The steel frame structure with fuse system is realized by integrating a linked-column frame structure with an energy dissipation system. ABAQUS software was used in this study to assess the structural response of this type of structure by modeling the fuse system. This model was then used to examine how the reduced beam section (RBS) geometry impacts seismic performance. The effects of story height, fuse system span, RBS connection beam strength, and fuse system layout on the structure’s overall performance were examined through pushover analysis. Assuming small deformation and using the principle of virtual work, formulas were established to calculate the elastic lateral stiffness and ultimate bearing capacity of the steel frame with fuse system. The study recommends specific values for the distance from the end plate to the RBS, the extent of flange reduction, and the depth of the flange cut as 0.65bf, 0.65hb, and 0.2bf, respectively. Adjusting the story height and fuse beam span has a negligible impact on the displacement angle range in the structure’s rapid repair stage. RBS connection beams made of lower-grade steel than the steel frames cause the fuse system to yield earlier, and placing the fuse system at the side span is more effective than positioning it at the center. The proposed method for calculating lateral stiffness and ultimate load capacity shows an accuracy within a 10% margin of error compared to finite element analysis results.

To solve the problem of decentralized energy dissipation in the eccentric frame structure, a new energy dissipation substructure called a fuse system is proposed. Based on this, the non-linear finite element analysis on a fuse system is conducted to investigate the failure mode and seismic behavior. Four series of parametric analyses are performed, in which four main influential factors are considered, including length of reduced beams, distance from the reduced starting point to the end plate, reduced length, and reduced depth. The results of the analysis indicate that decreasing the reduced distance and length, increasing the length of reduced beams, and selecting moderately reduced depth can improve the performance of the whole structure. Finally, design commendations based on the analysis are presented.

In order to make up for the shortage of lateral stiffness of traditional lateral force resisting systems and reduce the seismic requirements of steel frames for joints, we proposed a new lateral force resisting system called Viscoelastic Damping Horizontal Corrugated steel shear wall System (VDHCS), which was considered by sliding long hole viscoelastic dampers, corrugated steel plates, and peripheral steel frames. The nonlinear finite element model of the new lateral force-resisting system was established by ABAQUS finite element software. The lateral force resisting capacity of the system was analyzed by displacement loading method to study the influence of viscoelastic damper, thickness, and wavelength of corrugated steel plate on the bearing capacity. The results showed that the system which added a viscoelastic damper possesses better lateral resistance. With the increase of plate thickness and wavelength, the amplification of each lateral resistance index of the system shows a downward trend. Compared with the wavelength of corrugated steel plate, the thickness has a greater impact on the bearing capacity of the system.

A new prefabricated special-shaped steel lattice column structure was proposed to solve the contradiction between the convex column and the insufficient lateral stiffness in the traditional prefabricated steel structure. Based on the double-coefficient product method, a calculation method for the bearing capacity of special-shaped steel lattice columns was provided, considering the influence of eccentricity. Parameter analysis was conducted using ABAQUS based on model verification. The influence mechanism of key factors such as the converted slenderness ratio and eccentricity on the bearing capacity of special-shaped steel lattice columns was studied. The load-displacement curve was obtained, revealing the failure modes of special-shaped steel lattice columns under different stress states. The results showed that the ultimate bearing capacity of special-shaped steel lattice columns is positively correlated with the number of column limbs; the influence of column height on the flexibility of L-shaped steel lattice columns is more obvious; the greater the eccentricity, the ultimate bearing capacity of special-shaped steel lattice column decreases more sharply.

Energy dissipation has been studied as a subject light in the fields of construction. This study is an effort to solve the problems of high energy consumption and low thermal comfort of traditional rural residences in the Shandong province of China. A typical rural residence was selected and tested for analyzing and evaluating the thermal behavior and energy consumption of the building envelope. The results indicate that the tested residence has ultra-high energy consumption and low thermal comfort because of poor thermal performance and excessive shape coefficient. An energy-saving modification for exiting residences is proposed through theoretical analysis and design. Implementation of a modification scheme to rural residences could meet the energy saving standard of 65% and decrease the energy consumption of traditional rural residences by 70%.

In order to solve the problems of wet operation, low assembly rate, and insufficient integrity of prefabricated floor slab, a total-prefabricated steel structure composite slab was proposed. First, ABAQUS was used to verify the finite element modeling method, based on six finite element models established to study the ultimate bearing capacity and failure modes of three special corrugated plates under different boundary conditions. The results showed that the ultimate bearing capacity of the three plate types under simply supported conditions is 1.12–1.47 times the design load; the ultimate bearing capacity under the condition of fixed support is 4.37–5.48 times the design load. The floor has sufficient safety reserve to meet the load-bearing design requirements, making it safe and reliable.

BackgroundNF-κB is one of the most important inflammatory mediators in the tumour microenvironment promotes inflammation-induced cancer. Many studies report that NF-κB is activated in many kinds of leukaemia, so that some researchers by inhibiting NF-κB to treat leukaemia. The overexpression of BCL11B has been primarily reported in T cell malignancies. Some studies have reported that BCL11B is a potential transcriptional repressor which has several splice isoforms. MiR-21-5p promotes cell proliferation by targeting BCL11B in Thp-1 human monocytes. Both NF-κB and BCL11B transcription factors are related to inflammation and leukaemia. Whether there is correlation between NF-κB and BCL11B transcription factors? If BCL11B is a potential important transcription factor in treatment of T lymphocyte leukaemia? In this study, we stimulated Jurkat cells and normal peripheral blood T cells with staphylococcal enterotoxin A(SEA), and we detected the expression of both the BCL11B and NF-κB genes and their respective proteins at different stimulation times. Then, the BCL11B gene was suppressed by BCL11B-siRNA and detected at another stimulation time. ResultsWe found that the mRNA expression of BCL11B and NF-κB increased in two kinds of T cell lines over time which stimulated by SEA or PMA+IO. A similar result was confirmed for BCL11B and NF-κB protein expression. While the expression of the NF-κB protein did not increase on equal conditions in the BCL11B-knockdown group. ConclusionOur result suggested that the gene and protein expression levels of both BCL11B and NF-κB were related, and BCL11B regulates NF-κB expression in Jukat cells and healthy human peripheral blood through the TCR signalling pathway. This study reveals that BCL11B can be used as a new therapeutic target for chronic inflammation and T cell leukaemia pathogenesis.

目的:探讨中药重楼(Paris polyphylla,PP)活性单体PP-11体外抑制人乳腺癌MDA-MB-231细胞增殖的作用及其机制.方法:采用不同浓度的PP-11作用于MDA-MB-231细胞,采用MTT法和集落形成实验检测细胞增殖情况;Hoechst 33258染色及Annexin V-FITC/PI双染流式细胞术检测细胞凋亡;JC-1染色检测线粒体膜电位改变;Western blot法检测细胞凋亡及自噬相关蛋白表达情况.再采用总caspase抑制剂Z-VAD-FMK、p38抑制剂SB203580和自噬抑制剂氯喹(chloroquine,CQ)进行阻断实验.结果:MTT测定结果显示,PP-11以剂量和时间依赖方式显著抑制MDA-MB-231细胞活力,其作用24、48和72 h后的IC50分别为5.64、4.58和3.06μmol/L.集落形成实验结果提示,PP-11显著抑制MDA-MB-231细胞集落形成.Hoechst 33258染色观察到PP-11组MDA-MB-231细胞出现典型的细胞凋亡形态.Annexin V-FITC/PI双染流式细胞术结果显示,随着PP-11浓度的增加,MDA-MB-231细胞凋亡率逐渐升高.JC-1染色结果显示,PP-11处理的MDA-MB-231细胞线粒体膜电位下降.Western blot检测结果显示,PP-11处理的MDA-MB-231细胞Bcl-2家族蛋白中抗凋亡蛋白Bcl-2和Bcl-xL的表达显著减少,促凋亡蛋白Bim和Bok表达增加,p-p38和p-p53蛋白水平升高,p-ERK蛋白水平降低,cleaved caspase-9、cleaved cas?pase-3及cleaved PARP的蛋白水平显著升高;PP-11降低MDA-MB-231细胞p-STAT3蛋白水平及其下游蛋白c-Myc、cyclin D和Mcl-1的表达.总caspase抑制剂Z-VAD-FMK和p38 MAPK抑制剂SB203580均可减弱PP-11诱导的细胞凋亡.随着PP-11作用浓度的增加,细胞自噬相关蛋白LC3-Ⅱ表达增加,p62/SQSTM1表达下降;采用PP-11联合自噬阻断剂CQ,可逆转PP-11诱导的cleaved PARP表达,并提高细胞活力(P<0.05).结论:重楼单体PP-11可显著抑制人乳腺癌MDA-MB-231细胞增殖.PP-11通过激活p38 MAPK信号通路、抑制ERK和JAK-Stat3信号通路诱导MDA-MB-231细胞发生线粒体相关的凋亡,并促进细胞自噬.

Abstract Aberrant T cell activation is a major cause of aplastic anemia (AA) pathogenesis. Recent studies have shown that miRNAs regulate T cell activation and are involved in AA. A previous study found that miR-214 was significantly up-regulated upon T cell activation in a CD28-dependent fashion by targeting PTEN. However, the expression characteristics of miR-214 and its target genes in AA have not been defined. In this study, target genes for miR-214 were predicted and confirmed by bioinformatics and luciferase reporter assays. The expression levels of miR-214 and target genes were detected in 36 healthy individuals and 35 patients with AA in peripheral blood mononuclear cells by real-time quantitative reverse transcriptase-polymerase chain reaction. Bioinformatics and luciferase reporter assays identified that miR-214 could bind to the A20 3′ untranslated regions. Significantly increased miR-214 and the decreased A20 expression level were detected in the AA patients compared with the healthy group. In addition, significantly increased miR-214 was found in non-severe aplastic anemia compared with severe aplastic anemia patients. These results suggested that the A20 gene was a potential target of miR-214, and elevated miR-214 might medicate T cell activation at least in part by regulating A20 expression in AA. We firstly confirmed that miR-214 regulated A20 expression, and aberrant miR-214/A20 expression might contribute to immunopathology in AA. The miR-214 expression might be used as a potential biomarker that assisted in diagnosing AA severity.

As a buckling-resistant component, the corrugated steel shear wall was adopted to restrain the local buckling of the steel plate. The mild steel damper was also added to improve the energy dissipation capacity of the structure, forming a new damping energy-dissipation corrugated steel plate shear wall system (DCSW). In order to study the hysteresis behaviour of DCSW, 5 nonlinear FEMs were established by ABAQUS, with the consideration of corrugated steel plate placement, mild steel dampers arrangement and openings form. Their ultimate bearing capacity, ductility, stiffness and energy dissipation performance were compared and evaluated. The results indicated that the DCSW system had better bearing capacity and energy dissipation capacity. The ultimate bearing capacity of structure with corrugated steel plate shear walls and mild steel dampers increased by 37%~43% and 11.4%~13% respectively, combining using those two elements, their ultimate bearing capacity increased by 52%~56%, and their ductility was also improved significantly.

In order to study the seismic behavior of corrugated steel shear wall, a three dimensional nonlinear FEM of steel corrugated shear wall was established by use of ABQUS software. Based on that, the bearing capacity of the corrugated steel shear wall under the monotonic loads and its hysteretic behavior under the cyclic loads were analyzed. The results indicate that the corrugated steel shear wall could decrease storey drift and improve lateral bearing capacity and stiffness of steel frames dramatically; the corrugated steel shear wall has better seismic performance than steel plate shear wall.

The central brace combined with a ring damper is used as lateral resistant to improve the lateral stiffness and seismic performance of steel frame. In order to reveal the working mechanism of the new system, three nonlinear finite element models: steel frame with central brace, steel frame with eccentric brace, and steel frame infill central brace with steel ring damper, were established. The results indicate that compared with other two structural systems, the steel frame infill central brace with steel ring damper has the best bearing capacity, energy dissipation ability and seismic behaviour.

In order to improve the lateral stiffness of the steel frame, natural anti-buckling corrugated steel shear wall was combined with mild steel dampers, forming a new damping energy-dissipation corrugated steel plate shear wall system (DCSW). The design method of the DCSW system was proposed based on plastic analysis. To study the seismic performance of this system, the nonlinear FEM of the DCSW system was established by ABAQUS, and the analysis on bearing capacity and energy dissipation capacity was carried out. The results showed that the DCSW designed by this method had high lateral bearing capacity and energy dissipation capacity. The lateral ultimate bearing capacity increased by 50% and lateral displacement reduced by 43% with corrugated steel plate shear wall and mild steel dampers.

In order to avoid the premature buckling of steel plate shear walls, the corrugated steel plate walls are used to increase stiffness and the buckling resistance performance, and soft steel dampers are also adopted to improve the energy dissipation capacity. This combined use of the corrugated steel plate wall and the damper leads to a new system: damping energy-dissipating corrugated steel shear wall (DCSW). A numerical simulation model was established to study the seismic behavior of DCSW using the software ABAQUS, in which the material and geometric nonlinear were considered. Based on that, the ultimate bearing capacity, stiffness and ductility were analyzed. The results indicate that the DCSW structure has a high strength redundancy. The strength of DCSW structure is about 18% higher than that of steel.

1 ABIN概述 ABIN蛋白家族包含ABIN1、ABIN2、ABIN3三种蛋白,这三种蛋白都显示出有限的序列同源性:含有同源区域AHD1和泛素交联区域UBAN(图1). AHD1可介导ABIN蛋白与A20相结合,UBAN可介导ABIN与泛素相交联,通过ABIN结合泛素、A20编辑泛素的方式来抑制NF-κB的活化,这体现出了ABIN蛋白家族的同一性. 在组织分布和抑制激活上,ABIN蛋白家族表现出明显的差异性.ABIN1大量表达在活化的免疫组织和细胞上,与NF-κB亚型中的p105和p100相互作用,p105和p100具有抑制转录的作用,ABIN1通过A20编辑泛素或竞争泛素链来激活P105和P100,最终抑制NF-κB活化.

Objective:To investigate mechanism of imatinib mesylate induced apoptosis of primary CD3+T cells.Methods:The CD3+T cells were stimulated by 0-100 nmol/L imatinib for 24 h,cell apoptosis was detected by flow cytometry;Caspase-3,Caspase-8, A20 and NF-κB expression levels were detected by Real-time quantitative PCR and Western blot.Results: IM significantly increased apoptosis of T cell;Caspase-3 and A20 gene expression levels were upregulated and NF-κB expression level was downregulated both in gene and protein levels.Conclusion:IM increased apoptosis of T cell by upregulating A20 expression.

Th17 cells,as a subset of CD4 + helper T cell population,are characterized by secreting inflammatory cytokines IL-17 and participating in the inflammatory response.Th17 cells are not only found to play inflammatory effect,but also play a important role in the occurrence and development of tumor as well as in antitumor response.Studies have found that Thl7 cells and related cytokines exist in a variety of tumors tissue.Th17 cells could enhance and regulate anti-tumor immune response through secreting IL-17 to affect relevant antitumor signaling pathway.In addition,the innate immune cells of IL-17 + gamma delta T cells and IL-17 + Foxp3T cells not only secrete IL-17 to play a role in the stimulation of tumor,but also rate with Th17 cells to regulate the anti-tumor immune response.This reriew intends to focns on the mechanism of Th17 involving the tumor response,including interaction with the IL-17 + gamma delta T cells and IL-17 + Foxp3T cells.

This study aimed to investigate the effects of harmine hydrochloride (HMH) on digestive tumor cells in vitro and its molecular mechanism. MTT assays showed that HMH inhibited the proliferation of some human cancer cell lines and had no obvious inhibitory effects on human LO2 cells. Flow cytometry assays showed that HMH trigged G2 phase arrest in MGC‐803 cells and SMMC‐7721 cells, while the expression of cyclin A, cyclin B, p21, Myt1, and p‐cdc2 (Tyr15) was upregulated. Flow cytometry assays also showed that the percentages of apoptotic cells were increased, the mitochondrial transmembrane potential (ΔΨm) decreased, and the cleavage of caspase‐9, caspase‐3, and poly (Adenosine diphosphate ribose) polymerase (PARP) were observed, the expression of Bad increased, phospho‐Bad (S112) decreased, pro‐caspase‐8 was cleaved, and Bid (22 kDa) was cleaved. The expression of p‐ERK decreased in both cells. In conclusion, these results demonstrated that HMH upregulates the expression of p21, activates Myt1 and inhibits cdc2 by phospho‐cdc2 (Y15), and triggers G2 phase arrest in both MGC‐803 cells and SMMC‐7721 cells. It can also activate the mitochondria‐related cell apoptosis pathway through the caspase‐8/Bid pathway, inhibiting the ERK/Bad pathway and promoting apoptosis in both of these two cell types. Copyright © 2015 John Wiley & Sons, Ltd.