Soil salinity restricts sugar beet growth and productivity, creating a need for effective agronomic approaches to improve plant performance under salt-affected conditions. However, the comparative responses of sugar beet to different foliar-applied exogenous substances under controlled NaCl stress and field saline–alkali conditions remain insufficiently understood. In this study, sugar beet (Beta vulgaris L.) cultivar ‘HI0479’ was used to evaluate the effects of different concentrations of PAA@Mn3O4 nanoparticles (PMO), methyl jasmonate (MeJA), and γ-aminobutyric acid (GABA). A pot experiment was first conducted under controlled NaCl stress to identify concentrations for subsequent field evaluation, followed by a one-season field experiment under non-saline–alkali and saline–alkali soil conditions. Based on the overall responses of growth and physiological traits, 100 mg L−1 PMO, 100 mg L−1 MeJA, and 1000 mg L−1 GABA were selected for field evaluation. Under the imposed NaCl treatment, these foliar treatments improved growth and physiological performance and were associated with changes in photosynthetic characteristics, antioxidant enzyme activities, MDA content, osmolyte accumulation, and selected growth-related hormone levels. Under saline–alkali field conditions, PMO, MeJA, and GABA increased storage-root yield by 13.59%, 12.69%, and 12.37%, respectively, compared with the control. Although estimated storage-root sugar concentration decreased, the corresponding estimated sugar yields increased by 10.77%, 9.79%, and 7.41%, respectively. These results show that the three foliar treatments produced favorable growth- and yield-related responses under the experimental conditions evaluated while also revealing a trade-off between storage-root yield and estimated storage-root sugar concentration. The findings provide a basis for further agronomic evaluation of foliar PMO, MeJA, and GABA in sugar beet grown under salt-affected conditions.

IntroductionThe KUP/HAK/KT family is the largest group of potassium ion transporters in plants and plays a central role in K+ uptake, transport, and abiotic stress responses. However, the function of individual KUP genes in salt-tolerant crops remains under explored. In this study, BvKUP13 was isolated from the salt-tolerant beet variety AK3018.MethodsWe cloned and analyzed the sequence, subcellular localization, and expression profile of BvKUP13 under salt stress. Transgenic Arabidopsis thaliana plants overexpressing BvKUP13 were generated to evaluate its role in salt stress tolerance.ResultsBvKUP13 encodes a protein of 732 amino acids, shows striking sequence similarity to KUP proteins from Chenopodium quinoa and Spinacia oleracea, and is localized to the endoplasmic reticulum membrane. The gene is predominantly expressed in leaves and is upregulated by NaCl treatment. Overexpression in Arabidopsis significantly improved salt tolerance, as indicated by enhanced photosynthetic efficiency, maintained Na+/K+ balance, elevated antioxidant enzyme activity, and higher osmolyte levels. Reduced MDA and ROS accumulation further supported the protective role of BvKUP13 under salt stress.DiscussionThese findings demonstrate that BvKUP13 enhances salt tolerance by regulating ion homeostasis and strengthening physiological and biochemical stress responses. BvKUP13 is a potential candidate gene for engineering salt-tolerant sugar beet varieties.

The sugar beet (Beta vulgaris L.) industry in China occupies a pivotal position in the national sugar supply, yet drought in its major cultivation areas has become a key limiting factor for its high-quality development. Glycosyltransferases (GTs) play a pivotal role in plant responses to abiotic stress, particularly in the regulation of drought resistance. However, the systematic identification of the BvUGT90 gene family in sugar beet and the functional characterization of its members under drought stress remain largely unexplored. In this study, drought stress was simulated in the sugar beet cultivar ‘HI0466’ using the weighing method to regulate soil moisture. Samples were collected at different stress durations and after rewatering for subsequent experimental analyses. In this study, 121 members of the BvUGT90 family were identified in sugar beet, and a comprehensive analysis was conducted on their gene structures, phylogenetic relationships, promoter cis-acting elements and expression patterns under drought stress. The results showed that these 121 members were unevenly distributed across 9 chromosomes. The proteins they encode had an average amino acid length of 474, with molecular weights ranging from 10.78 to 99.10 kDa and theoretical isoelectric points (pI) from 4.68 to 8.69 (with an average of 5.76). Notably, 110 of these members (accounting for 90.91%) were identified as hydrophilic proteins. Synteny analysis indicated a high degree of homology between the BvUGT90 family members in sugar beet and their orthologous genes in Arabidopsis thaliana. Analysis of promoter cis-acting elements revealed the presence of six major categories of core elements in the promoter regions of BvUGT90 genes, including hormone-responsive elements, stress-responsive elements and pathway regulatory elements. Transcriptomic data showed that 45 BvUGT90 family members exhibited significant responsiveness to drought stress. Proteomic analysis demonstrated that 10 of these members were significantly upregulated at the protein level under drought stress, and these results were further validated by quantitative real-time polymerase chain reaction (qRT-PCR). Integrated transcriptomic and proteomic analyses identified Bv_005070_jjst.t1 and Bv6_140060_stjc.t1 as the family members with the most prominent responses to drought stress. Furthermore, transgenic transformation of sugar beet was performed, which confirmed that Bv_005070_jjst.t1 plays an important role in drought stress resistance. The findings of this study provide direct candidate genes from this family for drought-tolerant sugar beet breeding.

Non-specific lipid transfer proteins (nsLTPs) participate in lipid-related processes and plant responses to abiotic stress, yet their roles in drought tolerance in sugar beet remain largely unclear. In this study, a genome-wide analysis identified 15 nsLTP genes in sugar beet, distributed across six chromosomes, and promoter analysis revealed multiple cis-acting elements associated with abiotic stress responses. Among these genes, BvnsLTP10 exhibits sustained up-regulation in leaves and peak induction in roots and stems under PEG-induced drought. Subcellular localization analysis showed that BvnsLTP10 is a secreted protein localized to the cell wall/apoplast, suggesting a role in extracellular lipid transport. Functional characterization in transgenic Arabidopsis demonstrated that BvnsLTP10 overexpression enhanced drought tolerance, as evidenced by increased fresh weight (1.85-fold), relative water content (1.41-fold), chlorophyll content (+ 36

[Objective] NAC transcription factors play a significant role in plant resistance to abiotic stress. In this study, the NAC transcription factor gene BvNAC40 was cloned from the drought-resistant sugar beet variety ‘HI0466’, and its protein structure and expression under drought stress were analyzed. This research provided a theoretical basis for further analysis of BvNAC40 and the breeding of drought-resistant sugar beet varieties. [Methods] BvNAC40 gene was cloned using the PCR method, and its protein structure was analyzed by bioinformatics methods. Subcellular localization was observed with green fluorescent protein (GFP). Expression of BvNAC40 under drought stress was analyzed by qRT-PCR. Overexpression vectors and virus-induced gene silencing (VIGS) vectors for BvNAC40 were constructed using homologous recombination. [Results] The CDS of BvNAC40 was 1 182 bp long, encoding 393 amino acids, which was classified within the NAC transcription factor family, with a transmembrane domain at the C-terminus. Subcellular localization showed that it was primarily located in the nucleus. Under drought stress, BvNAC40 expression was upregulated in both the leaves and roots of sugar beets. [Conclusion] The BvNAC40 gene is specifically expressed in response to drought stress in sugar beets, laying the foundation for future studies using transgenic technology.

Sugar beet is one of the important sugar crops in China, and drought has become the main constraint on the high-quality development of the sugar beet industry due to the regional factors such as lower precipitation, uneven seasonal distribution and limited water resources and so on. Heat shock protein 70 (HSP70) plays an important role in plant growth, development and abiotic stress response. However, there is still very limited information about the identification of HSP70s and drought resistance in sugar beet. In this study, bioinformatics was used to explore the HSP70s in sugar beet, and their gene structure, phylogenetic relationship, chromosome mapping, cis element distribution and expression under drought stress were analyzed. The results showed that a total of 28 BvHSP70 genes were identified, with sequence length ranges from 1551 bp to 3240 bp, which were distributed on 9 chromosomes of sugar beet. The number of amino acids encoding proteins are 516 1079, and the theoretical isoelectric point is between 4.98 and 9.33, most of which are acidic hydrophobic proteins, which are mainly located in the endoplasmic reticulum and cytoplasm. Collinearity analysis showed higher homology with rice and maize, and analysis of gene structure and protein conserved motif showed that it had different degrees of differentiation during the evolution of sugar beet. Analysis of cis-elements in the promoters revealed their association with growth and development, hormone response, and abiotic stress. RNA-seq and proteomics analysis showed that 20 genes of BvHSP70s responded to drought stress, and qRT-PCR validation confirmed that 10 of these genes were upregulated, and they were specifically expressed in roots, petioles and leaves of sugar beet, and more genes were expressed in roots. The results of this study lay a foundation for the subsequent in-depth analysis of the gene function of the BvHSP70 family in sugar beet, and also provided a theoretical basis for further exploring the response mechanism of beet under drought stress.

Sugar beet (Beta vulgaris L.) is an important economic crop and a primary source of sugar in northern China, characterized by strong stress tolerance and high nutritional value. Microbial inoculants can promote crop growth by regulating soil enzyme activities, enriching dominant beneficial bacterial genera in rhizosphere soil, and improving the availability of soil nutrients. This study aimed to investigate the role of microbial inoculants in sugar beet production and their potential to replace chemical fertilizers and put forward the scientific hypothesis that microbial inoculants can increase soil nutrients and improve the soil microenvironment. A two-year field experiment was conducted: in 2022, treatments with different application rates of Bacillus subtilis and Trichoderma spp. inoculants were set up to screen the optimal inoculant and its dosage (M1); in 2023, based on this optimal inoculant (M1), treatments with reduced chemical fertilizer input were established to explore the mechanisms underlying the maintenance of sugar beet yield and quality. The results showed that the M1N2 (75 kg/ha fertilizer and 20% less nitrogen fertilizer) treatment significantly increased nitrogen, phosphorus, and potassium agronomic use efficiencies by 91.48%, 51.94%, and 53.50%, respectively, compared with the control (CK). Soil urease, catalase, and sucrase activities were significantly enhanced by 14.57%, 66.84%, and 222.46%, respectively. The treatment also significantly increased the relative abundance of beneficial bacterial genera such as JG30-KF-CM45 and KD4-96, while sugar beet yield was significantly increased by 5.53% relative to the CK. This study provides a theoretical basis for the application of microbial inoculants and the reduction in chemical fertilizers in sugar beet production.

Soil salinization is a significant factor that severely limits the production of high-quality sugar beet in China. However, little is known about the physiological and molecular regulatory mechanism of sugar beet in response to salt stress. In this study, salt-tolerant (AK3018) and salt-sensitive varieties (IM1162) were screened from 50 sugar beet cultivars, and transcriptome analysis identified 3281, 2614, 1930, and 4866 differentially expressed genes (DEGs) in the AK_L_C-VS-AK_L_S, AK_R_C-VS-AK_R_S, IM_L_C-VS-IM_L_S, and IM_R_C-VS-IM_R_S groups, respectively. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that DEGs responsive to salt stress were significantly enriched in multiple metabolic pathways, including proline and betalain biosynthesis, antioxidant enzyme activity, chlorophyll biosynthesis, and ion transmembrane transport. Additionally, the contents of proline, betaine, and soluble sugar and the activities of catalase and ascorbate peroxidase were significantly increased in sugar beet under salt stress. AK3018 had higher chlorophyll content, photosystem II activity, and more K+ and less Na+ in leaf than IM1162 under salt stress. These results indicate that sugar beet can accumulate osmoregulatory substances, maintain the reactive oxygen species balance, improve the photosynthetic system, and reconstruct ion homeostasis in response to salt stress. The results provide a deeper understanding of the physiological and molecular mechanisms of sugar beet in response to salt stress and provide a large number of candidate genes for molecular salt tolerance breeding in sugar beet.

NAC transcription factors form a plant-specific family essential for growth, development, and stress responses. NTLs, a subfamily of the NAC transcription factor family, belong to the membrane-bound transcription factors (MTFs). These proteins contain transmembrane domains that enable rapid nuclear translocation in response to environmental stimuli, thereby regulating target gene expression. As a major sugar crop, sugar beet is primarily cultivated in arid and semi-arid regions, where drought stress significantly impairs yield and quality, underscoring the urgent need to improve its drought tolerance. This study identified the NTL gene family in sugar beet and analyzed its gene structure, evolutionary relationships, cis-regulatory elements, drought-induced expression patterns, and BvNTL2’s role in drought resistance. The BvNTLs family comprises five members located on five distinct chromosomes. Their promoters harbor cis-regulatory elements related to ABA and drought stress, and their expression is drought-responsive. Under drought stress, BvNTL2 translocates to the nucleus, where its transmembrane domain is cleaved, resulting in its direct nuclear localization. Functional validation in Arabidopsis demonstrated that BvNTL2 overexpression enhances drought tolerance by increasing antioxidant enzyme activities and promoting the expression of ABA-related genes. This study highlights BvNTL2 as a promising candidate gene for the genetic improvement of drought-resistant sugar beet.

BRASSINAZOLE-RESISTANT (BZR) transcription factors, key elements of brassinolide (BR) signal transduction, play an important role in regulating plant growth and development. However, little is known about the molecular regulatory mechanism of BZR in sugar beet taproot growth. In this study, BvBZR1 expression was significantly induced by exogenous BR treatment. Transgenic sugar beet overexpressing BvBZR1 exhibited a higher taproot diameter compared with the wild type, mainly due to a significant enhancement in the spacing between cambial rings by increasing the size and layers of parenchyma cells. BvBZR1 regulated the expression of BvCESA6, BvXTH33, BvFAD3, and BvCEL1 and enhanced cell wall metabolism to promote sugar beet taproot growth in parenchyma cells and the development of each cambium ring. In addition, BvBZR1 overexpression significantly increased the accumulation of sucrose and soluble sugars in the taproot, which was attributed to its ability to regulate the expression of BvSPS and BvINV and improve the activity of BvSPS, BvSS-S, BvSS-C, and BvINV enzymes in each cambium ring and parenchyma cell in the sugar beet taproot. These results suggest that BvBZR1 can regulate the expression of genes related to cell wall and sucrose metabolism, improve corresponding enzyme activity, and promote the development of each cambium ring and parenchyma cell, thereby promoting the growth and development of sugar beet taproots.

In this study, members of the BvDof transcription factor family were identified in the beet genome data (Beta vulgaris L.) Through systematic analysis, 22 BvDof family genes were found in the beet genome, and they were divided into nine groups by phylogenetic analysis. Fifteen members of the BvERF family were involved in the transition to rapid root tuber growth. There was a tandem replication during the generation of the Dof gene family in sugar beet. Bv1_zfms, Bv_ofna, Bv5_racn, and Bv6_augo may be involved in the regulation of secondary cambium development in the beet root tuber. Bv9_nood, Bv1_zfms, and Bv6_cdca may be related to the growth rate of root tubers. The results provide a reference for further elucidating the molecular mechanism of the BvDof transcription factor, which regulates the development of beet root tubers.

Reaumuria trigyna, a wild and endangered salt-secreting small shrub, is distributed in arid and semi-arid areas of Inner Mongolia, China. An H+-pyrophosphatase gene (RtVP1) was isolated from R. trigyna according to transcriptomic data, which encoded a plasma membrane and tonoplast-localized protein. RtVP1 was quickly upregulated by NaCl and exogenous abscisic acid treatment and rescued the sucrose deficiency sensitive phenotype of the AtVP1 mutant (avp1). Transgenic Arabidopsis overexpressing RtVP1 exhibited a higher leaf area, plant height, fresh weight, root length, and soluble carbohydrate accumulation compared to the wild type (WT) under normal conditions. RtVP1 overexpression increased the seed germination rate and decreased the reduction rate of fresh weight, root length, and chlorophyll content in transgenic plants under salt stress. Catalase enzyme activity, proline content, relative water content, and soluble sugar content were significantly increased in transgenic Arabidopsis under salt stresses, but the malondialdehyde content was dramatically decreased. More K+ and less Na+ were accumulated in transgenic Arabidopsis leaves, resulting in a relatively lower Na+/K+ ratio. In transgenic Arabidopsis roots, K+ was unchanged, but Na+ and the Na+/K+ ratios were reduced compared to those in WT. More Na+ and K+ were accumulated in the intracellular of transgenic yeast, and the Na+/K+ ratio was significantly reduced compared to the control. These results showed that R. trigyna RtVP1 promotes the vegetative growth of plants, mainly by regulating carbohydrate metabolism, and confers salt tolerance in transgenic Arabidopsis by maintaining Na+/K+ homeostasis and enhancing the antioxidant and osmotic regulatory capacity. These results indicated that RtVP1 can serve as an important candidate gene for genetic improvement of crop yield and salt tolerance.

Sugar beet is an important sugar crop, and its roots are mainly used for processing raw materials to produce products such as sugar, molasses, and saccharin, as well as being used as fodder for livestock. BvCPD, a key enzyme gene for brassinosteroid (BR) synthesis, regulates the development of parenchyma cells and vascular bundles by promoting BR synthesis, which promotes the expansion of the sugar beet taproot and influences the growth, development, and yield of sugar beets. This study investigated the impact of BvCPD on the physiological metabolism of sugar beet utilizing BvCPD overexpression, silent, and wild-type (WT) lines. BvCPD increased the chlorophyll content and maximum photochemical efficiency and improved the photosynthetic characteristics of sugar beet leaves. Simultaneously, BvCPD increased the rate of sugar beet taproot respiration and ATP content by enhancing the activities of phosphoglycerate kinase, alcohol dehydrogenase, sucrose synthase, and sucrose synthase catabolism. Moreover, BvCPD induced changes in the sugar fraction content, which increased the sugar yield of a single plant. In addition, BvCPD promoted water absorption, nitrogen accumulation, and lignin/cellulose synthesis activities, facilitated by increased activities of phenylalanine ammonia-lyase, cinnamyl alcohol dehydrogenase, cellulose synthase, and protein serine/threonine phosphatases, providing the requisite energy and materials for sugar beet growth. These findings not only provide a new perspective for understanding the physiological mechanisms regulating the growth of sugar beets but also provide a theoretical basis for the future improvement of sugar beet varieties through molecular breeding techniques.

Sugar beet (Beta vulgaris L.) is a major sugar crop in China and an economic crop with regional advantages in the Inner Mongolia Autonomous Region. Currently, abiotic stresses are among the main factors restricting the high-quality development of the sugar beet industry. Recent research has shown that NAC transcription factors play an important role in regulating plant growth and development, as well as in resisting abiotic stress. To determine the specific expression of the NAC transcription factor family in different organs of sugar beet under different abiotic stress conditions, this study systematically analyzed the expression response of 52 members of the NAC transcription factor family in different organs to abiotic stress conditions using reverse transcription polymerase chain reaction technique. The responses of NAC transcription factor genes to different abiotic stresses were identified in sugar beet leaves and roots; 18 and 17 NAC transcription factor genes were found to respond specifically to abiotic stress in sugar beet leaves and roots, respectively. The research results provide a reference basis for using NAC transcription factors to develop stress-resistant germplasm resources in sugar beets.

Inner Mongolia, a major region in China for growing sugar beet, faces challenges caused by unscientific water and fertilizer management. This mismanagement restricts the improvement of sugar beet yield and quality and exacerbates water waste and environmental pollution. This study aims to evaluate the effects of reduced water and fertilizer on the growth and physiological metabolism of sugar beet taproot. Field experiments were conducted in Ulanqab, Inner Mongolia, in 2022 and 2023, using a split-plot design with three levels each of fertilization and irrigation. The study analyzed the effects of reduced water and fertilizer treatments on fresh taproot weight, respiration rate, energy metabolism, respiratory enzyme activity, and gene expression in sugar beet taproot. It was found that a 10% reduction in fertilizer significantly increased the beet taproot fresh weight. Further research revealed that during the rapid leaf growth phase and the taproot and sugar growth period, a 10% reduction in fertilizer upregulated HK and IDH gene expression and downregulated G6PDH gene expression in the beet taproot. This increased HK and IDH activities, decreased G6PDH activity, enhanced the activity of the EMP-TCA pathway, and inhibited the PPP. Taproot weight was positively correlated with the respiration rate, ATP content, EC, and ATPase, HK, and IDH activities, thereby increasing the taproot growth rate and taproot fresh weight, with an average increase of 4.0% over two years. These findings introduce a novel method for optimizing fertilizer use, particularly beneficial in water-scarce regions. Implementing this strategy could help farmers in western Inner Mongolia and similar areas improve crop yield and sustainability. This study offers new insights into resource-efficient agricultural practices, highlighting the importance of customized fertilization strategies tailored to local environmental conditions.

Sugar beet is a characteristic cash crop in Inner Mongolia, and water and fertilizer is an important management measure in sugar beet planting. The interaction between water and nitrogen is of practical significance in regulating the growth of sugar beet. The coordinated relationship between water and fertilizer can achieve the purpose of water saving, fertilizer saving, cost saving and efficiency increasing. The cultivation method of drip irrigation under plastic mulch was adopted in this study. The effects of water nitrogen supply on water metabolism and photosynthetic performance of sugar beet were studied through two years of field experiments in order to provide theoretical basis for improving the utilization efficiency of water and fertilizer and rational fertilization and irrigation. The results showed that the water nitrogen supply level and its proportion had influence with the water potential, Transpiration rate (Tr), Photosynthetic rate (Pn) and stomatal Conductance (Cond) of sugar beet leaves. And they were significantly positively correlated with the yield of sugar beet. When the water potential of sugar beet leaves was -0.38 similar to -0.23 MPa in the period of leaf fast growth, root and sugar growth, and -0.45 similar to -0.38 MPa in the period of sugar accumulation, the yield and sugar content were the highest. Under the coupling condition of water and nitrogen, the irrigation amount of sugar beet was 1350 similar to 1427 m(3)center dot ha(-1), and the nitrogen application amount was 150 similar to 179.22 kg center dot ha(-1). Sugar beet has the highest utilization rate of light energy and yield and sugar content.

Constitutive photomorpogenic dwarf (CPD) is a pivotal enzyme gene for brassinolide (BR) synthesis and plays an important role in plant growth, including increasing plant biomass and plant height, elongating cells, and promoting xylem differentiation. However, little is known about the function of the CPD gene in sugar beet. In the current study, we isolated CPD from Beta vulgaris L. (BvCPD), which encodes protein localized in the nucleus, cell membrane, and cell wall. BvCPD was strongly expressed in parenchyma cells and vascular bundles. The transgenic sugar beet overexpressing BvCPD exhibited larger diameter than that of the wild type (WT), which mainly owing to the increased number and size of parenchyma cells, the enlarged lumen and area of vessel in the xylem. Additionally, overexpression of BvCPD increased the synthesis of endogenous BR, causing changes in the content of endogenous auxin (IAA) and gibberellin (GA) and accumulation of cellulose and lignin in cambium 1-4 rings of the taproot. These results suggest that BvCPD can promote the biosynthesis of endogenous BR, improve cell wall components, promote the development of parenchyma cells and vascular bundle, thereby playing an important role in promoting the growth and development of sugar beet taproot.

Carrying out the teaching activities of the course Civics is an important way for colleges and universities to cultivate composite talents, how to build the Civics construction in a reasonable and effective way is a problem worth studying. This requires that in the usual teaching process, teachers of professional courses in agronomy have a good atmosphere with students, interact with students, and strengthen the quality of students' moral cultivation in the usual education. In order to enhance students' conduct and enable them to master the learning knowledge while also becoming well-rounded and qualified individuals of value. In today's continuous development of education, students' moral education is also needed to pay enough attention.

为探讨H+-焦磷酸酶编码基因对甜菜磷吸收和抗性的影响,实现优良基因在甜菜基因工程中的利用,研究在甜菜中超表达拟南芥液泡膜H+-焦磷酸酶编码基因AVP1,对转基因甜菜分析其耐低磷、耐盐性和抗旱性.结果显示,AVP1基因在甜菜植株的叶片和块根中表达,且在逆境胁迫下增强表达量响应胁迫;低磷处理条件下,转基因甜菜与野生型甜菜相比具有更高的含磷量,可提高甜菜对磷的吸收利用效率;干旱、盐胁迫处理条件下,AVP1基因在转基因甜菜中显著上升,在盐胁迫或干旱处理条件下,转基因植株的生长受抑程度相对较轻.随着盐和干旱胁迫的加剧,转基因植株体内MDA含量与野生型植株相比较低而脯氨酸含量显著增加,AVP1基因可通过减轻逆境对甜菜细胞膜的损伤及提高甜菜细胞的渗透调节能力,进而增强甜菜对高盐和干旱胁迫的抗性.

甜菜是我国北方地区重要的糖料作物。本研究以甜菜抗旱品种‘HI0466’叶片为材料,研究了其形态及生理变化对不同干旱胁迫处理的响应,采用i TRAQ技术进行了差异蛋白质组学分析。结果表明,在干旱胁迫过程中,随着土壤含水量的降低,甜菜幼苗萎蔫程度逐步加重,幼苗叶片气孔阻力明显增大,叶片ABA含量显著升高,复水后各指标均可恢复到接近对照水平,表明供试材料已在形态和生理上产生了响应。重度干旱胁迫处理与对照相比,共检测到差异表达蛋白163个,其中90个上调表达, 73个下调表达。部分上调表达差异蛋白可通过增强抗氧化能力、降低水分散失来增强甜菜对干旱胁迫的适应。