Objective: Various materials have been used for tympanic membrane reconstruction in middle ear surgery. This study aimed to evaluate the difference between the tragal perichondrium and COOK artificial material in patients who underwent endoscopic type 1 tympanoplasty. Method: This retrospective study included patients who underwent endoscopic type 1 tympanoplasty from June 2021 to June 2022 at Sir Run Run Shaw Hospital, Zhejiang University School of Medicine. Patients were divided into group A (tragal perichondrium) and group B (COOK artificial material) according to the material used in the operation. All patients were followed up for 6 months. The differences in age, gender, operation site, disease course, preoperative air-bone gap (ABG), operation time, blood loss, hearing gain, and wound healing rate were compared between the two groups. Results: This study enrolled 197 patients, with 120 patients in group A and 77 patients in group B. There were no significant differences in age, gender, operation site, disease course, or preoperative ABG between groups A and B (p > 0.05). Both groups had significant postoperative improvement in hearing (group A: 30.98 +/- 9.58 dB vs. 17.07 +/- 9.92 dB, p < 0.001; group B: 29.75 +/- 7.52 dB vs. 14.25 +/- 9.07 dB, p < 0.001). The mean hearing gain in group A and group B was comparable (14.02 +/- 11.91 dB vs. 15.50 +/- 7.05 dB, p = 0.609). The wound healing rates of groups A and B were no differences (93.33% vs. 87.01%, p = 0.133). The patients in group B had a shorter operation duration (72.57 +/- 11.32 min vs. 61.86 +/- 9.27 min, p = 0.045) and less blood loss (12.38 +/- 3.7 mL vs. 8.10 +/- 2.43 mL, p = 0.004). Conclusions: Tragal perichondrium and COOK artificial material are reliable for functional and anatomical outcomes in endoscopic type 1 tympanoplasty, and COOK artificial material can save operation time and blood loss in surgery compared to the tragal perichondrium. (c) 2023 Zhejiang University. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/ by-nc-nd/4.0/).

Purpose: The current study aimed to investigate the role of long intergenic noncoding 01433 (LINC01433) in the proliferation, migration and invasion of nasopharyngeal carcinoma (NPC). Methods: Real-time quantitative PCR (RT-qPCR) was performed to determine the expressions of LINC01433 and miR-506-3p in NPC samples and cell lines. The effects of LINC01433 on cell proliferation, migration and invasion were measured by CCK-8, wound healing assay and Transwell, respectively. In addition, Pearson correlation analysis, starBase, RNA immunoprecipitation, luciferase assay, Western blot and functional experiments were conducted to detect and confirm the relationship between LINC01433 and miR-506-3p. Results: LINC01433 level was noticeably elevated in NPC tissues and cell lines. As the expression of LINC01433 in 5-8F cells was the highest in NPC cell lines and the expression of LINC01433 in SUNE1 cells was the lowest, 5-8F and SUNE1 cells were therefore selected as the target cells for following experiments. Furthermore, miR-506-3p was predicted as the target of LINC01433, and the two were negatively correlated with each other. Interestingly, overexpression of LINC01433 promoted proliferation, migration and invasion of NPC cells, while miR-506-3p reversed such effects of LINC01433. Moreover, LINC01433 silencing had the opposite effects to LINC01433 overexpression. Furthermore, miR-506-3p overexpression inhibited the expressions of MMP2, N-cadherin, p-PI3K and p-Akt, and promoted the expressions of E-cadherin and TIMP-2, and partially reversed the role of LINC01433 in promoting cancer development. Conclusion: The current findings reveal that LINC01433 regulates NPC cell biological progress through miR-506-3p.

Objective: Pseudocyst of the auricle is a benign cystic lesions of the ear. A variety of methods have been proposed priorly with different treatment effects. Although aggressive treatments may have good results, we aimed to introduce a less invasive method that also yield optimal cosmetic outcome. Patients and methods: From August 2019 to April 2020, a total of 32 patients with pseudocyst of the auricle were treated with a novel negative pressure drainage method. Results: The treatment has performed successfully in all patients. Only 2 patients experienced negative pressure drainage device detachment, and recovered smoothly after reinstallment. Patients were followed up for an average period of 3 months. The appearance of the auricle was recovered excellent in all patients. There were no postoperative complications or episodes of recurrence during the follow-up period. Conclusion: Considering the excellent cosmetic outcome and free of recurrence, we would recommend our negative pressure drainage method as a first-line treatment for all patients with auricular pseudocyst.

OBJECTIVE:To develop and validate a clinical score to predict the risk of tympanosclerosis before surgery. METHODS:A sample of 404 patients who underwent middle ear microsurgery for otitis media was enrolled. These patients were randomly divided into 2 cohorts: the training cohort (n = 243, 60%) and the validation cohort (n = 161, 40%). The preoperative predictors of tympanosclerosis were determined by multivariate logistic regression analysis and implemented using a clinical score tool. The predictive accuracy and discriminative ability of the clinical score were determined by the area under the curve (AUC) and the calibration curve. RESULTS:The multivariate analysis in the training cohort (n = 243, 60%) identified independent factors for tympanosclerosis as the female sex (odds ratio [OR]: 3.83; 95% CI: 1.66-9.37), the frequency-specific air-bone gap at 250 Hz ≥ 45 dB HL (OR: 3.68; 95% CI: 1.68-8.57), aditus ad antrum blockage (OR: 3.29; 95% CI: 1.38-8.43), type I eardrum calcification (OR: 25.37; 95% CI: 8.41-88.91) or type II eardrum calcification (OR: 18.86; 95% CI: 6.89-58.77), and a history of otitis media ≥ 10 years (OR: 4.10; 95% CI: 1.58-11.83), which were all included in the clinical score tool. The AUC of the clinical score for predicting tympanosclerosis was 0.89 (95% CI: 0.85-0.93) in the training cohort and 0.89 (95% CI: 0.84-0.95) in the validation cohort. The calibration curve also showed good agreement between the predicted and observed probability. CONCLUSIONS:The clinical score achieved an optimal prediction of tympanosclerosis before surgery. The presence of calcification pearls on the promontorium tympani is a strong predictor of tympanosclerosis with stapes fixation.

Nasopharyngeal carcinoma (NPC) at present is considered to be one of the fatal diseases detected commonly in the people belonging to Southeast Asia and southern China. According to the WHO reports among the detected cases of NPC worldwide, 80% are from China. The present study investigates the effect of tanshinone IIA on the migration and invasion potential of HNE-1NPC cells and studied the detailed mechanism involved. Effect of the tanshinone IIA on viability of the HNE-1NPC cells was analyzed by MTS assay. Cell matrigel invasion and wound-healing motility assays, respectively were used for the analysis of invasion and migration potential of HNE-1 cells. Tanshinone IIA inhibited the viability of HNE-1cells in a dose dependent manner. Migration and invasion potential of the tanshinone IIA treated cells was reduced significantly (P < 0.05) compared to the control cells after 48 h. Analysis of the proteins involved in migration and invasion revealed a significant decrease in the expression of matrix metalloproteinase (MMP)-2 and MMP-9 on treatment with tanshinone IIA. It also inhibited the p65 and p50 expression in the nuclear fractions of HNE-1 cells after 48 h. Thus, tanshinone IIA inhibits migration and invasion potential of the HNE-1NPC cells through reduction in the expression of matrix metalloproteinases. Therefore, tanshinone IIA can be used for the treatment of NPC.

颅骨脑膜的缺损引起蛛网膜下腔与鼻腔或鼻窦的沟通,脑脊液经漏口流出到颅外的鼻部即称为脑脊液(cerebrospinal fluid,CSF)鼻漏.脑脊液鼻漏是鼻内镜手术的严重并发症之一和颅底外科常见疾病之一.有文献报道在CSF鼻漏中,医源性者占20％,因其易导致颅内感染进而危及患者生命,故预防其发生以及在发生后采取及时有效的急诊治疗十分重要。

患者男,62岁,因右耳痒、痛11d,口角歪斜、步态不稳、吞咽困难、嗜睡3d于2011年6月20日急诊来我院.家属述患者既往有10余年糖尿病史,未经正规治疗.查体:意识不清,右外耳道皮肤红肿,右侧周围性面神经麻痹.实验室检查:血钾6.26 mmol/L,血糖27.6 mmol/L,血pH值7.275,血清碳酸氢盐28.0 mmol/L,β-羟丁酸8.5 mmol/L,剩余碱-9.7 mmol/L,C反应蛋白67.6 mg/L,白细胞22.0×109个/L,中性粒细胞比例93％,尿酮体(2+).腰穿脑脊液常规检查,未发现颅内感染.急诊以糖尿病酮症酸中毒合并Ramsay Hunt氏综合征收入我院内分泌科治疗.

患者 男,62岁,因右耳痒、痛11d,口角歪斜、步态不稳、吞咽困难、嗜睡3d于2011年6月20日急诊来我院.家属述患者既往有10余年糖尿病史,未经正规治疗.查体：意识不清,右外耳道皮肤红肿,右侧周围性面神经麻痹.实验室检查：血钾6.26 mmol/L,血糖27.6 mmol/L,血pH值7.275,血清碳酸氢盐28.0 mmol/L,β-羟丁酸8.5 mmol/L,剩余碱-9.7 mmol/L,C反应蛋白67.6 mg/L,白细胞22.0×109个/L,中性粒细胞比例93%,尿酮体（2＋）.腰穿脑脊液常规检查,未发现颅内感染.急诊以糖尿病酮症酸中毒合并Ramsay Hunt氏综合征收入我院内分泌科治疗.

Objective To approach application of fibrolaryngoscope in diagnosis and treatment of crypticity foreign body in hypopharynx. Method 112 patients who had indentify foreign body history in hypopharynx but the foreign body could not be found with common method were examined applying the fibrolaryngoscope form May 2001 to August 2006. Result The foreign bodies in hypopharynx were found and disloged successfully with fibrolaryngoscopic biopsy forceps for 111 patients. One patient was found granulation tissue in right sinus piriformis. Through cervical CT scanning, the foreign body was found in paraglotic space and disloged through discussion cervice. Conclusion Fibrolaryngoscope has the advantages of overall examination, clear observation, accuratissime location and convenient dislodgment. It can decrease missed dignosis in crypticity foreign body in hypopharynx.

患者 男，34岁，因“颈部外伤5h”于2004年10月1日17时入院。患者于5h前不慎被卷扬机高速旋转的皮带轮卷中颈部，出现颈部皮肤严重撕脱挫裂伤，气管、食管及颈前带状肌暴露。在当地医院行简单止血及气管断端气管插管后送我院急诊科。入院查体：意识清楚，颈正中气管漏口气管插管，呼吸平稳，颈前部严重挫伤，皮肤缺损面积约3cm×5cm，右侧颈内静脉断裂并自行扭转成球状，右侧迷走神经高位断裂，左侧喉返神经断裂，甲状软骨及环状软骨多处骨折、碎裂，喉腔暴露。双侧颈总动脉及左侧颈内静脉暴露，部分带状肌及部分胸锁乳突肌断裂或严重挫伤，气管在第5、6气管环之间完全断开，气管插管于气管断端的近心端，平第5气管环处食管壁1点钟位置到12点钟位置断裂，仅可见约不足1cm的食管黏膜仍有连接；后背部有两处皮肤挫伤，各长约15cm，宽约2cm，深达肌层。

Objective: To explore a safe, microtraumatic and effective theapeutic method when the ethmoid sinus invades orbital lamina and basicranial substance of bone. Methods: The study analysed the clinical data of 7 cases with osteofibroma in ethmoid sinus which invade orbital lamina and basicranial substance of bone from June 2001 to July 2003 by nasal entoscopy auxiliary image navigation. Results: Post-operation follow-up from six months to two years, none relapses, symptoms improve. Only one patient′s dura mater of brain was injuried which result to the cerebrospinal rhinorrhea. It was repaired successfully. Conclusions: Treating osteofibroma in ethmoid sinus which invade orbital lamina and basicranial substance of bone by nesal endoscopy auxiliary image navigation is a kind of effective method with low risk, microtrauma, and absolute resction of lesion.

一、临床资料 30例耳廓缺损患者中男21例,女9例;年龄13～69岁,平均35.7岁.缺损原因:外伤6例,耳廓乳头状瘤8例,耳廓纤维瘤3例,耳廓血管瘤4例,耳廓色素痣7例,基底细胞癌2例.缺损部位:耳甲腔12例,耳舟4例,耳轮及部分耳后7例,耳甲艇及对耳轮5例,三角窝2例.缺损大小:1.0 cm×1.2 cm～2.5 cm×3.0 cm.其中17例仅缺侧皮肤,8例缺侧皮肤及软骨膜,5例缺侧皮肤、软骨膜及部分软骨.

Objective: To explore the effect of modified radical operation with nasal endoscopy on non-invade the maxillary sinus mycosis. Methods: 95 cases of non-invade the maxillary sinus mycosis were treated with modified radical operation with nasal endoscopy. The maxillary cavity was cleaned through a small open in the anterior wall and then irrigated with solution of 5% PVP-I in and irrigated again after operation. Results: In the follow up from 6 months to 5 years, all cases were cured. Conclusion: With the modified radical maxillary sinusectomy under endoscopy, the disease tissue can be quite cleaned out and the function of nasal cavity and maxillary sinus can be preserved as many as possible. The recrudescence rate is low and the curative effect is fine.

回顾性分析1993年1月～2001年8月,我院收治的52例颈部外伤.报告如下.

气管狭窄多由外伤、肿瘤和手术等引起,临床治疗特别是低位气管狭窄治疗较为棘手.

患者,男,28岁.主诉咽部异物感伴口臭1年.1年前自觉咽部有异物感,进食后明显,可咳出食物残渣,口腔内有异味.但无吞咽困难及声嘶,无恶心、呕吐,无返酸、嗳气.体检:口咽稍红,双扁桃体体Ⅰ大小,无分泌物,会厌舌面可见淋巴组织,右侧梨状窝底部与左侧相比欠光滑.纤维喉镜检查:右侧梨状窝粘膜不光滑,有皱褶.CT检查:右侧会厌软骨旁有一1.5 cm×0.5 cm×1.1 cm大小含气及软组织密度区,其上端与喉腔气体影相通.吞钡造影示:右下咽憩室.于2000年11月28日全麻下行颈外进路右下咽憩室切除术.术中见右侧梨状窝外侧有一深1.5 cm、直径1.0 cm、内充满食物残渣及钡胶浆的囊袋,开口于右侧梨状窝外侧.手术完整切除囊袋,并放置负压引流管及鼻饲管.术后第1天拔除负压引流管,术后第6天拆线,第7天拔除鼻饲管,从口咽进食.术后病理:囊壁样组织一块,约1.5 cm×1.2 cm×0.1 cm,镜下示粘膜组织,表面被覆鳞上皮增生,间质内少量慢性炎细胞浸润.术后随访6个月,症状消失,未见复发.

混合瘤是唾液腺常见的良性肿瘤，由Billrothe于1859年首次描述，又名多形性腺瘤。该瘤发生于咽旁间隙者少见。我院于1996～2001年收治2例咽旁间隙巨大混合瘤，报告如下。