宫颈癌是妇科常见的恶性肿瘤,近年来其发病年龄有年轻化的趋势.大量研究表明在宫颈癌的发生和发展过程中,除基因突变和高危型HPV持续感染等致病因素外,表观遗传调控也起到了重要作用.表观遗传是在基因组序列不改变的前提下,使DNA以外的其他可遗传信息在细胞增殖和发育过程中稳定传递.本文概述了DNA甲基化修饰、组蛋白修饰及非编码RNA调节等方式在中药防治宫颈癌中的作用,发现中医药对宫颈癌的治疗独具特色,是宫颈癌治疗中不可或缺的一部分.中药及中药复方成分复杂、作用靶点多样,能够通过表观遗传修饰中DNA甲基化,组蛋白修饰、非编码RNA调控等多种方式发挥抗肿瘤作用,降低肿瘤细胞浸润和转移.深入探讨"中药-宫颈癌-表观遗传学"之间的关系,研究中药干预表观遗传修饰在宫颈癌发生、发展过程中的作用,有利于宫颈癌的早期诊断和个体化治疗.

Oddi括约肌运动功能障碍是消化系统临床常见疾病之一,西医治疗副作用大,远期效果不理想,中医药对Oddi括约肌运动功能障碍有独到的认识和丰富的治疗经验.本文介绍了谢晶日教授治疗Oddi括约肌运动功能障碍的临证经验,治疗上谢晶日教授主张在中医特色辨证的基础之上进行脏腑分治,运用理气止痛以疏肝,调畅气机以健脾,祛瘀通络以利胆的治疗原则,且在临床上取得良好疗效.同时将中医内服治疗与穴位贴敷疗法相辅结合,并强调注重患者的情绪、心理及饮食调护,以巩固临床疗效.

Oddi括约肌运动功能障碍是消化系统临床常见疾病之一,主要由胆囊切除后所导致。中医学对Oddi括约肌运动功能障碍有独到的认识和丰富的治疗经验。笔者从循经选穴特点、中药特色贴敷、针贴结合特色、典型病例多角度介绍运用中医经典,针药结合治疗Oddi括约肌运动功能障碍的临床特色及经验。在Oddi括约肌运动功能障碍的治疗中应重辨证、识病因,以疏为本,以通为先,以健为辅,注重疏肝以调畅气机、利胆以通壅活络、健脾以扶土祛邪,并结合脉络同治,强调治疗持久持效之法。在针灸循经选穴治疗之后,结合特色中药穴位贴敷,辅以疏肝健脾利胆中药,着重应用原穴、背俞穴及八会穴以透腠理、充血脉、补脏腑,体现针药相结合的治疗特色,在临床上取得了较好的疗效。

Objective:To explore the influence of Berberine on testosterone level and the expressions of endometrial gene and its protein in rats with polycystic ovarian syndrome (PCOS).Methods:120 female Sprague-Dawley rats of 90 days were made into PCOS models with 22-day subcutaneous injection of insulin and human chorionic gonadotropin(HCG).After modeling,Berberine was given to rats in the next 28 days.The testosterone levels was tested by ELISA method;the protein expression of endometrial estrogen receptor α(Era) was detected with immunohistochemical method;the expression levels of endometrial Kras and PTEN mRNA gene were detected with qRT-PCR technique.Results:Compared to the model group,the testosterone level in Berberine group was significantly decreased(P<0.05);the positive protein expression of Era,and the gene expressions of Kras and PTEN mRNA were significantly reduced in endometrial tissue(P<0.05).Conclusion:Berberine has significant effects on the endometria of PCOS rats,which regulates the hormone synthesis and cell carcinoma.

Objective:To observe the clinical therapeutic effect of Erhuang Powder treating cervical intraepithelial neoplasia (CIN) ofⅠ-Ⅱ level.Methods:50 cases were randomly divided into the treatment group(Erhuang Powder group, 25 cases) and the control group(human recombinant interferon gel group, 25 cases).The clinical efficiency, the negative conversion rate of HPV, and the improvement of vagina discharge were compared after two menstrual cycles.Results: The clinical efficacy rate was 72.0% in the treatment group, which was higher than 44.0% in the control group, and there was a statistical significance between the two groups(P<0.05);in terms of the negative conversion rate of HPV, there was no difference between the two groups(P>0.05);The volume, colour and property of vagina discharge were significantly improved in the treatment group, which was superior to the control group (P<0.05).Conclusion:Erhuang Powder has a distinct effect on CIN of I-II level, with less adverse reactions, which is worthy of clinical application.

Objective To observe the clinical efficacy of thread-embedding at the Back-Shu and Front-Mu acupoints in treating senile constipation. Method Sixty-four eligible senile constipation patients were randomized into a treatment group and a control group,32 in each group. The treatment group was intervened by thread-embedding at the Back-Shu and Front-Mu acupoints,while the control group was by medication,8 weeks as a treatment course. The clinical efficacies of the two groups were evaluated. Result The total effective rate was 96.9% in the treatment group versus 84.4% in the control group,and the difference was statistically significant(P0.05). Conclusion Thread-embedding at the Back-Shu and Front-Mu acupoints is an effective approach in treating senile constipation.