Feline panleukopenia is an acute, highly contagious, and fatal infectious disease, posing a serious threat to the health and conservation of felids. It is necessary to develop diagnostic tools suitable for point-of-care testing (POCT). Herein, a colorimetric loop-mediated isothermal amplification (LAMP) assay targeting the FPV VP2 gene was developed using cresol red as a colorimetric indicator. The colorimetric LAMP enables result interpretation by visual observation of a color change from violet to yellow after amplification at 64 °C for 40 min. The limit of detection was 18.38 copies/µL for pMD-VP2 plasmid and 101.62 TCID50/mL for crudely extracted nucleic acids. This method exhibits no cross-reactivity with other common feline pathogens, and shows good repeatability. Nevertheless, this method is unable to differentiate FPV from canine parvovirus (CPV) owing to their high genetic similarity. Testing of 153 clinical samples revealed a 40.52% (62/153) positive rate, with 98.04% agreement compared to a commercial qPCR kit (42.48% positive rate). Collectively, the developed colorimetric LAMP assay offers a specific, sensitive, simple and visual approach for FPV detection, demonstrating great potential for field point-of-care applications.

Background: Coagulase-negative staphylococci, such as Staphylococcus cohnii are increasingly recognized as crucial reservoirs of antimicrobial resistance (AMR) genes. However, their role in the dissemination of multidrug resistance within livestock populations, which represents a potential threat to public health through the food chain, remains insufficiently investigated. Aim: The aim of this study was to characterize the AMR profiles of S. cohnii isolates from Chinese swine farms using integrated phenotypic and genotypic approaches. Methods: This study characterized 32 porcine-derived S. cohnii isolates from Jilin Province, China. Antimicrobial susceptibility testing against 20 antibiotics was performed using the agar dilution method, and the genomic basis of resistance was investigated through second-generation sequencing followed by comprehensive antibiotic resistance gene analysis. Results: Phenotypic testing revealed universal resistance (100%) to 11 antibiotics, including ampicillin and tetracycline. Genomic analysis identified 32 distinct resistance genes, with six of them (e.g., fusF, salE, vanTG) present in all isolates. Critically, multiple transferable multidrug-resistant genes were detected, including ermB (53.13%), ermC (18.75%), and cfrA (12.5%). Furthermore, silent glycopeptide resistance genes (e.g., vanTG, vanYB) were identified despite phenotypic susceptibility to vancomycin. Conclusion: This study establishes porcine S. cohnii as a significant reservoir of diverse and mobile resistance genes, including silent determinants with potential for horizontal transfer. These findings underscore the necessity of integrating genomic surveillance of commensal staphylococci in livestock within a One Health framework to mitigate the dissemination of resistance to human pathogens.

Feline herpesvirus type 1 (FHV-1) is one of the major pathogens causing upper respiratory tract disease (URTD) in cats, and is widely prevalent among domestic cats and other felids worldwide. Herein, we developed a closed one-tube colorimetric and fluorescent LAMP assay targeting the FHV-1 TK gene, using cresol red as the colorimetric indicator and EvaGreen as the fluorescent dye. The assay was completed within 40 min at 63 °C, with results readable either by colorimetric observation or fluorescence amplification curve. FHV-1-positive reaction could be confirmed by a color change from violet to yellow with the naked eye, and also could be determined by fluorescence amplification curve. The assay showed no cross-reactivity with other related pathogens, and had a detection limit of 100 copies. Among 87 clinical nasal swabs tested, 44 were positive, achieving an overall agreement of 97.7% compared to TaqMan quantitative PCR. The newly developed LAMP assay is simple, efficient, result-visual and reliable, which can be applied in the clinical diagnosis and epidemiological investigation of FHV-1, especially for point-of-care testing of FHV-1 in primary veterinary clinics and field settings.

Background: Streptococcus pluranimalium is an emerging zoonotic pathogen. Its overall low isolation rate has contributed to the paucity of available data. However, data on its antimicrobial resistance (AMR) profile, particularly from swine in China, remain scarce. The escalating global challenge of AMR underscores the urgent need to elucidate resistance mechanisms in zoonotic veterinary pathogens. Aim: To the best of our knowledge, this is the first comprehensive characterization of AMR phenotypes and genotypes in S. pluranimalium isolates from diseased pigs in China, with an emphasis on identifying novel resistance gene combinations and evaluating their potential for cross-species dissemination. Methods: The AMR profile of 17 S. pluranimalium isolates was determined using minimum inhibitory concentration testing against a panel of antimicrobials. Next-generation sequencing was used to comprehensively investigate the genetic determinants of resistance, and a comparative analysis was conducted incorporating 5 publicly available genomes. Results: All 17 isolates were multidrug-resistant. They exhibited high resistance rates to sulfamethoxazole (100%), azithromycin (94.12%), tylosin tartrate (70.59%), and tetracycline (76.47%) but remained susceptible to penicillin, amoxicillin, ceftizoxime, and vancomycin. Genomic analysis identified 20 antimicrobial resistance genes. Notably, we report for the first time the co-occurrence of five aminoglycoside resistance genes (aad(6), aph(3')-IIIa, sat-4, aph(2'')-Ia, and patB) in this species, along with the simultaneous detection of lnuB and lnuC. Conclusion: Our next-generation sequencing analysis identified diverse and novel combinations of antibiotic resistance genes in porcine-derived S. pluranimalium, positioning it as a potential reservoir for AMR dissemination. These findings offer critical molecular insights into the AMR landscape in Chinese swine, emphasizing the risk of cross-species transmission and the need for integrated surveillance of this pathogen.

Salmonella Typhimurium is a major cause of foodborne illness. This study investigated the antibacterial activity, characterization, and mode of action of bacteriocin-like inhibitory substances (BLIS) produced by Levilactobacillus brevis PG-11, isolated from traditional Chinese kimchi. The BLIS of L. brevis PG-11 exhibited strong antibacterial activity against S. Typhimurium ATCC 14028. It also exhibited broad-spectrum antimicrobial activity against various foodborne and human pathogens, including Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus epidermidis. The minimum inhibitory concentration (MIC) of the BLIS against E. coli, S. Typhimurium, and S. aureus was 62.5, 125, and 125 mg/ml. The active antibacterial substances were partially purified using dialysis and tricine-SDS-PAGE, revealing a molecular weight of less than 1 kDa for the active antibacterial substances. Furthermore, the BLIS was stable under acidic pH (pH 3.0-6.0), heat treatment (121°C, 20 min), UV radiation (4 h), and sensitivity to papain. Maximum antibacterial activity was observed during the stationary growth phase after 32 h of culture. Propidium iodide (PI) uptake, ATP leakage, and scanning electron microscopy (SEM) confirmed that BLIS disrupted cell membrane integrity, leading to cell membrane damage pore formation, and bacterial growth inhibition. These results highlight the potential of L. brevis PG-11 BLIS as a natural biological preservative for food preservation applications.

Background:The emergence of Aeromonas salmonicida in swine respiratory disease represents a significant animal health concern with potential public health implications due to the risk of zoonotic antimicrobial resistance transmission. Aim:This study aimed to characterize the phenotypic and genotypic antimicrobial resistance profiles of A. salmonicida isolated from pigs with respiratory disease in Jilin Province, China, to assess its potential threat to animal and public health. Methods:Between January 2023 and December 2023, 786 pigs with mild respiratory disease were randomly sampled from 19 conveniently selected farms in Jilin Province, China. The presence of one or more mild clinical signs, such as nasal discharge, coughing, labored breathing, lethargy, or anorexia, defines this condition. From these pigs, 13 A. salmonicida strains were isolated from the upper respiratory tracts. The isolates' taxonomic identification was systematically determined through a polyphasic approach. This included 16S rRNA gene sequencing, phylogenetic analyses based on the gyrB and rpoB housekeeping genes, and Average Nucleotide Identity analysis. Antimicrobial susceptibility testing against 21 agents was performed using the CLSI-standardized agar dilution minimum inhibitory concentration assay, with Escherichia coli ATCC 25922 as the quality control. Second-generation sequencing analysis was conducted, followed by a comprehensive screening of antibiotic resistance genes using the Comprehensive Antibiotic Resistance Database database (Resistance Gene Identifier module). Results:Antimicrobial susceptibility was interpreted according to the Clinical and Laboratory Standards Institute guidelines. All 13 isolates demonstrated complete resistance (13/13, 100.00%) to β-lactams (meropenem, aztreonam, cefazolin, and ampicillin) and sulfonamides (sulfamethoxazole and trimethoprim). In contrast, all isolates were susceptible to fosfomycin and rifampicin (13/13, 100%). Variable susceptibility to florfenicol was observed (9/13, 69.23%). Genomic analysis revealed the spread of resistance genes (mcr-3.16, cphA5, bla OXA-956) in A. salmonicida. The key mechanisms include enzymatic inactivation and efflux pumps. Biocide resistance genes (qacEdelta1) indicate disinfectant co-selection. No explicitly novel genes were reported; however, emerging variants (e.g., bla OXA-956) were identified. Conclusion:This study provides the first comprehensive antimicrobial resistance profile of porcine-associated A. salmonicida. Our results demonstrated that these bacteria were resistant to almost all commonly used antibiotics. The genes conferring this extensive resistance were located on mobile genetic elements, meaning they can spread to other bacteria, which poses a significant threat to both animal and public health. To address this, we recommend the following: (1) implementing antibiotic susceptibility testing in veterinary practice to guide treatment choices and avoid ineffective drugs; (2) prioritizing prevention through improved farm hygiene and biosecurity to reduce infection rates; and (3) establishing coordinated surveillance across human, animal, and environmental sectors to monitor the spread of these resistant bacteria.

The excessive use of synthetic preservatives poses significant threats to food safety and human health. This study systematically investigated the genetic characteristics of the Limosilactobacillus fermentum (L. fermentum) z-6 strain, the antibacterial properties of the bacteriocin-like substance (FC) it produces, and its mechanism of action. The results demonstrated that this bacteriocin-like substance exhibited remarkable antimicrobial activity and exceptional stability, maintaining high activity across a broad pH range (4.0–8.0) and withstanding heat treatment at 100 °C and UV irradiation, indicating robust environmental adaptability. Its proteinaceous nature was confirmed by its detection below 1 kDa on Tricine-SDS-PAGE and its inactivation by trypsin and pepsin. The FC showed broad-spectrum inhibition against foodborne pathogens, including Salmonella enterica serovar Typhimurium, E. coli, A. baumannii, S. aureus, P. mirabilis and L. monocytogenes. Mechanistic investigation demonstrated that the FC exerts antibacterial effects primarily through membrane disruption, as evidenced by a live-dead staining assay confirming significantly enhanced permeability in Salmonella enterica serovar Typhimurium, and scanning electron microscopy revealing distinct pore formation on bacterial surfaces. It is speculated that the FC produced by z-6, due to its excellent properties and outstanding antibacterial performance, could potentially serve as a natural biopreservative.

Salmonella Typhimurium is a pathogen bacterium responsible for foodborne diseases. In this study, Weissella sagaensis ZD-66, a bacteriocin-producing strain, was isolated from pickled vegetables. The bacteriocin produced by this strain was characterized, and its antibacterial mechanism was investigated. The genome size of W. sagaensis ZD-66 was 1,862,304 bp, with a GC content of 36.87%. Further, bioinformatic analysis using the BAGEL4 database revealed a class IIa bacteriocin gene cluster, identified as Penocin A. W. sagaensis ZD-66 showed maximum antibacterial activity at 28 h during fermentation. The W. sagaensis ZD-66 was partially purified by using dialysis and Tricine-SDS-PAGE, which revealed a molecular weight of less than 1 kDa. The W. sagaensis ZD-66 showed broad-spectrum inhibition against foodborne pathogens, including S. Typhimurium, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, and Cronobacter sakazakii. The bacteriocin was stable at 121°C for 30 min, pH resistant (pH 3-7), and under UV exposure for up to 2.5 h. However, its antibacterial activity of bacteriocin decreases after treatment with pepsin, trypsin, and pronase E. Furthermore, the treatment with bacteriocin led to the cell membrane being damaged, nucleic acid leakage, and reduced intracellular ATP content. Observation of the ultrastructure of bacteria by Scanning electron microscopy revealed that bacteriocin can inhibit S. Typhimurium by disrupting the cell membrane and pore formation. These results suggest that the bacteriocin produced by W. sagaensis ZD-66 has the potential for a natural food preservative.

Objective: P. mirabilis is a Gram-negative rod-shaped bacterium. In this study, the SH-8 strain of P. mirabilis was isolated from swine-derived samples, and its antibiotic resistance genes (ARGs) and transmission mechanisms were systematically characterized. Methods: The minimum inhibitory concentrations (MICs) of strain SH-8 were determined by the broth microdilution method, with genomic features analyzed through whole-genome sequencing (WGS). Results: P. mirabilis SH-8 exhibited resistance to multiple antimicrobial agents. Its genome consists of a single chromosome (3,993,987 bp; GC content 38.8%) and one plasmid. WGS identified 21 distinct ARGs in this strain, along with a class 2/1 hybrid integron. Furthermore, a heatmap analysis of resistance genes from 29 P. mirabilis isolates of diverse origins revealed a striking similarity between the resistance gene profile of SH-8 and those of other strains. Conclusions: The MDR exhibited by P. mirabilis SH-8 is primarily attributed to integron-mediated resistance gene clusters within its genome, which may pose challenges for treating opportunistic infections caused by this bacterium.

IMPORTANCE:Kluyvera intermedia (K. intermedia) is an emerging pathogen associated with respiratory disease in pigs. Its multidrug resistance poses a significant threat to livestock health. OBJECTIVE:This study characterized the resistance phenotype and genotype of K. intermedia L-40-1, isolated from a pig in Jilin Province, China, and investigated the genomic determinants of its antibiotic resistance. METHODS:16S rRNA sequencing was performed to identify the strain. The resistance phenotype was assessed by determining minimum inhibitory concentrations (MICs), and resistance determinants were characterized by whole-genome sequencing. Comparative genomic analysis against the national center for biotechnology information (NCBI) database of K. intermedia strains was conducted to determine phylogenetic relationships and resistance gene profiles. RESULTS:L-40-1 exhibited high resistance to β-lactams, cephalosporins, macrolides, aminoglycosides, and sulfonamides. Comparative analysis identified 19 high-frequency resistance genes (e.g., arnT, rsmA, emrR, and acrA). A 159,618 bp plasmid (PYL-8) harbored multiple resistance genes (e.g., sul1, dfrA27, qnrB6, and tet(D)) and transposons (e.g., IS6, IS1, and Tn3), contributing to its multidrug resistance. Chromosomal and plasmid-borne resistance mechanisms were identified. CONCLUSIONS AND RELEVANCE:K. intermedia L-40-1 uses a combination of chromosomal and plasmid-mediated resistance genes, facilitated by transposons, to confer multidrug resistance. This highlights the need for surveillance of K. intermedia in livestock to mitigate the spread of antibiotic resistance.

为了研究吉林地区不同患病部位牛源化脓隐秘杆菌(Trueperella pyogenes)耐药表型存在差异的原因,阐明化脓隐秘杆菌的遗传进化关系,将从膝关节分离得到的化脓隐秘杆菌命名为TP2,并通过PacBio RS II技术对菌株TP2 的全基因组进行测序.将菌株TP2 进行药敏试验并与前期研究结果进行比对,发现菌株 TP1 和菌株 TP2 的耐药表型有差异.将菌株TP2 的全基因组测序结果与ARDB数据库比对,发现菌株TP2 仅有 1 个抗生素抗性基因,而前期研究中菌株TP1 有 6 个抗生素抗性基因.将菌株TP2 与前期研究中菌株TP1 的全基因组进行系统进化分析和共线性分析,发现菌株 TP1 和菌株 TP2 的亲缘性最显著.将菌株TP2 与前期研究中菌株TP1 的全基因组进行Ka/Ks分析,发现菌株TP1 和菌株TP2 存在部分基因Ka/Ks值大于 1,基因受正选择.以上研究结果为化脓隐秘杆菌基因的遗传进化和耐药机制的研究提供参考,为预防和治疗化脓隐秘杆菌病提供了理论基础.

拟态弧菌(Vibrio mimicus,V.mimicus)是一种人与水生动物共患病原菌,该菌不仅可以感染黄颡鱼、南方鲇等经济鱼类,对水产养殖业造成严重经济损失,同时会对食品安全和公共卫生等方面造成严重的潜在威胁.研究人员在传统细菌鉴定方法的基础上加以改进,同时利用分子生物学技术研发出了更高效、特异性更强的鉴定方法.拟态弧菌目前被发现的毒力因子主要有外膜蛋白(outer membrane protein,OMP)、外毒素以及菌毛等,主要引起感染鱼类的皮肤溃疡出血以及腹水病,人类食用会引发腹泻等不良反应.由于抗生素的长期不合理应用,近年来国内外均出现了拟态弧菌对于β-内酰胺类抗生素、磺胺类抗菌药物等的耐药现象.该文介绍了拟态弧菌的鉴定方法、致病性、耐药性及其防治技术,以期为拟态弧菌病的有效防控提供有益参考.

针对猪圆环病毒-Ⅱ型(porcine circovirus type Ⅱ,PCV-Ⅱ)、猪瘟病毒(swine fever virus,CSFV)和猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)的保守区域设计用 于构建荧光定量PCR体系的引物和探针.以构建的重组质粒标准品为模板建立三重荧光定量PCR体系,并对三重荧光定量PCR体系进行优化和特异性检测、灵敏性检测、重复性试验、标准曲线制作.最后利用构建的三重荧光定量PCR体系和普通PCR体系同时进行临床检测,并对两者的检测结果进行对比.结果显示,3种病毒在单重和三重荧光定量PCR体系中均无非特异性扩增;3种病毒在单重和三重荧光定量PCR体系中的灵敏度均可达到10拷贝/μL;单重和三重荧光定量PCR体系组内和组间重复性变异系数均在5％以内;单重和三重荧光定量PCR在稀释度范围内呈现良好的线性关系.应用本研究构建的三重荧光定量PCR体系对106份临床组织样品进行检测,其中PCV-Ⅱ阳性检出率为74.5％,CSFV阳性检出率为34.9％,PRRSV阳性检出率为18.9％,普通PCR检测中PCV-Ⅱ阳性检出率为37.7％,CSFV阳性检出率为16.9％,PRRSV阳性检出率为7.5％.

针对猪细小(PPV)、猪伪狂犬(PRV)和猪繁殖与呼吸综合征(PRRSV)病毒的保守区域设计用于构建荧光定量PCR体系的引物和探针.以构建的重组质粒标准品为模板建立三重荧光定量PCR体系,并对三重荧光定量PCR体系进行优化并进行特异性、灵敏性、重复性等试验.最后利用构建的三重荧光定量PCR体系和普通PCR体系分别检测病料以进行比较.结果表明:三种病毒在单重和三重荧光定量PCR体系中均无非特异性扩增;三种病毒在单重和三重荧光定量PCR体系中的灵敏度均可达到10 copies/μL;单重和三重荧光定量PCR体系组内和组间重复性变异系数均在5％以内;单重和三重荧光定量PCR在稀释度范围内呈现良好的线性关系.应用本研究构建的三重荧光定量PCR体系对164份临床组织样品进行检测,其中PPV阳性检出率为16.7％,PRV阳性检出率为10％,PRRSV阳性检出率为18.9％;普通PCR检测中PPV阳性检出率为6.5％,PRV阳性检出率为3.6％,PRRSV阳性检出率为7.5％.说明本研究建立的PPV、PRV和PRRSV三重荧光定量PCR检测方法符合临床检测要求并且比普通PCR方法更敏感、更特异.

化脓隐秘杆菌(Trueperellapyogen)是一种重要的条件致病菌,能够引起猪、牛、羊和其他动物的化脓性感染.临床常采用抗菌药物治疗由化脓隐秘杆菌引起的感染,但研究发现,近年来多地已经发现化脓隐秘杆菌对β-内酰胺类、大环内酯类、四环素类药物都出现不同程度的耐药现象.因此,了解其耐药机制对研究该细菌病的替抗药物以及新型治疗方法具有实际意义.论文就化脓隐秘杆菌对6类抗菌药物的耐药表型以及耐药机制进行了总结,以期为临床用药和抗菌药物的研发提供参考.

为了确定吉林市某养殖场山羊患病的病因,本试验采集患病山羊的鼻拭子样品并进行实验室培养,从中分离到1株莫拉菌,命名为MC-1.通过对MC-1的细菌形态观察、生化鉴定、16S rDNA序列测定和系统进化树分析,确定该菌株为山羊莫拉菌(Moraxella caprae).药敏试验结果显示,该菌株对阿莫西林、四环素、阿奇霉素、青霉素、头孢曲松、庆大霉素等多种抗菌药物均敏感.小鼠致病性试验结果显示,该菌株毒性较弱.本试验为深入探讨山羊莫拉菌的病理机制与防控手段提供了科学依据.

校企合作、产教融合是应用型大学建设的必由之路,"双师型"教师队伍是应用型人才培养的基本保障,如何加强"双师型"教师的培养是高水平应用型大学面临的关键问题.该文以吉林农业科技学院动物医学专业"双师型"教师队伍为例,从专业"双证"准入制、"产、教、学"融合实践能力提升、校企项目合作和培训平台建设促进理论教学能力提高等方面探究了动物医学专业"双师型"教师队伍建设的路径,虽不甚全面,旨在提供参考.

Porcine reproductive and respiratory syndrome virus (PRRSV) causes a highly contagious disease and brings huge economic losses to commercial pork production worldwide. PRRSV causes severe reproductive failure in sows and respiratory distress in piglets. To trace the evolution of PRRSV in pigs with respiratory diseases in some regions of China, 112 samples were collected from nine provinces in China during 2016–2018. All samples were detected by RT-PCR and analyzed by the Nsp2/ORF5 (ORF5a)-genes-phylogeny. Sequence analysis and recombination analysis were conducted on the Nsp2/ORF5 (ORF5a) genes of the identified strain in the study. The RT-PCR result shown that the positive rate of PRRSV was 50.89% (57/112). Phylogenetic analysis showed that the identified PRRSV strains were all NA genotype and belonged to lineage 1, 3, and 8. The Nsp2 gene of identified PRRSV strains exhibited nucleotide homologies of 53.0 ~ 99.8%, and amino acid homologies of 46.8 ~ 99.7%. The ORF5 gene of identified PRRSV strains exhibited nucleotide homologies of 82.4 ~ 100%, and amino acid homologies of 79.6 ~ 100%. Sequence analysis revealed that a discontinuous 30-amino-acid deletion (positions 481 and 533–561) and a 131-amino-acid discontinuity deletion (positions 323–433, 481, and 533–551) in Nsp2 of PPRSV isolates; all identified strains in this study may be wild strains, and most identified strains may be highly virulent strains. Sequence analysis of ORF5 and ORF5a revealed that the mutation sites of GP5 were mainly concentrated in the signal peptide and epitopes region, while the mutation sites of ORF5a were mainly concentrated in the transmembrane and the intramembrane region. The recombination analysis indicated that there may be multiple recombination regions in identified strains, and the recombination pattern was more complex. This study showed that the prevalent PRRSV strain in some regions of China was still HP-PRRSV, while NADC30 strain also occupied a certain proportion; different types of PRRSV strains showed different patterns and variation in China. This study suggested that the monitoring of PRRSV prevalence and genetic variation should be further strengthened.

为提升分子生物学在本科学生中的关注度,推动学生创新及加强基础实践能力,该文使用创新研究方法,即全方面利用当前大数据教学资源,由专业教师团队旁站指导,分配学生成立课题小组,让其发挥自身主观能动性寻找、收集匹配自身已积累知识的相关材料,并进行组内讨论及分析.结果显示:采用此教学模式,学生的知识主动吸纳度、对所得材料的分析及总结能力都有较大提高;学生的学习主观能动性(知识汲取、情报收集、分析、汇总)也得到了提高,并且促进了学生彼此之间分享知识的意愿,提高了学生学习的兴趣.

为了解吉林地区猪细环病毒(TTSuVs)与Ⅱ型猪圆环病毒(PCV2)混合感染情况,分析TTSuVs感染与仔猪多系统衰竭征(PMWS)的相关性.试验通过PCR技术对2019年收集到的吉林部分地区的10家规模化猪场130份血清样本进行检测;并应用本实验已建立的PCV2、TTSuVs实时荧光定量PCR检测方法,对PCV2阳性样品进行病毒载量的分析,区分PMWS病猪与PCV2亚临床感染猪,比较PMWS病猪与PCV2亚临床感染猪中TTSuVs的载量.结果显示:TTSuV1感染率为38.46％;TTSuV2感染率为57.69％;PCV2感染率为46.15％;TTSuV1与TTSuV2的混合感染率为31.54％;TTSuV1与PCV2的混合感染率为32.30％;TTSuV2与PCV2的混合感染率为44.62％;三种病毒的混合感染率为22.30％.且PMWS病猪血清中TTSuV2载量明显高于PCV2亚临床感染猪(P＜0.01).结果表明,TTSuV2载量与PMWS病的发生存在一定程度的相关性.