家庭中饲养猫咪,会在一定程度上限制它的运动,有些疾病随之而来.猫下泌尿道综合征就是一个高发的困扰主人和兽医师的疾病之一.下面介绍一例该综合征的治疗,以期为临床诊断与治疗提供参考. 1 基本情况 初诊时间2018年5月21日,品种:暹罗猫,性别:雄性已绝育.年龄:2岁10个月.体重:4.1kg.主诉:经常蹲猫砂,排尿姿势还叫,但猫砂里没有结块.

造成莱姆病的病原体为伯氏疏螺旋体,是一种在全球范围内对人类和动物健康存在严重危害的蜱传疾病,可引起人体多系统、器官的损害.该病的流行特点呈现出明显的季节性,到目前为止,全球伯氏疏螺旋体至少可分为20个不同的基因型,其中我国北方以伽氏(B.garinii)和阿氏(B.afzelii)为主.

蜱是吸血性的体表寄生虫,主要寄生在马、牛、羊等家畜和野生动物体表,是多种病毒、细菌、寄生虫的传播媒介.立克次氏体为专性细胞内寄生微生物,蜱为其保存宿主,可经卵垂直传播,保持其种群连续性.立克次氏体病是在全球范围内对人类和动物健康造成严重威胁的自然疫源性疾病,四季均可发生,已经引起广大研究者的重视.目前在我国的黑龙江、吉林、内蒙古等十三个省份和地区都存在斑点热立克次氏体的自然疫源地.

奶牛乳房炎是奶牛乳腺在化学、物理或微生物等因素刺激下引起的一种炎症反应,给乳品行业带来了巨大的经济损失,影响了奶牛养殖业的发展, 造成这一局面的原因是引起感染的病原微生物在世界范围内存在广泛,并且发病率比较高.

幼犬蜂窝织炎又称作幼犬腺疫或者幼犬脓皮病.该病发病原因复杂,可能与免疫系统异常、遗传因素、皮肤屏障受到损坏、各种变态反应及内分泌失调等有关.此病高发于3周龄到6月龄的任何品种的幼犬.目前诊断方法主要为病变部位细胞学检查及皮肤组织病理学检查.

为获得猪附红细胞体吉林感染株2-DE图谱,并筛选猪附红细胞体吉林感染株免疫原性蛋白,本试验采用2-D电泳分析猪附红细胞体蛋白表达谱,利用感染血清与二维电泳凝胶进行免疫印迹.结果表明:二维电泳共检测到(60±5)个蛋白质点,蛋白主要集中在pH值6～9和分子量45～25 ku.二维电泳中的9个蛋白点被猪附红细胞体感染血清所识别.本研究将为进一步开展猪附红细胞体病的疫苗和诊断候选分子研究奠定基础.

本实验建立了羊新孢子虫间接荧光抗体试验方法(IFAT),并采用该方法检测了310份来自吉林省部分地区的成年羊血清.结果检出羊新孢子虫抗体阳性血清30份,阳性率为9.68%.结果表明吉林省某些地区存在羊新孢子虫的感染.

对延吉市某养殖场送检的病料,通过细菌的涂片检查、分离培养、生化培养、菌型鉴定与动物实验的方法;涂片镜检可见两端钝圆,短粗的革兰氏阳性杆菌.厌氧条件下分离培养,可见凸起,边缘整齐,圆形菌落.在含铁牛乳培养基中呈爆烈发酵现象,综合上述特点,结合临床诊断,确定为魏氏梭菌病.为确定致死动物的菌型,进一步做毒素中和试验,定型为A型魏氏梭菌.

The H9N2 avian influenza virus (AIV) has become increasingly concerning due to its role in severe economic losses in the poultry industry. Transmission of AIV to mammals, including pigs and humans, has accelerated efforts to devise preventive strategies. To develop an effective oral vaccine against H9N2 AIV, a recombinant Lactobacillus plantarum NC8 strain expressing the hemagglutinin (HA) gene of H9N2 AIV was constructed in this study. Mice were orally immunized with the recombinant NC8-pSIP409-HA strain, and sIgA, IgG and HI antibodies were produced by the NC8-pSIP409-HA strain, which also induced CD8þ T cell immune responses. Most importantly, oral administration produced complete protection against challenge with mouse-adapted H9N2 virus. These results indicate that the recombinant NC8pSIP409-HA was more effective at inducing the mucosal, humoral and cellular immune responses. Therefore, L. plantarum NC8-pSIP409-HA could become a promising oral vaccine candidate against

仔猪腹泻是规模化养猪场所面临的重要防治疾病之一,据统计,在规模化猪场中仔猪的死亡率约占猪只死亡总数的60％～70％,而腹泻引起的死亡数达20％[1].可见,有效防治仔猪的腹泻病对猪场来讲有多重要,然而要想做好仔猪腹泻的预防及治疗,就要对该病有足够的了解,目前仔猪腹泻的原因较复杂,往往是混合感染,治疗起来较困难,给猪场造成损失,下面我们就来分析一下各种致病原因及防治措施.

布鲁菌病(brucellosis)是由布鲁菌引起的人兽共患传染病,家畜牛、羊、猪最常发生,且可传染给人和其他家畜,其特征是生殖器官和胎膜发炎,引起流产、不育和各种组织的局部病灶.本病广泛分布于世界各地,目前在我国人畜间仍有发生,给畜牧业和人类的健康带来严重危害[1].

为了构建携带报告基因增强型绿色荧光蛋白(EGFP)基因的反转录病毒载体,并观察EGFP基因在SP2/0小鼠骨髓瘤细胞中的表达情况.试验将增强型绿色免疫荧光基因片段定向插入反转录病毒载体pFB-neo,获得重组反转录病毒质粒pFB-NE,利用三质粒共转染系统,将含有目的基因的重组反转录病毒质粒pFB-NE以及含有辅助病毒gag-pol和env基因的质粒pVPack-GP、pVPack-10A1共转染HEK-293细胞,用产生的重组反转录病毒感染小鼠骨髓瘤细胞SP2/0,并在荧光显微镜下观察EGFP基因的表达.结果表明:重组反转录病毒栽体pFB-NE构建正确,重组病毒包装成功,EGFP基因在细胞中能够稳定表达.

To construct a retroviral vector mediated mammalian cell expression cell line of the human immunodeficiency virus(HIV-1) core protein,the recombinant retroviral vector pFB-gag-EGFP was constructed by inserting core protein gene gag of HIV-1 and enhanced green fluorescent protein gene EGFP into pFB-neo by turns.The recombinant retroviral vector,the pVPack-GP plasmid containing the helper virus gag-pol gene and pVPack-10A1 plasmid containing env gene were cotransfected into packaging cell line HEK-293T with liposome-mediated transduction,and then the recombinant retrovirus was collected and infected SP2/0 cells.The green fluorescent protein was observed under fluorescence microscope,and the expression of core protein Gag of HIV-1 was verified.The resistance cells were screened by G418.The core protein Gag of HIV-1 and green fluorescent protein could express in SP2/0 cells,and the mammalian cell line of the core protein gene gag of HIV-1 had been constructed successfully,which laid a mouse cell model of activity assay of anti-AIDS genetic engineering therapeutic agents and targeted drugs.

In this research,E.suis antigen which was purified and protected as envelope antigen was made.The optimal reaction conditions were determined and the kit was assembled initially.The result showed that the optimal concentration of antigen was 30 mg/L;the optimal concentration of serum dilution was 1∶100;the optimal condition of HRP-labeled rabbit anti-swine IgG was 1∶4 000;the optimal reaction condition of TMB was room temperature for 15 minutes.D450≥0.13 was positive,D450≤0.11 was negative,and 0.11-0.13 was suspected.The method has no cross-reaction with swine E.coli,swine fever and swine toxoplasmosis serum.Coefficient of variation of negative and positive serum at D450 was not exceed 10% after detecting for different 8 times.This showed that the kit had a good repeatability.The kits could be kept for 6 months by the determination of stability.60 samples of serum in Yanbian area were detected by the kit which had saved for 6 months.Compared with indirect ELISA,the result showed the the positive coincidence was 100%.This study provides a new effective way for diagnosis of eperythrozoonosis.

Goatpox, caused by goatpox virus (GTPV), is an acute feverish and contagious disease in goats often associated with high morbidity and high mortality. To resolve potential safety risks and vaccination side effects of existing live attenuated goatpox vaccine (AV41), two Semliki forest virus (SFV) replicon-based bicistronic expression DNA vaccines (pCSm-AAL and pCSm-BAA) which encode GTPV structural proteins corresponding to the Vaccinia virus proteins A27, L1, A33, and B5, respectively, were constructed. Then, theirs ability to induce humoral and cellular response in mice and goats, and protect goats against virulent virus challenge were evaluated. The results showed that, vaccination with pCSm-AAL and pCSm-BAA in combination could elicit strong humoral and cellular responses in mice and goats, provide partial protection against viral challenge in goats, and reduce disease symptoms. Additionally, priming vaccination with the above-mentioned DNA vaccines could significantly reduce the goats' side reactions from boosting vaccinations with current live vaccine (AV41), which include skin lesions at the inoculation site and fevers. Data obtained in this study could not only facilitate improvement of the current goatpox vaccination strategy, but also provide valuable guidance to suitable candidates for evaluation and development of orthopoxvirus vaccines.

Designing adjuvants that can induce strong cytotoxic T cell responses is largely required for preparing DNA vaccines. In this study we explored dual costimulatory molecules 4-1BBL and OX40L as adjuvants to improve the efficiency of the HIV multiple-epitope DNA vaccine. When explored in the human dendritic cell-T cell based coculture system,dual costimulatory molecules significantly enhanced the anti-HIV T cell response of the HIV mul-tiple-epitope DNA vaccine,as detected by intracellular cytokine staining to HIV antigens,cytokines accumulation in the cultures,and antigen-specific cytotoxic T lymphocyte responses. These results suggest that dual costimulatory molecules 4-1BBL and OX40L can effectively increase the potential of the HIV multiple-epitope antigen DNA vaccine and may provide an exciting approach for HIV therapy.

objective To develop a multiplex RT-PCR method for determination of foot-and-mouth disease virus(FMDV)type Asia 1 of XJ/03 and JS/05 topotypes.Methods The sequences of a large quantity of epidemic FMDV strains reported in GenBank were compared,based on which the primers for differential diagnosis of infection with FMDV type Asia 1 of XJ/03 and JS/05 topotypes by multiplex RT-PCR were designed,and the conditions for single and multiplex RT-PCR were optimized.The developed method was verified for specificity and used for determination of relevant viruses.Results Both the optimized single and multiplex RT-PCR methods showed high specificities.The determination results of 9 FMDV samples by the developed method were completely consistent with those by gene sequencing.Conclusion A multiplex RT-PCR method for determination of FMDV type Asia 1 of XJ/03 and JS/05 topotypes was developed.which laid a foundation of diagnosis and epidemiological investigation of FMDV as well as application of FMD vaccine.

Objective:The eukaryotic expression plasmid pVAXⅠ-P1-2A containing capsid protein precursor P1-2A gene of Asia-ⅠFMDV was constructed. Cellular and humoral immunity level of the mice immunized against pVAXⅠ-P1-2A was evaluated. Methods:The gene of FMDV P1-2A was amplified by RT-PCR, inserted into vector pMD18-T, then subcloned to eukaryotic expression vector pVAXⅠ. The eukaryotic expression plasmid pVAXⅠ-P1-2A transfected into the Hela cell, and detected by IFA. The mice were immunized against pVAXⅠ-P1-2A, the FMDV specific antibody was detected by ELISA, T lymphocyte proliferation analyzed by MTT, IFN-γ was detected by ELISPOT method. Results:The result of restriction endonuclease digestion was accordance with the anticipated objective strap size. IFA showed that there are flavovirens fluorescence in cells transfected with pVAXⅠ-P1-2A, and confirmed the expression of P1-2A gene in HeLa cell. The indirect ELISA test manifested that the antibody level was higher remarkably than the control groups (P0.05) in the 14th day after immunity, and more T lymphocyte and the cells that produce IFN-γ in immunized mice than the control groups (P0.05).The result showed that there are significant cellular and humoral immunity response in the mice immunized with pVAXⅠ-P1-2A. Conclusion: Eukaryotic expression plasmid pVAXⅠ-P1-2A was successfully constructed, and pVAXⅠ-P1-2A could induce specific humoral and cellullar immunity response in mice.

Objective To develop a purification procedure suitable for large-scale production of therapeutic plasmid DNA. Methods The culture of recombinant E. coli JM109 transformed with plasmid pVAXI-MEG-p24-4-1BBL-OX40L were harvested by hollow fiber column chromatography, cleaved with alkaline, then concentrated by hollow fiber column chromatography again and purified by molecular sieve, affinity and ion exchange chromatography. Perform overall control tests on the purified plasmid DNA by gel electrophoresis, PCR, nucleic protein calculator, BCA protein assay kit and endotoxin assay kit. Results The purified supercoiled plasmid DNA contained 91.2% of total plasmid and reached a purity(A260 / A280) of 1.8. The endotoxin content was less than 10 EU/mg. Little residual protein and no residual genome DNA were detected. All the quality indexes of purified plasmid DNA met the relevant requirements. Conclusion The developed purification procedure was time- and labor-saving, effective and suitable for the large-scale production of therapeutic plasmid DNA.