Objective To explore the clinical application value of the levels of serum miRNA-146,miRNA-195 in diabetic retinopa-thy. Methods The plasma and clinical data from 91 cases of the patients with DR were collected,38 cases of volunteer blood plasma from a ran-dom sample of the general population were extracted. The Expression levels of miRNA-146,miRNA-195 were quantitatively detected by RT-PCR method. The patients with diabetic retinopathy were divided into the non-proliferating diabetic retinopathy group (NPDR)and proliferating diabetic retinopathy group (PDR). The relationship between the changes of the expression of the levels of the serum miRNA-146,miRNA-195 and DR were observed. Results In the case group,the levels of serum miRNA-146,miRNA-195 raised and lowered respectively,compared with that in control group with significant difference (P < 0. 01). The expression levels of miRNA-146 in NPDR group were lower than that in PDR group (P < 0. 01). The expression of the miRNA-195 were higher than that in PDR group (P < 0. 01). The levels of miRNA-146, miRNA-195 were negatively correlated in both NPDR and PDR group (r = 0. 687,-0. 692,P < 0. 01). The expression level of miRNA-146 and miRNA-195 respectively had correlation with the levels of glycosylated hemoglobin (HbAlc),urine white silkworm/ creatinine and fasting plasma glucose (FPG)in NPDR and PDR group (r = 0. 532,0. 610,0. 587,P < 0. 01;r =-0. 538,-0. 62,-0. 56,P < 0. 01). Conclu-tion Detection of the expression of serum miRNA-146,miRNA-195 can predict the occurrence of DR and help to determine its dangerous de-gree and estimate the development of the disease.

目的：探讨不同孕期和孕妇年龄对机体甲状腺激素水平的影响。方法：选择375例无甲状腺疾病史的孕妇，并以80例健康体检的非妊娠女性作为对照组，比较对照组和不同孕期、不同年龄的孕妇血清总三碘甲状腺原氨酸（T T 3）、总甲状腺素（T T 4）、游离三碘甲状腺原氨酸（FT 3）、游离甲状腺素（FT4）、促甲状腺激素（TSH）水平，以及抗甲状腺球蛋白抗体（TGAb）、抗甲状腺过氧化物酶抗体（TPOAb）阳性表达情况。结果：早孕期组、中孕期组和晚孕期组孕妇 T T3、T T 4、FT 3、FT 4、TSH 水平与对照组比较，差异均有统计学意义（P ＜0．05），早孕期组、中孕期组和晚孕期组FT 3、FT4水平依次降低（P＜0．05），中孕期组及晚孕期组 TSH 较早孕期组降低（P ＜0．05）；＜28岁组、28～34岁组和≥35岁组孕妇 T T3、T T 4、FT3及 FT4水平均依次降低，TSH 水平依次升高（P＜0．05），≥35岁组孕妇 FT3和 FT4水平较＜28岁组孕妇降低、TSH 水平升高（P＜0．05）；早孕期组、中孕期组和晚孕期组 TPOAb 阳性率及 TGAb 阳性率均依次降低（P ＜0．05）；TPOAb 阳性率为10．13％（38／375）。结论：妊娠期甲状腺激素水平与孕周及孕妇年龄密切相关，早孕期监测孕妇甲状腺激素水平对于甲状腺功能异常的检出及母儿健康具有重要意义。