Background: Systemic inflammatory biomarkers offer accessible and dynamic prognostic information in pancreatic ductal adenocarcinoma (PDAC). This study explores how circulating inflammatory indices enhance risk stratification and outcome prediction for PDAC patients undergoing radical resection. Methods: We analyzed 368 PDAC patients categorized by clinically relevant postoperative pancreatic fistula, resectability status, and Clavien-Dindo complication scores. Blood-based inflammatory indices were used to establish clinical risk profiles. Optimal cutoff values were determined using Youden's index. A prognostic nomogram was developed through multivariable regression and the least absolute shrinkage and selection operator (LASSO) regression analysis, and its predictive performance was evaluated using time dependent receiver operating characteristic (ROC) curves against conventional staging systems. Results: Patients with borderline resectable (BR) PDAC and those with severe postoperative complications showed significantly elevated inflammatory indices. Those with inflammatory markers above optimal cutoff points had significantly poorer survival. A nomogram incorporating C-reactive protein (CRP), cancer antigen 199 (CA19-9) >500 U/mL, radiological resectability, and tumor size demonstrated superior prognostic accuracy over traditional staging systems and improved clinical decision-making. Conclusions: Circulating inflammatory biomarkers significantly improve prognostic assessment in patients with PDAC and can reflect the risk of surgical complications. Our findings support incorporating accessible blood-based inflammatory indices into multimodal risk stratification frameworks to guide personalized treatment strategies.

PURPOSE:Choroidal neovascularization causes vision loss, necessitating the development of novel therapeutic methods. This study examines the role of eriocitrin, a flavonoid, in choroidal neovascularization and its potential mechanism of action. METHODS:A murine laser-induced choroidal neovascularization model and hypoxia-exposed human choroidal endothelial cells were employed. In vivo, eriocitrin or the anti-vascular endothelial growth factor agent conbercept was administered via intravitreal injection. Fundus fluorescein angiography, indocyanine green angiography, and immunofluorescent staining were used to assess choroidal neovascularization. In vitro, human choroidal endothelial cells were treated with eriocitrin under hypoxic conditions. Cell proliferation, migration, and tube formation were evaluated using 5-ethynyl-2'-deoxyuridine, Transwell, and tube formation assays. Protein levels of mitogen-activated protein kinase phosphatase-1, p-p38, and p-JNK were detected by Western blot. RESULTS:Eriocitrin significantly attenuated choroidal neovascularization development and vascular leakage in mice in a dose-dependent manner, with a 4 µg dose showing efficacy comparable to conbercept. In hypoxic human choroidal endothelial cells, eriocitrin inhibited proliferation, migration, and tube formation. Under hypoxic conditions, eriocitrin elevated mitogen-activated protein kinase phosphatase-1 protein levels and reduced p-p38 and p-JNK expression. Inhibition of mitogen-activated protein kinase phosphatase-1 diminished the suppressive effects of eriocitrin on hypoxia-induced pro-angiogenic activities. CONCLUSION:Eriocitrin alleviates choroidal neovascularization by promoting mitogen-activated protein kinase phosphatase-1 activity in choroidal endothelial cells, thereby inhibiting the p38 and JNK signaling pathways. These findings suggest eriocitrin as a potential therapeutic agent for choroidal neovascularization.

With the advancement of hydrogel technology, increasing attention has been drawn to hydrogel microspheres (HMs) due to their versatile biomedical applications. HMs play pivotal roles in biomedical applications, such as drug delivery, cell culture, regenerative medicine, wound healing, and tumor immunity. Composed of diverse biobased materials and fabricated through various preparation methods, HMs offer unique structural and functional advantages. This review focuses on the latest findings to provide a more comprehensive understanding of HMs for biomedical applications. Their therapeutic potential across multiple disease contexts is highlighted, and emerging trends and challenges are discussed. By consolidating current knowledge, this work aims to inspire further research and accelerate the clinical translation of HMs.

Background:After distal pancreatectomy (DP), clinically relevant postoperative pancreatic fistula (CR-POPF) is a critical complication that adversely affects the prognosis of patients. The present study identified the risk factors for CR-POPF occurrences, as well as developing a nomogram to predict their risk after DP. Methods:We retrospectively examined 300 medical records, obtaining the patients' preoperative clinical baseline data, laboratory indicators, preoperative computed tomography (CT) data, and intraoperative clinical information. We determined CR-POPF independent risk factors using univariate as well as multivariate logistic regression analyses. We created a risk nomogram based on these variables and used the bootstrap method for internal validation. Area under the curve (AUC) assessed the nomogram's predictive power. The nomogram's clinical value and viability were evaluated using decision curve analysis (DCA) as well as clinical impact curve (CIC). Results:CR-POPF developed in 84 of the 300 patients (28.0% incidence). CR-POPF was found to be independently risked by operation time (P=0.002), preoperative C-reactive protein (CRP) levels (P<0.001), CT (pancreas)-to-CT (psoas major) ratio (P<0.001), and pancreatic thickness (PT) at transection site (P<0.001). The nomogram's AUC was 0.901, which, along with the DCA, highlighted the nomogram's excellent performance, surpassing those of four alternative CR-POPF prediction models. The nomogram has an immense net clinical advantage, according to the CIC. Conclusions:The developed nomogram can be useful in identifying high-risk patients and formulating individualized perioperative plans to prevent the risk of CR-POPF formation in patients undergoing DP.

Diffuse large B-cell lymphoma (DLBCL) is an aggressive type of non-Hodgkin lymphoma characterized by high rates of relapse and limited responsiveness to standard chemotherapy. Selinxor, a selective inhibitor of XPO1, exhibited antitumor activity in various cancers. However, clinical trial results revealed that selinexor monotherapy exhibited unsatisfactory efficacy in DLBCL. Our study indicated that XPO1 expression was increased in DLBCL and was correlated with poor outcomes of DLBCL patients. Comprehensive proteomic and transcriptomics analysis showed that selinexor has significant impacts on various biological processes in DLBCL. Furthermore, we explored combination strategies involving selinexor to enhance DLBCL treatment. We examined the combined effects of selinexor with decitabine (DAC) and lenalidomide (LEN), and found that selinexor exhibited a synergistic effect with DAC against DLBCL. Further analysis revealed that DAC exerted a synergistic antitumor effect with selinexor by reversing the DNMT1 expression and DNA methylation alterations induced by selinexor. Overall, these findings provided valuable insights into the global impact of selinexor on DLBCL. The combination therapy of selinexor and DAC emerges as a highly promising strategy for effectively treating DLBCL, holding great potential for clinical application.

Ferritinophagy, the selective autophagic degradation of ferritin to release iron, is emerging as a critical regulator of iron homeostasis and a key player in the pathogenesis of various liver diseases. This review comprehensively examines the mechanisms, regulation, and multifaceted roles of ferritinophagy in liver health and disease. Ferritinophagy is intricately regulated by several factors, including Nuclear Receptor Coactivator 4 (NCOA4), Iron regulatory proteins and signaling pathways such as mTOR and AMPK. These regulatory mechanisms ensure proper iron utilization and prevent iron overload, which can induce oxidative stress and ferroptosis. In liver diseases, ferritinophagy exhibits dual roles. In liver fibrosis, promoting ferritinophagy in hepatic stellate cells (HSCs) can induce cell senescence and reduce fibrosis progression. However, in non-alcoholic fatty liver disease (NAFLD), chronic ferritinophagy may exacerbate liver injury through iron overload and oxidative stress. In hepatocellular carcinoma (HCC), ferritinophagy can be harnessed as a novel therapeutic strategy by inducing ferroptosis in cancer cells. Additionally, ferritinophagy is implicated in drug-induced liver injury and sepsis-associated liver damage, highlighting its broad impact on liver pathology. This review also explores the crosstalk between ferritinophagy and other selective autophagy pathways, such as mitophagy and lipophagy, which collectively influence cellular homeostasis and disease progression. Understanding these interactions is essential for developing comprehensive therapeutic strategies targeting multiple autophagy pathways. In summary, ferritinophagy is a complex and dynamic process with significant implications for liver diseases. This review provides an in-depth analysis of ferritinophagy’s regulatory mechanisms and its potential as a therapeutic target, emphasizing the need for further research to elucidate its role in liver health and disease.

Background:Placement of a support tube during pancreaticojejunal anastomosis has been shown to reduce the incidence of postoperative pancreatic fistula in patients with a non-dilated pancreatic duct. However, increasing reports of long-term complications, such as the presence of an indwelling support tube in the anastomosis, have raised concerns. Despite this, short-term complications, such as gastrointestinal perforation in the absence of tube displacement, remain relatively rare. Case Description:This report describes a 59-year-old female patient who underwent radical pancreaticoduodenectomy for pancreatic ductal adenocarcinoma (T2N0M0). On the 8th postoperative day, she developed back pain, followed by a sudden increase in body temperature, elevated inflammatory markers, and a significant increase in amylase levels in the drainage fluid. Enhanced abdominal computed tomography revealed that one side of the pancreatic duct support tube had penetrated the abdominal cavity, resulting in posterior peritoneal effusion. Emergency removal of the support tube and repair of the intestinal perforation were performed. The patient had an uneventful recovery after surgery and was discharged on day 20 following the second operation. Conclusions:In cases of postoperative abdominal or low back pain accompanied by signs of infection, complications related to the support tube should be considered. Further studies are needed to evaluate the necessity of placing a pancreatic duct support tube, as well as the timing for its removal after the completion of the anastomosis.

Background The anatomical relationship between the pancreatic tail and splenic vessels influences both pancreatic resection completeness and spleen preservation success in robotic spleen-preserving left-sided pancreatectomy (RSPLP). However, surgical strategies for managing the pancreatic tail during RSPLP remain unreported Methods Clinical data from 46 consecutive patients undergoing robot-assisted left-sided pancreatectomy with intended spleen preservation were analyzed. Pancreatic tails were classified into four anatomical types (I-IV) based on their relationship with splenic vessels or tumors. Results The RLP group (spleen preservation failure) exhibited a higher proportion of type IV anatomy than the RSPLP group (spleen preservation success) (76.92% vs. 18.18%, P<0.05). Type III/IV anatomy was associated with significantly lower spleen preservation rates (52.17% vs. 91.30%, P=0.003), larger tumor size [4.0 (3.5-5.8) vs. 2.8 (2.2-4.6) cm, P=0.026], greater intraoperative blood loss [134 (58-295) vs. 85 (45-180) mL, P=0.017], and longer operative time (257.3±62.29 vs. 220.78±53.05 min, P=0.038) compared to type I/II. Conclusion We proposed the preoperative classification of pancreatic tail type and the “pendulum separation” technique, and found that it was challenging to preserve the spleen in type III and IV pancreatic tails due to their complex anatomical relationship with the splenic vessel and tumor.

e16440 Background: This study aimed to investigate overall survival (OS) in patients with surgically resected pancreatic cancer and examined the distribution of intratumoral microbiomes across distinct sites within pancreatic tumors. Methods: Patients were selected from the Surveillance, Epidemiology, and End Results (SEER) registry (2000–2021 dataset). Kaplan-Meier survival analysis and multivariable Cox proportional hazards models were used to assess overall and site-specific survival outcomes. A retrospective analysis was initiated to verify prognostic factors related with survival in surgically resected pancreatic cancer patients. To compare differential profiles of intratumoral microbiota, 16S rRNA gene sequencing was conducted on tissue samples from 7 patients with pancreatic head cancer (PHC) and 7 with pancreatic body/tail cancer (PBTC). Correlations between microbiota and prognosis, as well as associated gene expression changes, were analyzed using data from The Cancer Genome Atlas (TCGA). The effect of microbiota on tumor metastasis was confirmed by a pulmonary metastasis model of mouse pancreatic cancer. Results: A total of 78,548 patients were identified. The OS of surgically resected patients with PBTC was significantly better than that of patients with PHC. Multivariate analysis showed that male, older age, pancreatic head location, M1 and stage IIB/III/IV were independent predictors of poorer survival. Retrospective analysis involved 162 patients of our center verified that OS of PBTC was significantly longer than PHC. Microbiota was detected in tumor tissue of pancreatic cancer patients. The microbial species diversity in PHC tissues was significantly higher than in PBTC tissues, with microbial compositions across six phylogenetic levels highlighting specific differences between PHC and PBTC patients. At the genus level, 17 bacterial genera were identified, among which Sphaerotilus and Dyella , significantly enriched in PBTC patients, were associated with improved survival, as indicated by TCGA survival data. Absence of these bacteria might contribute to immune deficiency, widespread activation of cancer-related pathways, and metabolic reprogramming. In vivo experiment proved that Sphaerotilus and Dyella could inhibit pulmonary metastasis of pancreatic cancer cells. Conclusions: Our study demonstrated that patients with pancreatic body/tail tumors had a notably longer median survival than those with tumors in the head region following surgical resection. Enrichment of Sphaerotilus and Dyella in PBTC was significantly associated with improved survival in pancreatic cancer patients. These findings suggest that intratumoral microbiota may play a role by modulating signaling-related genes and regulating immune status, highlighting its potential as a therapeutic target and prognostic marker of pancreatic cancer patients.

Emerging evidence underscores the pivotal yet context-dependent role of butyrate metabolism in oncogenic progression; however, the mechanistic landscape of its associated genetic determinants in prostate carcinogenesis remains poorly characterized. This gap presents a critical opportunity to delineate novel metabolic checkpoints that may offer therapeutic vulnerabilities for advanced prostate malignancies. Analyzing prostate cancer (PC) patients from TCGA and GEO databases, we identified 320 BMGs and stratified tumors into two butyrate metabolism-associated clusters. Machine learning and single-cell transcriptome analysis are used for further study. Luciferase reporter assay, qRT-PCR, FISH, and functional assays are applied to investigate the role of FOS. BMC1 correlated with aggressive phenotypes and stromal-rich tumor microenvironments, while BMC2 was linked to cell cycle regulation and DNA repair. A machine learning-derived RSF + GBM prognostic model demonstrated robust predictive accuracy for biochemical recurrence (training C-index: 0.85). BM scores are further associated with tumor mutation burden and differential drug sensitivities. FOS is overexpressed in PC, promotes proliferation and migration via transcriptional suppression of tumor-suppressive miR-27b. Clinical cohorts confirmed FOS’s correlation with advanced T stages and recurrence risk. These findings establish BM-based stratification as a prognostic tool and implicate the FOS-miR-27b axis as a therapeutic target, bridging metabolic heterogeneity with molecular mechanisms in PC progression. Our research offers a critical opportunity to delineate novel metabolic checkpoints that may offer therapeutic vulnerabilities for advanced prostate malignancies.

To examine the learning curve and perioperative outcomes of robotic-assisted distal pancreatectomy (RDP) conducted at a single center and to contextualize these findings against existing literature. A single expert surgical team conducted RDP on 106 patients at the Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Soochow University, from January 2021 to December 2024. To explore the learning pattern of operative duration, cumulative sum (CUSUM) analysis was utilized. Breakpoints in textbook outcomes (TBO) for RDP were subsequently identified through the application of a two-segment regression model. Perioperative indicators before and after the breakpoints were compared. The cohort showed a median operative time of 230 min, with 74.53

Liver metastasis is the most frequent site of distant metastasis in pancreatic ductal adenocarcinoma (PDAC), significantly contributing to poor prognosis. This study aims to develop and validate a machine learning (ML) model for predicting early liver metastasis (ELM) following pancreatic cancer surgery. This retrospective study included 407 pancreatic cancer patients who underwent surgery at the First Affiliated Hospital of Soochow University between January 2015 and December 2023, aiming to develop and validate a predictive model. Seven ML algorithms were employed to predict the risk of liver metastasis within one year after surgery. The training cohort (n = 284) was used for model development and hyperparameter tuning, while the internal validation cohort (n = 123) was employed to assess predictive performance. Shapley additive explanations (SHAP) were applied to elucidate the decision-making process of the best-performing model. To assess the generalizability of the model, 131 PDAC patients from the Affiliated Hospital of Nantong University were included as an external validation cohort. A total of 194 patients (36.1

PURPOSE:This study compared the incidence of postoperative pancreatic fistula (POPF) between standard invagination pancreaticojejunostomy (PJ) and an improved PJ technique after pancreaticoduodenectomy and evaluated the clinical utility of the improved PJ procedure. METHODS:Clinical and postoperative data of 363 patients who underwent pancreaticoduodenectomy at the First Affiliated Hospital of Soochow University from February 2018 to October 2021 were analyzed retrospectively. In our cohort, 155 patients underwent the improved PJ technique (group A), and 208 underwent standard invagination PJ (group B). Data on demographic characteristics, pathological nature, intraoperative factors, and postoperative complications, including POPF, were collected and analyzed. RESULTS:There were no significant between-group differences in demographic characteristics (p > 0.05). The improved PJ technique was associated with a significantly lower incidence of clinically relevant POPF (CR-POPF) in the total cohort (11.6 % vs. 26.4 %, p < 0.001) and in the subgroup with high fistula risk scores (16.0 % vs. 38.6 %, p < 0.001). CONCLUSION:The improved invagination PJ technique reduces the incidence of CR-POPF and improves prognosis.

Background The aim of this study was to develop a nomogram to predict the risk of developing clinically relevant postoperative pancreatic fistula (CR-POPF) after pancreaticoduodenectomy (PD) using preoperative clinical and imaging data. Methods The data of 205 patients were retrospectively analyzed, randomly divided into training ( n = 125) and testing groups ( n = 80). The patients’ preoperative laboratory indicators, preoperative clinical baseline data, and preoperative imaging data [enhanced computed tomography (CT), enhanced magnetic resonance imaging (MRI)] were collected. Univariate analyses combined with multivariate logistic regression were used to identify the independent risk factors for CR-POPF. These factors were used to train and validate the model and to develop the risk nomogram. The area under the curve (AUC) was used to measure the predictive ability of the models. The integrated discrimination improvement index (IDI) and decision curve analysis (DCA) were used to assess the clinical feasibility of the nomogram in relation to five other models established in literature. Results CT visceral fat area ( P = 0.014), the pancreatic spleen signal ratio on T1 fat-suppressed MRI sequences ( P < 0.001), and CT main pancreatic duct diameter ( P = 0.001) were identified as independent prognostic factors and used to develop the model. The final nomogram achieved an AUC of 0.903. The IDI and DCA showed that the nomogram outperformed the other five CR-POPF models in the training and testing cohorts. Conclusion The nomogram achieved a superior predictive ability for CR-POPF following PD than other models described in literature. Clinicians can use this simple model to optimize perioperative planning according to the patient’s risk of developing CR-POPF.

In robotic spleen-preserving distal pancreatectomy (RSPDP), the relationship between the pancreatic tail and splenic vessels affects whether the distal pancreas can be resected completely or the spleen can be preserved successfully. However, no study has reported surgical strategies for the management of pancreatic tails during RSPDP. In this study, the clinical data of 46 patients who consecutively received robot-assisted distal pancreatectomy (DP) with intended splenic preservation were analyzed. The pancreatic tails were classified into four anatomical variations based on the relationship between splenic vessels and pancreatic parenchyma or tumor. The RDP group had more type IV cases (76.92% vs. 18.18%, P < 0.05) and less type II cases (7.69% vs. 39.39%, P < 0.05) compared with the RSPDP group. Compared with the type I/II group, the type III/IV group had a significantly lower spleen-preserving rate (52.17% vs. 91.30%, P = 0.003). Moreover, the spleen preservation rate was significantly elevated in the second period (2023.01-2024.01, n = 29) than in the first period (2021.01-2022.12, n = 17) (82.76% vs. 52.94%, P = 0.030). We proposed the preoperative classification of pancreatic tail type and the “pendulum separation” technique, and found that it was challenging to preserve the spleen in type III and IV pancreatic tails due to their complex anatomical relationship with the splenic vessel and tumor.

Background Pancreatic carcinoma (PC) is a highly lethal cancer with an increasing mortality rate, its five-year survival rate is only approximately 4%. N6-methyladenosine (m6A) modification is the most common posttranscriptional modification of RNA, it could affect tumor formation by regulating m6A modifications in the mRNA of key oncogenes or tumor suppressor genes. However, its role in PC remains unclear. Methods We combined bioinformatic analysis with in vitro and in vivo experiments to investigate the expression profile of methylation modulators and identify key m6A regulators in the progression of PC. Further study focused on exploring the target genes binding to the regulators through RIP and immunofluorescence staining experiment. Results TCGA and Gene Expression Omnibus (GEO) analyses revealed an overall increasing trend in the expression of m6A regulators in PC, and consensus clustering analysis of m6A modification showed that the expression of regulators was negatively correlated with the survival rate. LASSO-Cox regression analysis revealed that IGF2BP2, METTL3, ALKBH5 and KIAA1429 were associated with hazard ratios (HR), but only IGF2BP2 was sufficiently appropriate for the m6A survival prognosis model. The IHC and WB results verified high protein expression of IGF2BP2 in PC, and IGF2BP2 knockdown inhibited the proliferation and migration of PC cells. We predicted and verified B3GNT6 was observably regulated by IGF2BP2 via RIP assays. In addition, IF staining confirmed the co-expression of IGF2BP2 and B3GNT6. The tumor-promoting effect of IGF2BP2 and its co-expression with B3GNT6 were verified in an animal model. Conclusions Elevated m6A levels promote PC progression. IGF2BP2 is a credible marker and modulates B3GNT6 mRNA stability, indicating that IGF2BP2 is a potential prognostic marker and therapeutic target in PC progression.

Background: Hypoxia plays an important role in the development of pancreatic cancer (PCA). However, there is few research on the application of hypoxia molecules in predicting the prognosis of PCA. We aimed to establish a prognostic model based on hypoxia-related genes (HRGs) for PCA to discover new biomarkers, and to reveal the potential of this prognostic model for evaluating the tumor microenvironment (TME). Methods: Univariate Cox regression analysis was used to identify HRGs associated with overall survival (OS) of PCA samples. A hypoxia-related prognostic model was established based on least absolute shrinkage and selection operator (LASSO) regression analysis in The Cancer Genome Atlas (TCGA) cohort. The model was validated in the Gene Expression Omnibus (GEO) datasets. The Cell-type Identification by Estimating Relative Subsets of RNA Transcripts (CIBERSORT) algorithm was used to estimate the infiltration of immune cells. A wound healing assay and transwell invasion assay were used to explore the biological functions of target genes in PCA. Results: A total of 18 HRGs were differentially expressed between the tumor and normal pancreatic tissue, 4 (BHLHE40, ENO1, SDC4, and TGM2) of which were selected to construct a prognostic model. According to this model, patients in the high-risk group had a less favorable prognosis. Furthermore, the proportion of M0 macrophages was significantly higher in high-risk tissue-type patients, whereas naive B cells, plasma cells, CD8(+) T cells, and activated CD4(+) memory T cells were significantly lower. The expression of BHLHE40 in PCA cells was significantly up-regulated under hypoxic conditions. Moreover, BHLHE40 was shown to regulate the transcription and expression of the downstream target gene TLR3. The wound healing assay and transwell invasion assay indicated that BHLHE40 mediated PCA cell migration and invasion by targeting the downstream gene TLR3. Conclusions: The hypoxia-related prognostic model established by the expression pattern of 4 HRGs can be used to predict the prognosis and assess the TME of PCA patients. Mechanically, activation of the BHLHE40/TLR3 axis is responsible for the promoted invasion and migration of PCA cells in a hypoxic environment.

目的 总结本中心应用达芬奇机器人Xi系统在肝切除中的效果和布孔和程序化操作方面的经验.方法 回顾性分析苏州大学附属第一医院肝胆胰外科2020年10月至2022年5月期间施行达芬奇机器人肝切除手术的38例患者的临床和病理资料.结果 38例均采用达芬奇Xi系统成功完成肝切除手术,1例因手术创面止血不满意中转开腹.术中采用反L型布孔加快装机步骤,减少频繁更换器械的困扰,适用于大部分肝切除术.右半肝切除中超声刀优先置于4号臂进行肝门处理,随后切换至2号臂左手操作断肝,可以保证良好的切肝角度,联合两侧机械臂的配合,形成类似于"三叉戟"的切除模式.布置1～2个助手孔,利用吸引器及电凝设备保持术野清晰.术后病理:肝脏恶性肿瘤29例(均为R0切除),良性肿瘤5例,肝内外胆管结石3例,肝脓肿1例.中位手术时间250(126)min,术中出血量100(110)mL,手术并发症发生率为7.9％(3/38),均经保守治疗治愈,无围手术期死亡.34例患者术后留置引流管,置管时间4(1)d,术后住院时间6(2)d.结论 机器人辅助肝切除术安全可行,机器人平台的信息整合以及灵活操作的独有特点,有助于精准解剖性肝切除开展和进一步减少肝切除手术的创伤.程序化手术布局及手术流程,有利于缩短机器人肝切除术的学习曲线.

This study aimed to investigate the role of the long non-coding RNA (lncRNA) LINC00342-207 (LINC00342) in the development and progression of primary hepatocellular carcinoma (HCC). Forty-two surgically resected HCC tissues and corresponding paracancerous tissues were collected from October 2019 to December 2020 and examined for lncRNA LINC00342, microRNA (miR)-19a-3p, miR-545-5p, miR-203a-3p, cell cycle protein D1 (CyclinD1/CCND1), murine double minute 2 (MDM2), and fibroblast growth factor 2 (FGF2) expression. The disease-free survival and overall survival of patients with HCC were followed up. HCC cell lines and the normal hepatocyte cell line HL-7702 were cultured and the expression level of LINC00342 was measured. HepG2 cells were transfected with LINC00342 siRNA, LINC00342 overexpression plasmid, miR-19a-3p mimics and their corresponding suppressors, miR-545-5p mimics and their corresponding suppressors, and miR-203a-3p mimics and their corresponding suppressors. The proliferation, apoptosis, migration, and invasion of HepG2 cells were detected. Stably transfected HepG2 cells were inoculated into the left axilla of male BALB/c nude mice, and the volume and quality of transplanted tumors as well as the expression levels of LINC00342, miR-19a-3p, miR-545-5p, miR-203a-3p, CCND1, MDM2, and FGF2 were examined. LINC00342 played an oncogenic role in HCC and exhibited inhibitory effects on proliferation, migration, and invasion, and promoted the apoptosis of HepG2 cells. Moreover, it inhibited the growth of transplanted tumors in vivo in mice. Mechanistically, the oncogenic effect of LINC00342 was associated with the targeted regulation of the miR-19a-3p/CCND1, miR-545-5p/MDM2, and miR-203a-3p/FGF2 axes.

Objective:To investigate the clinical manifestations ,diagnosis and treatment of autoimmune pancreatitis (AIP).Methods:The clinical data of 17 AIP patients admitted at the First Affiliated Hospital of Soochow University from Apr 2021 to Jan 2023 were retrospectively analyzed.Results:Among the 17 AIP patients, 12 were male (70.6%). Ten complained abdominal pain (58.8%), and 8 had jaundice (47.1%). Fifteen patients had elevated IgG4 levels more than twice the normal value (88.2%), and 14 patients had elevated liver enzymes (82.3%). The imaging manifestations of 17 patients were all diffuse or limited enlargement of the pancreas. All patients had diffuse or limited enlargement of the pancreas, some were accompanied by a dilatation of the pancreatic duct or bile duct. Fourteen patients were diagnosed by combining the clinical manifestations, imaging examination, laboratory examination, and puncture pathology, and in 3 cases the diagnosis was confirmed by postoperative pathology. Fourteen patients showed significant improvement in clinical symptoms and imaging manifestations after hormone therapy, 2 patients stopped hormone medication on their own after the improvement of the imaging, and 1 suffered recurrence,which was responsible to hormone readministration.Conclusions:AIP, as a rare and easily misdiagnosed immune disease, involves the pancreas leading to an inflammatory response and often encroaches peri-pancreatic areas such as the bile ducts, causing biliary stenosis and jaundice. Most patients respond well to glucocorticoid therapy and surgery was usually not indicated in those with definite AIP diagnosis.