OBJECTIVE:To explore the role of hsa_circ_0095812 (circLRRC4C) in periodontitis and its mechanism with miR-485-3p and Thrombospondin-1 (THBS1). METHODS:Periodontal tissues were collected from periodontitis patients. Periodontal Ligament Cells (PDLCs) were stimulated with Lipopolysaccharide (LPS) and transfected. Cell viability, inflammation, apoptosis, and pyroptosis were analyzed. A mouse model of periodontitis was constructed and injected with a lentiviral plasmid vector targeting circLRRC4C. Immunohistochemistry was performed on the periodontal tissue of model mice. The relevant expression level of genes was measured via real-time reverse transcriptase-polymerase chain reaction or Western blot. The relationship between circLRRC4C and THBS1 with miR-485-3p was analyzed. RESULTS:CircLRRC4C was highly expressed in periodontitis tissues of patients and LPS-treated PDLCs. Downregulating circLRRC4C attenuated LPS-induced PDLC inflammation, apoptosis and pyroptosis and recovered cellular viability. CircLRRC4C acted as a sponge for miR-485-3p. CircLRRC4C affected LPS-induced PDLC apoptosis, pyroptosis and inflammation by regulating miR-485-3p. THBS1 was the target gene of miR-485-3p. Inhibition of THBS1 effectively improved LPS-induced periodontitis. CircLRRC4C aggravated LPS-induced PDLC apoptosis, pyroptosis and inflammation by regulating the miR-485-3p/THBS1 axis. Suppressing circLRRC4C effectively improved periodontitis in mice. CONCLUSION:CircLRRC4C induces periodontitis progression by adsorbing miR-485-3p-mediated THBS1 expression.

Purpose:Phototherapy have gained significant traction in the treatment of tumors. However, the successful implementation of these therapies relies on photosensitizers with superior properties and precise guidance mechanisms. Methods:In this study, we introduce an innovative method for the surface modification of titanium dioxide (TiO2) nanoparticles through HRP-catalyzed covalent incorporation of Mn(II) chelate (Mn-Dopa) and dopamine. Results:This modification extends TiO2 nanoparticels' light absorption from ultraviolet to the near-infrared (NIR) range, endowing the nanoparticles with MRI-guided phototherapy capabilities. The resulting nanotheranostics system, TiO2@PDA-MnDopa, demonstrated over 5-fold enhanced relaxivity compared to the monomeric MnDopa and exhibited synergistic phototherapy effects upon 808 nm laser excitation, with a photothermal conversion efficiency of 15.91%. In vitro and in vivo pharmacodynamics studies showed that the TiO2@PDA-MnDopa demonstrated good safety in the HSC3 cell line and corresponding tumor-bearing mice, while effectively inhibiting tumor growth under 808 nm laser excitation. Conclusion:This multifunctional nanotheranostic, integrating high relaxivity with synergistic PTT/PDT for MR imaging-guided phototherapy, holds great potential for applications in the early diagnosis, noninvasive treatment, and prognostic evaluation of oral squamous cell carcinoma.

目的 通过比较改良型FRⅢ与"2×4"矫治技术治疗替牙期前牙反患者的软硬组织变化,评价两种矫治方法的治疗效果,为临床选择应用提供理论参考.方法 选择2018年9月—2022年10月在本院口腔科门诊治疗的替牙期前牙反患者30例作为研究对象,根据治疗方法不同分为观察组(改良型FRⅢ)和对照组("2×4"矫治技术)两组,每组各15例.对两组病例矫治前后的软硬组织位置变化进行对比分析.结果 治疗完成后,观察组SNA、ANB、MP-FH、Y轴角、U1-SN、U1-NA、鼻唇角、颏唇角、面型角、上唇E线距、ULP增加(P＜0.05),SNB、Z角、NP-FH、L1-MP、U1-L1、下唇E线距减小(P＜0.05);对照组SNA、ANB、MP-FH、U1-SN、U1-NA、Y轴角、鼻唇角、面型角、上唇E线距增加(P＜0.05),SNB、NP-FH、U1-L1、下唇E线距、Z角减小(P＜0.05);两组之间,SNB、ANB、MP-FH、鼻唇角、颏唇角、面型角及Z角的变化量比较具有明显差异(P＜0.05).结论 两种技术治疗均取得良好的治疗效果,前牙反得到有效解除,咬合关系及面型恢复正常,具有临床应用价值,值得推广.改良型FRⅢ更好地改善软组织侧貌和面型,而"2×4"矫治技术用时更短,牙列关系得到更好的解决.

Augmentation of the alveolar bone is important before oral implantation. For large bone defects, it becomes necessary to apply guided bone regeneration (GBR) materials, accompanied by filling defect sites with autologous or allogeneic bone, or bone substitutes such as acellular bone powder. In this study, we tested a granular bone substitute and GBR membrane combination therapy in treating MC3T3-E1 and L929 cells in vitro and rat calvarial and alveolar defects in vivo. The recovery conditions of bone defects were monitored by micro-CT, and 3D reconstruction of the CT images was applied to evaluate the bone augmentation semi-quantitatively. Test GBR materials could support the proliferation of MC3T3-E1 cells, poly (p-dioxanone-co-L-phenylalanine) (PDPA)-based membrane could induce apoptosis of L929 cells. Among GBR membranes applied groups, the regeneration condition of defected calvarial defects of PDPA based membrane applied group was the best and this may be caused by its excellent positive space acquiring effect. However, in a complex bacteriogenic environment, the oral bone regeneration-guided efficacy of the PDPA membrane decreased in the post-repair stage with the aggravation of infections. By contrast, the antimicrobial membrane combined with the PDPA membrane exhibited continually increasing GBR efficacy at the later stage of repair owing to its multifunctional properties, which are infection-inhibiting and positive space acquiring. Therefore, multifunctional GBR membranes are preferable for GBR in complex oral environments, and further research should be conducted to determine their efficacy in other models.

目的 了解巴中市农村老年人群口腔卫生习惯及就医行为,为当地的口腔预防服务提供指导资料,为卫生部门进行口腔医疗卫生资源分配提供依据.方法 参照世界卫生组织《口腔调查方法》和全国口腔流行病学调查方法制作调查问卷,采用SPSS 26.0软件进行统计分析.结果 巴中市农村老年居民50.2％每天刷牙2次或2次以上,13.5％采用正确的刷牙方法,60.0％使用含氟牙膏,59.7％有饭后漱口习惯,10.2％牙刷使用时间不超过3个月,15.1％每次刷牙时间大于或等于3min.人均有口腔疾病,就医率为37.3％,14.3％选择市(县)级综合医院,48.9％选择乡镇医院口腔科或乡镇口腔诊所,36.7％选择游医;未就诊居民中35.1％家庭经济困难,29.5％对口腔健康认知错误,29.1％认为交通不便利,自认没有时间和无人陪同的居民分别占3.3％和3.1％.结论 巴中市农村老年人群口腔卫生习惯较差,就医率低,需加强卫生教育,改善卫生资源分配.

牙列缺损是一类常见的口腔疾病,种植技术是修复牙列缺损的一种重要方式.其中,种植位点骨量不足会导致植入种植体时出现植体暴露、初期稳定性不足等后果,进而影响种植修复疗效.引导骨再生(guided bone regeneration,GBR)是利用屏障膜进行骨增量的一种修复技术.其良好的屏蔽作用,可快速诱导骨形成,有效促进骨再生,扩大种植修复的适应范围.屏障膜是GBR疗效的主要决定因素,理想的屏障膜应具有良好的生物相容性、足够的机械强度(以维持空间及增加可操作性)、优良的细胞封闭功能(以防止上皮细胞迁入)、可生物降解且无严重免疫反应,并能进行主动诱导等特性.迄今为止,尚无一种理想的屏障膜能够持续提供完美的骨再生效果.因此越来越多的学者在膜的改性、复合膜的应用、功能梯度膜等方面进行了研究,致力于理想屏障膜的研发.本文将从屏障膜的分类、在骨增量中的应用、研究进展等方面进行综述,以期为研发理想的屏障膜提供一定的参考.

To obtain multifunctional materials suitable for guiding alveolar bone regeneration under infectious conditions, we prepared asymmetric membranes comprising space acquiring layer that involves fibroblast inhibitor poly(p-dioxanone-co-L-phenylalanine) (PDPA), an isolating dense layer that forms barrier between two layers and an osteogenesis inducing electrospinning layer which involves hydroxyapatite or hydroxyapatite & minocycline. Then the composition, crystallization, morphology, and hydrophilicity of asymmetric membranes were analyzed. Minocycline incorporated membranes controlled the expansion of Porphyromonas gingivalis (P. gingivalis) in vitro. Hydroxyapatite-incorporated asymmetric membranes promoted the expression of osteogenesis related genes RUNX2, OPN, ALP of MC3T3-E1 cells in vitro. The mineralization of MC3T3-E1 cells cultured with hydroxyapatite-incorporated asymmetric membranes were also promoted in vitro. Asymmetric membranes especially hydroxyapatite-incorporated ones guided the regeneration of the mandibular bone defect in vivo. Bone regeneration guided under infectious conditions was evaluated in a P. gingivalis infected alveolar bone defect model. Specifically, space acquiring layer containing asymmetric membranes effectively controlled connective tissue hyperplasia at defect sites. The excellent guided bone regeneration achieved by applying a single space acquiring layer membrane further indicates the importance of acquiring space actively to induce bone regeneration. Hydroxyapatite-minocycline incorporated symmetric membranes could simultaneously suppress alveolar bone reabsorption caused by infection and guide regeneration of defects. Therefore, the hydroxyapatite-minocycline incorporated asymmetric membrane may be more suitable to be applied in guiding regeneration of bone defects under complex infectious conditions.

Mn( ii )-metallostar structures prepared by coordination-driven self-assembly for theranostic applications: high relaxivities and synergistic PTT/PDT effects.

Children with obstructive sleep apnea（OSA） have received extensive attention from many scholars due to its high prevalence and serious complications. The diversity of etiology of upper airway obstruction determines the personalized clinical manifestations and treatment of different OSA children, and different specialists have different ideas and treatment methods, so there is no unified and detailed treatment plan. This review reviewed the latest research progress on risk factors, clinical symptoms and signs, early screening and diagnosis, intervention and treatment methods of OSA in children, in order to provide some reference for clinical screening and treatment of children suspected of OSA in orthodontics department.

随着口腔医学的发展,越来越多的口腔分支学科在临床诊疗中需要考虑上颌窦分嵴的解剖学因素,其位置、走向、高度等直接影响着方案制定及治疗效果,锥形束CT能够提供大量无创的、精确的数据利于研究上颌窦分嵴.本文就应用锥形束CT测量上颌窦分嵴解剖结构的研究进行综述.

The anatomy of the oral and maxillofacial sites is complex, and bone defects caused by trauma, tumors, and inflammation in these zones are extremely difficult to repair. Among the most effective and reliable methods to attain osteogenesis, the guided bone regeneration (GBR) technique is extensively applied in defective oral and maxillofacial GBR. Furthermore, endowing biofunctions is crucial for GBR materials applied in repairing defective alveolar and maxillofacial bones. In this review, recent advances in designing and fabricating GBR materials applied in oral and maxillofacial sites are classified and discussed according to their biofunctions, including maintaining space for bone growth; facilitating the adhesion, migration, and proliferation of osteoblasts; facilitating the migration and differentiation of progenitor cells; promoting vascularization; providing immunoregulation to induce osteogenesis; suppressing infection; and effectively mimicking natural tissues using graded biomimetic materials. In addition, new processing strategies (e.g., 3D printing) and new design concepts (e.g., developing bone mimetic extracellular matrix niches and preparing scaffolds to suppress connective tissue to actively acquire space for bone regeneration), are particularly worthy of further study. In the future, GBR materials with richer biological functions are expected to be developed based on an in-depth understanding of the mechanism of bone-GBR-material interactions.

随着采用正畸治疗纠正牙颌面畸形的人数日渐增多,治疗方案多样且个性化,加之生物-心理-社会医学模式兴起,使越来越多的人关注社会心理因素对正畸结果的影响,因此对正畸治疗临床质量的把控尤为重要.本文将从功能与美学、社会心理2个方面对正畸治疗效果评价标准的研究现状进行综述.

OBJECTIVES:This study aimed to prepare functional composite electrospinning fibrous membranes with the functions of antifibrosis and bacteriostasis, as well as to explore its repair effect on rat jaw defect. METHODS:Poly-(lactic acid-co-glycolic acid) fibrous membrane loaded with cyclic-arginine-glycine-aspartic acid sequence, ornidazole, and nanohydroxyapatite (n-HA) was prepared by electrospinning as the functional layer (GBRL) for adhering onto defective bone. A barrier layer with the function of supporting and isolating different functional layers was prepared by tape-casting method. Poly(p-dioxanone-co-l-phenylalanine) fiber membrane with the function of inhibiting fibrosis was prepared by electrospinning technology as the antifibrosis layer (AFL). The morphology of the composite membrane was characterized by scanning electron microscopy. The effects of different functional layers on the proliferation of mouse osteoblast precursor cells (MC3T3-E1) and mouse fibroblasts (L929) were studied by cell-proliferation test (CCK-8 method). The inhibitory effect of composite membrane on the proliferation of Porphyromonas gingivalis was studied by bacteriostatic circle test. A rat mandibular-bone defect model was established to study the repair effect of composite fiber membrane on bone-defect tissue. This repair effect was compared with that of collagen oral-repair membrane commonly used in clinics. RESULTS:The GBRL layer could support the proliferation of MC3T3-E1 cells, and the AFL layer could inhibit the proliferation of L929 cells. Composite membranes loaded with ornidazole could inhibit the proliferation of Porphyromonas gingivalis. Various composite membranes can induce the reconstruction of rat jaw defects, among which the composite membranes loaded with ornidazole and n-HA had the best repair effect, which was better than that of collagen oral-repair membrane. CONCLUSIONS:The electrospun membrane loaded with ornidazole and n-HA as the composite fiber membrane of GBRL layer had excellent antibacterial and bone-tissue-regeneration activity. The effect was better than that of the commonly used collagen oral-repair membrane. Therefore, this material has great potential to induce bone regeneration for defects caused by periodontal diseases.

目的 探究虚拟仿真技术的进阶式模式在口腔实验教学中的应用效果.方法 选取2019年6月—2020年6月川北医学院在读的口腔医学系学生作为对照组,选取2021年6月—2022年6月川北医学院在读的口腔医学系学生作为研究组,各39名.对照组采用传统教学法,研究组采用虚拟仿真技术的进阶式模式教学法.对比教学后两组口腔医学系学生操作考试成绩、自我能力提高情况、教学满意程度.结果 教学结束后,研究组操作考试成绩高于对照组,差异有统计学意义(P<0.05);研究组自我能力问卷得分高于对照组,差异有统计学意义(P<0.05);研究组教学满意度评分高于对照组,差异有统计学意义(P<0.05).结论 虚拟仿真技术的进阶式模式教学法可显著提升口腔医学系学生的学习效果,能够提高其操作成绩,利于促进学生自我能力提升,且学生对此教学方式的满意度较高.

Periodontitis is a chronic inflammatory disease that leads to the loss of alveolar bone, among several studies focusing on reconstructing periodontal bone caused by periodontitis, guided bone regeneration (GBR) is a promising approach. In this study a serial clinically applied antibiotics loaded poly(L-lactide-co-glycolide)/poly(L-lactide-co-ε-caprolactone) (PLGA/PLCA) fibrous mesh to prevent and reconstruct defective bone in periodontitis were prepared by electrospinning. Incorporation of antibiotics promoted the hydrophilicity but decreased the crystallinity of PLGA/PLCA membranes. Antibiotics could be sustained released from membranes. Metronidazole, minocycline, and doxycycline incorporated membranes could suppress Porphyromonas gingivalis (P. gingivalis) within 21 days in vitro. Metronidazole and minocycline incorporated membranes decreased 41% and 55.5% colony counts in rat gingival crevicular fluid in vivo. Minocycline-loaded membrane could support the proliferation of MC3T3-E1 cells and maintained 79% viability of human ligament fibroblasts cultured on it. And MC3T3-E1 cells could undergo osteoblastic differentiation when cultured with pure PLGA/PLCA membrane and minocycline incorporated membrane. Then in vivo repairable effects of those antibiotics loaded membranes were evaluated in alveolar bone defected P. gingivalis infected model. The application of minocycline loaded membranes, effectively prevented the bone resorption of periodontitis caused by P. gingivalis. After been treated with minocycline incorporated membrane, volume of defected bone of maintained at about 50% level of control rats. 8 weeks post-operation, newly regenerated bone was observed in the operative alveolar bone of the pure PLGA/PLCA membrane, metronidazole and minocycline incorporated PLGA/PLCA membrane treated groups. Minocycline/PLGA/PLCA electrospinning membrane is a promising GBR material that can be applied to guide regeneration of periodontitis-induced alveolar bone damage.

正畸治疗过程中,任何施加于矫治牙的力,必然同时产生一个方向相反、大小相同的反作用力,能抵抗该反作用力的结构被称为"支抗".上颌前突是临床常见的错颌畸形,该类错颌畸形患者常采用拔牙矫治进行正畸治疗,为获得正常的覆、覆盖,使侧貌更加美观,常需增强支抗以便在生物力学范围内最大程度内收前牙.根据支抗牙移动占据拔牙间隙的多少又将支抗分为强支抗(不超过拔牙间隙的1/3),中支抗(不超过拔牙间隙的1/2)和弱支抗(不超过拔牙间隙的2/3).临床常用牙齿自身作为支抗,但Nabbout 等[1]使用3D扫描仪计算出精确度更高的各个牙齿的支抗系数,表明牙齿所能提供的支抗较使用平片计算牙周膜面积所得出的支抗系数更低.因此在上颌前突治疗中,使用牙齿自身作为支抗被认为是危险的,必须增加辅助装置.

牙髓干细胞(dental pulp stem cells,DPSCs)是一种神经嵴来源的成体干细胞,具有良好的自我更新及多向分化特性,可向多种口腔组织相关细胞分化,且兼具免疫原性低等优势,因此作为种子细胞在组织工程及再生医学领域广泛应用.微小RNA(microRNA,miRNA)是一类通过作用于基因转录后RNA而调控靶基因表达的短链非编码RNA,在细胞生长、增殖、胚胎发育、组织分化和凋亡等多种生命活动中发挥重要调节作用.本文对DPSCs分化过程中微小RNA调节作用及其机制进行综述.

目的 分析改良型FR-Ⅲ功能矫治器矫正替牙期前牙反的临床疗效.方法 将上、下颌前牙可退缩至切对切关系作为选择标准,选取2017年6月至2019年12月川北医学院第二附属医院收治的利用改良型FR-Ⅲ功能矫治器矫正替牙期前牙反患者38例,其中男16例,女22例;年龄6.6～10.5岁;矫治时间4～11个月,平均(6.4±1.5)个月.对患者矫正前后模型及头侧片进行描绘定点上齿槽座点、下齿槽座点、第一磨牙上下近中牙尖、前鼻棘、上下切牙切缘点等,测量对比矫正前后各数值变化,评价临床矫治效果.结果 38例患者矫正后上颌中切牙点至前陆地平面角度变大[(103.2±3.5)°至(108.6±4.0)°],下切牙点至下颌平面的角度变小[(89.0±4.9)°至(85.5±3.1)°];ANB角增大[(-2.1±1.9)°至(-1.7±0.3)°];前鼻棘增大,水平向距增大[(68.3±3.5)mm至(71.5±3.2)mm],垂直向距更大[(46.2±3.2)mm至(48.8±2.9)mm],差异均有统计学意义(P<0.05).结论 改良型FR-Ⅲ矫治器较法兰克传统矫正器能快速、高效地矫治替牙期功能性前牙反.

目的:检测不同浓度TNF-α对牙周膜成纤维细胞ephrinB2/EphB4表达量的影响.方法:收集因正畸需要拔除的健康牙齿,培养原代牙周膜成纤维细胞;用不同浓度TNF-α分别刺激24 h,48 h后检测牙周膜细胞eph-rinB2/EphB4 mRNA及蛋白表达量.结果:24 h组ephrinB2与EphB4 mRNA浓度均降低,差异有统计学意义(P<0.05);48 h低浓度组对ephrinB2 mRNA表达量无影响,0.1μg/L使EphB4 mRNA表达量增加,高浓度组使ephrinB2/EphB4 mRNA表达量下降;24 h组,TNF-α浓度为0.1～1μg/L时,ephrinB2/EphB4蛋白表达量无明显变化,TNF-α浓度为10～1000μg/L时ephrinB2/EphB4蛋白表达量下降;48 h组,0.1μg/L TNF-α作用后使EphB4蛋白表达量上升,ephrinB2蛋白表达量无明显变化,1μg/L作用后,ephrinB2/EphB4蛋白表达量均无明显变化,10～1000μg/L刺激后ephrinB2/EphB4蛋白表达量均下降.结论:24 h时TNF-α使ephrinB2/EphB4 mRNA与蛋白表达量均降低,但在48 h时,低浓度作用下EphB4表达量上升,是否可以为低浓度炎症状态下骨改建的研究提供参考.

Erythropoietin-producing hepatomocellular receptors and their receptor-interacting proteins (Eph/ephrin) can participate in the regulation of growth and development and promote the development of diseases through short-distance signal transduction between cells. To study the mechanism of Eph/ephrin and oral-related diseases, we provided a new theoretical basis and a strategy for the treatment of oral diseases. The Eph/ephrin pathway has been used to regulate oral diseases, especially in periodontal disease prevention, orthodontic bone reconstruction, and biological treatment of oral tumors. This paper reviews the research progress of Eph/ephrin pathway in oral-related diseases.