目的:探讨免疫组化在鉴别皮脂腺瘤和基底细胞癌(BCC)中的意义.方法:对31例皮脂腺瘤和31例结节型BCC患者标本分别行常规苏木精-伊红(HE)染色和免疫组化染色,并观察其结果.结果:免疫组化示31例皮脂腺瘤患者上皮抗原(EA)均为阴性;上皮膜抗原(EMA)在导管及成熟皮脂腺细胞中均呈阳性表达;24例雄激素受体(AR)呈阳性表达,余7例均为阴性;D2-40阳性表达28例,阴性3例.而结节型BCC患者中30例EA阳性,1例阴性;EMA阳性4例,阴性27例;AR阳性表达15例,阴性16例;D2-40 5例灶状阳性,余26例均阴性.结论:免疫组化示皮脂腺瘤患者EA阳性、EMA阴性和D2-40阳性,可较好地与结节型BCC相鉴别.

雄激素性秃发(androgenetic alopecia,AGA)又称脂溢性脱发,是最常见的慢性脱发性疾病,在男性患者中常表现为典型的男性型脱发,与遗传、雄激素水平及其受体等因素有关.由于毛发稀疏,头皮显露,影响美观,往往对患者心理和社交造成影响.本研究以信息调查表形式对男性AGA患者进行调查并进行皮肤镜检查,通过对数据进行整理和分析,进一步了解男性A-GA患者的人口学特征、流行病学特点、临床表现、皮肤镜特征、疾病相关因素及对患者的影响等.

Objective To measure histidine triad nucleotide?binding protein 1(HINT1)protein expression and gene promoter methylation, and to analyze the relationship between HINT1 gene promoter methylation and clinical pathological features of melanoma. Methods Fifty?six patients with melanoma and 51 patients with nevus were enrolled as subjects and controls, respectively. Methylation?specific PCR (MSP) was performed to measure the methylation of HINT1 gene promoter in lesional and paratumoral tissue specimens from the patients with melanoma, as well as in lesional specimens from the patients with nevus. Immunohistochemistry was carried out to quantify the expression of HINT1 protein in these tissue specimens. Results MSP showed that the methylation rate of HINT1 gene promoter was significantly higher in melanoma tissues than in paratumoral and nevus tissues(76.8%[43/56]vs. 33.9%[19/56]and 35.3%[18/51], χ2 = 20.810 and 18.749, respectively, both P < 0.05), but was insignificantly different between paratumoral and nevus tissues(χ2=0.022, P>0.05). Immunohistochemistry revealed that the expression rate of HINT1 was 21.4%(12/56)in melanoma tissues, compared to 82.4%(42/51)in nevus tissues(χ2 = 39.633, P <0.01). There was a significant difference in the methylation rate of HINT1 promoter between HINT1?positive and ?negative melanoma tissues(6/12 vs. 37/44[84.1%], P<0.05), and between Clark levelⅠ-ⅡandⅢ-Ⅴmelanoma tissues(59.1%[13/22]vs. 88.2%[30/34],χ2=6.365,P=0.012). Conclusions HINT1 protein is lowly expressed in melanoma, which may be associated with high methylation of its gene promoter. Moreover, the high methylation ofHINT1 gene promoter may be involved in the initiation and progression of melanoma.

目的 探讨雄激素受体(AR)在原发性乳房外Paget病组织中的表达及其与疾病侵袭性间的关系.方法 应用免疫组化方法,检测20例原位乳房外Paget病、18例侵袭性乳房外Paget病石蜡组织切片中AR的表达情况.结果 38例原发性乳房外Paget病中男26例,女12例,年龄(45～ 84)岁,平均年龄为64岁.AR在原发性乳房外Paget病中明显表达,AR高表达率为58％(22/38);其中,AR在侵袭性及原位乳房外Paget病中的高表达率分别为78％(14/18)和40％ (8/20),相比具有统计学意义(x2值为5.401,P值为0.020).结论 AR的异常表达与原发性乳房外Paget病的发生发展及侵袭性可能有关.

Objective To analyze clinicopathologic features of sebaceoma. Methods Clinical, pathologic and immunohistochemical findings from 31 cases of sebaceoma were retrospectively analyzed. The clinicopathologic features of sebaceoma were investigated. Results There were 9 males and 22 females. The patients′ age was 53.90 ± 15.40 years, and the clinical course was 9.41 ± 13.75 years. Sebaceoma predominantly affected the face. The common lesion of sebaceoma was red, yellowish?red, skin?colored or slight brown papules, with no subjective symptoms in most cases. Histopathologically, neoplasms had symmetric structures, and were located in the dermis. Epidermal involvements were found in 9 cases. The neoplasm cells were mainly composed of basaloid cells, a few mature sebocytes and some transition cells. The proportion of mature sebocyts was less than 1%in 26 cases, less than 20%in 2 cases, and 20%-40%in 3 cases. Mitoses were occasionally found in 5 cases. One patient was complicated by eccrine poroma. Varying amounts of ducts were found in all the patients. Immunohistochemical staining showed that epithelial membrane antigen was expressed on ducts and mature sebocytes in all the patients, while epithelial antigen was undetected in any of the patients. Carcinoembryonic antigen, androgen receptor and D2?40 were found in 20, 24 and 28 patients with sebaceoma, respectively. Conclusions The diagnosis of sebaceoma mainly depends on histopathological examination. Combined immunohistochemical detection of epithelial membrane antigen, androgen receptor and D2?40 is beneficial to its differential diagnosis.

临床常规免疫组化检测通常采用辣根过氧化酶显色系统(即DAB显色)，阳性反应呈棕黄色颗粒。但在黑素瘤组织中，免疫组化阳性的棕黄色颗粒常与组织原有黑素颗粒混淆，难以鉴别，若黑素颗粒较多，结果判断较困难。既往有文献报道，采用Giemsa染液进行后续复染能够很好的使组织内黑素颗粒显示为墨绿色，以区分DAB显色阳性的棕黄色颗粒[1]。本文采用甲苯胺蓝染料在苏木精复染的基础上进行双重复染，背景细胞清晰，黑素颗粒取得与Giemsa染液复染相似的结果，效果满意，现报道如下。

Objective To investigate the relationship of tumor-associated macrophage (TAM) infiltration in lesions with clinicopathologic features of cutaneous malignant melanoma (CMM) and epithelialmesenchymal transition (EMT)-related indicators.Methods Fifty-four CMM and 15 benign melanocytic nevus paraffin-embedded tissue samples were collected.An immunohistochemical study was performed to determine the expression pattern and intensity of the TAM marker CD163 as well as EMT indicators epithelial cadherin (E-cadherin),neurologic cadherin (N-cadherin) and vimentin in these tissue samples.Count data were expressed as mean ± standard deviation.Statistical analysis was carried out using t test,one-way analysis of variance (ANOVA) and multivariate linear regression analysis with the software SPSS 17.0.Results CD163-positive TAMs were detected in CMM samples,but not in benign melanocytic nevus samples.The mean TAM count per high power field in tissue samples was significantly different between invasive and in situ CMM (35.08 ± 13.78 vs.16.97 ± 8.74,P ＜ 0.05),and between melanoma with lymph node metastasis and that without (56.00 ± 3.07 vs.28.70 ± 18.52,P ＜ 0.05),and among melanoma with different Clark levels (P ＜ 0.05).Additionally,the mean TAM count increased with the increase in Clark level from Ⅰ to Ⅳ,but was insignificantly different between Clark level Ⅳ and Ⅴ (P ＞ 0.05).No significant difference was observed in TAM count between patients of different gender or age groups,between acral and non-acral melanoma,or between ulcerated and non-ulcerated melanoma (all P ≥ 0.05).Multivariate linear regression analysis showed that TAM count in CMM was associated with five factors:Clark level,ulceration,tumor progression,N-cadherin and vimentin.Conclusion TAMs may accelerate tumor progression and increase tumor malignancy by promoting EMT in CMM.

Objective To analyze the frequency and type of mutations in the c-kit gene,and to measure the expression of c-kit protein in patients with acral malignant melanoma.Methods Skin tissue specimens were collected from the lesions of 115 patients with cutaneous malignant melanoma (CMM) and 30 patients with acral melanocytic nevi,as well as normal skin of 15 healthy human controls.PCR and DNA sequencing were performed to analyze the sequence of the c-kit gene,and immunohistochemical staining was conducted to observe c-kit protein expression in tissue samples.Statistical analysis was done by Chi-square test,Mann-Whitney U test and Spearman's rank correlation test.Results C-kit protein was expressed in 90.3％ (84/93) of acral CMM specimens,81.8％ (18/22) of non-acral CMM specimens,and 63.3％ (19/30) of acral melanocytic nevus specimens.Among 58 acral invasive CMM specimens,29 showed strong expression of c-kit protein in tumor cells at the dermal-epidermal junction,8 showed negative or weak c kit expression in invasive and infiltrating tumor cells in the dermis.The expression rate of c-kit protein was significantly higher in acral CMM specimens than in acral melanocytic nevus specimens (x2 =12.14,P ＜ 0.05),and higher in acral than in non-acral invasive CMM specimens (94.8％ (55/58) vs.11/13,x2 =4.18,P ＜ 0.05).No significant correlation was observed between the elinicopathological feature of melanoma and expression of c-kit protein in in situ,invasive or metastatic acral CMM tissues (all P ＞ 0.05).C-kit gene mutations were detected in 4 out of the 115 CMM cases,including L576P mutation in 3 cases and K642E mutation in 1 case.All the 4 patients carrying mutations were diagnosed as acral CMM,and exhibited diffuse and strong expression of c-kit protein.Conclusions The expression of c-kit protein is stronger in acral than in non-acral invasive CMM lesions.All the mutations detected in these cases are common typesof c-kit mutations in acral CMM,but the mutation frequency is lower than that reported in foreign literature.

Objective To measure the expression of hypoxia-inducible factor (HIF)-1α in acral malignant melanoma (MM) tissue and to investigate its relationship with the stem cell factor (SCF)/c-kit pathway.Methods Immunohistochemical staining was performed to measure the expression of HIF-1α in tissue specimens from lesions of 93 patients with acral MM,21 with non-acral MM,39 with acral melanocytic nevi,and from the normal acral skin of 15 healthy human controls.Meanwhile,the expression of c-kit was detected by immunohistochemical staining in the 93 acral MM tissue specimens.Statistical comparisons were carried out by chi-square test and Mann-Whitney U test.The relationship of HIF-1α expression with c-kit expression as well as tumor progression and staging was assessed by Spearman correlation analysis.Results Immunohistochemistry showed that the expression rate of HIF-1α was 87.10％ (81/93) in acral MM specimens,90.48％ (19/21) in non-acral MM specimens,15.38％ (6/39) in acral melanocytic nevus specimens,but 0 (0/15) in the normal acral skin specimens.The expression of HIF-1α was significantly higher in acral MM lesions than in normal acral skin and acral melanocytic nevus lesions (both P ＜ 0.01),and significantly different between acral MM and non-acral MM lesions (P ＜ 0.01).Moreover,HIF-1α expression was positively correlated with Clark level and Breslow depth of melanoma (rs =0.442,0.368,respectively,both P ＜ 0.01),with the progression of acral MM (from in situ to aggressive and metastatic MM) (rs =0.420,P ＜ 0.01),and with the expression of c-kit (rs =0.307,P ＜ 0.01).Conclusions HIF-1α is highly expressed in acral MM,positively correlated with the staging,progression and aggression of MM,and co-expressed with c-kit in acral MM tissue,suggesting that both HIF-1α and c-kit take part in the pathogenesis of acral MM.

Objective To investigate the effect of the Notch-RBP-J signaling pathway on the phenotypic differentiation of RAW264.7 murine macrophage-like cells cocultured with B16F10 murine melanoma cells.Methods A small interference RNA (siRNA) targeting the CBF1/RBP-Jκ gene was designed.RAW264.7 murine macrophage-like cells were divided into four groups:unsilenced co-culture group cocultured with B16F10 cells,silenced co-culture group transfected with the designed siRNA and cocultured with B16F10 cells,blank control group cultured alone,positive control group induced by interleukin-4 (IL-4).After additional culture for different durations,Western blot,enzyme-linked immunosorbent assay (ELISA) and flow cytometry were conducted to determine the phenotype of macrophages,and reverse transcription (RT)-PCR was performed to detect the expressions of notch1,notch2,DLL1,DLL4 and Hes1 genes in macrophages.Statistical analysis was carried out by one-way analysis of variance (ANOVA),repeated measures ANOVA,linear trend test and the Bonferroni method with the SPSS17.0 software.Results Western blot showed that the relative expression level of CD163 in RAW264.7 cells was 1.016 ± 0.018 in the blank control group,1.274 ± 0.034,2.065 ± 0.094 and 3.615 ± 0.144 in the unsilenced co-culture group at 24,48 and 72 hours respectively,and 3.099 ± 0.071 in the positive control group,with significant differences between these groups (n =4,F =527.42,P ＜ 0.01).There was a significant increase in CD163 expression in RAW264.7 cells in the unsilenced co-culture group compared with the blank control group.As ELISA revealed,the levels of IL-10 in the culture supernatant of RAW264.7 cells were (167.61 ± 3.527),(433.433 ± 5.558) and (679.673 ± 8.101) ng/L in the unsilenced co-culture group at 24,48,and 72 hours respectively,significantly higher than those in the silenced co-culture group ((63.403 ± 0.856),(103.427 ± 2.072),(202.297 ± 3.61) ng/L,respectively,F =8.01,P ＜ 0.05).Western blot and flow cytometry both demonstrated a statistical reduction in the expressions of CD163 and CD206 in RAW264.7 cells in the silenced co-culture group compared with the unsilenced co-culture group (both P ＜ 0.05).The mRNA expressions of notch signaling pathwayrelated genes in RAW264.7 cells were also attenuated in the silenced co-culture group in comparison with the unsilenced co-culture group.Conclusions Although most of the RAW264.7 cells in the silenced co-culture group exhibited the M2 phenotype,their polarization toward M2 phenotype was weakened compared with those in the unsilenced co-culture group,implying that the activation of the Notch-RBP-J signaling pathway promotes the M2-polarization of RAW264.7 cells.

The relationship of ovostatin 2 (OVOS2) expression with the clinicopathological features of cutaneous malignant melanoma (CMM) was investigated to identify OVOS2 expression in cutaneous melanocytic lesions, and to reveal whether OVOS2 has a function in melanoma progression. Eight specimens of CMM and paracancerous tissue were analyzed using real‐time polymerase chain reaction (PCR) and western blot for the mRNA and protein expression of OVOS2, respectively. Immunohistochemical staining was performed on 52 CMM and 62 nevi, followed by clinicopathological significance analysis. The proliferative cells were visualized by staining with Ki‐67 antibody. The intensity of angiogenesis was assessed by staining with vascular endothelial growth factor (VEGF). Real‐time PCR and western blot analyses showed that OVOS2 was significantly upregulated in cutaneous melanoma than in paired normal skins. Immunohistochemistry showed that 86.5% (45/52) of malignant cases showed OVOS2 cytoplasmic expression compared with 29% (18/62) in benign nevi. OVOS2 expression was significantly higher in invasive and metastatic melanoma than in in situ melanoma (P < 0.01). Furthermore, OVOS2 expression was positively correlated with the known prognostic variables of melanoma including clinical stage, Clark level and Breslow depth. It was also significantly associated with ulcer status, Ki‐67 labeling index and VEGF expression in primary melanoma. OVOS2 expression was significantly increased in CMM, which increased incrementally from benign nevi to melanoma and appeared to be involved in the progression of melanoma.

Objective To detect the expression of tissue inhibitor of metalloproteinase-4 (TIMP-4) in cutaneous malignant melanoma (CMM) tissue and to assess its relationship with melanoma proliferation,invasion and metastasis.Methods Western blot was conducted to measure the protein expression of TIMP-4 in five fresh lesional and paratumoral tissue specimens of CMM and three fresh tissue specimens of nevi.Immunohistochemistry was carried out to quantify the expression of TIMP-4,Ki-67,matrix metalloproteinase-2 (MMP-2),vascular endothelial growth factor (VEGF) and CD63 in paraffin-embedded tissue samples from 43 cases of CMM and 51 cases of nevi.The degree of malignancy of melanoma was evaluated in these lesions.Results Western blot analysis showed that the expression of TIMP-4 was significantly higher in 4 of 5 CMM tissue specimens than in corresponding paratumoral tissue specimens and nevus tissue specimens.Immunohistochemistry revealed that the expression rate of TIMP-4 was 86.04％ (37/43) in melanoma tissue,compared to 19.6％ (10/51) in nevus tissue (x2 =31.55,P ＜ 0.05).The expression of TIMP-4 increased sequentially from in situ melanoma to invasive and metastatic melanoma (rs =0.309,P ＜ 0.05).As far as CMM was concerned,the TIMP-4 expression was uncorrelated with any of the known prognostic variables including clinical stage,Clark level,Breslow depth,presence of ulcer,and Ki-67 expression (all P ＞ 0.05),but positively correlated with the expressions of VEGF (rs =0.345,P ＜ 0.05) and CD63 (rs =0.555,P ＜ 0.01).The median expression level of TIMP-4 was significantly higher in MMP-2-positive than in MMP-2-negative melanoma tissue samples (3 vs.0,P ＜ 0.01).Conclusions TIMP-4 protein is highly expressed in CMM tissue,which may be closely associated with the initiation and progression of CMM,especially with the metastasis of and angiogenesis in CMM.

A 59-year-old man presented with a 3-month history of infiltrated aching nodules and plaques all over the body.Physical examination revealed multiple firm,erythematous nodules and plaques with marked tenderness on the trunk and limbs.Histopathological examination showed that there was a diffuse infiltrate of unique lymphoid cells with dark-stained nuclei and dust-like chromatin in the dermis and subdermis.Immunophenotype analysis showed that the tumor cells were diffusely positive for CD20,CD79a,CD10,terminal deoxynucleotidyl transferase (TdT),PAX5,and tor Ki-67 (70％).Bone marrow examination revealed an active proliferation of bone marrow cells,which were predominated by lymphocytes,with lymphoblasts and prolymphocytes accounting for 66％.The patient was diagnosed with cutaneous precursor B-cell lymphoblastic lymphoma/leukemia.

Objective To investigate the expressions of Na+/H+ exchanger regulatory factor 1 (NHERF1) and β-catenin in extramammary Paget's disease tissue as well as their significance.Methods Immunohistochemistry was performed to detect the protein expressions of NHERF1 and β-catenin in paraffin-embeded tissue samples from 18 patients with in situ and 22 patients with invasive extramammary Paget's disease.Results There was a high expression of NHERF1 protein in 18 (81.82％) invasive and 7 (38.89％) in situ extramammary Paget's disease samples (x2 =7.78,P ＜ 0.01).Statistical differences were observed in the membrane expression rate and cytoplasmic or nuclear expression rate of β-catenin between invasive and in situ extramammary Paget's disease tissue samples (0 (0/22) vs.33.33％ (6/18),x2 =8.63,P ＜ 0.01; 81.82％ (18/22) vs.44.44％ (8/18),x2 =6.08,P ＜ 0.05).In extramammary Paget's disease in situ tissue samples,the expression of NHERF1 was negatively correlated with the cytomembrane expression of β-catenin (ρ =-0.488,P ＜ 0.01),but positively correlated with the cytoplasmic or nuclear expression of β-catenin (ρ =0.623,P ＜ 0.01),and there was a negative correlation between the cytomembrane and cytoplasmic or nuclear expression of β-catenin (ρ =-0.572,P ＜ 0.01).Conclusions There is an abnormal expression of NHERF1 and β-catenin in extramammary Paget's disease tissue,which may be associated with the initiation,progression,and invasion of primary extramammary Paget's disease.

Objective: To study the expression of the acetylcholine receptor in different types of pemphigus patients, and investigate the relationship between the expression and disease activity of different types of pemphigus. Methods: Immunohistochemistry and ELISA were used. Results: In a total of 30 patients, the OD value of AChR IgG in peripheral blood was positive in 8 patients, among them 7 cases were pemphigus vulgaris patients and 1 pemphigus foliaceus. There was no statistical difference (P > 0.05) compared with normal controls. In the AChR-positive patients, the disease was more severe. The OD value of AChR IgG in peripheral blood declined after the disease was under control. In pemphigus vulgaris, subtype AChRM3 distributed in the basal layer as brown granules with deep color and larger particles with different sizes. The expression of AChRM3 in pemphigus foliaceus showed no significant difference compared with that of normal controls. In pemphigus vulgaris and pemphigus foliaceus, subunit AChRα was distributed in the whole layer as brown granules with deep color and larger particles with different sizes. Conclusion: In pemphigus vulgaris and pemphigus foliaceus, AChR antibody expression levels are different. AChR antibody is more likely to appear in the peripheral blood of Dsg3-positive patients. In the skin biopsy of Dsg3-positive patients, the staining patterns of both AChRα protein and AChRM3 protein have changed. This shows that the Dsg3 antibodies are more likely to lead to abnormal AChR pathway.

目的:检测p53和Gadd45α蛋白在皮肤鳞状细胞癌和基底细胞癌组织中的表达.方法:应用免疫组化法对30例皮肤鳞状细胞癌和25例基底细胞癌组织中的p53和Gadd45α蛋白表达进行检测.结果:p53蛋白在皮肤鳞状细胞癌和基底细胞癌组织中的表达阳性率分别为56.67%、48%,与正常皮肤组织比较差异均有显著性(P=0.006;0.025);Gadd45α在皮肤鳞状细胞癌和基底细胞癌中表达分别为43.33%、52%,均高于正常皮肤组织的表达(P=0.031;0.010).高分化皮肤鳞状细胞癌组阳性表达率为75%,高于中低分化组的22.22%(P=0.008).结论:皮肤鳞状细胞癌和基底细胞癌组织中p53、Gadd45α异常表达可能参与了发病过程.

Objective To investigate the expression profiles of three sex hormone receptors including androgen receptor (AR),estrogen receptor (ER) and progesterone receptor (PR) in primary extramammary Paget's disease (EMPD) and their significance.Methods Immunohistochemical methods were used to examine the expressions of androgen receptor,estrogen receptor and progesterone receptor in formalinfixed paraffin-embedded tissue samples from 30 patients with EMPD.Results Androgen receptor was observed in 19 (63.3％) of the 30 tissue specimens,18 (72％) of the 25 specimens from male patients,and 1 out of the 5 specimens from female patients.Neither estrogen receptor nor progesterone receptor was detected in any of these specimens.Conclusions EMPD is hormone-dependent.Androgens and their receptors may participate in the initiation and progression of primary EMPD.

目的:检测皮肤鳞状细胞癌组织中E-钙黏素和α-SMA的表达.方法:用免疫组化法检测30例皮肤鳞状细胞癌组织中E-钙黏素和α-SMA蛋白的表达.结果:与癌旁及正常皮肤组织相比,E-钙黏素在皮肤鳞状细胞癌中表达减弱或缺失(P<0.05),中低分化与高分化癌组织组间比较差异有显著性(P<0.05);皮肤鳞状细胞癌癌巢周边组织中α-SMA阳性表达,中低分化与高分化癌组织组间比较差异也有显著性(P<0.05).结论:皮肤鳞状细胞癌发生上皮-间质转化,肿瘤相关成纤维细胞可能参与了其转化和侵袭过程.

Objective To investigate the effects of HINT1 on the growth of and apoptosis in malignant melanoma in nude mouse models established by subcutaneous xenotransplantation of human melanoma A375 cells. Methods Three groups of nude mice were subcutaneously inoculated with A375 cells transfected with pcDNA 3.1/myc-His (-) A-HINT1 (HINT1-A375), A375 cells transfected with pcDNA 3.1/myc-His (-) A empty vector (neo-A375), and untransfected A375 cells, respectively. Then, the growth of transplanted tumors was observed and tumor formation rate was calculated. Thirty-three days after the inoculation, mice were killed and tumor tissue was obtained followed by the examination of tumor weight and volume. Histopathology was performed to observe the morphological features of tumor cells and in situ end labeling technique (TUNEL)was carried out to assess the apoptosis in transplanted tumor cells. Results The tumor formation rate was consistently 100% in the three groups. The transplanted tumor in HINT1 group grew more slowly than that in the other two groups, and significant difference was observed as early as day 18 (P ＜ 0.01 ). Lower tumor weight and volume were noted in the HINT1 group compared with the neo group and A375 cell group (0.04 ±0.00 g vs. 0.23 ± 0.00 g and 0.29 ± 0.03 g, 0.06 ± 0.04 cm3 vs. 0.34 ± 0.15 cm3 and 0.43 ± 0.19 cm3 respectively, all P ＜ 0.01 ). Histopathology revealed smaller tumor nests, slight atypia of tumor cells with no obvious pathologic mitoses or necrosis in HINT1 group in comparison with the other two groups. Immunohistochemistry and TUNEL revealed that the percentage of apoptotic cells in HINT1 group was statistically higher than that in the neo group and A375 cell group (12.87% ± 1.18% vs. 3.22% ± 0.49% and 3.00% ± 0.53%, both P ＜0.01 ). Conclusion High expression of HINTl could inhibit the growth of and promote the apoptosis in malignant melanoma in nude mice subcutaneous xenotransplantation models, suggesting that HINT1 gene might be responsible for tumor suppression in human malignant melanoma.