目的 分析高强度聚焦超声治疗子宫肌瘤过程中实施皮肤护理的效果.方法 选取2017年3月至2018年3月我院收治的子宫肌瘤患者86例,按照随机分配原则分成对照组和研究组,所有患者均实施高强度聚焦超声治疗,在此基础上对照组采用常规护理,研究组采用常规护理加皮肤护理,对两组患者效果展开深入的分析.结果 研究组治疗有效率为95.35％,对照组治疗有效率为81.40％,研究组显著高于对照组(P<0.05);研究组护理满意度为93.02％,对照组护理满意度为79.07％,对照组显著低于对照组(P<0.05).结论 对于子宫肌瘤患者而言,在进行高强度聚焦超声治疗过程中加强皮肤护理,取得比较理想的治疗效果,且患者护理满意度非常高,值得在临床上进一步的推广和使用.

Objective To investigate the activity of motif3 of mycobac-teriophage TM4 in resuscitating dormant mycobacterium tuberculosis on the basis of constructing bacteriophage TM 4 motif3 recombinant protein and the dormant model of mycobacterium tuberculosis respectively.Methods The objective motif3 fragment from bacteriophage TM 4 was amplified by polymerase chain reaction , and then cloned into the pET-28a(+) vector after purification and recovery.The recombinant plasmid was sequenced and expressed in E.coli BL21(DE3), then puri-fied and identified by Western blot.Different concentrations of motif 3 were added into dormant cells of mycobacterium tuberculosis respective-ly, then its resuscitation activity and the sterilization effect of isoniazid on resuscitated mycobacterium tuberculosis cells were detected.Results The recombinant motif3 which fused histidine residue was ob-tained.The objective protein was located at 12.65 ku.Additionally , the resuscitation activity was remarkable when the concentration of motif 3 was 1.1 ×10 4 pmol · L-1 .The resuscitated mycobacterium tuberculosis cells were killed by isoniazid.Conclusion The recombinant protein motif 3 had the resuscitation activity on the dormant mycobacterium tuberculosis.

Objective To investigate the effects of ciliary neurotrophic factor ( CNTF) on the expression of choline acetyltransferase ( ChAT) in hippocampus and cognitive function of diabetic mellitus ( DM) , and explore its possible mechanism .Methods 50 SD male rats were meanly divided into normal , DM, DM+DMSO, DM+CNTF, DM+CNTF+AG490 groups randomly .Streptozotocin-diabetic rats model were established , diabetic rats with depression were screened by open-field test.Escape latency and frequency of entrance into the target zone were measured by Morris water maze .The mRNA and protein levels of ChAT were examined with RT-PCR,Western blot and immuno-histochemistry.Results Compared to that of normal group ,escape latency was increased and frequency of entrance into the target zone was decreased significantly in DM,DM+DMSO and DM+CNTF+AG490 groups(P<0.05),the mRNA and protein levels of ChAT in hippocampal were decreased significantly (P<0.05) .The scores of escape latency and frequency of entrance into the target zone were highly improved in DM+CNTF group,the mRNA and protein expressions of ChAT in hippocampal were increased markedly than those of DM group (P<0.05) . Conclusions CNTF could improve cognitive ability of DM rats by increasing the mRNA and protein expressions of ChAT through the JAK 2/STAT3 pathway .

目的:探讨不同滴度的分枝杆菌噬菌体Chy3对人气道上皮细胞16HBE生长及功能的影响.方法:不同滴度噬菌体Chy3作用于人气道上皮细胞后,采用倒置显微镜观察细胞生长形态变化,CCK-8法检测细胞存活率,流式细胞仪检测细胞凋亡率,ELISA法检测细胞上清液中细胞因子分泌水平,RT-PCR和Western blot分别检测上皮细胞黏蛋白MUC5AC mRNA和蛋白表达水平.结果:不同滴度噬菌体Chy3作用于人气道上皮细胞一段时候后,细胞的生长形态、存活率、凋亡率、细胞因子分泌水平以及黏蛋白MUC5AC表达与正常组比较差异均无统计学意义(P＞0.05).结论:分枝杆菌噬菌体Chy3无论高低滴度对体外培养人气道上皮细胞生长和功能均无明显影响,有较好的安全性.

OBJECTIVE:To explore the effect of Xiaoyu anshen capsule on the space learning-memory ability of chronic stress-induced depressed model rats.METHODS:The enrolled rats were divided into 6 groups:blank control group,model group,Fluoxetine group,Xiaoyu anshen capsule groups(high,medium and low dose).Chronic depression rat model was induced by chronic stress and isolated breeding.Water-maze video tracking analysis system was used to detect the capacity of rats' space learning-memory,and the indexes such as the body weight of rats before and after modeling and the consumption of sucrose,etc.were measured.RESUTLS:Compared with the blank control group,the model control group showed significantly decreased space learning-memory capacity,significantly lower in body weight increase and significantly less sucrose solution consumption.Compared with the model control group,the high,medium and low dose Xiaoyu anshen capsule-treated group showed significantly shortened escape latency,the high and medium dose groups showed increased number of times of crossing the hidden platform and body weight increase,and in high dose Xiaoyu anshen capsale group sucrose consumption increased.CONCLUSION:Xiaoyu anshen capsule is a nootropic Chinese herbal medicine for its inhibitory effect on the abnormality in body weight and sucrose solution consumption of chronic stress-induced depressed model rats and their action in enhancing the space learning-memory ability.