Discipline and teaching team construction is the core work to university development.Chinese universities have made great achievements since reform and opening-up,but there are also some questions of how can them positive interaction with and harmonious development,this is a problem and a project in our universities at present.Beginning of their relations,the article focuses on the contradictions and conflicts example from pathophysiology subject of university of south China,search for ways to solve,try to achieve both harmonious interaction between them and improve the quality of teaching and school level constantly.

Mipu1 (myocardial ischemic preconditioning upregulated protein 1) is a novel N-terminal Kruppel-associated box (KRAB)/C2H2 zinc finger superfamily protein, that displays a powerful effect in protecting H9c2 cells from oxidative stress-induced cell apoptosis. The present study aims to investigate the effect of Mipu1 overexpression on oxidized low-density lipoprotein (oxLDL)-induced foam cell formation, cell apoptosis, and its possible mechanisms. New Zealand healthy rabbits were used to establish atherosclerosis model, and serum levels of triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were detected by an automatic biochemical analyzer. Sudan IV staining was used to detect atherosclerotic lesions. The RAW264.7 macrophage cell line was selected as the experimental material. Oil red O staining, high-performance liquid chromatography, and Dil-labeled lipoprotein were used to detect cholesterol accumulation qualitatively and quantitatively, respectively. Flow cytometry was used to determine cell apoptosis. Real-time quantitative polymerase chain reaction (PCR) was used to detect the mRNA expression of the main proteins that are associated with the transport of cholesterol, such as ABCA1, ABCG1, SR-BI, and CD36. Western blot analysis was used to detect the protein expression of Mipu1. There were atherosclerotic lesions in the high-fat diet group with Sudan IV staining. High-fat diet decreased Mipu1 expression and increased CD36 expression significantly at the 10th week compared with standard-diet rabbits. Mipu1 overexpression decreased oxLDL-induced cholesterol accumulation, oxLDL uptake, cell apoptosis, and cleaved caspase-3. Mipu1 overexpression inhibited the oxLDL-induced CD36 mRNA and protein expression, but it did not significantly inhibit the mRNA expression of ABCA1, ABCG1, and SR-BI. Mipu1 overexpression inhibits oxLDL-induced foam cell formation and cell apoptosis. Mipu1 overexpression reduces the lipid intake of macrophages and might be associated with the downregulation of CD36 expression in the presence of oxLDL.

Endothelial system is a dynamic balance system to maintain the normal flow of blood,which is very important to maintain local homeostasis.It has been confirmed that endothelial cell integrity of the structure and function are important to the normal vessel wall permeability barrier,immune defense,anticoagulant,antithrombotic and anti-inflammatory response.Microvascular endothelial cell damage is closely related to myocardial no-reflow,which can significantly reduce the survival rate of patients with no-reflow,being clinical treatment challenges.Studies have shown ischemia-reperfusion results in structure and function of endothelial cell injury,which promote the development of no-reflow.The corresponding clinical intervention measures in a certain extent can repair the damage of endothelial cells and improve myocardial no-reflow to a certain extent.The latest endothelial cell injurious mechanism and clinical treatment application are reviewed here,in order to provide new theoretical basis of prevention of myocardial no-reflow for clinical treatment.

The paper discusses discussion-based teaching in pathophysiology class for clinical medicine specialty.It includes three parts: self-study,discussion and summary.The self-study process combined with typical cases enables students to learn "how to learn," and the following discussion process enables students to learn "how to learn better," and finally the teacher makes a summary that will foster students' ability of "how to draw inferences about other cases from one instance." This type of teaching can not only help cultivate students′ individual learning method and improve their comprehensive quality,but also further improve teachers' teaching ability.

目的:探讨学生到医院收集病例并进行病例讨论教学对大二医学生临床思维能力培养的作用.方法:将病理生理学科研兴趣小组成员(共10人)分成三组分别到医院不同临床科室收集病例,随后各组将收集到的病例整理成幻灯片,最后老师和学生一起进行学习讨论和总结.结果:该教学方法激发了学生的学习兴趣和临床思维能力,培养了学生利用知识解决问题的能力.

Exploring the new preclinical medicine education model through constructing a special subject learning websit about the principle,prevention and cure of diseases around the standard of national medical education.It provides an Internet-based and bran-new learning platform for the research of the pathogenesis,prevention and cure of diseases for special-subject study and cooperation.Taking the advantage of the Internet,the new model encourages students to systematically use the knowledge and information from the website to solve problems and acquire self-study skills.Exploiting website as a net-based information technology tool,the new model enables users to reconstruct their knowledge and study in an innovative way.

Objective To investigate the role of anti-TL1A-Ab in the prevention of high glucose-induced ROS generation and ap-optosis in human umbilical vein endothelial cells(HUVEC).Method HUVEC were divided into six groups:normal control group,5.6 mM glucose plus TL1A group,high glucose control group,high glucose plus SOD plus CAT group,high glucose plus anti-TL1A-Ab group and high osmotic control group.ROS generation was determined by flow cytometry,apoptosis rate of HUVEC was determined by flow cytometry with Annexin V/PI double staining.Results Exposure of HUVEC to 22.4 mM glocose for 24 h resulted in a significant increase in ROS generation and apoptosis,compared with normal control group(P<0.01).HUVEC ROS formation and apoptosis rate significantly increased after adding exogenous TL1A to media containing 5.6 mM of glucose for 24 h,compared with normal control group and high glucose control group(P<0.01).However,high glucose-induced HUVEC ROS generation and apoptosis was significantly attenuated by 9μg/ml anti-TL1A-Ab [(50.63±4.05) vs(71.63±6.61),(5.07±0.47)%vs(23.70±3.20)%,P<0.01].Conclusion Anti-TL1A-Ab preventets high glucose-induced HUVEC apoptosis through ROS inhibition.

TL1A, a novel member of the TNF-superfamily, is an endothelial cell-specific inhibitor of angiogenesis, and a naturally existing modulator of vascular homeostasis through binding to DR3 and DcR3/TR6. In combination with IL-12 and IL23, TL1A mediates a strong costimulation of Th1 and Th17 cells, respectively, and is involved in immune responses. Especially, TL1A is expressed or highly expressed during inflammation, and induces the expression of many proinflammatory cytokines, such as IFN-γ, IL-8, IL-13, TNF-α, MPC-1, ICAM-1, VCAM-1, etc.. Therefore TL1A plays an important role in the development of inflammation diseases.

TNF-like ligand 1 aberrance(TL1A),a novel member of the TNF-superfamily,provides costimulatory signals to activated T lymphocytes through binding itself to its receptor—DR3 or DcR3.TL1A has some physiological activities such as inducing apoptosis and increasing secretion of Th cytokines.And it may play an important role in the activation of lymphocytes,the regulation of inflammatory response and the development of inflammatory bowel disease and atherosclerosis.

Objective To observe the influence of DPI on apoptosis and NOX4 mRNA expression in hUVECs stimulated by AngⅡ. Methods Morphology of hUVECs was observed by inverted microscope.hUVECs were divided into three groups:control group(treated without AngⅡ for 16 hours),AngⅡ(treated with 10-7 mol/L AngⅡ for 16 hours) and DPI intervention group(treated with 10-7 mol/L AngⅡplus 10μmol/l DPI for 16 hours).mRNA expression of NOX4 was detected by RT-PCR.Cell apoptosis was observed by Hoechest staining. Results The results showed that apoptosis of hUVECs in AngⅡ group was more than that in DPI intervention group.mRNA expression of NOX4 was increased in hUVECs treated with AngⅡ(P<0.05,vs control group).After treated with DPI,mRNA expression of NOX4 in hUVECs decreased(P>0.05,vs control group,P<0.05,vs AngⅡgroup). Conclusion DPI inhibits apoptosis and NADPH oxidase 4 expression of hUVECs induced by AngⅡ.

OBJECTIVE:To explore the influence of oxidized high-density lipoprotein (oxHDL) on the maturation and migration of bone marrow-derived dendritic cells (BMDCs) from C57BL/6J mice. METHODS:The C57BL/6J mice bone marrow cell suspension was prepared and purified. Recombinant granulocyte-macrophage colony-stimulating factor (rmGM-CSF) and recombinant interleukin-4 (rmIL-4) were used to promote monocytes to differentiate and suppress lymphocytes. Then 50 microg/mL oxHDL was added to stimulate BMDCs, using 50 microg/mL high-density lipoprotein (HDL) as homologous protein control, PBS as negative control, and 1 microg/mL lipopolysaccharide (LPS) as positive control. The CD86 and MHCII expression rates were detected with fluorescence-activated cell sorting (FACS). Liquid scintillation counting (LSC) was used in mixed lymphocyte reactions (MLRs) to reflect the ability of BMDCs in stimulating the proliferation of homologous T cells. Levels of cytokines IL-12 and IL-10 were detected by ELISA. The cell migration was evaluated with the transwell system. RESULTS:Compared with PBS group, the expressions of CD86 and MHCII, counts per minute of MLRs, secretion of IL-12 and IL-10, and number of migrated cells in oxHDL group and LPS group significantly increased (all P<0.05), while the increment was less in oxHDL group than LPS group. The number of migrated cells in oxHDL group was about twice of that in HDL group. CONCLUSION:OxHDL may promote the maturation and migration of BMDCs in vitro.

Cell Biology InternationalVolume 32, Issue 3 p. S6-S6 Epitope analysis of a novel Homo spains synapse associated protein, preparation of its antibody and protein expression study Ren Wang, Ren Wang Institute of Cardiovascular Disease, Nanhua University, Hengyang, 421001, ChinaSearch for more papers by this authorXiang Dong Yang, Xiang Dong Yang Institute of Cardiovascular Disease, Nanhua University, Hengyang, 421001, ChinaSearch for more papers by this authorDa Ming Feng, Da Ming Feng Institute of Cardiovascular Disease, Nanhua University, Hengyang, 421001, ChinaSearch for more papers by this authorWen Qing Sun, Wen Qing Sun Institute of Cardiovascular Disease, Nanhua University, Hengyang, 421001, ChinaSearch for more papers by this author Ren Wang, Ren Wang Institute of Cardiovascular Disease, Nanhua University, Hengyang, 421001, ChinaSearch for more papers by this authorXiang Dong Yang, Xiang Dong Yang Institute of Cardiovascular Disease, Nanhua University, Hengyang, 421001, ChinaSearch for more papers by this authorDa Ming Feng, Da Ming Feng Institute of Cardiovascular Disease, Nanhua University, Hengyang, 421001, ChinaSearch for more papers by this authorWen Qing Sun, Wen Qing Sun Institute of Cardiovascular Disease, Nanhua University, Hengyang, 421001, ChinaSearch for more papers by this author First published: 02 January 2013 https://doi.org/10.1016/j.cellbi.2008.01.032AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume32, Issue3March 2008Pages S6-S6 RelatedInformation

Objective To investigate the change of NOX4 mRNA expression in HUVECs stimulated by high concentration of glucose.Methods Morphology of HUVECs was observed by inverted microscope,expression of factor Ⅷ related antigen in HUVECs was investigated by immunohistochemistry.HUVECs were divided into three groups:control group(treated with physiological concentration of glucose for 16 hours),high glucose group(treated with 30 mmol/L glucose for 16 hours) and DPI intervention group(treated with 30 mmol/L glucose plus 10μmol/l DPI for 16 hours). mRNA expression of NOX4 was detected by RT-PCR.Results The results showed that there was expression of factor Ⅷ related antigen in HUVECs.mRNA expression of NOX4 was increased in HUVECs stimulation with high glucose(P<0.05,vs control group).After treated with DPI,mRNA expression of NOX4 in HUVECs decreased(P>0.05,vs control group).Conclusions The mRNA expression of NOX4 in HUVECs increased significantly stimulated by high glucose,while DPI could decrease NOX4 mRNA expression in HUVECs

AIM: In order to investigate the change of CD36 expression in atherosclerosis. METHODS: Chinese minipigs were fed a normal control diet (CD) or a high fat/high cholesterol diet (HFHC) for 12 months after common carotid artery injury induced by balloon denudation. Plasma total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and triglycerides (TG) were determined by commercially enzymatic methods. CD36 mRNA and protein levels were determined by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry, respectively. RESULTS: After HFHC for 12 months, plasma total cholesterol, HDL cholesterol and triglyceride in HFHC minipigs were increased compared with the control. CD36 expression and aorta PPARγ in HFHC minipigs were upregulated. CONCLUSION: HFHC may induce hyper cholesterolemia, hypertriglyceridemia and upregulation of CD36 and aortic PPARγ expression.

Aim To investigate the effects of activated renin angiotensin system in the vascular tissue on production of reactive oxygen species and nitric oxide.Methods Ten male Wuzhishan minipigs were divided into two groups,fed with normocholesterolemic(control;n=5) or hypercholesterolemic(2% cholesterol,hyperlipid,n=5) food for 3 months.Carotid arteries were isolated and the changes of TC,AngⅡ,ROS,NO and T-AOC of the vascular tissue were determined.In addition,hUVEC(human umbilical vein endothelial cell) were incubated with Ang I and chymostatin or with Ang I and losartan respectively for 24 h,the suspernatant was collected to measure the concentration of AngⅡ,ROS and NO. Results In comparison to the normocholesterolemic state,hypercholesterolemia led to a significant increase in TC content(108%±28%,P0.01),AngⅡ generation (115%±20%,P0.01) and ROS production(144%±28%,P0.01),but it led to a significant decrease in NO(51%±5%,P0.01) and T-AOC(56%±5%,P0.01).Cultrured hUVEC could convert Ang I to AngⅡ.AngⅡ increased by11 times in the presence of 10 nmol/L Ang I as compared with control group,and the level of ROS also increased while NO decreased significantly.When hUVEC were incubated with Ang I plus 100 or 500 μmol/L chymostatin,AngⅡ generation was reduced by 61%±6% or 65%±7% respectively,and the ROS decreased while NO increased significantly as compared with only Ang I.When hUVEC were incubated with Ang I plus Losartan,the generation of ROS and No was almost the same as compared with control group on condition that AngⅡ generation was not reduced. Conclusion hypercholesterolemia could activate renin angiotensin system of the vascular tissue,which results in an increased vascular tissue production of ROS and a decreased NO.This effect might be mediated by AT1 receptor.

目的 探讨制备泡沫细胞相关基因4多克隆抗体的方法.方法 将从人胎肝文库经聚合酶链反应扩增获得的泡沫细胞相关基因4基因全长cDNA序列,通过生物信息学分析,预测泡沫细胞相关基因4编码氨基酸序列的二级结构、抗原决定簇、功能结构域,并进行了多序列比对,并根据蛋白质的亲疏水性、二级结构、偶联难度及实验难度等,确定泡沫细胞相关基因4抗原13肽PKLVKEEVFWRNY,采用固相多肽合成法合成该抗原片段,偶联后经过基础免疫,6次加强免疫,经追踪检测,于第四次免疫后10～14天颈动脉放血,分离血清,冷冻抽干,-20℃保存而制备了泡沫细胞相关基因4兔抗人多克隆抗体.结果 用固相多肽合成法成功制备了泡沫细胞相关基因4兔抗人多克隆抗体,该抗体经间接酶联免疫吸附测定法检测其效价为1:16 000,Western blot检测可见40kDa处有目的带,用免疫组织化学方法从HepG2细胞中检测到泡沫细胞相关基因4蛋白表达,证实该抗体具有较好的反应性和特异性.结论 固相多肽合成法省时、省力、制备的抗体效价高;泡沫细胞相关基因4兔抗人多克隆抗体的制备,为进一步研究泡沫细胞相关基因4蛋白在动脉粥样硬化中的功能作用奠定了基础.

AIM: To assess the anti-proliferative effect of the extrac- tion from Hygrophila salicifolia on hepatocellular carcinoma (HCC) cells, and to provide the experimental evidence for the use of Hygrophila salicifolia extraction in the therapy of hepatocarcinoma. METHODS: Twenty-four female nude mice were subcu- taneously injected with human HCC cells. After 14 days, the mice were divided equally into control group, low dose Hygrophila salicifolia group, high dose Hygrophila salicifolia group and hydroxycamptothecin group. The volume and weight of tumor were measured 20 days after treatment. Histological changes of the xenografts and the serum levels of AST, ALT, LDH, and CRE were determined. RESULTS: In comparison with the control group, the tu- mor volume and weight of both Hygrophila salicifolia groups were decreased significantly (P<0.05). The tumor inhibiton rates were 46% and 62% (P<0.05) in low- and high- dose group, respectively. Histological examination results from Hygrophila salicifolia groups were almost the same as those from hydroxycamptothecin group. There were no obvious difference in the weights and the serum levels of AST, ALT, LDH, CRE the mice treated with Hygrophila salicifolia and those treated with hydroxycamptothecin. CONCLUSION: The extraction from Hygrophila salicifolia can significantly inhibit the growth of hepatocarcinoma cell xenografts in nude mice in a dose-dependent manner. Its effects on the general condition and on the functions of the heart, the liver and the kidney are almost the same as those of hydroxycamptothecin.

目的:探讨水蓑衣提取物对肝脏的保护作用.方法:40只雄性昆明种小鼠,随机分为对照组、CCl4损伤对照组、低剂量水蓑衣(45 g/kg)组、高剂量水蓑衣(90 g/kg)组和联苯双酯(150 mg/kg)组.除正常对照组外,其余动物均采用ip CCl4复制急性肝损伤小鼠模型,CCl4注射后16 h处死动物,测定各组小鼠血清ALT、AST的活性以及检查肝组织的病理改变,观察不同剂量水蓑衣对肝脏的保护作用.结果:与正常对照组相比,CCk损伤对照组动物血清ALT和AST活性显著升高(P＜0.01).肝脏出现轻度、中度和重度坏死及变性,低剂量水蓑衣组、高剂量水蓑衣组血清ALT和AST明显低于CCl4损伤对照组(ALT:1 485±755,1 211±528 vs 3 179±106;AST:2 045±293,2 052±386 vs 2 583±116,P＜0.01),与联苯双酯组相比无显著差异.水蓑衣能明显减轻CCl4引起的肝脏结构损害,高剂量水蓑衣的作用尤为突出,其作用优于联苯双酯.结论:水蓑衣呈剂量依赖性地减轻CCl4引起的肝损害,其作用优于联苯双酯.