PurposeThe purpose of this study is to evaluate the 12-year outcomes of bedside laser photocoagulation (LP) for severe retinopathy of prematurity (ROP) under sedation combined with ocular surface anesthesia in neonatal intensive care units (NICU).DesignThe study is a retrospective case series.MethodsInfants treated with bedside LP for severe ROP from April 2009 to September 2021 were included. All LP treatments were performed under sedation and surface anesthesia at the bedside in NICU. Data were recorded for clinical and demographic characteristics, total laser spots, duration of treatment, proportion of total regression of ROP, proportion of recurrence, and adverse events.ResultsA total of 364 infants (715 eyes) were included, with a mean gestational age of 28.6 & PLUSMN; 2.4 weeks (range: 22.6-36.6 weeks) and a mean birth weight of 1,156.0 & PLUSMN; 339.0 g (range: 480-2,200 g). The mean number of laser spots was 832 & PLUSMN; 469, and the mean duration of treatment was 23.5 & PLUSMN; 5.3 min per eye. Of all the eyes, 98.3% responded to LP with complete regression of ROP. ROP recurred in 15 (2.1%) eyes after the initial LP. Additional LP was performed in seven (1.0%) eyes. No patient exhibited mistaken LP of other ocular tissues, and there were no serious ocular adverse effects. None of them needed endotracheal intubation.ConclusionsBedside LP treatment is effective and safe for premature infants with severe ROP under sedation and surface anesthesia in NICU, especially for infants whose general condition is unstable and not suitable for transport.

[目的]本研究旨在评估循环胆红素谱[总胆红素(TBIL)、结合胆红素(DBIL)、未结合胆红素(IBIL)]与非增殖期糖尿病视网膜病变(NPDR)的相关性.[方法]采用病例对照研究设计,通过人工智能眼底相机对2型糖尿病患者视网膜病变情况进行筛查,并由眼科医生进一步阅片确认病例.最终纳入312名2型糖尿病(T2DM)患者(78例新诊断的NPDR患者与234例无糖尿病视网膜病变的T2DM患者进行1:3匹配).同时收集患者人口学和相关临床资料,检测患者血清胆红素及相关生化指标.[结果]NPDR患者的TBIL、DBIL和IBIL浓度显著降低(P值分别为0.003、0.001、0.006),且与糖化血红蛋白(HbA1c)水平无关(P值均>0.05).在调整了性别、糖尿病病程、HbA1c和收缩压等传统风险因素后,这种关联仍然存在.此外,在血糖控制良好的T2DM患者(HbA1c≤7％)或无微量白蛋白尿的T2DM患者[尿白蛋白/肌酐比值(UACR)≤30 mg/g]中,低水平未结合胆红素者的NPDR风险更高,比值比(OR)95％CI分别为3.44(1.04,11.38)和2.53(1.10,5.82);在血糖控制不良(HbA1c>7％)或有微量白蛋白尿(UACR>30 mg/g)的T2DM患者中,低水平结合胆红素者的NPDR风险更高,OR 95％CI分别为2.05(1.09,3.86)和2.40(1.14,5.02).[结论]循环胆红素与NPDR的风险呈独立的负相关关系,这可能成为早期预测和防治糖尿病视网膜病变的临床生物标志物.

PURPOSE: To report three-decade changes of clinical characteristics, progress of treatments, and risk factors associated with mortality and enucleation in patients with retinoblastoma in China. DESIGN: Retrospective cohort study. METHODS: This multicenter study included 2552 patients diagnosed with retinoblastoma in 38 medical centers in 31 provinces in China from 1989 to 2017, with follow-up data. Kendall's tau-b value was used to describe correlation coefficients between the three eras (between 1989 and 2008, between 2009 and 2013, and between 2014 and 2017) and clinical or demographic features. Hazard ratios and odds ratios were applied to measure risk factors. RESULTS: A total of 324 (13%) patients died and 1414 (42%) eyes were removed. The 1-year, 3-year, and 5-year overall survival rates were 95%, 86%, and 83%, respectively. Patients were diagnosed at a better stage by International Classification for Retinoblastoma over time (Kendall's tau-b value = -0.084, P < .001). Pathological risk factors were also observed less in recent eras. New conservative therapies were adopted and used in more patients. The eye removal rate gradually decreased (Kendall's tau-b value = -0.167, P < .001). The over all survival rates were 81%, 83%, and 91% in the three eras. By multivariate Cox regression, bilateral tumors and extraocular extension were identified as risk factors for death. Among intraocular disease, Group E indicated higher risk of mortality. By multivariate logistics regression, unilateral tumors, earlier era of diagnosis, and extraocular extension were risk factors for eye salvage failure. Among intraocular retinoblastoma, Groups D and E had higher risk of eye salvage failure. CONCLUSIONS: Patients were diagnosed at an earlier stage in recent eras. Conservative therapies, including intra-arterial chemotherapy, were increasingly being used. The above changes may contribute to the decreasing enucleation rate. Although no significant impact was identified on the mortality by the three eras, a decreasing trend was shown. (C) 2021 Elsevier Inc. All rights reserved.

Purpose: This study attempted to estimate the impact of eye-preserving therapies for the long-term prognosis of patients with advanced retinoblastoma with regard to overall survival and ocular salvage. Design: Retrospective cohort study covering all 31 provinces (38 retinoblastoma treating centers) of mainland China. Participants: One thousand six hundred seventy-eight patients diagnosed with group D or E retinoblastoma from January 2006 through May 2016. Methods: Chart review was performed. The patients were divided into primary enucleation and eye preserving groups, and they were followed up for survival status. The impact of initial treatment on survival was evaluated by Cox analyses. Main Outcome Measures: Overall survival and final eye preservation. Results: After a median follow-up of 43.9 months, 196 patients (12%) died, and the 5-year overall survival was 86%. In total, the eyeball preservation rate was 48%. In this cohort, 1172 patients (70%) had unilateral retinoblastoma, whereas 506 patients (30%) had bilateral disease. For patients with unilateral disease, 570 eyes (49%) underwent primary enucleation, and 602 patients (51%) received eye-preserving therapies initially. During the follow-up (median, 45.6 months), 59 patients (10%) from the primary enucleation group and 56 patients (9.3%) from the eye-preserving group died. Multivariate Cox analyses indicated no significant difference in overall survival between the 2 groups (hazard ratio [HR], 1.25; 95% confidence interval [CI], 0.85-1.84; P = 0.250). For patients with bilateral disease, 95 eyes (19%) underwent primary enucleation, and 411 patients (81%) received eye-preserving therapies initially. During the follow-up (median, 40.1 months), 12 patients (13%) from the primary enucleation group and 69 patients (17%) from the eye-preserving group died. For bilateral retinoblastoma with the worse eye classified as group E, patients undergoing primary enucleation exhibited better overall survival (HR, 2.35; 95% CI, 1.10-5.01; P = 0.027); however, this survival advantage was not evident until passing 22.6 months after initial diagnosis. Conclusions: Eye-preserving therapies have been used widely for advanced retinoblastoma in China. Patients with bilateral disease whose worse eye was classified as group E and who initially underwent eye preserving therapies exhibited a worse overall survival. The choice of primary treatment for advanced retinoblastoma should be weighed carefully. Ophthalmology 2022;129:209-219 (c) 2021 by the American Academy of Ophthalmology

目的:采用角膜激光共焦显微镜观察研究颗粒状角膜营养不良家系患者的角膜状况.方法:采用角膜激光共焦显微镜对4例(6只眼)颗粒状角膜营养不良患者的角膜进行扫描检测,对比裂隙灯及普通显微镜观察分析检查情况.结果:颗粒状角膜营养不良患者的病变角膜裂隙灯下呈现面包屑样的灰白色混浊,在苏木精-伊红(hematoxylin-eosin,HE)染色中呈现出伊红染的颗粒,沉积于基质板层之间,在共焦显微镜下则为中高反光的团块,相互融合,并取代了基质细胞.结论:颗粒状角膜营养不良患者角膜病变从裂隙灯到普通HE染色下的显微镜,乃至高倍的共焦显微镜观察到的沉积物,分布情况相吻合,印证角膜病变发展的状况,有利于了解该病变的发展情况.

目的 探讨玻璃体腔注射康柏西普治疗眼底血管性疾病的临床疗效及安全性.方法 选取30例(34只眼)眼底血管性疾病患者作为研究对象,均给予玻璃体腔注射康柏西普治疗.治疗后3~12个月随访,采用最佳矫正视力表检测治疗前后最佳矫正视力(BCVA)值,使用同一设备行光学相干断层扫描(OCT)检查,观察患者黄斑中心凹厚度(CTM)值,详细记录治疗期间患者的不良反应情况.结果 30例患者共实施玻璃体腔注射康柏西普88次,每只眼注射次数1~6次,平均注射次数(3.4±10.5)次,其中注射≥2次26只眼.注射后有2例2只眼发生结膜下出血,但未见积血等并发症,未经处理后自行消失;另有部分患者自述注射后眼部有短暂异物感,24 h后逐渐消失且并无其他不适感.治疗后,中心性浆液性脉络膜视网膜病变、中心性渗出性脉络膜视网膜病变患者的BCVA显著高于治疗前,差异有统计学意义(P<0.05),湿性年龄相关性黄斑变性(wAMD)、糖尿病视网膜病变(DR)、视网膜静脉阻塞(RVO)患者的BCVA与治疗前比较,差异无统计学意义(P>0.05),但是患者的视物模糊、视物变形等主观感受均有显著改善.治疗后所有患者的CTM值均较治疗前有明显改善,差异有统计学意义(P<0.05).结论 玻璃体腔注射康柏西普治疗眼底血管性疾病,能有效改善患者临床症状,疗效显著,安全性高,值得临床推广应用.

While Plastic Compressed (PC) collagen technique is often used to fabricate bioengineered constructs, PC collagen gels are too weak to be sutured or conveniently handled for clinical applications. To overcome this limitation, electrospun poly (lactic-co-glycolide) (PLGA) mats, which have excellent biocompatibility and mechanical properties, were combined with PC collagen to fabricate sandwich-like hybrid constructs. By laser-perforating holes with different sizes and spacings in the electrospun mats to regulate the mechanical properties and light transmittance of the hybrid constructs, we produced hybrid constructs with properties very suitable to apply in corneal tissue engineering. The maximum tensile stress of the optimal hybrid construct was 3.42 ± 0.22 MPa. The light transmittance of the hybrid construct after perforation was approximately 15-fold higher than before, and light transmittance increased gradually with increasing time. After immersing into PBS for 7 days, the transmittance of the optimal construct changed from 63 ± 2.17% to 72 ± 1.8% under 500 nm wavelength. The live/dead staining, cell proliferation assay and immunohistochemistry study of human corneal epithelial cells (HCECs) and human keratocytes (HKs) cultured on the optimal hybrid construct both demonstrated that the cells adhered, proliferated, and maintained their phenotype well on the material. In addition, after culturing for 2 weeks, the HCECs could form stratified layers. Thus, our designed construct is suitable for the construction of engineered corneal tissue.

Purpose: To compare the efficacy of intravitreal injection of ranibizumab (IVR) monotherapy and laser therapy for treatment-requiring retinopathy of prematurity (ROP) in Zone II.Methods: A prospective, randomized, controlled single-center trial was applied from January 2014 to December 2014; infants who were diagnosed as Zone II treatmentrequiring ROP (i.e., Zone II Stage 2 or 3 ROP with plus disease) were randomly assigned to receive IVR monotherapy or laser therapy, and the follow-up interval was at least 6 months.Any eyes that developed recurrence of ROP underwent crossover re-treatment.Results: A total of 100 eyes of 50 ethnic Han Chinese infants were enrolled.At the last follow-up, 26 eyes of 13 infants developed recurrence of ROP in the IVR group and 2 eyes of 1 infant developed recurrence of ROP in the laser therapy group.There was a significant statistical difference in the rate of ROP recurrence between IVR and laser therapy to treat Zone II treatment-requiring ROP (P = 0.001).Conclusion: Although IVR appears to regress ROP to certain levels and continue to promote the vascularization of peripheral retinal vessels, a substantial proportion of infants developed recurrence of ROP after a single-dose IVR.Therefore, IVR is not recommended as a single-dose monotherapy for Zone II treatment-requiring ROP.

Objective: To characterize the comprehensive treatment of retinoblastoma (RB) and investigate the related clinical factors. Methods: In this retrospective study, clinical datas were analyzed for 65 cases (92 eyes) of childhood RB that received comprehensive treatment, including chemotherapy, local treatment (laser or cryotherapy), and enucleation. Using t-test and Fisher's exact test to analyze the data. Results: The survival rate for the 65 cases was 92%. The eyeballs were preserved in 51% of the children. The rate of eyeball preservation in stage E group was significantly lower than the other four stage groups (P<0.01). Children in stage D group (18 cases) and stage E group (24 cases) were treated with chemotherapy combined with local therapy. The eyeballs were preserved in 11 cases in stage D groups and 4 cases in stage E groups. There were no significant differences in the clinical factors between eyeballs preserved or not children.The patients in stage D and E groups were further divided into three groups according to the location of the tumors in the eye: it showed peripheral retina (including the mid- peripheral retina) group with higher rate of eyeball preservation than the posterior pole retina and the posterior pole retina+peripheral retina group (P=0.009, 0.021). Four of the 42 patients died, all with tumors located in the posterior pole retina (P=0.045 compared to the peripheral retina and to the posterior pole retina+peripheral retina). Conclusions: Compr-ehensive treatment improved the survival and eyeball preservation rate of children with RB. The survival rates and eyeball preservation rates were closely related to the clinical stage.

Objective To observe the effect of systemic chemotherapy on conditions of tumor infiltrating,metastasis and disease-specific survival (DSS) for advanced retinoblastoma (RB).Methods Forty-one patients with advanced RB who received enucleation were enrolled in this study.There were 26 males and 15 females,age at diagnosis was ranged from 2 to 72 months,with a mean of 23.08 months.There were 16 bilateral patients and 25 unilateral patients;13 group D eyes and 28 group E eyes.16 patients received enucleation as the primary treatment (operation group),25 eyes received chemotherapy before enucleation (chemotherapy group).There was no significant statistical difference between two groups for the gender,unilateral and bilateral,international staging or diagnostic age (P＞0.05).The histopathology report was performed to assess the risk of postoperative tumor-node-metastasis staging (pTNM) in each patient,and the extent of tumor invasion in the optic nerve,choroid and anterior chamber was divided into 3 levels of low risk,medium risk and high risk.Five deaths were all in the group E with chemotherapy before enucleation.Using R software survival analysis software package survfit function,the application of Kaplan-Meier estimation method,DSS of RB children was calculated from the time of diagnosis,up to the date of the death of patient.DSS differences between chemotherapy,operation group and eye removal time (more than 3 months,less than 3 months) in group E RB children were analyzed.Results The proportion of high risk pTNM stage in chemotherapy group was significantly lower than the operation group.But there was no significant difference between the two groups in the overall risk classification (x2 =3.130,P=0.077).For group D eyes,the overall risk classification in chemotherapy group was significantly lower than the operation group (x2 =5.870,P=0.015).There was no significant difference between the two groups in the overall risk of group E eyes (x2 =0.020,P=0.889).The DSS in chemotherapy group and operation group were 0.71 and 1.00,respectively;the difference was significant (x2 =3.700,P=0.05).The DSS in children whose enucleation delayed for more than 3 months and children whose enucleation performed within 3 months were 0.64 and 1.00,respectively;the difference was significant (x2 =4.800,P=0.028).Conclusion Systemic chemotherapy did not reduce the risk of tumor invasion and metastasis in patients with advanced RB.Instead,it will reduce the DSS in group E eyes of RB.

观察在两种模式下RetCamⅢ（数字视网膜成像系统）对早产儿视网膜病变筛查的效果。将我院运用RetCamⅢ进行ROP筛查早产儿956例，随机按筛查模式分为眼科中心模式（A组）和NICU模式（B组），比较两组筛查率、筛查完成率、初筛及筛查完成满意度及筛查过程并发症发生率差异；结果B组筛查完成率、初筛满意度、完成筛查满意度及患儿并发症总发生率均低于A组（P<0．05）。 NICU模式下运用RetCamⅢ进行ROP筛查，较眼科中心模式更安全、直观、有效，值得临床推广。

Objective To analyze the incidence of retinopathy of prematurity(ROP)among extreme-ly preterm infants,and to evaluate the treatment methods and effects among those with severe ROP.Methods A retrospective analysis was performed to analyze incidence of ROP in 96 cases of extremely preterm infants who were born at a gestational age of 〈28 weeks and survived beyond a postmenstrual age of at least 1 year from Apr 2006 to Oct 2013,and to analyze the treatment outcomes of photocoagulation and ranibizum-ab intravitreal injection among the infants with severe ROP.Results Fifty-six of 96 cases(58.33%)grew into ROP finally and 21 cases(21.88%)grew into severe ROP,2 cases(2.08%)grew into aggressive poste-rior ROP.Fifteen cases with severe ROP were treated with laser photocoagulation.Four cases with severe ROP were received ranibizumab intravitreal injection prior to photocoagulation.Two cases with severe ROP were only treated with ranibizumab intravitreal injection.The eyesight of 96 patients (100%)in this study were all preserved.Conclusion ROP screening should focus on extremely preterm infants because of higher incidence of ROP and severe ROP among them.The infants with severe ROP should be treated with laser photocoagulation in time.The infants in critical condition or with aggressive posterior ROP can be treated with ranibizumab injection.

目的：利用双生子人群探讨中国汉族人口ROP（retinopathy of prematurity，ROP）的遗传度。方法以2005年1月至2011年12月于我院新生儿科就诊的胎龄＜34周，出生体质量＜2000 g并存活至矫正胎龄为40周后的汉族双胞胎早产儿321对为研究对象。根据卵型不同分为同卵双胎（monozygotic twins，MZ）组123对和异卵双生子（dizygotic twins，DZ）组198对。记录所有对象的胎龄、出生体质量、性别、是否合并ROP、肺透明膜病（RDS）、ROP、窒息、贫血、氧疗时间、机械通气时间等临床资料，在纠正胎龄32周或生后4～6周时，由眼科医师用间接眼底镜进行ROP筛查，采用统计软件SPSS19.0软件包和Mx双生子统计软件包，对所有临床资料进行回顾性分析。结果中国汉族人口中，ROP的遗传度为68.43%。结论中国汉族人口中，遗传是ROP的主要发病因素。

OBJECTIVE To observe the retinal vascular development and changes on aggressive posterior retinopathy of prematurity (AP-ROP) by intravitreal ranibizumab, evaluate the therapeutic effect, and provide the basis for clinical treatment. METHODS The total of 38 eyes of 19 premature infants who were diagnosed as AP-ROP from January 2012 to October 2013 in our hospital were performed intravitreal injection of ranibizumab (0.04 ml). It was observed about the regression of plus diseases, ridges, neovascularization on the ridge and the development of peripheral retinal vessel. Laser photocoagulation were performed for 14 eyes of 7 cases whose plus diseases, ridges and neovascularization on the ridge didn't regress completely after intralvitreal injection of ranibizumab. RESULTS All infants were found retinopathy regressed and retinal vessels continued to develop peripherally to some degree. Of all infants, 24 eyes of 12 infants were found complete regression of retinopathy, resolution of neovascularization and bleeding and that retinal vessels continued to develop to ora serrata or scarification of peripheral retinopathy. Fourteen eyes of 7 infants were found retinopathy didn't regressed completely and regressed completely after combing intravitreal ranibizumab injection. All 19 infants didn't occure infection, ocular or systemic complications. CONCLUSIONS The efficacy of intravitreal injection of ranibizumab is good for AP-ROP. It can made ridge, neovascularization on the ridge and plus disease regress, as well as let the retinal vessel continue development. Infants with no regressed retinopathy may need combined laser photocoagulation.

Objective To investigate the effect of intravitreous injection of Avastin for cystoid macular edema after cataract surgery.Methods Eleven cases with cystoid macular edema after cataract surgery were performed intravitreous injection of Avastin(1.5mg)and follow up 3 months.The pre-operative and post-operative best corrected visual acuity(BCVA),intraocular pressure(IOP),slim lamp and fundus examination were recorded and analyzed at different time point.At the same time,optical coherence tomography(OCT)examinations were performed.Results The pre-op BCVA in all cases were improved after intravitreous injection of Avastin.During the follow-up,no significant changes in IOP and anterior segment.The retina toxicity reactions relevant to Avastin were nothing.The pre-injection central macular thickness was(563.37±147.86)μtm,while the post-op central macular thickness was(208.47±86.34)μm at 1 month post-injection and(202.12±78.27)μm at 3 months post-injection.There was significant difference between the thickness pre-injection and post-injection.Conclusions The intravitreous injection of Avastin can relieve macular edema and improve BCVA for cystoid macular edema after cataract surgery.

目的 探讨玻璃体手术治疗眼内晚期视网膜母细胞瘤的效果.方法 2012年7月至2013年6月,选择眼内晚期(D期或E期)视网膜母细胞瘤患儿11例(12眼),采用博士伦23G玻璃体切割系统,进行玻璃体视网膜手术,包括玻璃体切割、肿瘤切除、视网膜光凝、眼内填充等.手术后随访6～18个月(平均9.4个月).结果 所有患眼均完成视网膜肿物切除.术后10眼视网膜裂孔封闭、视网膜复位;2眼因肿瘤范围大,视网膜大部分切除.8眼硅油填充,2眼行C3F8填充,2眼未作眼内填充.随访末,病情稳定者8眼,肿瘤复发4眼,眼球摘除1眼,因肿瘤眼外转移去世1例,2眼再行玻璃体内注射马法兰.硅油取出3眼,硅油置换1眼,4眼硅油长期填充.视力保持0.2以上5眼,患儿因年龄小不配合视力检查者7眼.并发白内障2眼,行白内障摘出术.结论 玻璃体手术能够有效切除眼内晚期视网膜母细胞瘤,对保留眼球和患眼视力,提高患儿生存率有一定帮助,但其安全性和长期疗效还有待进一步研究.

AIM: To observe the treatment of anterior chamber paracentesis for elevated intraocular pressure(IOP) in scleral buckling. METHODS: Totally 28 cases 28 eyes with phakic rhegmatogenous retinal detachment who have elevated IOP in the scleral buckling were observed and analyzed; In operation, all patients underwent subretinal fluid draining, positioning, retinal breaks condensing, and given segmental scleral pressure or combined with scleral buckling under direct vision with binocular indirect ophthalmoscope; Before the end of operation, patients with the fundus examination revealed retinal arterial pulse, high IOP were given anterior chamber paracentesis fluid therapy. RESULTS: In the first day after surgery, all patients were given IOP checks, the result of all patients were with IOP less than 21mmHg, and no corneal edema, shallow anterior chamber and retinal artery occlusion etc complications. CONCLUSION: Anterior chamber paracentesis is a simple, easy, safe and effective treatment method for high IOP caused by scleral buckling.

Objective This study was to analyze the outcomes of local treatment of early retinoblastoma(Rb) with the use of wide field digital retinal imaging system.Methods The clinical data of 12 cases(12 eyes) with retinoblastoma(low risk,A/B stage) confirmed between 2009 and 2012 were retrospectively analyzed.The patients underwent several topical ophthalmic therapies including laser and cryotherapies.Results The survival rate and rate of eyeball salvage were 100% in both stage A and stage B Rb.Tumors were atrophied and calcified in all cases and no recurrence,serious ocular complications or systemic complications were found.Conclusion The early diagnosis is very important for the successful treatment of retinoblastoma.Pediatric wide field digital retinal imaging system(RetCam) facilitates tropical treatment such as laser coagulation or cryotherapies,and is helpful to increase the rate of successful treatment in retinoblastoma.

Objective To analyze and evaluate the screening and treatment outcomes of extremely low body weight below 1 000 g newborn with retinopathy of prematurity(ROP).Methods Totally 217 premature infants who were body weight ≤1 000 g at birth,were enrolled in this study.Their fundus were routinely checked by binocular indirect ophthalmoscope and RetCam II.Diagnosis and staging of ROP were performed according to the international guidelines.The screening and treatment outcomes of ROP were statistically analyzed.Results The incidence of ROP was 60.8%(132 patients),and other ocular pathological changes in 10 cases(4.6%).96 patients(72.7%) suffered from ROP were treated by the several surgical methods,including retinal photocoagulation in 66 cases,vitreous injection of combined photocoagulation in 20 cases,vitreous injection in 4 case and vitrectomy in 6 cases.Conclusion The incidence of ROP to extremely low body weight infants is much higher than that of the 1 000 g above newborns.The patient has a high probability of surgical treatment,and often need to join a variety of treatment interventions.

目的:分析双生子新生儿的早产儿视网膜病变发病情况和临床特点.方法:回顾性分析2008年6月至2012年6月在我院筛查眼底的体重2 000 g以内(合2 000 g)的患儿,采用广域数字化小儿视网膜图像系统(RectcamⅡ)或双目间接眼底镜对其进行眼底检查,观察并分析双生子与单生子ROP发生的临床特点与相关因素.结果:纳入研究的早产儿总计4 855例,其中双生子874例,单生子3 981例.男2 660例,女2195例,出生胎龄25～ 40周,平均33.76周,出生体重680 ～2 000 g,平均1 415 g.所有患儿中有ROP病变的675例,总患病率为13.9％.双生子ROP的发病率高于单生子(P＜0.05)；双生子在体重与胎龄方面与单生子相比差异有显著性.结论:由于双生子的胎龄小,体重低,其ROP的发病率明显高于单生子且发病时间较早,提示对于多胞胎眼底情况需要密切关注,防范ROP带来的医疗风险的发生.