Abstract Background Anti-IgLON5 disease, a rare autoimmune neurological disorder, remains understudied in Eastern populations. This study aimed to characterize the clinical characteristics, treatment responses, and long-term outcomes of Chinese patients with anti-IgLON5 disease in a multicenter Chinese cohort. Methods This retrospective multicenter study enrolled 24 patients with anti-IgLON5 disease confirmed by serum and/or cerebrospinal fluid antibody testing from 17 centers in China. Human leukocyte antigen (HLA) typing was performed on patient blood samples. Clinical characteristics, mRS/ICS outcomes, treatment response, relapse, and long-term outcomes were analyzed. Short-term response was defined as improvement in mRS from admission to discharge, and relapse was defined as recurrence or worsening of symptoms after initial clinical improvement. Results The mean age at onset was 59.6 years. Among 18 patients who received immunotherapy, 10/18 (55.6%) met the definition of achieving a short-term response. Responders included more women (6/10 vs. 2/8), fewer patients with bulbar symptoms (4/10 vs. 5/8), and more frequently had HLA-DRB1*10:01 or HLA-DQB1*05:01 (8/10 vs. 4/8) compared with non-responders. Men aged ≥ 65 years had poorer outcomes, whereas younger men and most women responded well. Long-term follow-up was performed on 16 patients (median 18 months, range 3–60 months), and eight withdrew from follow-up. Relapse occurred in 4/16 patients (25.0%). It typically occurred about 6–12 months after discharge, often following abrupt treatment withdrawal, and was effectively controlled with re-treatment. Kaplan–Meier analysis indicated that relapse risk was the highest within the first 6 months post-discharge. Early treatment (≤ 6 months) tended to be associated with more favorable outcomes. Overall, 10/14 immunotherapy-treated patients with long-term follow-up (71.4%) showed improvement, and 7/14 (50.0%) became asymptomatic. In this cohort, HLA-DQA1*01:05 and HLA-DRB1*10:01/HLA-DQB1*05:01 were over-represented among the typed patients, and the response rate to immunotherapy was numerically higher than that in the Western population (55.6% vs. 40%). The misdiagnosis rate was 33.3%. The median diagnostic delay was 2 months (IQR, 1–12 months; range, 2 days–6 years). Conclusions As the largest cohort of anti-IgLON5 disease in China to date, this multicenter series demonstrated that Chinese and Western patients with anti-IgLON5 disease have both shared and distinct clinical characteristics. Chinese patients exhibited relatively favorable responses to early immunotherapy, relapse susceptibility requiring prolonged treatment, and potential HLA associations, offering new insight into disease management.

Prostate cancer (PCa) is an extremely heterogeneous cancer and is highly prevalent in the older male population. Since intra-tumour heterogeneity (ITH) commonly results in PCa chemotherapy resistance and recurrence, it is critical to explore its effects on tumour behaviour. Prognostic genes related to ITH were identified, and a signature was constructed using Cox regression analyses and multiple machine learning algorithms. Single-cell RNA sequencing data extracted from PCa and CRPC samples were analysed via sub-clustering, pseudotime, cell communication and drug sensitivity approaches to elucidate their function. The oncogenic potential of hub genes was confirmed by immunohistochemistry and cell proliferation assays. An 11-gene signature underlying a prostate cancer meta-program (PCMP) was generated by selecting an optimal combination of machine learning methods. Survival assays and multivariate Cox regression analyses conducted in multiple cohorts revealed the superior prognostic value of the PCMP signature. Functional enrichment analyses indicated that it dysregulates the cell cycle. Using trajectory and cell-cell communication analyses, we illustrated that PCMP genes exert oncogenic effects by enhancing the proliferation and oxidative phosphorylation of epithelial cells. Intra-cellular assays also demonstrated that CENPA and CKS1B had promising malignant potential. In summary, our research not only establishes the association between the PCMP signature and reveals its malignant characteristics, but also deepens our understanding of the mechanisms underlying PCa progression and ITH. It holds promise for the development of targeted therapeutic interventions, thereby offering clinical benefits to patients.

BackgroundCuproptosis, along with RNA methylation regulators, has recently come to the fore as innovative mechanisms governing cell death, exerting profound impact on the onset and progression of multiple cancers. Nonetheless, the prognostic implications and underlying regulatory mechanisms of them associated with prostate cancer (PCa) remain to be thoroughly investigated.MethodsGenomic and clinical data for PCa from The Cancer Genome Atlas datasets were analyzed to identify a prognostic model through univariate and Least Absolute Shrinkage and Selection Operator Cox regression analyses that were validated utilizing external datasets. We used receiver operating characteristic curves and C-index to evaluate the accuracy of our prognostic model. In conjunction with this, we conducted single-cell RNA sequencing (scRNA-seq) analyses to investigate underlying mechanisms and evaluate the degree of immune infiltration, as well as to assess patients’ responses to diverse chemotherapy agents. Especially, qPCR assay was utilized to unveil the expression of signature genes in PCa.ResultsWe meticulously selected six Cuproptosis-Associated RNA Methylation Regulators (CARMRs) to establish a risk prognosis model, which was further verified to obtain enhanced predictive capacity in external validation cohorts. Insights from immune infiltration and scRNA-seq analyses have elucidated the immune characteristics of PCa, and highlighted the immunosuppressive role of regulatory T cells on immune response. Additionally, drug susceptibility analysis demonstrated that patients with PCa in the low-risk category derived better benefit from bicalutamide treatment, whereas those in the high-risk group exhibited a favor response to adriamycin and docetaxel treatments. The qPCR and immunohistochemistry (IHC) staining assays also reveal the a dramatically altered expression pattern of TRDMT1 and ALYREF in PCa tissues.ConclusionIn general, we established a model involving CARMRs that can better predict the risk of recurrence of PCa and have identified the possible mechanisms affecting PCa progression, thereby promoting further research in this field.

Background: As a highly prevalent tumor in males, prostate cancer (PCa) needs newly developed biomarkers to guide prognosis and treatment. However, few researches have elaborated on the function of cuproptosis-associated RNA methylation regulators (CARMRs). Methods: We identified CARMRs based on single-sample gene set enrichment analysis and weighted gene co-expression network analyses. Subsequently, we performed 10 machine learning algorithms and 101 combinations of them to select the best model in TCGA, GSE70768, GSE70769, and DKFZ cohorts. Furthermore, we explored the potential function of CARMRs in the tumor microenvironment, immunotherapy, and tumor mutation burden (TMB). We validated the expression of the two genes with the largest regression coefficients using qRT-PCR. Results: In our analysis, we successfully established a consensus prognostic model with 9 CARMRs based on the 101-machine learning framework. Furthermore, functional enrichment analysis revealed different metabolic and signaling pathways in the high- and low-risk groups. Notably, the high-risk group had a higher TMB, a lower level of immune infiltration, and a lower expression of immune checkpoints. Through drug sensitive analysis, we screened chemotherapy drugs suitable for different groups. Vitro experiments illustrated the high expression of C4orf48 and SLC26A1 in PCa compared with normal controls. The discovery was in concordance with bioinformatic analysis results. Conclusion: A gene signature with 9 CARMRs was developed in our study, which served as biomarkers for PCa. This brings benefits in determining the prognosis of patients with PCa and guiding personalized treatment.

Photodynamic therapy (PDT) has emerged as a promising cancer treatment approach due to its non-invasive and specifically targeted nature. However, the effectiveness of PDT is hindered by the complex synthesis of conventional photosensitizers and inadequate reactive oxygen species (ROS) generation. Here, we synthesize a copper-doped Bi2S3 (Cu-Bi2S3) nanorod to investigate its PDT potential against PCa. Compared with bulk Bi2S3 (58%), Cu-Bi2S3 nanorod caused 87% of PC3 cells to die under light. The dispersed Cu in the Bi2S3 bulk phase effectively inhibits the recombination of photogenerated electron-hole pairs, ultimately providing a high concentration of charge carriers. DFT calculations show that Cu doping causes the d-band center of Cu-Bi2S3, promoting the adsorption and activation of O2 on Cu-Bi2S3 to enhance ROS generation. This work offers a viable solution to the pressing scientific challenge of ROS generation, a key aspect of enhancing the efficacy of PDT for cancer treatment.

Clear cell renal cell carcinoma (ccRCC), the most common type of renal cell carcinoma (RCC), is not sensitive to traditional radiotherapy and chemotherapy. The polyphenolic compound Gallic acid (GA) can be naturally found in a variety of fruits, vegetables and plants. Autophagy, an intracellular catabolic process, regulates the lysosomal degradation of organelles and portions in cytoplasm. It was reported that autophagy and GA could affect the development of several cancers. Therefore, the aim of the present study was to evaluate the effects of GA on ccRCC development and clarify the role of autophagy in this process. In the present study, the effects of GA on the proliferation, migration and invasion of ccRCC cells were investigated in vitro by Cell Counting Kit-8, colony formation, flow cytometry, wound healing and Transwell migration assays, respectively. Additionally, the effects of GA on ccRCC growth and metastasis were evaluated using hematoxylin-eosin and immunohistochemical staining in vivo. Moreover, it was sought to explore the underlying molecular mechanisms using transmission electron microscopy, western blotting and reverse transcription-quantitative PCR analyses. In the present study, it was revealed that GA had a more potent viability inhibitory effect on ccRCC cells (786-O and ACHN) than the effect on normal renal tubular epithelial cell (HK-2), which demonstrated that GA selectively inhibits the viability of cancer cells. Furthermore, it was identified that GA dose-dependently inhibited the proliferation, migration and invasion of ccRCC cells in vitro and in vivo. It was demonstrated that GA promoted the release of autophagy markers, which played a role in regulating the PI3K/Akt/Atg16L1 signaling pathway. All the aforementioned data provided evidence for the great potential of GA in the treatment of ccRCC.

Clear cell renal cell carcinoma (ccRCC), the most common type of renal cell carcinoma (RCC), is not sensitive to traditional radiotherapy and chemotherapy. The polyphenolic compound Gallic acid (GA) can be naturally found in a variety of fruits, vegetables and plants. Autophagy, an intracellular catabolic process, regulates the lysosomal degradation of organelles and portions in cytoplasm. It was reported that autophagy and GA could affect the development of several cancers. Therefore, the aim of the present study was to evaluate the effects of GA on ccRCC development and clarify the role of autophagy in this process. In the present study, the effects of GA on the proliferation, migration and invasion of ccRCC cells were investigated in vitro by Cell Counting Kit‑8, colony formation, flow cytometry, wound healing and Transwell migration assays, respectively. Additionally, the effects of GA on ccRCC growth and metastasis were evaluated using hematoxylin‑eosin and immunohistochemical staining in vivo. Moreover, it was sought to explore the underlying molecular mechanisms using transmission electron microscopy, western blotting and reverse transcription‑quantitative PCR analyses. In the present study, it was revealed that GA had a more potent viability inhibitory effect on ccRCC cells (786‑O and ACHN) than the effect on normal renal tubular epithelial cell (HK‑2), which demonstrated that GA selectively inhibits the viability of cancer cells. Furthermore, it was identified that GA dose‑dependently inhibited the proliferation, migration and invasion of ccRCC cells in vitro and in vivo. It was demonstrated that GA promoted the release of autophagy markers, which played a role in regulating the PI3K/Akt/Atg16L1 signaling pathway. All the aforementioned data provided evidence for the great potential of GA in the treatment of ccRCC.

Prostate diseases are common and frequently-occurring diseases in males. Nanomedicine is the interdisciplinary integration of medicine and materials science that brings great opportunities to the biomedical field by designing and manufacturing biomaterials to satisfy clinical practice needs. Gels are a very promising material with a wide range of applications in the biomedical field. There are numerous research reports on the important role of gels in drug delivery and release, anti-inflammation and anti-infection, promotion of tissue regeneration, and wound healing. In this review, we overview prostate diseases and the classification of gels and focus on the current research on gels-based nanocomposites in the diagnostics and therapy of prostate diseases. Moreover, the shortcomings of gels-based nanocomposites for prostate diseases are pointed out, and we look forward to a broader prospect of the clinical application of gels-based nanocomposites in prostate diseases in the future, which provides a new strategy for diagnostics and therapy of prostate diseases.

A retrospective study was performed on 200 patients who underwent miniaturized percutaneous nephrolithotomy (mini-PCNL) or retrograde intrarenal surgery (RIRS) for 10–20 mm sized lower pole renal calculi to investigate the relationship between computed tomography (CT) attenuation of calculi and surgical outcomes. CT was used to examine the location, size, and CT attenuation values of the calculi. Additionally, the operation time, hospital stay, hemoglobin (Hb) reduction, stone-free rate (SFR), and complication rate were also meticulously documented and subjected to comparative analysis. Complications were assessed using the Clavien-Dindo grading system. We observed no significant differences in hospitalization data and follow-up outcomes, except for a longer hospital stay and higher Hb drops in patients receiving mini-PCNL. Statistical analysis revealed an association between CT attenuation and operation time. Compared with mini-PCNL, RIRS could reduce bleeding, hospital stay, surgery time, and complications for 10–20 mm sized lower pole kidney stones with CT values < 1000 HU. RIRS resulted in longer operation time and lower stone-free rates despite shorter hospital stays and less bleeding than mini-PCNL for stones with CT values > 1000 HU. Therefore, selecting an appropriate surgical method based on CT attenuation might improve outcomes. For patients with stone attenuation values < 1000 HU, RIRS is the recommended option. When stone attenuation values > 1000 HU, the surgical method should be chosen based on the patient's individual situation.

Clear cell renal cell carcinoma (ccRCC) has a high metastatic rate, and its incidence and mortality are still rising. The aim of this study was to identify the key tumor-infiltrating immune cells (TIICs) affecting the distant metastasis and prognosis of patients with ccRCC and to construct a relevant prognostic panel to predict immunotherapy response. Based on ccRCC bulk RNA sequencing data, resting mast cells (RMCs) were screened and verified using the CIBERSORT algorithm, survival analysis, and expression analysis. Distant metastasis-associated genes were identified using single-cell RNA sequencing data. Subsequently, a three-gene (CFB, PPP1R18, and TOM1L1) panel with superior distant metastatic and prognostic performance was established and validated, which stratified patients into high- and low-risk groups. The high-risk group exhibited lower infiltration of RMCs, higher tumor mutation burden (TMB), and worse prognosis. Therapeutically, the high-risk group was more sensitive to anti-PD-1 and anti-CTLA-4 immunotherapy, whereas the low-risk group displayed a better response to anti-PD-L1 immunotherapy. Furthermore, two immune clusters revealing distinct immune, clinical, and prognosis heterogeneity were distinguished. Immunohistochemistry of ccRCC samples verified the expression patterns of the three key genes. Collectively, the prognostic panel based on RMCs is able to predict distant metastasis and immunotherapy response in patients with ccRCC, providing new insight for the treatment of advanced ccRCC.

Radical prostatectomy is a primary treatment option for localized prostate cancer (PCa), although high rates of recurrence are commonly observed postsurgery. Photodynamic therapy (PDT) has demonstrated efficacy in treating nonmetastatic localized PCa with a low incidence of adverse events. However, its limited efficacy remains a concern. To address these issues, various organic polymeric nanoparticles (OPNPs) loaded with photosensitizers (PSs) that target prostate cancer have been developed. However, further optimization of the OPNP design is necessary to maximize the effectiveness of PDT and improve its clinical applicability. This Review provides an overview of the design, preparation, methodology, and oncological aspects of OPNP-based PDT for the treatment of PCa.

OBJECTIVE:To investigate the effect of inflammation-related genes on the prognosis of prostate cancer (PCa).METHODS:We downloaded PCa-related clinical data and mRNA sequencing data from the database Cancer Genome Atlas (TCGA) and inflammation-related pathway gene sets from MsigDB. Using univariate regression and LASSO regression analyses, we screened inflammation-related genes for the construction of a prognostic risk model and evaluated the performance of the model in predicting the prognosis of PCa by Kaplan-Meier and ROC analyses. Based on the nomogram, we calculated the risk scores of the patients, divided them into a high-risk and a low-risk group based on the median values of their risk scores, identified differentially expressed genes for enrichment analysis and verified the expression level of SPHK1 in the PCa tissue microarrays by immunohistochemical staining.RESULTS:Totally 19 inflammation-related genes were identified from 172 candidate genes for the construction of the prognostic risk model, including the risk genes CD14, PIK3R5, GABBR1, RELA, IRF7, SCARF1, MSR1, SPHK1, OSM and STAB1, and the protective genes AQP9, LPAR1, ATP2C1, NDP, CXCL6, P2RY2, DCBLD2, PCDH7, and IFNAR1. Kaplan-Meier analysis showed that the patients with high risk scores had a significantly lower recurrence-free survival and a worse prognosis than those with low risk scores. Differentially expressed genes were involved mainly in the activation of inflammatory response pathways. Immunohistochemical results indicated that the expression of SPHK1 was significantly higher in the tumorous than in the normal tissue and increased with the Gleason score. There was a correlation between the SPHK1 expression and envelope invasion.CONCLUSION:The prognostic risk model of inflammation-related genes constructed based on the TCGA database can effectively predict the prognosis of PCa.

目的 探讨无线超高清腔镜系统应用于经皮肾镜取石术(PCNL)的有效性、安全性和便利性.方法 选取2021年5月-2021年7月安徽医科大学第一附属医院收治的肾结石患者74例,分为有线腔镜组(n=38,使用传统有线腔镜系统行PCNL)和无线腔镜组(n=36,使用无线超高清腔镜系统行PCNL).患者年龄18～70岁,肾结石最大长径20～40 mm,肾功能正常.比较两组患者手术有效性、安全性和操作便利性的差异.结果 两组患者均顺利完成手术,两组患者手术时间、血红蛋白下降值、并发症发生率、术后住院时间和一期结石清除率分别为(44.45±12.04)和(43.78±10.11)min(P=0.797)、(10.05±3.45)和(9.78±4.24)g/L(P=0.760)、47.4％和52.8％(P=0.642)、(5.32±1.25)和(5.19±1.04)d(P=0.652)、89.5％和91.7％(P=0.747),两组患者比较,差异均无统计学意义(P>0.05).结论 无线超高清腔镜系统用于PCNL安全有效.相较传统有线腔镜系统,其没有光源线和摄像头电缆线的限制,操作上更加便利,值得在PCNL中推广应用.

Background We aimed to systematically identify novel susceptible factors related to the occurrence and development of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS)-like symptoms that were not limited to lifestyles or dietary habits in Chinese population. Methods We recruited participants from three centers (Shanghai [northeast], Hefei [east], and Lanzhou [northwest]) from August 2020 to June 2021. Demographics, lifestyles, dietary habits, past medical history, and national institutes of health-chronic prostatitis symptom index (NIH-CPSI) were collected from the individuals via optimized questionnaires. Logistic regression analysis and multivariate adjustment models were used to calculate the odds ratio (OR) and 95% confidence interval (95% CI) to assess the association between these variables and CP/CPPS-like symptoms. Results A total of 1851 participants were enrolled in this study (764 cases and 1087 controls). Age distributions differed between groups (median, range: 32, 18-74 vs. 29, 18-70, p < 0.001). After adjustment, physicochemical occupational hazards were identified significantly related to CP/CPPS-like symptom occurrence and development (ORoccurrence: 1.389, 95% CI: 1.031-1.870, p < 0.001; ORdevelopment: 2.222, 95% CI: 1.464-3.372, p < 0.001); besides, greater than or equal to four ejaculations per week significantly increased the likelihood of CP/CPPS-like symptoms compared with one ejaculation per week (ORoccurrence: 3.051, 95% CI: 1.598-5.827, p = 0.001). For these patients, who were easily felt gastrointestinal discomfort caused by spicy food intake, they had a higher incidence to affect with CP/CPPS-like symptoms (ORoccurrence: 2.258, 95% CI: 1.858-2.745, p < 0.001). In addition, history of drug allergy and genitourinary infections were identified as independent susceptible factors for the occurrence of CP/CPPS-like symptoms (ORoccurrence: 1.689, 95% CI: 1.007-2.834, p = 0.047; ORoccurrence: 3.442, 95% CI: 2.202-5.382, p < 0.001, respectively), while the history of rheumatic immune diseases was found tightly associated with the development of CP/CPPS-like symptoms (ORdevelopment: 2.002, 95% CI: 1.008-4.058, p = 0.048). Conclusion Infection/inflammatory/immune-related disorders, novel dietary habits, and lifestyles associated with the susceptibility of CP/CPPS-like symptoms' occurrence and development are identified. Altering these irregular conditions serves as potential strategies for the treatment of patients with CP/CPPS-like symptoms.

Objective To develop and validate a nomogram for predicting renal dysfunction in patients with simple renal cysts (SRCs). Methods We performed a multivariable logistic regression analysis of an in-hospital retrospective cohort of patients with SRCs in the Urology Department of the First Affiliated Hospital of Anhui Medical University. For prognostic model development, 386 patients with SRCs were enrolled from January 2016 to December 2018. External validation was performed in 46 patients with SRCs from January 2019 to April 2019. The primary outcome was renal dysfunction. Results Patients were divided into normal or abnormal estimated glomerular filtration rate groups (293 vs. 93) based on the cut-off value of 90 mL/minute/1.73 m 2 . Logistical regression analysis determined that age, haemoglobin, globulin, and creatinine might be associated with renal dysfunction, and a novel nomogram was established. Calibration curves showed that the true prediction rate was 77.42%, and decision curve analysis revealed that the nomogram was more effective with threshold probabilities ranging from 0.1 to 0.8. The area under the curves were 0.829, 0.752, and 0.888 in the overall training, internal, and external validation cohorts, respectively. Conclusions We established a nomogram to predict the probability of developing renal dysfunction in patients with SRCs.

Background:Prostate cancer (PCa) ranks as the most common malignancy and the second leading cause of cancer-related death among males worldwide. The essential role of autophagy in the progression of PCa and treatment resistance has been preliminarily revealed. However, comprehensive molecular elucidations of the correlation between PCa and autophagy are rare.Method:We obtained transcription information and corresponding clinicopathological profiles of PCa patients from TCGA, MSKCC, and GEO datasets. LAASO analysis was employed to select gene signatures and estimate the autophagy score for each patient. Correlations between the signature and prognosis of PCa were investigated by K-M and multivariate Cox regression analyses. A nomogram was established on the basis of the above results. Further validations relied on ROC, calibration analysis, decision curve analysis, and external cohorts. Variable activated signaling pathways were revealed using GSVA algorithms, and the genetic alteration landscape was elucidated via the oncodrive module from the "maftools" R package. In addition, we also examined the therapeutic role of the signature based on phenotype data from GDSC 2016.Result:Six autophagy-related genes were eventually selected to establish the signature, including ULK1, CAPN10, FKBP5, UBE2T, NLRC4, and BNIP3L. We used these genes and corresponding coefficients to calculate an autophagy score (AutS) for each patient in this study. A high AutS group and a low AutS group were divided on the mean AutS of the patients. Longer overall survival, higher Gleason score and PSA, and better response to ADT were observed in patients with high AutS. Meanwhile, we found that high AutS PCa was related to more proliferation-associated signaling activation and higher genetic mutation frequencies, manifesting a poor prognosis. A nomogram was constructed based on GS, T stage, PSA, and AutS as covariates. Its discriminative efficacy and clinical value were validated using robust statistical methods. Finally, we tested its prognostic value through two external cohorts and six published signatures.Conclusion:The autophagy-related gene signature is a highly discriminative model for risk stratification and drug therapy in PCa, and a nomogram incorporating AutS might be a promising tool for precision medicine.

Objective:To explore the experiences of using wireless intelligent endoscopy system in rigid ureteroscopy and flexible fiberscopy for urinary calculi.Methods:80 ureteral stone patients who needed the treatment of rigid ureteroscopy or flexible fiberscopy were included, 40 cases were treated by conventional ureteroscopy: rigid ureteroscopy (20 cases) and flexible fiberscopy (20 cases) , 40 cases were treated by wireless intelligent ureteroscopy: rigid ureteroscopy (20 cases) and flexible fiberscopy (20 cases). The differences in preoperative preparation time, operative time, clarity, grip sense and image delay between conventional ureteroscope groups and flexible fiberscope groups were compared.Results:There was no statistically significant difference in preoperative preparation time, operative time, clarity, image delay or intraoperative grip feeling between the conventional rigid ureteroscopy group and the wireless intelligent rigid ureteroscopy group (P>0.05). There were no statistically significant differences in preoperative preparation time, operation time, clarity and image delay between the conventional flexible ureteroscopy group and the wireless intelligent flexible ureteroscopy group (P>0.05). The grip feeling of the wireless intelligent flexible ureteroscope group was better than that of the traditional flexible fiberscopy group, and the comparison between the two groups was statistically significant (P<0.05).Conclusion:Wireless intelligent abdominal surgery system is a safe and effective laparoscopic system in the treatment of endoscopic calculi, which can improve the grip feeling of the endoscopic lens and reduce the sense of fatigue of the operator, and has a wide application prospect.

2015年,中国工程院郭应禄院士团队在生命科学第三次革命浪潮中首次提出"微能量医学"的概念.微能量医学利用体外设备产生具有生物学效应的机械波或电磁波,以此对疾病或亚健康状态进行预防和治疗[1].微能量治疗具有激活、转化和招募干细胞,参与靶器官、组织的修复和再生,增加局部血液循环,刺激周围神经生长,抗炎和镇痛等作用.与传统疗法相比,微能量疗法作为一种安全的治疗手段,不会产生药物相关副作用和手术相关并发症[2].冲击波、超声波和电磁场是微能量治疗中常见的应用介质[1].

Objective:To explore the value of robot-assisted laparoscopy in the treatment of ureteropelvic junction obstruction (UPJO).Methods:Medical records were collected from 99 patients who underwent robot-assisted ureteropelvic dissociation plasty in our hospital from October 2014 to December 2019, including 74 males and 25 females, with an average age of (28±15) years. There were 54 cases with left side UPJO, 40 cases right side and 5 cases bilateral side. 16 cases were treated with concurrent diseases during operation, including 13 cases with ipsilateral urolithiasis.Results:All the 99 patients received the robot-assisted ureteropelvic dissociation plasty, and the disease was treated simultaneously. The operative time was (179±58) min. The time to drainage and catheter removal was (5.2±2.0) days and (7.5±2.7) days. No intraoperative complications occurred, no conversion to open surgery. Clavien classification-Dindo (grade I-Ⅱ) 13 cases (13.1%). The average length of stay was (14±4) days and the average postoperative hospital stay was (7.0±2.2) days. The patients were followed up for (11.9±0.4) months without recurrence.Conclusion:Robot-assisted laparoscopic surgery for the treatment of UPJO has the advantages of less trauma, clear operative field, high success rate of surgery, quick recovery and fewer complications, and can simultaneously deal with complicating diseases, with obvious clinical application advantages.