Extracellular accumulation of β amyloid (Aβ) peptides in the brain is thought to be a pathological hallmark and initial event before the symptom starts of Alzheimer's patients. Herein, we developed two series of benzo[d]thiazole-based small-molecule compounds (BM1-BM4, BPM1-BPM4) with a donor-acceptor (D-A) or donor-π-acceptor (D-π-A) architecture, respectively, based on structure-activity relationship. Among them, the optimized BPM1 not only displayed the highest binding affinity to Aβ aggregates over other proteins or Aβ monomers, but was readily activated its fluorescence with 10-fold fluorescence enhancement, allowing for specifically and sensitively detecting Aβ aggregates. BPM1 also exhibits several other advantages including low molecular weight, low cytotoxicity and excellent biological stability. Besides, cell staining results confirmed that SK-N-BE(2) cells can be fluorescently lighted up as well as cell permeability and damage when treated with BPM1-bound Aβ1-42 aggregates.

OBJECTIVE:To investigate the diagnostic efficacy of seven glomerular filtration rate (GFR) evaluation formulas Schwartz2009, Schwartz1976, Counahan-Barratt, Filler, CKD-EPIscysc, Cockrofi-Gault, CKD-EPIScysC-Scr in high concentration of methotrexate (HDMTX) chemotherapy dose adjusted cut-off point (GFR ≤85 ml/min) in children with acute lymphoblastic leukemia (ALL).METHODS:One hundred and twenty-four children with ALL were included in the study. GFR determined by renal dynamic imaging (sGFR) was used as the standard to evaluate the accuracy, consistency of eGFR calculated by seven formulas and sGFR, and the diagnostic efficacy of each formula when the sGFR ≤85 ml/min boundary.RESULTS:All of the accuracy of eGFR estimated by Schwartz2009 were greater than 70% in the 0-3, >4 and ≤6, >6 and ≤9, >9 and ≤16 years old group and male group, and the consistency exceeded the professional threshold. When the sensitivity of the ROC curve sGFR ≤85 ml/min was 100% of CKD-EPIscysc in the 0-3, >3 and ≤4 years old group, Filler in the >3 and ≤4 years old group, and Cockrofi-Gault in the >6 and ≤9 years old group, the specificity was 73.02%, 78.95%, 78.95%, 69.32%, respectively, and the AUC under the ROC curve was the largest (P<0.05).CONCLUSION:Schwartz2009 formula predicts the highest accuracy of eGFR in the 7 glomerular filtration rate. CKD-EPIscysc, Filler, and Cockrofi-Gault formulas have more guiding signi-ficance for the adjustment of HDMTX chemotherapy in pre-adolescence in children with ALL when sGFR ≤85 ml/min.

目的 探讨肺内单发恶性肿瘤18F-FDG PET/CT显像时最大标准化摄取值(SUVmax)与肿瘤细胞生长指数(Ki-67)的相关性,为患者预后评估提供参考.方法 回顾性分析2016年4月至2018年7月安徽医科大学第二附属医院36例肺内单发恶性肿瘤患者资料,术前均行18 F-FDG PET/CT检查,测量病灶最大层面长径,病灶最大层面长径以3 cm为界,分为≤3 cm肺结节组和>3 cm肺肿块组.测定并计算肿瘤SUVmax早期值、SUVmax延迟期值、ΔSUVmax及滞留指数(RI),并采用Spearman秩相关分析与术后病理类型、Ki-67的相关性;采用非参数检验不同大小病灶的ΔSUVmax值及RI值差异、不同病理类型问ΔSUVmax值及RI值差异.结果 36例肺癌病灶SUVmax早期值为1.21～30.11,中位数为9.350(4.855,14.660).SUVmax延迟期值为1.53～31.71,中位数为10.450(6.938,17.233).原发灶最大层面长径为1.11～8.13 cm,中位数为2.700(2.075,3.668)cm.Ki-67为1％～90％.Spearman秩相关分析结果显示,SUVmax早期值与Ki-67的表达均呈低度相关(r=0.459,P<0.05);SUVmax延迟值、ΔSUVmax与Ki-67的表达均呈显著相关(r=0.584、0.506,P<0.05),RI与Ki-67问呈微弱相关(r=0.183,P=0.286).不同病理类型及不同大小病灶问ΔSUVmax值和RI值比较,差异无统计学意义(P>0.05).结论 肺内单发病灶行18F-FDG PET/CT显像时,不同肿瘤大小、病理类型对ΔSUVmax值和RI值影响不大.SUVmax越高,表明肿瘤增殖活性越大,可预估患者预后.

目的 核素肾动态显像评估12种肾小球滤过率(GFR)计算公式在估算肾脏疾病患者GFR中适用性.方法 选择肾脏疾病患者137例,其中男性84例,女性53例;年龄16～85岁,中位年龄52.5岁;慢性肾脏病(CKD)84例,非CKD53例.行99m锝-二乙三胺五乙酸(99mTc-DTPA)肾动态单光子发射体层摄影术(SPECT)测定GFR.以SPECT所测得的GFR(iGFR)作为参考标准,以临床应用的12种方程(如MDRD公式、CKD-EPI、CG公式、Hojs公式、Filler公式)估算GFR(eGFR)为对照,分析各eGFR与iGFR的相关性、偏差、精确度、准确率、一致性及各公式预测早期CKD[或肾功能下降iGFR＜60mL/(min·1.73 m2)]诊断效能.结果 各eGFR与iGFR均显示相关性(P＜0.05).在数值估算上,CKD组中CKD-EPI糖尿病估算的eGFR与iGFR有较小偏差、较高精确度、较高符合率及一致性,非CKD组一致性超过预定的界限.12种公式预测早期CKD[或肾功能下降iGFR＜60 mL/(min·1.73m2)]诊断效能受试者工作特性(ROC)曲线下面积示CKD-EPI糖尿病测量的eGFR曲线下面积、Youden指数最大,非CKD组Hojs公式、Filler公式测量的eGFR曲线下面积、Youden指数最大.结论 12种公式预测的eGFR均存在不同程度偏差,CKD-EPI糖尿病在CKD、非 CKD两组中预测GFR值最适用;CKD-EPI糖尿病预测CKD组早期CKD发生诊断效能最大,Hojs公式、Filler公式预测非CKD组早期肾功能下降[GFR＜60 mL/(min·1.73 m2)]发生诊断效能最大.

The title compound, C 19 H 13 N, which crystallizes in the monoclinic C 2/ c space group with one half-molecule in the asymmetric unit, was synthesized by Suzuki–Miyaura cross-coupling reaction of 9-bromoanthracene with pyridin-4-ylboronic acid and purified by column chromatography on silica gel. Light-yellow crystals of 4-(anthracen-9-yl)-pyridine suitable for X-ray diffraction were collected by the solvent evaporation method. In the crystal, pairs of molecules are connected by intermolecular C—H...π (pyridine) interactions [ d (H7... Cg ) = 2.7391 (2) Å], forming cyclic centrosymmetric dimers, further resulting in an infinite one-dimensional linear chain along the c -axis direction. Weak face-to-face π–π stacking interactions [ d ( Cg ... Cg ) = 3.6061 (2) Å] link neighboring lamellar networks into the supramolecular structure.

Recently, immune response modulation at the epigenetic level is illustrated in studies, but the possible function of RNA 5-methylcytosine (m5C) modification in cell infiltration within the tumor microenvironment (TME) is still unclear. Three different m5C modification patterns were identified, and high differentiation degree was observed in the cell infiltration features within TME under the above three identified patterns. A low m5C-score, which was reflected in the activated immunity, predicted the relatively favorable prognostic outcome. A small amount of effective immune infiltration was seen in the high m5C-score subtype, indicating the dismal patient survival. Our study constructed a diagnostic model using the 10 signature genes highly related to the m5C-score, discovered that the model exhibited high diagnostic accuracy for PTC, and screened out five potential drugs for PTC based on this m5C-score model. m5C modification exerts an important part in forming the TME complexity and diversity. It is valuable to evaluate the m5C modification patterns in single tumors, so as to enhance our understanding towards the infiltration characterization in TME.

目的 探讨核素甲状腺显像在131 I治疗甲状腺两叶不均等Graves病病人中的应用,为临床Graves病的131 I个性化治疗提供依据.方法 选择2010年1月至2015年12月间,在安徽医科大学第二附属医院核医学科首次接受131 I治疗的Graves病病人194例.131 I治疗前均行甲状腺核素显像,勾画甲状腺两叶ROI(region of interest),分别记录两叶放射性计数,根据两叶放射性计数比值差值绝对值分为两组:A组为比值差值≥20％,共40例;B组为差值<5％,共154例.参照131 I治疗格雷夫斯甲亢指南(2013版)又分为:治愈组(完全缓解和甲减)和未愈组(部分缓解、无效和复发).结果 (1)A、B组病人治愈率分别为75.00％、89.61％,两组治愈率间比较差异有统计学意义(χ2=5.84,P<0.05);(2)40例Graves病病人中甲状腺两叶长轴比值、短轴比值及面积比值的差值与放射性计数比值的差值均无相关性(均P>0.05);(3)A、B两组病人的治疗剂量分别为(474.00±310.80)MBq、(393.68±306.73)MBq,差异无统计学意义(均P>0.05);而A组中未愈组和治愈组与B组未愈组和治愈组比较均差异有统计学意义(均P<0.05).结论 (1)131I治疗甲状腺两叶大小不均等Graves病的效果明确,但较均等增大病人的重复治疗机率增大,可适当增加131 I治疗计划量;(2)甲状腺的长轴、短轴及面积等指标不能替代放射性计数来评估Graves病病人甲状腺两叶的不均等性.

目的 探讨全身骨扫描及MRI检查在诊断椎体终板炎中的应用价值.方法 收集安徽医科大学第二附属医院2014年6月至2016年12月行全身骨扫描,同时在骨扫描前后1个月内行脊柱或局部MRI检查并诊断为终板炎的38例患者的临床资料,由2位有经验的核医学科医师及放射科医师独立进行骨扫描及MRI图像的分析.结果 38例患者中,MRI共发现85个病灶,各部位占比为颈椎3.53％(3/85)、胸椎7.06％(6/85)、腰椎44.71％(38/85)、颈胸椎5.89％(5/85)、胸腰椎7.06％(6/85)、腰骶椎31.76％(27/85).Modic分型诊断为Ⅰ型者18例,Ⅱ型者18例,另2例介于Ⅰ型及Ⅱ型间,Ⅲ型0例.MRI诊断为椎体终板炎且骨扫描为阳性的病例中,共发现病灶数30个,各部位占比为腰椎60.0％(18/30)、腰骶椎20％(6/30)、胸椎10％(3/30)、胸腰椎6.67％(2/30)、骶椎3.33％(1/30).将全身骨扫描与MRI检查结果按符合程度进行讨论,包括阴性符合19例、完全符合6例、部分符合7例、不符合2例、部分不符合4例.绪论MRI在诊断椎体终板炎方面有明显的优势,骨扫描进行骨转移瘤诊断时,应充分结合MRI图像,可提高对椎体终板炎的诊断准确率.

Objective To investigate the application value of chronic kidney disease epidemiology collaboration (CKD-EPI) equations and amendatory equations for calculating glomerular filtration rate (GFR) in Chinese patients with diabetes,including CKD-EPI serum creatinine (Scr) equation (CKD-EPLcr),CKD-EPI serum cystatin C (ScysC) equation (CKD-EPLcysc),combination of CKD-EPLcr and CKD-EPIScrSCysC (CKD-EPISr SCysC),Asian modified CKD-EPI equation (CKD-EPIAsian),Chinese diabetes CKD-EPI equation (CKD-EPIDiabctes),refitted modification of diet in renal disease (MDRD) equation (rMDRD),Cockcroft-Gault equation (CG) and Simplified MDRD equation.Methods Serum creatinine (Scr) was detected using basic picric acid method,serum cystatin C (CysC) was measured by Latex enhanced turbidimetric,and the reference value of GFR was worked out by 99Tcm-DTPA renal dynamic imaging and standardization of body surface area.Relation of Scr,Scysc,GFR measured by every equations and rGFR were analyzed.The accuracy of 30％ of each equation,the consistency and deviation of the evaluation of renal function by each equation were compared.The diagnostic efficacy in GFR＜60 ml/(min · 1.73 m2) was compared using the receiver operating characteristic (ROC) curve.Results The rGFR had negative correlation with Scr、ScysC (both P＜0.001).The rGFR had positive correlation with GFR measured by CKD-EPIScr,CKD-EPIScysC,CKD-EPIS-SCysC,CKD-EPIAsian,CKD-EPIDiabetes rMDRD,CG and Simplified MDRD (all P＜0.001).The coincidence rate between 30％ of rGFR and GFR measured byCKD-EPIDiabetes and simplified MDRD were higher.There was no significant difference of rGFR and GFR measured by CKD-EPIscr,CKD-EPIScysC,CKD-EPIScr-SCysC,CKD-EPIAsian,CKD-EPIDiabetes,rMDRD,CG and Simplified MDRD.GFR measured by CKD-EPIDiabetes has the best consistent degree with rGFR.ROC curve showed that the area under curve of GFR measured by CKD-EPIDiabctes was largest and the Youden index was the highest.Conclusion There are different degrees of deviation in eGFR predicted by the eight kinds of equations.The accuracy and consistency of CKD-EPIDiabetes in Chinese 2-type diabetes is the best.

In order to have a good knowledge of the radiation feature and source strength in the whole process of diagnosis with 99mTc,we have analyzed the variation which is caused by the primary γ ray of 99Mo and 99mTc using HPGe γspectrometer and the technology of efficient calibration without source.And we also have measured the radiation intensity of source combing active measurement with passive measurement.The experimental results show that the ratio of the main y ray emissivity changes greatly before and after the elution.The absorbed dose rate of 99 Mo-99mTc generator,syringe,and the surface of patient decrease rapidly with the increase of distance.The hands of operates can totally suffer 0.41mSv dose in the whole process of diagnosis with 99mTc in one day.

Objective To evaluate the clinical value of 99m Tc -MIBI SPECT/CT imaging for diagnosis of secondary hyperparathyroidism(SHPT),and to investigate the effect of possible factors on the parathyroid imaging.Methods A total of 35 patients were selected inthis study.Five minutes,thirty minutes (early phase)and two hours (delayed phase)after intravenous injection of 99m Tc-MIBI(740MBq),dual -phase planar imaging was performed,followed by SPECT/CT imaging over neck and chest.Thirty -five SHPT patientswere compared by both planar imaging and SPECT/CT imaging,while diagnosis of twenty -nine patients included was confirmed by pa-thology.χ2 -test was used to compare the results of planar and SPECT/CT imaging using SPSS 16.0 software.Results Compared to71.43%(25 /35)of the positive rate in planar imaging,the positive rate of the SPECT/CT imaging was 91.43%(32 /35).Difference inthe positive rates between planar and SPECT/CT imaging was statistically significant(χ2 =5.1429,P <0.05).Conclusions SPECT/CT imaging is more efficient than planar imaging for the fusion imaging and CT diagnosis of parathyroid glands with secondary hyperpar-athyroidism.SPECT/CT parathyroid imaging combined with CT scan can provide more information about localization of the lesions,which highly improve the positive rate of parathyroids.