Malignant meningiomas often show invasive growth that makes complete tumor resection challenging, and they are more prone to recur after radical resection. Invasive meningioma associated transcript 1 (IMAT1) is a long noncoding RNA located on Homo sapiens chromosome 17 that was identified by our team based on absolute expression differences in invasive and non-invasive meningiomas. Our studies indicated that IMAT1 was highly expressed in invasive meningiomas compared with non-invasive meningiomas. In vitro studies showed that IMAT1 promoted meningioma cell invasion through the inactivation of the Krüppel-like factor 4 (KLF4)/hsa-miR22-3p/Snai1 pathway by acting as a sponge for hsa-miR22-3p, and IMAT1 knockdown effectively restored the tumor suppressive properties of KLF4 by preserving its tumor suppressor pathway. In vivo experiments confirmed that IMAT1 silencing could significantly inhibit the growth of subcutaneous tumors and prolong the survival period of tumor-bearing mice. Our findings demonstrated that the high expression of IMAT1 is the inherent reason for the loss of the tumor suppressive properties of KLF4 during meningioma progression. Therefore, we believe that IMAT1 may be a potential biological marker and treatment target for meningiomas.

目的 探讨中性粒细胞/淋巴细胞比值(NLR)和血小板/淋巴细胞比值(PLR)与高脂血症(HLP)的相关性.方法 回顾性分析495例HLP患者(HLP组)和500名健康体检者(对照组)的实验室检测结果 .结果HLP组NLR和PLR明显高于对照组(P=0.007).单因素分析结果显示,白细胞、血红蛋白水平、NLR、PLR与HLP呈中度相关.受试者工作特征(ROC)曲线分析发现,NLR的最佳临界值为1.71(P<0.001),敏感性为68.0％,特异性为78.5％,NLR的预测强度优于其他指标,且HLP的严重程度与NLR呈高度正相关.结论 HLP患者的NLR和PLR明显高于健康体检者,NLR随HLP严重程度相应上升.

多发性内分泌腺瘤病(multiple endocrine neoplasia,MEN)是一组有明显家族倾向的常染色体显性遗传性疾病,表现为在同一患者身上同时或先后出现2个及以上的内分泌腺肿瘤或因增生而产生的一种以受累腺体功能亢进为表现的临床综合征.MEN可分为MEN 1型及MEN 2型,后者又可分为MEN 2A型、MEN 2B型.此外,还有不能归属于MEN 1或MEN 2的混合型MEN[1],此类疾病临床少见.复旦大学附属华山医院因降钙素异常增高发现多发性内分泌腺瘤病2A型1例.

Objective To evaluate the role of complement (C) 1 q determination for autoimmune diseases (AID).Methods A total of 203 AID patients [86 cases of autoimmune thyroid disease (AITD),56 cases of systemic lupus erythematosus (SLE),32 cases of rheumatoid arthritis (RA) and 29 cases of other connective tissue diseases]and 100 healthy subjects (healthy control group) were enrolled from the Second Affiliated Hospital of Nanchang University from May 2014 to September 2015.Using transmission turbidimetry,turbidimetric immunoassay,indirect immunofluorescence (IIF) and linear immunoblot assay (LIA),C1q,C-reactive protein (CRP),antinuclear antibody (ANA),anti-double-stranded DNA (ds-DNA) antibody and anti-Sm antibody were determined respectively.Results Compared with healthy control group,C1q level in SLE group was decreased (P＜0.05),and that in RA group was increased (P＜0.05).The level of C 1 q decreased with the increasing of ANA titer degrees (P＜0.05).The C1q levels in anti-ds-DNA antibody positive and anti-Sm antibody positive group were lower than those in anti-ds-DNA antibody negative and anti-Sm antibody negative group (P＜0.05).CRP levels in AID groups were increased (P＜0.01).The correlation coefficient between C1q and CRP was ＞0.300 (P＜0.05).Regression equation showed that the range of cut-off values (COV) was 167.42-198.37 mg/L.For C1q and autoantibodies,the abnormal rate (C1q＜COV) was higher in ANA positive group than that in ANA negative group,that in ANA positive with anti-ds-DNA positive group was higher than those of other autoantibody distribution types,and that in ANA positive with anti-Sm antibody positive or with anti-Sm and anti-ds-DNA antibodies positive increased.The abnormal rate (C1q＞COV) in ANA negative group was 67.2％.Conclusions For patients with AID,C1q has moderate correlation with CRP,the abnormal rate of C1q has correlations with some autoantibody distribution types and the status of patients' illness.

目的：探讨补体C1q检测对自身免疫性疾病（AID）的诊断及临床价值。方法对南昌大学第二附属医院2014年5月至2015年8月符合纳入标准的156例AID患者及40例健康体检者作为研究对象，采用透射比浊法检测其补体C1q、核酸荧光染色技术（DIFF通道）和激光流式分析技术（WBC/BASO通道）双方法检测白细胞总数（WBC）、免疫比浊法检测C反应蛋白（CRP）及改良魏氏法检测血沉（ESR），并对结果进行回顾性分析。结果与健康对照组相比，补体C1q在系统性红斑狼疮（SLE）组平均水平下降[(171.58±37.22 vs 192.08±27.85)mg/L，P＜0.05]，补体C1q在其他AID升高，其中类风湿关节炎（RA）与对照组相比[(230.17±65.29 vs 192.08±27.85)mg/L，P＜0.05]；WBC水平在AID中有所升高，但敏感性不强；CRP、ESR在AID中均明显升高(P＜0.05)，补体C1q、CRP和ESR之间的相关系数均大于0.3（P＜0.01），呈中度相关；自身免疫性甲状腺疾病（AITD）、SLE、RA、其他结缔组织疾病患者补体C1q不在正常范围内的总阳性率分别为44.23%、60.00%、73.33%、51.72%，其低值阳性率分别为9.62%、53.33%、16.67%、13.79%，其高值阳性率分别为34.62%、6.67%、56.67%、37.93%。结论补体C1q水平的变化及CRP、ESR的升高对AID患者的临床诊断具有一定的指导意义，补体C1q、CRP及ESR之间存在一定的相关性，提示补体C1q是一个炎症反应指标，联合检测对临床的诊断具有一定的指导作用。

Objective To explore diagnostic significance and clinical evaluation of the antinuclear antibody (ANA) and anti-centromere antibody (ACA) detection in patients with small cell lung cancer (SCLC). Methods 66 cases of SCLC patients,116 cases of patients with other tumors and 40 cases of healthy people of serum as the research objects who come from Xin Jianxian Traditional Chinese Medicine Hospital and the Second Affiliated Hospital of Nanchang University from January 2015 to May 2016. The result of ANA and ACA was determined using human laryngeal epithelial cells-20-10 (HEp-20-10) and human laryngeal ep-ithelial cells-2 (HEp-2) as the matrix by indirect immunofluorescence (IIF),and the results were retrospectively analyzed. Results Compared with non-small cell lung cancer (NSCLC),ANA in serum positive rate of SCLC significantly increased (P<0.01);ANA of SCLC patients was compared with liver cancer patients,there were no statistically significant difference(P>0.01);SCLC compared with the cases in the control group,the ACA detection rate were significantly increased,the differences were statistically significant (P<0.01);Each group of ANA in serum and lung cancer detection rate of ACA in comparison with healthy controls were signifi-cantly higher(P<0.01);The HEp-10-20 and HEp-2 as the matrix of the IIF method to test the serum ACA respectively ,there were no statistically significant differences (P=0.824) to SCLC patients,Kappa consistency analysis,P<0.01,there is a significant differ-ence,but the Kappa=0.333,poor consistency;Combined two different substrate detection in patients with SCLC results showed that the positive rate significantly increased (P<0.05),but from the clinical evaluation indexes: matrix for HEp-20-10 IIF the clinical significance of the detection results are more significant. Conclusion The diagnosis and differential diagnosis of ACA detection in patients with SCLC has important clinical significance,using HEp-20-10 cell lines antibody detection of ACA will be having a better deterministic rate in patients with SCLC.

Objective To analyze the sensitivity,specificity and coincidence rate of three methods for detecting anti-double stranded DNA (anti-dsDNA) antibody,and to explore the correlation of anti-dsDNA results and activity of systemic lupus erythe-matosus (SLE). Methods Anti-dsDNA antibody from 640 cases of inpatient and healthy controls,including 215 cases of SLE were measured by indirect immunofluorescence (IIF),linear imprinting assay (LIA) and radioimmunoassay (RIA). Then the data were an-alyzed using retrospective method. Results The detectable rates of anti-dsDNA antibody in SLE by IIF,LIA and RIA were 40. 00%,43.72%and 47.44% respectively,with significant differences against the control group. The specificity of the three methods were 98.35%,96.24% and 94.59% respectively,in which the coincidence rates were 78.75%,78.59% and 78.75% respectively (kappa>0.8). Of all the active SLE case diagnosed by the methods of IIF,LIA and RIA the detectable rates were 60.76%,58.23%and 60.76% respectively,which significantly differentiate stable SLE from active SLE. Moreover,the concentration of anti-dsDNA antibody by RIA and the activity of SLE were found to exist in an outstanding linear trend (P<0.05). Conclusion Anti-dsDNA antibody played an important role in the diagnosis and active evaluation of SLE,which suggested the combination usage of two or more methods if possible.

目的：报道1例抗核抗体(ANA)初筛假阴性，而经多滴度稀释核型显现阳性且呈现不同种类核型的系统性红斑狼疮(SLE)的临床特点、诊断及预后。方法采用间接免疫荧光法(IIF)检测ANA。结果患者血清在1：100初筛滴度下显示ANA假阴性，但不同滴度下显现ANA均质型、颗粒型、核仁型及抗细胞浆抗体。结论行ANA检测时，对胞浆与胞核均呈现荧光，易被判断为非特异性荧光而误判为阴性的情况，做不同滴度稀释，并结合ANA谱结果发出报告，以免漏诊延误病情。