Background:Severe delayed encephalopathy after acute carbon monoxide poisoning (s-DEACMP) is a disabling complication associated with substantial long-term neurological impairment, yet reliable predictors for risk stratification during acute hospitalization remain limited. This study aimed to identify predictors associated with s-DEACMP and to develop a preliminary predictive model. Methods:In this retrospective single-center cohort study, 200 patients with acute carbon monoxide poisoning (ACMP) admitted between 2017 and 2024 were analyzed. Patients were categorized into DEACMP (n = 140) and non-DEACMP (n = 60) groups; the DEACMP group was further stratified into severe (s-DEACMP, n = 97) and mild-to-moderate (m-DEACMP, n = 43) subgroups based on Activities of Daily Living scores assessed at peak disease severity during hospitalization. Clinical characteristics were compared between the s-DEACMP group and a combined control group (n-DEACMP + m-DEACMP). Independent predictors were identified using multivariate logistic regression. A nomogram was constructed and internally validated using receiver operating characteristic (ROC) curves, calibration analysis, and decision curve analysis (DCA). Results:Patients with s-DEACMP were significantly older and had longer duration of disturbance of consciousness, higher prevalence of hypertension and hyperhomocysteinemia, elevated D-dimer levels, and lower serum albumin concentrations compared with controls (all P < 0.05). Multivariate analysis identified age >40 years (OR = 31.90, 95% CI = 3.93-259.14), disturbance of consciousness >24 h (OR = 3.06, 95% CI = 1.58-5.94), hypertension (OR = 1.99, 95% CI = 1.02-3.90), and hyperhomocysteinemia (OR = 2.57, 95% CI = 1.24-5.31) as factors independently associated with s-DEACMP. The nomogram demonstrated acceptable discrimination and calibration. Conclusion:The proposed nomogram may assist in identifying patients at increased risk for s-DEACMP during acute hospitalization. External multicenter validation is required before broader clinical application.

Hyperbaric oxygen (HBO) suppresses the inflammatory response following spinal cord injury (SCI). However, the underlying detailed mechanisms are still to be clarified. Here we explored the mechanism of long non-coding RNA (lncRNA) glutathione s-transferase mu 5 (Gstm5) regulating NF-κB signaling pathway in HBO-mediated suppression of inflammatory response following SCI. In the current study, SCI cell model was developed with lipopolysaccharides (LPS)-induced BV2 cells and processed with HBO treatment, si-NC, and si-Lnc-Gstm5. Lnc-Gstm5, NF-κB p65, IL-1β，IL-6, TNF-a, suppressor of variegation 3-9 homolog 1 (SUV39H1), histone 3 lysine 9 trimethylation (H3K9me3), YTH domain containing 2 (YTHDC2) expression level were measured. The mice SCI model was generated and treated with HBO treatment, shRNA-Lnc-Gstm5. Lnc-Gstm5 was identified and BMS score, histopathological injury score, and inflammatory factors were evaluated. We found that HBO suppresses inflammatory response through up-regulating Lnc-Gstm5 level in a manner of YTHDC2-dependent m6A modification. Lnc-Gstm5 recruits SUV39H1 to up-regulate H3K9me3 expression level and suppresses NF-κB signaling pathway by reducing p65 phosphorylation. HBO suppresses the inflammatory response via YTHDC2/Lnc-Gstm5/SUV39H1/H3K9me3/NF-κB axis following SCI in mice. These results reveal a Lnc-Gstm5-driven epigenetic regulation mechanism, and targeting Lnc-Gstm5 represents a promising therapeutic strategy for SCI patients.

Common cardiovascular surgeries include coronary artery bypass grafting, cardiac valve replacement, radiofrequency ablation, and cardiac intervention surgery. Multiple postoperative complications, such as hypoxic encephalopathy, air embolism, retained intracardiac air, cognitive dysfunction and major adverse cardiovascular events, including heart failure, ischemic stroke, and myocardial infarction, may occur after these cardiovascular surgeries. Hyperbaric oxygen can be used in preconditioning to lower the morbidity of adverse complications. It is also effective for the treatment of numerous postoperative complications. We provide evidence from the current literature highlighting the use of hyperbaric oxygen therapy for preconditioning and managing postoperative complications.

Hyperbaric oxygen (HBO) treatment can attenuate the inflammatory response after spinal cord injury (SCI), but the specific molecular mechanisms are unclear. γδT cells have emerged as key immune regulatory cells. This study aims to analyze the mechanism of HBO treatment reducing SCI mainly from the perspective of γδT cells. Contusion SCI models were established in WT, TCRδ−/−, and IL-17−/− mice, and HBO treatment was performed. Hindlimb locomotor function, ChAT-positive motor neuron numbers, γδT cell proportion, and inflammatory cytokines level of different experimental groups were compared after SCI. Furthermore, we measured the protein and gene expression of STAT3 and RORγt in both WT mice and STAT3 overexpression mice following HBO treatment. Our results showed γδT cells, especially IL-17 + γδT cells, were involved in SCI. HBO treatment improved locomotor recovery, reduced the proportion of γδT and IL-17 + γδT cells, and decreased inflammatory cytokines in WT mice after SCI. However, HBO treatment did not affect locomotor recovery or inflammatory cytokines level in TCRδ−/− or IL-17−/− mice after SCI. In addition, HBO treatment significantly inhibited the expression of STAT3 and RORγt in WT mice after SCI. Over-expression of STAT3 weakened the inhibitory effect of HBO on IL-17 + γδT cells and inflammatory cytokines after SCI. These findings indicated that IL-17 + γδT cells may play a key role in HBO treatment alleviating the inflammatory response after SCI. HBO treatment may regulate IL-17 + γδT cells by modulating the pathway of STAT3/RORγt. This study provides the theoretical basis and potential therapeutic target for HBO treatment in SCI.

Gut dysbiosis impacts the recovery of neurological function after spinal cord injury (SCI). Hyperbaric oxygen (HBO) can alleviate SCI, but its effects on the gut microbiota post-SCI remain unclear. This study aimed to clarify the impact of HBO on SCI-induced gut dysbiosis and to explore the mechanisms of locomotor recovery in HBO-treated SCI mice. After establishing different groups of mouse models, bacterial cultures and Basso Mouse Scale (BMS) scores were performed at various time points post-SCI. Intestinal tissues were collected for intestinal permeability assay, histological analysis, immunofluorescence, and qPCR analysis. Flow cytometry and ELISA were used to detect immune-inflammatory cells and cytokines in intestinal tissue. The composition of gut microbiota in fecal samples from each group was also analyzed. Spinal cord tissues were collected for immunofluorescence and untargeted metabolomics analysis. Spearman correlation analysis was used to correlate differential microbiota with differential metabolites. Our results showed that the expression of tight junction proteins was increased after HBO treatment in SCI mice. Metagenomic analysis of the fecal DNA revealed that HBO altered intestinal bacterial composition. Differential metabolites were mainly enriched in pathways, such as glycerophospholipid metabolism, steroid biosynthesis, and glycolysis/gluconeogenesis. Moreover, differential microbiota showed a strong correlation with differential metabolites related to glycerophospholipids. HBO treatment significantly inhibited immune cells and inflammatory cytokines in the gut after SCI. In addition, HBO treatment significantly increased BMS scores and body weight, and repaired damaged cholinergic neurons. Antibiotic-induced gut dysbiosis impaired the recovery of locomotor function and exacerbated intraspinal pathology. However, these effects could be mitigated by HBO treatment. Overall, HBO treatment may improve neurological recovery through multiple regulatory mechanisms including alleviating gut dysbiosis, reducing intestinal inflammation, and rectifying glycerophospholipid metabolic disorders after SCI. These findings highlight HBO as a promising therapeutic strategy for SCI treatment and support its clinical application. KEY MESSAGES: The intestinal microbiota composition of mice changed after SCI. HBO treatment could preserve intestinal barrier integrity, modulate the composition of intestinal microbiota, rectify glycerophospholipid metabolic disorders, and reduce intestinal immune inflammatory responses. Intestinal microbiota identified as the target for HBO therapeutic in SCI recovery. Alleviating SCI-induced gut dysbiosis may be one of the mechanisms underlying the beneficial effect of HBO on neurological functions.

JOURNAL/mgres/04.03/01612956-202603000-00002/figure1/v/2025-06-28T140100Z/r/image-tiff Paroxysmal sympathetic hyperactivity syndrome (PSH) is common in patients with severe craniocerebral injuries. Carbon monoxide poisoning (ACOP) may lead to secondary PSH, and hyperbaric oxygen (HBO) is an important treatment method for ACOP that can promote the dissociation of carboxyhemoglobin and reduce the long-term sequelae of ACOP. To explore the risk factors and clinical characteristics of PSH secondary to acute ACOP and to investigate the efficacy of HBO treatment, a retrospective analysis was performed on patients with moderate to severe ACOP admitted to the Hyperbaric Oxygen Department of Beijing Chaoyang Hospital, Capital Medical University, from January 1, 2018 to December 31, 2024. Three patients developed PSH during hospitalization and were classified into the PSH group, while the remaining 50 patients were in the non-PSH group. Univariate Fisher's exact test indicated that a coma duration of more than 72 hours was related to the occurrence of PSH after ACOP, and irregular HBO treatment after onset might be associated with the occurrence of PSH after ACOP. All three PSH patients developed paroxysmal postural or dystonic disorders after onset, accompanied by sympathetic excitation manifestations such as increased heart rate, respiratory rate, elevated blood pressure, and fever. Antiepileptic drugs had poor effects, and the attacks were effectively controlled after HBO treatment combined with adjusted drug therapy. The results indicate that for patients with severe carbon monoxide poisoning, especially those with a long coma duration or irregular HBO treatment after onset, if epileptic seizures occur during the disease course and are accompanied by sympathetic excitation manifestations, the possibility of PSH should be considered. Regular HBO treatment is of great significance for controlling the onset of symptoms.

While diosgenin has been demonstrated effective in various cardiovascular diseases, its specific impact on treating heart attacks remains unclear. Our research revealed that diosgenin significantly improved cardiac function in a myocardial infarction (MI) mouse model, reducing cardiac fibrosis and cell apoptosis while promoting angiogenesis. Mechanistically, diosgenin upregulated the Hand2 expression, promoting the proliferation and migration of endothelial cells under hypoxic conditions. Acting as a transcription factor, HAND2 activated the angiogenesis-related gene Aggf1. Conversely, silencing Hand2 inhibited the diosgenin-induced migration of hypoxic endothelial cells and angiogenesis. In summary, these findings provide new insights into the protective role of diosgenin in MI, validating its effect on angiogenic activity and providing a theoretical basis for clinical treatment strategies.

STUDY DESIGN:A functional, transcriptome, and long noncoding RNAs (lncRNAs) expression analysis in the spinal cord of mice after hyperbaric oxygen (HBO) treatment.OBJECTIVE:We aimed to explore the mechanism by which HBO treats spinal cord injury (SCI) at the level of lncRNAs.SUMMARY OF BACKGROUND DATA:Immense amounts of research have established that HBO treatment promotes the recovery of neurological function after SCI. The mechanism of action remains to be clarified.METHODS:High-throughput RNA sequencing, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were used to profile lncRNA expression and analyze biological function in the spinal cords of mice from sham-operated, SCI, and HBO-treated groups. The differential expression of lncRNA between the groups was assessed using real-time quantitative polymerase chain reaction.RESULTS:Differential expression across 577 lncRNAs was identified among the three groups. GO analysis showed that free ubiquitin chain polymerization, ubiquitin homeostasis, DNA replication, synthesis of RNA primer, single-stranded telomeric DNA binding, and alpha-amylase activity were significantly enriched. Kyoto Encyclopedia of Genes and Genomes enrichment analysis displayed that vitamin B6 metabolism, one carbon pool by folate, DNA replication, lysine degradation, beta-alanine metabolism, fanconi anemia pathway, and Notch signal pathway were the main pathways with enrichment significance. LncRNAs NONMMUT 092674.1, NONMMUT042986.2, and NONMMUT018850.2 showed significantly different expression between the SCI and the other two groups (P<0.05, <0.01).CONCLUSIONS:This study is the first to determine the expression profiles of lncRNAs in the injured spinal cord after HBO treatment. We identified several important dysregulated lncRNAs in this setting. These results help us better understand the mechanism by which HBO treats SCI and provide new potential therapeutic targets for SCI.

Study Design. Basic science study investigating the potential molecular mechanisms of hyperbaric oxygen (HBO) therapy in mice with spinal cord injury (SCI).Objective. We aimed to explore the intrinsic mechanisms of HBO for SCI through the lens of ferroptosis in the subacute phase.Summary of Background Data. HBO has been observed to facilitate the restoration of neurological function subsequent to SCI. Ferroptosis is a distinct cellular death mechanism that can be distinguished from apoptosis, necrosis, and autophagy. However, the precise relationship between these two phenomena remains poorly understood.Methods. We established an SCI model and employed a range of techniques, including behavioral assessments, electron microscopy, immunofluorescence, RT-qPCR, Western blotting (WB), Glutathione (GSH) measurement, and iron assay, to investigate various aspects of HBO therapy on SCI in mice. These included analyzing mitochondrial morphology, neuronal count, GSH levels, iron levels, and the expression of genes (Acyl-CoA synthetase family member-2, Iron-responsive element-binding protein-2) and proteins (Glutathione peroxidase 4; system Xc-light chain) associated with ferroptosis. The study included three groups: Sham-operated, SCI, and HBO. Group comparisons were performed using one-way analysis of variance and one-way repeated measures analysis of variance, followed by Tukey's post hoc test. Statistical significance was set at a P < 0.05.Results. Our findings revealed that HBO therapy significantly enhanced the recovery of lower limb motor function in mice following SCI in the subacute phase. This was accompanied by upregulated expression of GPX4 and system Xc-light chain proteins, elevated GSH levels, increased number of NeuN+ cells, decreased expression of the iron-responsive element-binding protein-2 gene, and reduced iron concentration.Conclusions. Our research suggests that HBO therapy has the potential to be an effective treatment for SCI in the subacute phase by mitigating ferroptosis.

Objective:To explore the changes in serum neuron-specific enolase（NSE）in patients with moderately severe to profound idiopathic sudden sensorineural hearing loss（ISSHL）and the effect of hyperbaric oxygen（HBO）therapy on NSE，and to analyze the relationship between hearing recovery and serum NSE levels.Methods:Using convenient sampling，a total of 90 inpatients with unilateral moderately severe to profound ISSHL were selected as the research subjects，and they were divided into observation group and control group according to the random number table method，with 45 cases in each group. The control group received hormones，Ginkgo biloba injection，and neurotropic drugs，and the observation group was treated with HBO based on the treatment of the control group. Pure tone audiometry was performed before and after treatment to test hearing levels，and serum NSE was determined by ELISA. After 20 days of treatment，the effective rates and the changes of NSE of the two groups were compared，and the relation between NSE level and hearing change were analyzed.Results:The serum NSE levels of ISSHL patients in both groups were significantly higher than those of the control group，and it was correlated with the severity of hearing loss（ P<0.01）. The serum NSE levels of profound ISSHL patients were significantly higher than those of moderately severe and severe ISSHL patients（ P<0.01）. After treatment，the clinical effective rate of the observation group（82.2%）was significantly higher than that of the control group（60.0%）（ P<0.05）；the hearings of the patients in both groups were significantly improved compared with those before treatment；the pure tone average of the observation group was lower than that of the control group（ P<0.05）；and the hearing threshold gain of the observation group was significantly higher than that of the control group（ P<0.01）. After treatment，the NSE levels of the patients in the two groups decreased after treatment；the NSE level of the observation group was significantly lower than that of the control group（ P<0.05）；and the decrease of NSE level in the observation group was significantly greater than that of the control group（ P<0.01）. The decreases of serum NSE in the two groups were positively correlated with the gains of the auditory domain（ r=0.686， P<0.01 in the observation group； r=0.418， P<0.01 in the control group）. Conclusion:The serum NSE level of patients with moderately severe to profound ISSHL is increased，which is related to the severity of hearing loss. Additionally adopting HBO therapy could improve the hearing of patients，increase the effective rate，and reduce the NSE level of ISSHL patients.

目的 探讨PBL教学法在高压氧医学科教学查房中的应用效果,探索更符合高压氧医学科特点的教学查房模式.方法 选取2021年3月1日-2021年5月31日在某院高压氧科进行临床实习的实习医师32人,采用信封法随机分为2组.实验组16人,采用以问题为基础的教学查房,对照组16人,采用传统教学查房模式.通过考核及问卷调查评价教学效果.结果 实验组病例分析成绩高于与对照组(分别为88.4±3.5分和78.8±5.2分),差异具有统计学意义(P<0.05),基础知识成绩2组间差异无统计学意义,分别为(85.9±3.6)分和(84.1±4.3)分,P>0.05.75％的实习生对PBL教学模式总体评价为很满意或比较满意.结论 PBL教学查房可以提高实习医师病例分析能力、锻炼临床思维,促使实习医师更好的掌握高压氧科常见疾病的诊治.

Abstract Background β2-microglobulin has been showing to be vital that associated with brain function and neurological diseases. This study aimed to explore the expressions of β2-microglobulin in blood and urine of the patients with brain injury, and the effect of hyperbaric oxygen therapy on the content of β2-microglobulin. Methods This prospective study included 54 patients with brain injury and 11 healthy controls. The patients were further assigned to two groups: the conscious disturbance group (n = 32) and the non-conscious disturbance group (n = 22) depending on the Glasgow Coma Scale (GCS). The patients received routine treatment and two courses of hyperbaric oxygen therapy (2.0ATA, 60 min, once a day, 10 days for a course). In the brain injury group, blood β2-microglobulin (β2MG) and urine β2-microglobulin (β2MU) were detected respectively before and after hyperbaric oxygen therapy (HBOT). Consciousness and cognitive scores were performed, correspondingly. Results Compared with those of the control group, levels of β2MG and β2MU in the brain injury group were significantly increased before HBOT (P < 0.05). Whether it was before or after HBOT, β2MG’s content in the conscious disturbance group was higher than that in the non-conscious disturbance group, while β2MU’s content was obviously higher than that of the non-conscious disturbance group only before HBOT (P < 0.05). Besides, the β2MU’s content in the conscious disturbance group was negatively correlated with GCS score (R = -0.351, P < 0.05) and β2MG’s content in the non-conscious disturbance group was positively correlated with the MMSE score grade (R = 0.598, P < 0.05). The ROC curve was used to assess the evaluation of β2MG and β2MU for patients with impaired consciousness with the area under the curve (AUC) of β2MG and β2MU were 0.775 and 0.796, respectively. Conclusion The concentrations of blood β2-microglobulin and urinary β2-microglobulin were significantly increased in patients with brain injury. The concentrations of β2-microglobulin were correlated with the degree of consciousness and cognitive function. The changes tendency of β2-microglobulin may be considered as clinical monitoring index to evaluate the patient’s disturbance of consciousness and cognitive degree, and provide a basis for early assessment of prognosis.

Accumulating studies have demonstrated that hyperbaric oxygen (HBO) treatment alleviates spinal cord injury (SCI). However, the underlying mechanism by which HBO alleviates SCI remains to be elucidated. In this study, we performed genome-wide transcriptional profiling of the spinal cord between SCI mice and mice that received HBO treatment by high-throughput RNA sequencing at 1 week after SCI. We also compared genome-wide transcriptional profiles from SCI mice and sham-operated mice. We found 76 differentially co-expressed genes in sham-operated mice, SCI mice, and HBO-treated SCI mice. Using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis, we identified the biological characteristics of these differentially expressed genes from the perspectives of cell component, biological process, and molecular function. We also found enriched functional pathways including ferroptosis, calcium signaling pathway, serotonergic synapse, hypoxia-inducible factor-1 signaling pathway, cholinergic synapse, and neuroactive ligand-receptor interaction. We performed quantitative reverse transcription-polymerase chain reaction and validated that HBO treatment decreased the expression of Hspb1 (heat shock protein beta 1), Hmox1 (heme oxygenase 1), Ftl1 (ferritin light polypeptide 1), Tnc (tenascin C) and Igfbp3 (insulin-like growth factor binding protein 3) and increased the expression of Slc5a7 (solute carrier family 5 choline transporter member 7) after SCI. These results revealed the genome-wide transcriptional profile of the injured spinal cord after HBO treatment. Our findings contribute to a better understanding of the mechanism by which HBO treats SCI and may provide new targets for SCI intervention.

Objective:Hyperbaric oxygen therapy (HBOT) has been recommended for the initial and salvage treatment of patients with idiopathic sudden sensorineural hearing loss (ISSHL), but its underlying mechanisms remain unclear. In this study, we investigated whether HBOT alters serum levels of insulin-like growth factor 1 (IGF-1) and heat shock protein 70 (HSP70) in patients with ISSHL. Then, we identified the relationship between hearing recovery and changes in serum IGF-1 and HSP70 levels.Methods:Moderately severe to profound unilateral ISSHL patients (n = 70) and healthy control participants (n = 30) were enrolled. The ISSHL patients were randomly assigned to receive medical therapy alone (MT group, n = 35) or both HBOT and medical therapy (HBOT + MT group, n = 35). Audiometric testing was performed before and after treatment. Serum IGF-1 and HSP70 levels were assessed by ELISA in ISSHL patients pre-and posttreatment and healthy controls.Results:Before treatment, compared with the healthy controls, serum IGF-1 and HSP70 were lower in ISSHL patients. After treatment, serum IGF-1 and HSP70 increased in both the HBOT + MT and MT groups, although they were significantly higher in the HBOT + MT group (p < 0.01). In the HBOT + MT group, these increases were associated with hearing gains. In addition, IGF-1 was strongly associated with HSP70 (r = 0.621, p = 0.001). No such association was found in the MT group (p = 0.757).Conclusion:Administering HBOT in addition to medical therapy can improve the hearing of patients with moderately severe to profound unilateral ISSHL. The improvement is related to the upregulation of IGF-1 and HSP70.

Hyperbaric Oxygen Therapy (HBOT) has definitive therapeutic effects on spinal cord injury (SCI), but its mechanism of action is still unclear. Here, we've conducted a systemic proteomic analysis to identify differentially expressed proteins (DEPs) between SCI rats and HBOT + SCI rats. The function clustering analysis showed that the top enriched pathways of DEPs include oxygen transport activity, oxygen binding, and regulation of T cell proliferation. The results of functional and signal pathway analyses indicated that metabolic pathways, thermogenesis, LXR/RXR activation, acute phase response signaling, and the intrinsic prothrombin pathway in the SCI + HBOT group was higher than SCI group.

Objective:To investigate the effect of hyperbaric oxygen (HBO) therapy on microglia polarization after traumatic brain injury (TBI) in rats.Methods:A total of 168 8-week-old SD rats were randomly divided into sham operation (SH) group, sham operation+ HBO (SH+ HBO) group, traumatic brain injury (TBI) group, and traumatic brain injury+ HBO (TBI+ HBO) group, with 42 rats in each group. The TBI rat model and sham operation rat model were established. The observation time points of each group were 1 h, 6 h, 12 h, 24 h, 72 h, 7 d and 14 d. The SH+ HBO group and the TBI+ HBO group were treated with HBO. The modified neurological severity score (mNSS) was used to evaluate the recovery of the neurological function in TBI rats. After taking the injured brain tissues from the decapitated rats, the inducible nitric oxide synthase (iNOS), a microglia marker, and chitinase 3-like 3 (YM1) were detected by Western blotting. The expression levels of IL-6 and IL-10 were detected by ELISA.Results:Compared with the TBI group, mNSS in the TBI+ HBO group was decreased significantly at 7 d and 14 d after injury ( P<0.05). Compared with the SH group and the SH+ HBO group, the expression levels of iNOS protein in brain tissues of the TBI group and the TBI+ HBO group were significantly increased at 24 h, 72 h, and 7 d after TBI ( P<0.05). Compared with the TBI group, the expression levels of iNOS protein in brain tissues of the TBI+ HBO group were significantly decreased at 72 h and 7 d after TBI ( P<0.05). Compared with the SH group and the SH+ HBO group, the expression levels of YM1 protein in brain tissues of the TBI group and the TBI+ HBO group were significantly increased at 6 h, 12 h, 24 h, and 72 h after TBI ( P<0.05); compared with the TBI group, the expression levels of YM1 protein in brain tissues of the TBI+ HBO group were significantly increased at 12 h and 24 h after TBI ( P<0.05). Compared with the SH group and the SH+ HBO group, the expression levels of IL-6 in the TBI group and the TBI+ HBO group were increased significantly after TBI ( P<0.05); compared with the TBI group, the expression levels of IL-6 in the TBI+ HBO group were decreased significantly at 24 h, 72 h, and 7 d after TBI ( P<0.05). Compared with the SH group and the SH+ HBO group, the expression levels of IL-10 in the TBI group and the TBI+ HBO group were significantly increased after TBI ( P<0.05); compared with the TBI group, the expression levels of IL-10 in the TBI+ HBO group were significantly increased at 24 h and 72 h after TBI ( P<0.05). Conclusion:HBO treatment can effectively improve the hypoxic-ischemic state in injured brain tissues of TBI rats, promote the restoration of blood-brain barrier, facilitate the repopulating of M2 type microglia, and promote the polarization of microglia from M1 to M2 in the early stage of brain injury.

目的 探讨基于微信平台实施混合教学在住院医师规范化培训高压氧科轮转中的应用价值.方法 于2017年4月—2019年12月纳入65位进行高压氧科轮转的住院医师在教学方面开展研究.该模式贯穿整个轮转过程并建立了反馈机制.分析入科时不同学历的住院医师对高压氧医学的了解程度,统计出科考核成绩、轮转收获.结果 不同学历的住院医师对高压氧医学了解程度差异无统计学意义(P=0.926).65位轮转医生都顺利通过出科理论考核,考核成绩(87.9±4.3)分,3位医生补考神经系统查体.根据反馈,轮转医师在高压氧科收获最大的两项是:了解高压氧的适应证、禁忌证及高压氧治疗的过程(56/65).12位轮转医师为我们的微信公众号撰写了轮转体会.结论 该教学模式帮助带教老师了解轮转医师的需求、利于个性化制定轮转计划,激发了轮转医师主动学习高压氧知识的兴趣和动力、提高了参与度,增强了师生之间的互动,提升了教学效果.

Disruption of the intestinal epithelial barrier following spinal cord injury (SCI) seriously affect long-term quality of life. Oxidative stress-induced epithelial cells' injury contributes to the epithelial barrier dysfunction. Hyperbaric oxygen (HBO) treatment has been proved to alleviate SCI. However, it is unclear whether or not HBO treatment affects intestinal barrier function following SCI. In this study, our purpose was to explore the impact of HBO treatment on intestinal epithelial barrier function and underlying mechanisms following SCI. An SCI model was established in rats, and the rats received HBO treatment. Intestinal injury, mucosal permeability, intercellular junction proteins, and oxidative stress indicators were evaluated in our study. We found that HBO treatment significantly alleviated intestinal histological damage, reduced mucosal permeability, and markedly prevented bacterial translocation. Furthermore, HBO treatment significantly increased the expression of Claudin-1 and E-cadherin, inhibited intestinal tissue oxidative stress as demonstrated by upregulation of superoxide dismutase and glutathione, and HBO downregulated malondialdehyde. Mechanically, we demonstrated that HBO treatment ameliorated intestinal oxidative stress possibly through upregulating nuclear factor E2-related factor 2 (Nrf2) and its downstream targets, Heme oxygenase-1(HO-1), NADH-quinone oxidoreductase-1(NQO-1), and glutamate cysteine ligase catalytic subunit (GCLC). These results suggested that HBO treatment triggered antioxidative effects against intestinal epithelial barrier dysfunction by promoting Nrf2 signaling pathway after SCI.

Objective:To investigate the factors affecting the prognosis of sudden sensorineural hearing loss (SSNHL), so as to guide the comprehensive treatment combined with hyperbaric oxygen (HBO) on SSNHL.Methods:A retrospective analysis was conducted on 112 patients (115 ears) with SSNHL who were admitted to the Department of Hyperbaric Medicine of Beijing Chao-yang Hospital from June 2018 to September 2021. They were divided into effective group (cured + markedly effective + effective) and ineffective group. The factors, i. e., head magnetic resonance angiography (MRA) and neck vascular ultrasound, were compared between the two groups, and then binary logistic regression analysis was performed.Results:There were 45 cases (46 ears) in the effective group, 67 cases (69 ears) in the ineffective group, and the effective rate was 40.0%. After two courses of HBO treatment, ultrasound-assisted examination was performed. Internal carotid artery plaques were found in 53 cases and vertebral artery plaques were found in 12 cases. Head MRI showed white matter lesions (WMLs) in 53 cases and MRA showed intracranial vascular stenosis in 26 cases. Types of hearing loss curves: 12 cases (12 ears) were hearing loss in low tone frequencies, 16 cases (16 ears) were hearing loss in high tone frequencies; 44 cases (45 ears) were hearing loss in all tone frequencies; and 40 cases (42 ears) were total deafness. Hearing loss degree: 22 cases (22 ears) were mild, 29 cases (30 ears) were moderate, 17 cases (17 ears) were severe, and 44 cases (46 ears) were extremely severe. The time from onset to first treatment, the type of hearing loss, and the degree of hearing loss between the two groups were significantly different ( P<0.01). Logistic regression analysis showed that the type of hearing loss and the time from onset to first treatment were correlated with the prognosis of SSNHL ( P<0.01). Conclusion:The prognosis of SSNHL with early HBO intervention was better, while the prognosis of SSNHL patients with intracranial vascular stenosis was poor. The SSNHL patients with abnormal head MRA results need more attention.