为研究黄芩素对氟苯尼考在大鼠体内药动学的影响,将12只大鼠随机分成单用组、合用组,合用组连续7d灌服黄芩素(50 mg/kg,1次/d),单用组则给予相同体积的生理盐水,第8d两组大鼠均按25 mg/kg剂量灌服氟苯尼考,给药后按时间点连续采样,数据采用DAS2.0分析.试验结果表明,黄芩素连续给药7d,可以增加氟苯尼考在大鼠体内的吸收,减缓代谢消除,提高口服生物利用度,提示这两种药物在临床上合用具有潜在的药动学相互作用.

为了探讨当归补血汤对蛋鸡产蛋高峰后期生产性能、抗氧化性能及蛋品质的影响,选用体重、产蛋率基本一致的42周龄罗曼粉壳蛋鸡2 160只,分为4组,每组5个重复,每个重复108只.各组均饲喂相同基础饲粮,试验组分别在饮水中添加0.2％、0.5％和1.0％的当归补血汤,试验期28 d.结果表明:①与对照组相比,0.5％和1.0％组产蛋率显著提高(P＜0.05),料蛋比降低(P＜0.05),1.0％组蛋壳强度显著增加(P＜0.05),次蛋率显著降低(P＜0.05).各组平均蛋重、蛋白高度、哈夫单位、蛋黄颜色无显著差异(P＞0.05).②与对照组相比,0.2％、0.5％和1.0％组在总抗氧化物(TAOC)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)水平上均显著提高(P＜0.05),丙二醛(MDA)水平无显著差异(P＞0.05).0.2％组血钙(Ca)和血磷(P)水平显著提升(P＜0.05),0.2％、0.5％和1.0％组血清中甘油三酯(TG)水平均极显著降低(P＜0.01),0.5％和1.0％组血清中胆固醇(TC)水平显著降低(P＜0.05).由此可知,当归补血汤添加水平为0.5％～1.0％时,能显著提高蛋鸡产蛋高峰后期产蛋率、抗氧化能力和增强蛋壳强度.

To provide a methodological reference for the study of metabolism of liver enzymes, an HPLC method for simultaneously determining three cytochrome P450(CYP) (CYP1A2, CYP2C9, CYP3A4) probe drugs in rat plasma was established.Cocktail probe solution was prepared by mixing three specific probe drugs of CYP1A2, 2C9 and 3A4(phenacetin, tolbutamide and midazolam, respectively).The three drugs were extracted with one solvent system, determined in rat plasma by HPLC, and verified the specificity, linear range, detection limit, precision, recovery and stability.The isolation was performed on a Diamonsil C18 column (4.6 mm×250 mm, 5 μm) with mobile phase consisting of methanol-50 mM phosphate buffer (45∶55;pH3.2);the detection wavelength was 230 nm;the flow rate was 1.0 mL/min and the column temperature was 35 ℃.Results: There were good linearity of phenacetin, midazolam and tolbutamide in the ranges of 0.10～10.0 μg/mL(r=0.9993), 0.05～5.0 μg/mL(r=0.9999), 0.10～50.0 μg/mL(r=0.9991).The recoveries were 79.28％～87.91％, 76.43％～85.36％, 82.79％～90.61％, respectively.The method is rapid, accurate, sensitive and suitable for simultaneously determining the concentrations of three CYP450 probe drugs(phenacetin, tolbutamide and midazolam) in rat plasma, the three drugs and the internal standard(carbamazepine) were separated well under the above HPLC conditions, which could be employed in evaluating the effects of drugs on rat's CYP450 subtype enzymes(CYP1A2, CYP2C9, CYP3A4) activities and in relatived studies.

为研究冰片对氟苯尼考血浆蛋白结合率的影响,采用超滤法结合超高效液相色谱法,比较未加冰片和加入冰片后氟苯尼考的血浆蛋白结合率.25、5 、1 μg/mL三种浓度下,氟苯尼考血浆蛋白结合率分别为牛23.68%±1.25%、25.26%±1.42%、26.46%±1.48%,大鼠31.33%±1.82%、32.32%±1.46%、34.15%±1.59%,家兔15.69% ±1.45%、19.31%±1.26%、24.38%±1.51%,肉鸡21.68% ±1.37%、20.13%±1.84%、20.52%±1.77%,猪30.08%±1.03%、31.02%±1.28%、30.56%±1.55%.氟苯尼考5 μg/mL浓度下与冰片合用,血浆蛋白结合率无显著变化.结果表明,氟苯尼考有较低的血浆蛋白结合率,冰片对氟苯尼考血浆蛋白结合率无显著影响.

To study the effect of licorice extract on the pharmacokinetics and tissue distribution of levofloxacin in rats.The SD rats were randomly divided into the single group and the combined group,that were administrated respectively with normal saline and extract of licorice.After pretreated with extract of licorice (0.5 g/kg,qd)for 7 d,levofloxacin was given to the rats by intragastric administration at the dose of 20 mg/kg.The concentrations of levofloxacin in plasma and tissue after administration were determined by HPLC.Pharmacokinetic data were calculated by DAS 2.0.Comparision with the single administration group,the main pharmacokinetic parameters (t1 /2 ,tmax ,MRT0 -36h ,MRT0 -∞)were significantly increased (P <0.01),the concentration level of levofloxacin in tissuet showed a feature of first decreasing followed by increasing,but the characteristic of tissue distribution was not changed.The metabolic characteristics of levofloxacin were influenced by licorice extract in rats significantly,including decreasing the speed of absorption,retarding the speed of elimination,accelerating the distribution from blood to tissue and lengthening the time of drug action.

将20只鸡随机分成对照组和试验组,对照组单剂量灌胃给予左氧氟沙星(levofloxacin,LVLX) 10 mg/kg;试验组左氧氟沙星10 mg/kg与甘草提取物0.3 g/kg合用.采用HPLC法测定血药质量浓度,并以DAS2.0软件计算并比较主要药动学参数.结果显示:对照组和试验组左氧氟沙星的主要药动学参数Cmax分别为(1.415±0.116)、(1.321±0.165)mg/L,tmax分别为(1.983±0.172)、(2.365±0.325)h,AUC0～36 h分别为(8.659±0.852)、(9.415±1.176)mg·L-1·h-1,t12分别为(3.714±0.674)、(8.341±0.853)h,MRT0～36 h分别为(4.116±0.238)、(6.648±0.676)h,Vz/F分别为(3.154±0.296)、(3.315±0.436)L/kg.与对照组相比,试验组药动学参数Cmax降低,AUC0～36 h、Vz/F增大,但差异均不显著(P＞0.05);tmax、t12、MRT0～36 h均极显著增大(P＜0.01).结果表明:合用甘草提取物和左氧氟沙星,甘草提取物能减慢左氧氟沙星在体内的吸收和消除速度,延长药物在体内的作用时间.

Dose of ABP（400 mg/kg） was given to 7-day-old chicken,and taken blood from jugular vein at 14th day,21st day and 28th day.The content of sera antibody（IgG,IgA,IgM） and blood biochemical parameters（glutamate-pyruvate transaminase,glutarmic-oxatoacetic transaminase,alkaline phosphatase,calcium,albumin） were measured.The result showed that ABP（400 mg/kg） added group,compared with the control group,its IgM,IgG and IgA increased significantly（0.01P≤0.05）,but the albumin,glutarmic-oxatoacetic transaminase,glutamate-pyruvate transaminase,alkaline phosphatase,calcium had no significant difference.It indicated that ABP could elevate serum level to improve the immune ability of chicken,and all of the blood biochemical parameters were not affected significantly,So ABP could be used as a feed additives in animal and poultry industry.