupted in the gene encoding the pheromone response factor (Prf1), hereinafter named SsPRF1, of S. scitamineum, via Agrobacterium tumefaciens-mediated transformation (ATMT) mutagenesis. Targeted deletion of SsPRF1 resulted in mutants with phenotypes similar to the T-DNA insertion mutant, including failure to mate with a compatible wild-type partner strain and being non-pathogenic on its host sugarcane. qRT-PCR analyses showed that SsPRF1 was essential for the transcription of pheromone-responsive mating type genes of the a1 locus. These results show that SsPRF1 is involved in mating and pathogenicity and plays a key role in pheromone signaling and filamentous growth in S. scitamineum.

Pitaya fruits collected from Nanning,Qinzhou and Fangchenggang counties were stored at 28 ℃ to investigate the occurrence of postharvest diseases..Pathogens were isolated from the diseased tissue and identified based on morphological characteristics and rDNA-ITS sequence analysis.Results showed that soft rot (Fusarium equiseti (Corda) Sacc.,Gilbertella persicaria (Eddy)Hesselt.),black spot (Alternaria alternata (Fr.) Keissl.,Bipolaris cactivora (Petrak)Alcorn)and canker (Neoscytalidium dimidiatum (Penz.) Crous & Slippers) were detected from the postharvest fruits.Fusarium equiseti causing soft rot disease in dragon fruit was reported at the first time.

[目的]明确吡唑醚菌酯等杀菌剂对火龙果溃疡病的室内抑菌活性和田间防效,为火龙果溃疡病综合防控提供科学依据.[方法]采用菌丝生长速率法测定吡唑醚菌酯等5种杀菌剂对火龙果溃疡病菌菌丝生长的室内抑菌活性;利用田间植株喷雾法评价250 g/L吡唑醚菌酯乳油125.0、166.7和250.0 mg/kg,250 g/L嘧菌酯悬浮剂250.0 mg/kg,430 g/L戊唑醇悬浮剂215.0 mg/kg,10％己唑醇悬浮剂60.0 mg/kg和400 g/L氟硅唑乳油133.3 mg/kg对火龙果溃疡病的田间防治效果.[结果]室内毒力测定结果表明,己唑醇、戊唑醇、氟硅唑和吡唑醚菌酯对火龙果溃疡病菌菌丝生长均有较好的抑制作用,抑制中浓度(EC50)分别为0.0749、0.2325、0.5277和1.8572μg/mL,而嘧菌酯的抑制作用稍弱,EC50为18.5655μg/mL.田间小区防治试验结果证明,250 g/L吡唑醚菌酯乳油对火龙果溃疡病有很好的防效,施药3次后,250 g/L吡唑醚菌酯乳油125.0、166.7和250.0 mg/kg对茎溃疡病的防效分别在60.0％、70.0％和80.0％以上,对果实溃疡病的防效分别在65.0％、75.0％和85.0％以上.250 g/L嘧菌酯悬浮剂250.0 mg/kg和430 g/L戊唑醇悬浮剂215.0 mg/kg对火龙果溃疡病也有较好的防效,分别与250 g/L吡唑醚菌酯乳油166.7和125.0 mg/kg的防效相当.10％己唑醇悬浮剂60.0 mg/kg和400 g/L氟硅唑乳油133.3 mg/kg的田间防效较差.[结论]吡唑醚菌酯、嘧菌酯和戊唑醇对火龙果溃疡病均具有良好的防效,可作为防治火龙果溃疡病的药剂在生产上推广应用.

Sporisorium scitamineum is the causal agent of sugarcane smut, which is one of the most serious constraints to global sugarcane production. S. scitamineum and Ustilago maydis are two closely related smut fungi, that are predicted to harbor similar sexual mating processes/system. To elucidate the molecular basis of sexual mating in S. scitamineum, we identified and deleted the ortholog of mating-specific U. maydis locus b, in S. scitamineum. The resultant b-deletion mutant was defective in mating and pathogenicity in S. scitamineum. Furthermore, a functional b locus heterodimer could trigger filamentous growth without mating in S. scitamineum, and functionally replace the b locus in U. maydis in terms of triggering aerial filament production and forming solopathogenic strains, which do not require sexual mating prior to pathogenicity on the host plants.

[Objective]Genetic diversity of Sporisorium scitamineam from main sugarcane growing area of Guangxi was studied, in order to provide new ideas for comprehensive control of sugarcane smut. [Method]S. scitamineam was collected from main sugarcane growing areas of Guangxi, and genomic DNA were extracted from 70 haploid strains. Optimized random amplified polymorphic DNA(RAPD) reaction system and 10 RAPD primers screened deliberately were used to de-tect polymorphism of S. scitamineam by PCR amplification. Then, according to electrophoretogram, Cluster analysis was conducted to construct phylogenic tree with NTSYS2.1 software. [Result]The results showed that, 10 RAPD primer pair could amplify 4-11 bands each, with a total of 77 bands, each band ranged from 150 to 3000 bp in length, but only 6 polymorphic sites were detected, which accounted for a polymorphic ratio of 7.79% in population. The genetic similarity coefficient of 70 S. scitamineam strains ranged from 0.96 to 1.00, and those strains were clustered into 2 clusters at genet-ic similarity level of 0.96, the first cluster was comprised of 2 strains collected from Xiaoyi town of Hengxian county, while the remaining 68 stains were clustered into the second cluster. [Conclusion]S. scitamineam is at low level of genetic differentiation in Guangxi. Polymorphism of S. scitamineam had no significant correlation with its geographical origin, hosts and mating types.

【Objective】The occurrence of sugarcane yellow leaf virus(SCYLV) disease and geographical distribution of SCYLV genotypes in sugarcane growing areas were investigated to provide scientific references for sugarcane disease-resistant breeding and healthy seedling production of sugarcane. 【Method】Different samples, collected from Guangxi sugarcane areas,were detected by RT-PCR with SCYLV specific primers P1/P2. A distinct fragment for each SCYLV genotype was amplified by RT-PCR using specific primers of BRA-PER-F/ BRA-PER-R,REU-F/ B-REVd and CUB-F/ CUB-R.【Result】The results of SCYLV RT-PCR detection revealed that 630bp genomic fragments were amplified in fifty-seven samples from a total of 124samples. Nucleotide sequence of the RT-PCR products was found to be 95%-99% identical to the sequence of the SCYLV isolates reported in GenBank. The survey of SCYLV results showed that fifty-seven samples were found to be positive by RT-PCR detection from total of 124 samples(infection rate:46.0%). The sugarcane crop was infected by SCYLV most seriously in northern Guangxi as the detection rate was 76.0%. It was found that 50.0% sugarcane cultivars were infected by SCYLV in western Guangxi, 40.0% in mid of Guangxi, 36.8% in southern Guangxi, and 16.6%(the lowest) in eastern Guangxi. The major commercial sugarcane variety of ROC22 was also found to be affected by SCYLV in Liucheng, Yizhou,Qinzhou and Jinguang state farm, which were the major sugarcane areas in Guangxi. The results also showed that the highly susceptible varieties to SCYLV were Liucheng 03-182, GT21, YT55 and YT93-159. The survey of SCYLV genotype results indicated that three virus genotypes including CUB, BRA-PER and REU were identified in Guangxi. Genotypes CUB, BRA-PER, and REU were found63.2%, 56.1%, and 5.3% of the total samples, respectively. Genotype CUB was found in three big sugarcane growing locations(northern, mid and southern Guangxi). Genotype BRA-PER was found located in all five big sugarcane producing locations,whereas genotype REU was found distributed in Liuzhou, Pingxiang and Nanning sugarcane producing areas. 【Conclusion】SCYLV disease has been developing and spreading in sugarcane areas and three genotypes are found distributed in various degrees in sugarcane locations of Guangxi.