OBJECTIVE:To explore the protective effect of Schisandrins extract on liver ultrastructure in rats with CCl4 poisoning.METHODS:Twenty five Wistar rats were randomly divided into the control group,the CCl4 poisoned 24 h and 10 d groups,the Schisandrins 24 h and 10 d groups,five groups with 5 rats in each group.The rats of the CCl4 poisoned and the Schisandrins groups were given one peritoneal injection of CCl4(200 ml/L in vegetable oil) at a dosage of 0.2 ml/100 g body weight,the rats of the control group were given the same volume of vegetable oil.Then Schisandrins extract(0.5 ml/100 g body weight)were given orally to rats of the Schisandrins group daily,while saline was given to rats of the poisoned and the control groups.The rats were sacrificed at different time points.The morphologic changes of the hepatocytes were examined by transmission electron microscopy.RESULTS:The ultrastructure of hepatocytes of rats were changed in the CCl4 poisoned 24 h group,the cytoplasm of hepatocytes was loosed and vacuolated,mitochondria were swollen,with cristae breakdown or loss,and the endoplasmic reticulum appeared lamellar.The nuclear membrane was shrunken and ruptured,and the nuclear chromatin condensed and marginated.The hepatocytic nuclei showed obvious atypia in rats of the CCl4 10 d group.By comparing with that of the CCl4 group,the ultrastructure of hepatocytes in rats of the schisandrins group was less severely damaged.CONCLUSION:The use of Schisandrins extract in the treatment of CCl4 poisoned rats exerted a protective effect on liver ultrastructure.

【Objective】To investigate the dynamic expression of integrin α1 in CCl4-intoxicated rat liver,and to explore the effect and significance of schisandrin on expression of integrin α1 in CCl4-intoxicated rat hepatocytes.【Methods】The Wistar rats were randomly divided into the control group,the intoxicated group and the treated group.The rats of intoxicated and treated groups were given a single dosage(0.2mL/100g body mass) of peritoneal injection of CCl4(200 mL/L in vegetable oil) to establish rat models of acute hepatic injury.The rats of treated group were given the extract of schisandrin(0.5 mL/100g) by intragastric administration once daily.The rats of intoxicated group were treated with normal saline(0.5 mL/100g) instead.The rats were sacrificed at different time pionts.The expression of integrin α1 in the hepatic tissue of all three groups was detected by RT-PCR and immunohistochemical method.【Results】 It showed that the expression of integrin α1 mRNA and protein in rat hepatocytes of the treated group was significantly increased compared with that of the intoxicated group.The difference between the two groups was statistically significant(P0.05).【Conclusion】Schisandrin for the treatment of acute hepatic injury induced by CCl4 can promote the expression of integrin α1 mRNA and protein in hepatocytes,which suggests that the protective effect of schisandrin on rat liver is associated with regulation of metabolism of integrin α1 in hepatic tissue.

整合素是一组广泛分布于细胞表面的跨膜糖蛋白受体,主要介导细胞与细胞、细胞与细胞外基质间的黏附,对肿瘤的侵袭、转移以及血管生成有重要的影响。研究发现,某些整合素表达的增加或减少都有可能影响肝癌的发生、发展。文章就整合素的结构、功能及其对肝癌的侵袭、转移相关关系的研究进行综述。