Objective To reveal the patho-physiological role of urotensin Ⅱ(UⅡ),a new vasoconstriction peptide,in the occurrence and development of hypoxic pulmonary hypertension(HPH),and to investigate the effects of hypoxia on UⅡ gene expression,synthesis and release in various cell components of pulmonary tissues.Methods HPH rat models were established using immunohistochemistry(IHC) to observe the distribution of UⅡ on various cell components of rat pulmonary tissues at different time points of hypoxia.Results UⅡ was widely distributed in a variety of pulmonary cells.Its expression was boosted in various cells of pulmonary tissues after appearance of hypoxia,especially in hypoxia 10 d and 20 d.Conclusion Hypoxia can promote many lung tissue cells to synthesize and excrete UⅡ,and lung tissue UⅡ to release,too.UⅡ participates in the occurrence and development of HPH.

Objective To study the content changes of plasma urotensin Ⅱ(U Ⅱ)and adrenomedullin(ADM)in patients with chronic obstructive pulmonary disease(COPD)at different stages of disease so as to investigate the impact of hypoxia on synthesis and release of U Ⅱ,and the relationship between U Ⅱ and ADM and its significance,with a purpose of opening a new thinking way of pathogenesis of COPD and providing a new strategy for prevention and treatment of COPD.Methods The radioimmunoassay(RIA)was taken to detect the contents of plasma U Ⅱ and ADM.The changes of SaO2 and pulmonary arterial pressure(PAP)were also monitored.Results There were significant differences in PAP,SaO2 the contents of U Ⅱ and ADM of the COPD patients among the three groups(P<0.01).In COPD patients U Ⅱ content was negatively correlated with SaO2(P<0.05),and positively correlated with PAP(P<0.05).In patients with COPD UⅡcontent was significantly increased,and higher at acute exacerbation stage than at remission stage,and the content of U Ⅱ and ADM were of a good positive correlation.Conclusion U Ⅱ as a vasoconstrictor factor may be involved in the formation of hypoxic pulmonary hypertension,and the increase of plasma U Ⅱ level followed by increased ADM in the body is one of the self-protective mechanisms.

目的:探讨低氧性肺动脉高压大鼠肺组织中尾加压素Ⅱ的合成、分泌及其在低氧性肺动脉高压病理生理过程中的作用。方法:实验选用雄性Wistar大鼠76只,随机分为正常对照组,低氧10,20,30d组,每组19只,在低氧10d和20d后各有1只死亡。间断常压低氧方法复制低氧性肺动脉高压大鼠模型,应用光镜、免疫组化、放射免疫分析等方法,测定低氧各组和对照组大鼠肺组织中尾加压素Ⅱ蛋白表达及血浆、支气管肺泡灌洗液尾加压素Ⅱ含量的动态变化。结果:各组大鼠支气管黏膜上皮细胞、肺血管内皮细胞及其平滑肌细胞、肺巨噬细胞、肺泡Ⅱ型上皮细胞和支气管软骨细胞中尾加压素Ⅱ均呈阳性表达,低氧后上述各种细胞中尾加压素Ⅱ表达明显增强。血浆和支气管肺泡灌洗液尾加压素Ⅱ浓度于低氧10d开始升高（4.26±1.61）,（20.93±6.64）pmol/L,20d时两者均达高峰（9.03±1.80）,（34.71±5.31）pmol/L明显高于对照组（3.74±1.08）,（16.83±4.99）pmol/L（t=6.15,7.39,P＜0.001）,低氧30d出现下降趋势,但仍高于对照组（t=2.24,3.97,P＜0.05）。结论:低氧可促进肺组织中尾加压素Ⅱ的合成和释放;在低氧性肺动脉高压病理生理过程中,尾加压素Ⅱ对调节肺循环和肺通气及肺血管改建等方面发挥重要作用。

Objective:To investigate the potential impact of SARS virus on immunological function in medical personnel who had close contact with SARS patients and further to provide theoretical basis for prevention and treatment of the disease.Methods:Colloidal gold assay (CGA) and ELISA were adopted to detect antibody IgG against SARS virus in SARS patients and the medical personnel who had kept close contact with these patients at a SARS special hospital as well as a non-special SARS hospital.Moreover,Special Protein Analyzer was used to measure levels of C-reactive protein (CRP),complement component 3 and 4 (C3,C4) and circulating immune complex (CIC) in the SARS patients and the medical personnel.Thirteen normal subjects were selected as controls to undergo the same tests.Results:In controls,no specific IgG antibody against SARS virus was detected;in the medical personnel from the non-special hospital,specific antibody against SARS virus was found in one person by CGA and one by ELISA with a positivity of 1.14%.The 11 patients with SARS were all positive for specific antibodies against SARS virus and the positivity was 100%.The CRP level in the SARS patients was as much as 3.1 times,3.5 times and 2.84 time higher than in controls,medical personnel from the non-special hospital and from the the special SARS hospital (Xiaotangshan Hospital) with a significant difference (P0.01).Compared with the controls,the concentrations of C3 and C4 in medical personnel from the non-special hospital were decreased by 25.2% and 27.59%,respectively,while C3 dropped by 13.39% in the personnel from Xiaotangshan Hospital (P0.05).When compared with those in the personnel from Xiaotangshan,C3 and C4 were reduced by 13.64% and 22.22% respectively;there was a significant difference between the two groups of medical personnel;CIC concentration was increased by 20.02% (P0.05).Conclusions:Silent SARS virus infection might be present.CRP was related with the clinical symptoms of the infection and it might serve as a indicator for monitoring the SARS patient's condition.The results of immunological function in the medical personnel shown that immunoreaction in those from Xiaotangshan Hospital was slighter than from the general hospital,indicating that Xiaotangshan Hospital was very well equipped with a isolation and protective system.

<span id="ChDivSummary" name="ChDivSummary" class="abstract-text">目的　分析SARS流行期间小汤山医疗队医务人员机体免疫水平 ,评价小汤山医院SARS防护措施。方法　采用流式细胞术和血细胞分析仪分别检测小汤山医疗队医务人员、SARS流行期间北京驻军某部健康官兵的外周血白细胞总数、淋巴细胞群CD3 T淋巴细胞 （包括CD4、CD8细胞 ）、CD+ 56NK细胞、CD+ 19B细胞绝对数 ,并与SARS流行前健康人群正常值比较。结果　小汤山一线医务人员和SARS流行期间健康官兵的白细胞总数、淋巴细胞及其亚群CD+ 3 T淋巴细胞、CD+ 4 细胞、CD+ 8细胞、CD+ 19B细胞绝对数均降低 ,而CD+ 56NK细胞升高 ,与SARS流行前健康人群正常值比较 ,有显著性差异 （P &lt;0 0 0 1,P &lt;0 0 5 ） ,一线医务人员与同期健康官兵比较无显著性差异。结论　SARS流行后医务人员的免疫功能降低主要与心理应激有关 ;采取隔离防护措施可有效保护应激状态下医务人员的安全。</span>

Objective To observe the dynamic changes of urotensionⅡ (U Ⅱ) content in plasma and bronchoalveolar lavage fluid (BALF) of rats with chronic hypoxic pulmonary hypertension and to investigate the effects of hypoxia on the synthesis and secretion of U Ⅱ and the correlation of U Ⅱ with the mean pulmonary artery pressure (mPAP) and the partial pressure of arterial oxygen (PaO 2). Methods Male Wistar rats were randomly divided into control group and hypoxic groups. Model of chronic hypoxic pulmonary hypertension was established by normal barometric and discontinuous hypoxia. mPAP and RVdp/dt max of each rat were measured using right cardiac catheterization. PaO 2 was detected by blood gas analysis. U Ⅱcontents in plasma and BALF were detected by radioimmunoassay. Results During hypoxia, mPAP, RVdp/dt max , U Ⅱ content in plasma, and BALF increased in rats (P0.01), and peaked at 20 d after hypoxia, but fell at 30 d after hypoxia. PaO 2 decreased during hypoxia (P0.05). There was a positive correlation between U Ⅱ content in plasma and mPAP in hypoxic groups (r=0.614, P0.01 ). There was a negative correlation between UⅡcontent in plasma and PaO 2 in hypoxic groups (r=-0.620 , P0.01). The U Ⅱ content in BALF was higher than that in plasma (P0.01). There was a correlation between plasma and BALF about UⅡ content (r=0.820, P0.01). Conclusion Hypoxia may result in the elevation of UⅡ in rats. UⅡ, not only an autocrine and /or paracrine factor but also a circulating hormone, may play an important pathophysiological role in the genesis of hypoxic pulmonary hypertension.

鼻腔上颌窦腺鳞癌一例黄友苗天生范平彭丽荣刘伯英赵鹏一种癌出现鳞及腺的分化即腺鳞癌，可见于子宫、宫颈、食管、支气管和肺等，发生于鼻腔、鼻窦者罕见，我院收治１例，报道如下。患者女，６８岁。左侧进行性鼻阻３个月，伴头痛、左眼溢泪、左耳鸣、听力下降１月于１９...