γ-aminobutyric acid (GABA) is a four-carbon non-protein amino acid, with many regulatory effects in humans. It aids in regulating blood glucose levels and pressure and is widely recognized for its ability to promote cognitive balance through the alleviation of stress and improvements in sleep quality. The GABA content of longan pulp is higher than that of many other fruits and vegetables; however, much is still unknown about GABA’s biosynthesis in longan. In this study, we found that the GABA content of ‘Baoshi No. 1’ (BS1) pulp was significantly higher than that of ‘Chunxiang’ (CX) pulp. The GAD activity was higher in BS1 pulp than CX pulp, while there was no significant difference in the GABA-T activity. Additionally, five GAD genes were identified in longan, and an analysis of their transcriptional levels showed that only the expression level of DlGAD3 corresponded to the GABA content and GAD activity. DlGAD3 was localized in the cytoplasm, and its transient overexpression promoted an increase in the GABA content in Nicotiana benthamiana leaves. Overall, our results show that DlGAD3 is able to promote the accumulation of GABA and may play a major role in its biosynthesis in longan pulp.

Aroma is a crucial factor influencing the flavor quality and economic value of longan fruits. This study employed a mapping population consisting of 98 F1 progeny of ‘Shixia × Xiangcui’ (exhibiting broad segregation in fruit aroma trait) and their parents. We performed the SNP genotyping through whole-genome resequencing to construct a high-density linkage map, followed by QTL mapping and candidate gene screening for aroma trait. We obtained a total of 554.9 Gbp of sequencing data, with an average depth of approximately 15× for the parents and 12× for the progeny. Three types of SNP markers (lm×ll, nn×np, and hk×hk) were developed, totaling 317 877. After merging with a 100 kb sliding window, 6 134 Bin markers were generated. A first high-density Bin map was constructed, comprising 15 linkage groups with 3 517 Bin markers (containing 264 385 SNPs), covering a total map length of 1 666.79 cM. The average marker interval was 0.48 cM, with 99.18% of gaps being less than 5 cM. Collinearity analysis confirmed the high quality of the map. Fifty-six QTLs were mapped for 9 aroma-related traits, including (E)-2-hexenal, ethyl acetate, ethyl butyrate, ethyl crotonate, (E)-2-hexenoate, ethanol, linalool, ocimene, and total ester content. These QTLs explained 19.8%–51.0% of the phenotypic variation rates and were distributed across 11 linkage groups, with two QTL-rich regions on LG7 and LG8. Seven pleiotropic QTLs were detected. By analyzing the expression patterns of 1 535 annotated genes within the mapped intervals, six candidate genes potentially regulating the synthesis of ester characteristic aroma compounds were identified: aldo-keto reductase AKRs, acetolactate synthase small subunit ALS2, ACC oxidase ACO1-1, and transcription factors bHLH122, AGL103, and bZip1. This work provides novel insights into the fruit aroma formation, and facilitates breeding efforts to improve quality in longan and potentially other fruit crops.

Longan (Dimocarpus longan Lour.) is a commercially valuable tropical fruit crop that contains two antagonistic FLOWERING LOCUS T (FT) homologs involved in regulating flowering time. However, how these FT genes interact with flowering regulators FLOWERING LOCUS D (FD) and APETALA1 (AP1) remains unknown. Four flowering-related genes in longan, DlFT1, DlFT2, DlAP1 and DlFD, were successfully isolated. Expression profiling revealed that all four genes were expressed in leaves and buds across different stages of natural and KClO3-induced floral bud differentiation. Functional characterization through heterologous overexpression in Arabidopsis thaliana showed that DlAP1 significantly promotes early flowering under long-day conditions and induced morphological changes in floral organs and leaves. In contrast, DlFD overexpression had no effect on flowering time. Subcellular localization assays revealed that DlFT1 and DlFT2 localized to both the nucleus and the plasma membrane, while DlAP1 and DlFD localized exclusively to the nucleus. Yeast two-hybrid and bimolecular fluorescence complementation (BiFC) analyses revealed a novel regulatory node: DlFT1 directly interacts with DlAP1, a finding that expands the classical FT-FD-AP1 flowering model. Additionally, DlFD interacts more strongly with DlFT1 than with DlFT2, whereas DlFT1 only interacts with DlAP1, but not DlFT2. These results demonstrate that DlFT1 promotes flowering not only via the conserved FD-dependent pathway but also through direct association with AP1. These findings advance our understanding of the regulatory mechanisms of flowering in longan and provide valuable insights into flowering pathways of perennial woody species.

Loquat (Eriobotrya japonica Lindl.) is a subtropical evergreen tree native to China. Generally, the pigments accumulated in the fruits of cultivated loquats are carotenoids rather than anthocyanins. In this study, we showed that the peel of 'Shanpaisanhao' loquats can accumulate anthocyanins and turn red. We further investigated the mechanisms underlying anthocyanin accumulation in the red-pigmented peels of 'Shanpaisanhao' loquats. RNA-seq analysis demonstrated that anthocyanin accumulation in loquat peel is associated with the upregulation of anthocyanin biosynthetic and transport genes as well as the transcriptional factor EjMYB10. Transient overexpression and dual luciferase assays showed that EjMYB10 could induce weak anthocyanin accumulation in tobacco leaves when co-expressed with PsbHLH3, and activate the promoters of EjF3'H, EjANS and EjUFGT. These results provide information for further elucidating the molecular mechanism of anthocyanin accumulation in the peel of SP3H loquat and for breeding of new red-pigmented loquat cultivars.

Longan is an exotic tropical fruit crop and exhibits off-season flowering induced by potassium chlorate (KClO3), though the molecular mechanisms remain unclear. We assembled a high-quality, 441.5 Mb genome of variety 'Shixia', with a contig N50 at 28.1 Mb, 29, 325 protein-coding genes, 26 telomeres and 15 centromeres. Comparative genomic analysis with lychee revealed structural variations potentially driving gene family expansions related to flavone biosynthesis and disease resistance. Transcriptomic profiling showed that natural flowering appears to be primarily regulated by photoperiod, vernalisation and autonomous pathways, while KClO3-induced flowering may preferentially activate the gibberellin pathway at 5 days after treatment (DAT) and autonomous pathways at 10 DAT, involving putative repression of DlDDF1, DlFLCs and DlSVPs, and up-regulation of DlWRKY75_2. In the perpetual-flowering variety 'Sijimi', elevated expression of photoperiod genes, such as DlCOR28, DlCOR27, DlADO3, DlPRR5, DlGI and DlJMJ30, may explain its perpetual blooming. Overexpression of DlDDF1 in Arabidopsis delayed flowering, partially reversed by KClO3 through an increase in bioactive GA4. Together, KClO3-induced flowering likely involves oxidative stress response and gibberellin signalling via DlDDF1 repression, while natural flowering relies on seasonal cues. These results lay the foundation for longan genetic improvement.

Loquat is an evergreen fruit crop which blooms from autumn–winter, and supports human beings with juicy fruit from late spring to early summer. However, the most traditional cultivars of this crop produce small fruit and bear a much lower yield than its relatives like apple, pear and peach. Large-size cultivars have long been a cherished aim of breeders for improving the production yield of loquat. Agronomic practices like panicle thinning, fruit thinning, growth regulator application, fertilization and so on are easier and more accessible ways for growers to produce large-size loquat fruit on existing production trees. Here, we develop a novel pruning method with an annual double back-cut, which provides vigorous shoot with more leaves and thicker branches for bearing much larger loquat fruit. Cellular observation determined that the vigorous shoot training method motivated cell division to produce larger loquat fruit, and that most of these cell layers were proliferated before the appearance of flower blossoms. Gene expression data of four development stages showed that EjFWL1 and EjFWL2 were notably downregulated in flower buds of the vigorously pruned tree. The data here further confirmed that the cell division capacity during flower development greatly influenced both the flower and fruit size of loquat. More importantly, we developed a novel pruning method to inhibit cell division repressors, promote cell proliferation and enlarge fruit size in loquat.

福圆是立冬本×青壳宝圆的杂交子代,通过选择优良单株,定向培育而成的晚熟优质大果型龙眼新品种.树势中庸,成花易、高坐果率、早丰产;果皮青褐色,果实侧偏圆形,大小均匀,平均单果质量14.6 g;果肉乳白色、半透明,肉质细嫩、较易离核,可溶性固形物含量(w,后同)21.9％,还原糖含量6.5 g·100 g-1,总糖含量17.9 g·100 g-1,维生素C含量90.7 mg·100 g-1,可食率69.4％,味浓甜,品质佳.在福州地区,果实9月中下旬成熟;高接后3年生树平均果穗质量1190.5 g,平均株产7.2 kg;4年生树平均果穗质量1212.6 g,平均株产18.1 kg;丰产、稳产性好,连年结果能力强.适宜在福建福州、四川泸州、重庆万州及相似生态区栽培.

Eriobotrya is an evergreen fruit tree native to South-West China and adjacent countries. There are more than 26 loquat species known in this genus, while E. japonica is the only species yet domesticated to produce fresh fruits from late spring to early summer. Fruits of cultivated loquat are usually orange colored, in contrast to the red color of fruits of wild E. henryi (EH). However, the mechanisms of fruit pigment formation during loquat evolution are yet to be elucidated. To understand these, targeted carotenoid and anthocyanin metabolomics as well as transcriptomics analyses were carried out in this study. The results showed that β-carotene, violaxanthin palmitate and rubixanthin laurate, totally accounted for over 60% of the colored carotenoids, were the major carotenoids in peel of the orange colored 'Jiefangzhong' (JFZ) fruits. Total carotenoids content in JFZ is about 10 times to that of EH, and the expression levels of PSY, ZDS and ZEP in JFZ were 10.69 to 23.26 folds to that in EH at ripen stage. Cyanidin-3-O-galactoside and pelargonidin-3-O-galactoside were the predominant anthocyanins enriched in EH peel. On the contrary, both of them were almost undetectable in JFZ, and the transcript levels of F3H, F3'H, ANS, CHS and CHI in EH were 4.39 to 73.12 folds higher than that in JFZ during fruit pigmentation. In summary, abundant carotenoid deposition in JFZ peel is well correlated with the strong expression of PSY, ZDS and ZEP, while the accumulation of anthocyanin metabolites in EH peel is tightly associated with the notably upregulated expressions of F3H, F3'H, ANS, CHS and CHI. This study was the first to demonstrate the metabolic background of how fruit pigmentations evolved from wild to cultivated loquat species, and provided gene targets for further breeding of more colorful loquat fruits via manipulation of carotenoids and anthocyanin biosynthesis.

Longan (Dimocarpus longan Lour.) is an important economic crop widely planted in tropical and subtropical regions, and flower and fruit development play decisive effects on the longan yield and fruit quality formation. MCM1, AGAMOUS, DEFICIENS, Serum Response Factor (MADS)-box transcription factor family plays important roles for the flowering time, floral organ identity, and fruit development in plants. However, there is no systematic information of MADS-box family in longan. In this study, 114 MADS-box genes were identified from the longan genome, phylogenetic analysis divided them into type I (Mα, Mβ, Mγ) and type II (MIKC*, MIKCC) groups, and MIKCC genes were further clustered into 12 subfamilies. Comparative genomic analysis of 12 representative plant species revealed the conservation of type II in Sapindaceae and analysis of cis-elements revealed that Dof transcription factors might directly regulate the MIKCC genes. An ABCDE model was proposed for longan based on the phylogenetic analysis and expression patterns of MADS-box genes. Transcriptome analysis revealed that MIKCC genes showed wide expression spectrums, particularly in reproductive organs. From 35 days after KClO3 treatment, 11 MIKC genes were up-regulated, suggesting a crucial role in off-season flower induction, while DlFLC, DlSOC1, DlSVP, and DlSVP-LIKE may act as the inhibitors. The gene expression patterns of longan fruit development indicated that DlSTK, DlSEP1/2, and DlMADS53 could be involved in fruit growth and ripening. This paper carried out the whole genome identification and analysis of the longan MADS-box family for the first time, which provides new insights for further understanding its function in flowers and fruit.

Huataifeng (HTF) is an eminent cultivar selected from the crossing of Shixia and Xiangcui created and evaluated at National Longan Germplasm Nursery in Fuzhou, Fujian, China. Shixia is a prominent cultivar with high quality and fertility native to Guangdong, populous among Chinese cus-tomers. Xiangcui is a bud-mutation from Biew Khiew (a Thailand cultivar) with large fruit size and abundant fragrance. The hybrid was created in 2009 via artificial pollination, 148 seeds were gathered and consequently 112 seedlings were obtained. Line 09-1-70 (ultimately named HTF) was selected from the cross population for its early bearing capacity (fruiting initiated in 2012). Longan production suffered severe yield lose in Fujian province in 2013 due to off-year bearing, while HTF had a high flowering rate and yield as usual. The stable flower formation of this line might give rise to stable fruit production. It was then to top-grafted onto a mature longan tree in 2013. The grafted trees started to bear fruit in 2014, and from 2014 to 2017 all the grafted trees showed stable and consistent characteris-tic including high flesh recovery, high total soluble sugar content and abundant fragrance. Its fruit weight is 27.8%-48.2% higher than that of Shixia. From 2018 to 2021, HTF was the most consistent bearing line and showed no alternative bearing problem. Consequently, in 2021, HTF was certified in Fujian Province (no. 9352021Y0075). This cultivar has a strong growth vigor and a semi-open tree ar-chitecture. The bark is gray-brown and split into narrow rough ridges. Twigs are red-brown and smooth.The mature compound leaves are in size of (14.1-26.0) cm × (12.2-25.1) cm, and their 4-5 pairs of leaf-lets are green, lanceolate, and (12.8-18.7) cm × (3.9-4.7) cm in size. The leaf margins are wavy, and apex narrowly acuminate. The juvenile phase of 3 years of this cultivar is rather short. And it forms in-florescence every year. Its flower cluster is large and scattered. The oblate fruit are uniform in size with a blue-brown pericarp. The fruit base is flat, sometimes with one risen shoulder and the fruit tip is round. The skin segments are obvious. The fruit has an average longitudinal diameter of 2.73-2.81 cm, average transverse diameter of 2.89-2.93 cm, and average lateral diameter of 2.67-2.70 cm. The aril is milky, translucent, fragrant, tender and crisp, and can be easily separated from the seed. Average fruit weight is (12.7±1.0) g. The flesh recovery is (70.8±1.6)%, and TSS (22.7±2.8)%. HTF has an excellent grafting affinity to many main cultivars, such as Shixia and Chuliang (mainly cultivated in Guangdong province, Hainan province and Guangxi Autonomous Region), Fuyan, Songfengben, Lidongben (main-ly cultivated in Fujian province), and Shuguan, Lufeng (mainly cultivated in Sichuan province). The productivity of 7-year-old grafted trees can be up to 26.3 tons per ha (density 600). Bud breaking of HTF begins in late February, similar to that of Shixia, and the first leaf matured in late May. Inflores-cence morphological differentiation initiates in late February; flowers bloom from late April to early May, and ends in late May. The fruit matures during early September to early October in Fuzhou.

利用SLAF-seq测序技术,开发一批特异性强、稳定性高的单核苷酸多态性(SNP)位点,为枇杷遗传图谱构建、分子辅助育种和物种进化等研究提供理论依据.本研究共收集294份枇杷属资源,利用SLAF-seq测序技术进行测序.以梨基因组为参考基因组进行电子酶切预测,确定使用HaeⅢ+Hpy166Ⅱ进行酶切,构建SLAF-seq文库,而后筛选314～364 bp长度的DNA片段定义为SLAF标签,预测可得到116 171个SLAF标签.共获得526.63 Mreads数据,各样品所获得的读长数目在119893～9305 152范围内,平均读长为1 787581;测序质量值Q30的范围在88.26％～95.67％,平均为93.61％;GC含量分布在38.79％～42.92％范围内,平均值为40.35％.本实验以水稻测序获得0.16 M reads的数据量为Control,双端比对效率在91.28％,说明SLAF建库基本正常.生物信息学分析结果显示本研究共获得623 356个SLAF标签,且有123 498个多态性的标签,多态性为19.81％.在多态性SLAF标签上开发得到1 604 434个群体SNP标记.根据完整度＞0.8,MAF＞0.05过滤,共得到95 960个高一致性的群体SNP.

[目的]Aux/IAA作为重要的生长素原初响应因子参与到植物的各个生长发育阶段.为了解Aux/IAA家族基因在龙眼花芽分化过程中的作用,对DlIAA基因家族进行全基因组鉴定和分析,并研究其在外源激素处理下的表达模式.[方法]利用生物信息学方法对Aux/IAA基因家族进行全基因组鉴定与分析,基于RNA-seq数据库分析DlIAA基因家族的表达,利用qRT-PCR探究DlIAA基因家族在龙眼花芽分化过程中和外源IAA处理下的表达情况.[结果]利用龙眼参考基因组数据,鉴定出18个DlIAA基因,依据龙眼、苹果、拟南芥和番茄之间的系统发育关系,可将进化树分为4大类7个亚族.结果 表明,DlIAA基因家族与苹果具有较高的同源性,而与拟南芥、番茄的亲缘关系较远.基因结构和结构域中同一类群的不同DlIAA基因家族成员具有较高的相似度.启动子中顺式元件的分布表明,DlIAA基因表达不仅受生长素(AuxREs、TGA-element和TGA-box)的调节,还受其他激素和生长环境的调节.DlIAA基因家族在龙眼9个不同组织中表现出不同的表达模式,且部分DlIAA基因为组织特异性基因.通过对不同品种龙眼花芽的3个RNA-seq数据库筛选发现,有5个候选基因(D1IAA2、D1IAA4、D1IAA7、D1IAA15和D1IAA 17)均显示出较低的表达量,表明其功能与龙眼花芽分化可能存在一定的关系.进一步的qRT-PCR分析表明,DlIAA2和DlIAA17可能在开花诱导中起作用.DlIAA对外源IAA的反应结果表明,5个候选基因在100 μmol.L-1 IAA处理下被不同程度地诱导表达,且在500 μmol.L-1浓度下均显著上调.[结论]转录组数据显示DlIAA基因参与了龙眼花芽分化过程,DlIAA2和DlIAA17可能在开花诱导中起作用.

Rambutan is a popular tropical fruit known for its exotic appearance, has long flexible spines on shells, extraordinary aril growth, desirable nutrition, and a favorable taste. The genome of an elite rambutan cultivar Baoyan 7 was assembled into 328 Mb in 16 pseudo-chromosomes. Comparative genomics analysis between rambutan and lychee revealed that rambutan chromosomes 8 and 12 are collinear with lychee chromosome 1, which resulted in a chromosome fission event in rambutan (n = 16) or a fusion event in lychee (n = 15) after their divergence from a common ancestor 15.7 million years ago. Root development genes played a crucial role in spine development, such as endoplasmic reticulum pathway genes, jasmonic acid response genes, vascular bundle development genes, and K+ transport genes. Aril development was regulated by D-class genes (STK and SHP1), plant hormone and phenylpropanoid biosynthesis genes, and sugar metabolism genes. The lower rate of male sterility of hermaphroditic flowers appears to be regulated by MYB24. Population genomic analyses revealed genes in selective sweeps during domestication that are related to fruit morphology and environment stress response. These findings enhance our understanding of spine and aril development and provide genomic resources for rambutan improvement.

Amino acids are important component of fruit nutrition and quality. In this study, three longan cultivars, including non-aroma types ‘Shixia’ (SX), ‘Lidongben’ (LDB), and strong aroma type ‘Xiangcui’ (XC), were selected to analyze free amino acids (FAAs) variations at six distinct growth stages (S1–S6). The genome-wide identification and expression analysis of genes related to the branched-chain amino acids (BCAA) synthesis pathway were carried out. Results showed that 36 FAAs were identified, and the total FAAs content ranged from 2601.0 to 9073.5 mg/kg, which increased drastically with fruit development until ripening. L-glutamic acid (Glu), L-alanine (Ala), L-arginine (Arg), γ-Aminobutyric acid (GABA), L-aspartic acid (Asp), L-leucine (Leu), hydroxyl-proline (Hypro), and L-serine (Ser) were the predominant FAAs (1619.9–7213.9 mg/kg) in pulp, accounting for 62.28–92.05% of the total amino acids. During the period of rapid fruit expansion (S2–S4), the aroma of XC changed from light to strong, and the contents of L-alanine (Ala) and L-leucine (Leu) were significantly higher than those of SX and LDB. Furthermore, a total of two 2-isopropyl malate synthase (IPMS), two 3-isopropyl malate dehydrogenase (IPMD), and 16 BCAA transferase (BCAT) genes were identified. The expression levels of DilBCAT1, -6, and -9 genes in XC were significantly higher than those in SX and LDB, while DilBCAT16 in XC was lower. The content of Leu was negatively correlated with the expression of DilBCAT1, -6, and -9 in three varieties, but positively correlated with DilBCAT16, indicating that these four genes may be responsible for the different synthesis and degradation of Leu among cultivars.

Loquat (Eriobotrya japonica) is a subtropical tree that blooms in autumn, and its fruits ripen during late spring. Sunburn caused by high temperature and high light intensity is one of the most severe abiotic stress factors that decreases loquat productivity and fruit quality and shelf life, greatly inhibiting sustainable development of this fruit crop industry. To date, little is known about fruit heat tolerance regulation, and investigation of heat stress responses and thermotolerant improvement is important for fruit crops. 'Wuduiyadanben' loquat fruits under 40 degrees C stress were collected for physiological and comparative sequencing analyses; hormone changes in loquat fruit in response to heat stress were investigated using liquid chromatography-mass spectrometry (LC-MS) analysis. Several discriminatory hormone metabolites were identified, including fourteen cytokinins (CK), eight auxins, five jasmonic acids (JA), three gibberellin acids (GA), two abscisic acids (ABA), two salicylic acids (SA), one ethylene (ETH) and one strigolactone (SL). Transcriptomics analysis suggested that genes enriched in 'Plant hormone signal transduction', 'Protein processing in endoplasmic reticulum', 'Photosynthesis', 'Plant-pathogen interaction' and 'Oxidative phosphorylation' pathways are involved in the heat stress response. Additionally, small RNA analysis further revealed that miRNAs, including miRNA160-x, miRNA393-x, miR6187-x, miR8726-y and novel-m0057-5p, might act together with auxin signaling and heat shock proteins to regulate genes in the heat stress response process. In summary, this study provides a comprehensive understanding of the complex molecular mechanisms underlying the thermal stress responses of loquat fruit.

本试验利用氨基酸自动分析仪,建立龙眼果实游离氨基酸组分的准确测定方法,并对晚熟品种'立冬本'氨基酸组成及含量进行分析.结果表明,氨基酸混合标准溶液中38种组分在色谱图中得到了较好分离.'立冬本'龙眼果实上共检测到30种游离氨基酸,总含量达5 942.7 mg kg-1,蛋白和非蛋白游离氨基酸占比分别为83.00％和17.00％;必需氨基酸(6种)总含量为245.1 mg kg-1,占比4.12％;鲜味氨基酸和甜味氨基酸含量丰富,占比分别为52.83％和25.08％,苦味氨基酸含量相对较少;与香气物质合成相关的氨基酸(6种)含量占比为21.85％.

为探究枇杷(Eriobotrya japonica)杂交品种真实性和DNA指纹图谱,对新育成的19个枇杷杂交品种(系)进行SSR标记鉴定分析.结果 表明,从已发表的89对SSR引物中筛选出扩增条带清晰稳定的多态性引物19对,在24份枇杷材料中共扩增到83条带,每对引物平均扩增4.37条,PIC值为0.234～0.983,平均为0.764.经12对具有父本特征带多态性引物鉴定,19个杂交新品种(系)全部为真杂种,真杂种率为100％.UPGMA聚类分析表明,19个杂交新品种(系)的遗传相似系数为0.728～0.969,与杂交亲本‘新白2号’和‘贵妃’聚为同一个大类,并可细分为4个亚类,红肉与白肉的枇杷品种(系)间无明显划分.同时利用8对多态性SSR引物组合,构建了19个枇杷杂交新品种(系)的分子指纹图谱.这为枇杷品种鉴定、新品种权保护和杂交育种提供重要参考依据.

一、“十四五”种业发展面临的机遇与挑战 (一)物种产业形势分析 荔枝龙眼是原产于我国南方的名贵南亚热带水果,无论是品种资源还是栽培面积与产量均居世界之首.我国拥有丰富的荔枝龙眼种质资源,但随着全球经济一体化进程的不断加快和生物技术的迅猛发展,国际间的种业竞争也越来越激烈,呈现出从种质资源主权保护到基因资源产权保护的发展态势.因此,加快推进荔枝龙眼种业发展与科技创新,培育和推广具有自主知识产权的优良品种,加强新品种权保护的立法与监察管理,已成为继续保持我国荔枝龙眼种业国际领先地位、促进我国荔枝龙眼产业可持续发展的迫切需要.

失眠日益成为影响健康的重要限制因子,本研究探讨了龙眼冻干粉对失眠小鼠的体重影响,以期为龙眼功能化研究提供参考.结果显示,腹腔注射氯苯丙胺酸(PCPA)后试验组小鼠体重出现持续下降,模型组试验小鼠的体重在第21 d比第19 d减少6.47％(p<0.05),表明PCPA腹腔注射后模型构建成功.试验小鼠摄入龙眼鲜果冻干粉水提取物后可减少PCPA对体重发育的影响,第20 d小鼠体重降低0.51％,仅为模型组的13.93％;第21 d试验小鼠体重下降大幅减少;到第22 d体重就开始增加,有效缓解了PCPA模型对试验小鼠体重发育的影响,表明龙眼具有改善失眠的应用潜力.