Background and purpose: Cervical cancer is the most common gynecological malignancy, and human papillomavirus (HPV) is closely related to cervical cancer/precancerous lesions. Correlation between expressions of HPV16 E7 and FoxM1 was found in cervical tissue biopsy RNA samples and cervical cancer cell lines. This study aimed to discover the potential immunohistochemical markers for cervical squamous cell carcinoma (SCC) and its precursor. We conducted correlation analysis for high-risk HPV (hrHPV) infection versus nuclear protein expressions of transcriptional factor FoxM1 and its downstream target Cdc25B using uterine cervical tissues. Methods: Histological samples obtained from cervical biopsy, conization or hysterectomy were collected from pathology archives of Fudan University Shanghai Cancer Center from 2007 to 2009. Nuclear expressions of FoxM1 and Cdc25B were evaluated by immunohistochemistry and compared with P16 and Ki-67 immunostaining, as well as HPV DNA tests for 23 genotypes. Results: A total of 140 cases were recruited, including normal (n=22), cervical intraepithelial neoplasia (CIN)1 (n=28), CIN2/3 (n=50) and SCC (n=40) specimens. The positive rates of hrHPV, FoxM1, Cdc25B, P16 and Ki-67 in CIN2+ were 100.00% (90/90), 100.00% (90/90), 94.44% (85/90), 85.56% (77/90) and 97.78% (88/90) respectively, and all rates increased with the severity of the disease (Jonckheere-Terpstra test, P<0.0001). FoxM1 and Cdc25B expressions correlated with hrHPV infection, P16 and Ki-67 (Spearman’s correlation test, P<0.0001). The area under the curve (AUC) values of FoxM1 and Cdc25B for diagnosing CIN2+ were 0.850 and 0.822, respectively. Conclusion: The correlation between hrHPV infection and FoxM1 or Cdc25B protein expression in cervical squamous epithelium was confirmed. Nuclear FoxM1 and Cdc25B proteins may be potential biomarkers for CIN2+.

Objective To investigate the gene expression profiles of side population (SP) cells in human neuroblastoma SK-N-SH cells.Methods After staining the neuroblastoma SK-N-SH cell line with Hoechst 33 342 dye,the SP and nonside population (non-SP) cells were separated using flow cytometric cell sorting.The isolated SP cells from neuroblastoma SK-N-SH cell line presented several stem cell properties.The mRNA expression profiles were measured by Affymetrix GeneChip oligonucleotide array.Results Among the 53544 differentially expressed genes,3453 were significantly upregulated more than 2 folds in SP cells.Using signal interaction network analysis,we found that MAPK/ERK1 and MAPK14/p38a signaling pathways played the key role in the up-regulation of these genes.Conclusions The expressions of the gene associated with MAPK/ERK1 and MAPK14/p38a signaling pathway are up-regulated in SP cells,which may be responsible for metastasis of human neuroblastoma.

儿童门静脉畸形为一种临床少见病,类型多样,临床表现不一,存在多种伴发畸形.目前文献报道的类型根据解剖学可分为13种:肝动脉-门静脉瘘,先天性门静脉缺如,门静脉海绵样变,门静脉-肝静脉瘘,门静脉闭锁,门静脉狭窄,门静脉囊状扩张,肝外分离双门静脉,肝内双门静脉,肝内双矢状部,门静脉左、右干反位,门静脉无分叉变异,十二指肠前门静脉;根据有无门体分流可分为门体分流型门静脉畸形及无门体分流型门静脉畸形;根据病因可分为先天性和获得性:获得性门静脉畸形指由于炎症、肿瘤、外伤等导致正常门脉系统结构被破坏所致;先天性门静脉畸形则是由于门脉系统胚胎发育异常所致,多于儿童期出现症状.各类型均未形成标准化的治疗方案,主要治疗方式包括手术和介入治疗.本文对常见儿童门静脉畸形的诊治现状做一综述.

目的 报告1例腹腔镜下U形缝合腹前壁修补胸骨后疝,并对目前腹腔镜修补儿童胸骨后疝的常用手术方式、疗效、复发率等进行讨论.方法 男性患儿,8个月,因出生后反复肺部感染来院就诊,胸片及CT检查提示下胸腔多发囊状透亮影,消化道钡餐显示胸骨后疝.腹腔镜下U型缝合膈肌缺损后缘、疝囊底及腹前壁,将疝完全关闭.结果 手术时间为45min,术后1 d进食,患儿恢复良好,无术中、术后并发症.随访3个月无复发.结论 腹腔镜下U形缝合膈肌缺损后缘、疝囊底及腹前壁全层,治疗儿童胸骨后疝简单,有效,有一定的安全性.