目的：探讨高迁移率族蛋白1（HMGB 1）及人跨膜蛋白 Notch1在宫颈癌发病中的作用及相关性。方法选择子宫颈组织标本54例份，包括子宫颈鳞癌18例份（宫颈癌组）、子宫颈上皮内瘤变18例份（ CIN组）、正常子宫颈组织18例份（对照组），采用Western blot方法检测各组HMGB 1、Notch1蛋白的表达情况，采用线性相关分析法分析HMGB 1、Notch1蛋白在CIN及宫颈癌组织中的相关性。结果宫颈癌组HMGB1蛋白表达高于CIN组及对照组，CIN组HMGB1蛋白表达高于对照组（P均＜0．05）。宫颈癌组Notch1蛋白表达高于CIN组及对照组，CIN组Notch1蛋白表达高于对照组（P均＜0．05）。 CIN组HMGB1与Notch1蛋白表达呈正相关（r＝0．96，P＜0．05），宫颈癌组HMGB1与 Notch1蛋白表达呈正相关（r＝0．96，P＜0．05）。结论HMGB1、Notch1蛋白在CIN及宫颈癌组织中均表达增高，两者在CIN及宫颈癌组织中的表达呈正相关，可能共同参与宫颈癌的发病。

Objective To explore the effects of hyperthermia on the development of hamster embryonic heart .Methods Ham-sters were randomly divided into 2 groups.Experimental groups were subjected to hyperthermia (42℃in hot water for 20min) in day 8 post coitus.The embryos were obtained at 12,24,36,48h after treatment respectively,and serial sections of embryonic heart were prepared .The expressions of proteins p-JNK1/2 in the embryonic heart were observed with immunofluorescent staining technique .Results At 12,24,36 and 48 hours,expressions of p-JNK1/2 in the experimental groups were decreased ,and the 36 and 48hours were decreased significantly .Con-clusion The expressions of p-JNK1/2 decrease significantly in the embryonic heart of hamster after hyperthermia ,suggesting p-JNK1/2 may play an important role in the normal development of heart .

目的 探讨高温致神经管畸形(neural tube defects,NTD)的分子机制,为防治NTD的发生提供理论依据.方法 在高温致金黄地鼠NTD模型的基础上,应用Western blot技术和免疫荧光染色技术观察NTD发生过程中转录抑制因子(Hairy and enhancer of split homolog-1,Hes1)在鼠胚神经上皮细胞中的表达变化.结果 Western blot结果显示:Hes1蛋白在正常对照组金黄地鼠神经管中稳定表达.高温后Hes1的表达有不同程度的降低,其中高温后16h、36h(即E8.5、E9.5)与正常对照组相比差异有统计学意义(P＜0.05).Hes1免疫阳性产物主要分布于鼠胚神经管上皮细胞和周围间充质细胞的胞浆中,高温后不同时间点胚胎神经上皮细胞内的Hes1表达量较对照组减弱.其中高温处理后16h、36h(即E8.5、E9.5)差异显著(P＜o.05).结论 Hes1参与了胚胎神经管的发育过程,其表达降低在NTD的发生中起重要作用.