Background: Aurora B is one of the spindle assembly checkpoint proteins. It plays a crucial role in the regulation of mammalian female mitosis and meiosis. In this study, we examined the mechanism of small ubiquitin-related modifier modification (SUMOylation) of Aurora B on the chromosome arrangement of mice oocytes. Methods: Oocytes were randomly divided into three groups: Control group, AuroraB_K207R group, and the small ubiquitin-related modifier (SUMO) inhibitor group. The number of oocytes that developed at 2.5, 8, and 14 h were counted. Metaphase II (MII) oocytes in each group were selected for observing the arrangement of chromosomes and spindles. Meanwhile, MII oocytes in the control and AuroraB_K207R groups were collected for use in the Smart-seq2 protocol. Results: Messenger ribonucleic acid (mRNA) was obtained from the AuroraB_K207R group via the transcription of a linearized pMD 18T-T7-AuroraB_K207R plasmid. The proportion of metaphase I (MI) oocytes after 8 h in the SUMO inhibitor group was significantly lower than in the AuroraB_K207R and control groups (p < 0.05). The MII oocytes in the AuroraB_K207R and SUMO inhibitor groups decreased significantly compared with the control group after 14 h (p < 0.05). Furthermore, the rate of abnormal chromosome arrangement in the AuroraB_K207R and SUMO inhibitor groups increased significantly compared with the control group (p < 0.05). Using Smart-seq2, a total of 3288 differentially expressed genes were identified; 2874 of these genes involving Aurora B were upregulated and 414 genes were downregulated in the AuroraB_K207R group. Conclusions: Small ubiquitin-related modifier modifications may directly affect the regulatory function of Aurora B on chromosome arrangement in mice oocyte meiosis, leading to oocyte maturation disorder. Therefore, the SUMOylation of Aurora B plays an important role in chromosome arrangement in mice oocyte meiosis.

目的 探讨时差成像系统对复苏周期单囊胚移植妊娠结局的影响.方法 选取在本中心接受体外受精(IVF)或卵胞浆内单精子注射(ICSI)治疗的 113 个周期,将每周期获得的受精卵随机分配到时差成像系统(实验A组)和桌面培养箱(对照B组)进行卵裂期胚胎培养,囊胚培养全部转入桌面培养箱进行,同一培养系统胚胎同一液滴集合培养,按照受精方式和年龄进行分层,比较两组2PN卵裂率、D3 优质胚胎率、囊胚形成率、可利用囊胚形成率、优质囊胚形成率.将集合培养形成的可用囊胚进行冻存,并对第一次复苏周期囊胚移植的妊娠结局进行分析.复苏囊胚来源于时差成像系统的为实验C组(42 周期),来源于桌面培养箱的为对照D组(33 周期).结果 实验A组和对照B组患者的2PN卵裂率、D3 优质胚胎率、囊胚形成率、可利用囊胚形成率、优质囊胚形成率比较,差异无统计学意义.实验C组和对照D组患者的年龄、转化日内膜厚度、流产率比较,差异无统计学意义;但实验C组的临床妊娠率(83.33%vs 60.61%)和着床率(83.33%vs 60.61%)显著高于对照D组(P＜0.05).结论 时差成像系统对胚胎发育没有明显不利影响,可能改善复苏周期囊胚移植的临床妊娠结局.

目的 阐明Wnt/β-catenin通路对Foxo3a及其下游分子的调控在多囊卵巢综合(PCOS)征患者颗粒细胞中的作用机制.方法 选取在接受体外受精-胚胎移植(IVF-ET)或者卵胞浆内单精子显微注射(ICSI)助孕治疗的PCOS患者及单纯输卵管因素患者为研究对象.分离、纯化患者取卵日卵泡液中的卵巢颗粒细胞,采用实时定量PCR方法检测颗粒细胞中β-catenin、Foxo3a、P27、Cyclin D1、Caspase3、Caspase8的表达水平.结果 与对照组相比,PCOS患者卵巢颗粒细胞中β-catenin、Foxo3a、P27、Caspase3、Caspase8表达水平升高,P<0.05或<0.01,而两组患者卵巢颗粒细胞中的Cy-clin D1表达水平的差异无统计学意义.结论 PCOS患者卵巢颗粒细胞的凋亡增加,增殖能力下降.Wnt/β-catenin通路对Foxo3a及其下游信号分子的调控参与了PCOS患者卵巢颗粒细胞异常的机制.

目的 探讨染色体复杂平衡易位对女性受孕的影响.方法 应用G显带技术对1对夫妇患者的外周血染色体进行分析,通过体外受精-胚胎移植(IVF-ET)技术对患者胚胎发育情况进行分析.结果 患者外周血染色体核型为46,XX,t(5;10;13;14),4条染色体发生复杂易位,同时存在插入,丈夫外周血染色体核型正常;该夫妇进行1周期IVF助孕,获卵14枚,受精12枚,第3日(D3)形成优质胚胎11枚,第5日(D5)形成优质囊胚7枚.结论 本患者虽然染色体复杂易位情况罕见,但仍能获得较多的优质囊胚.罕见的染色体复杂平衡易位患者获得完全正常后代的可能性极低,建议通过供卵途径助孕.

慢性前列腺炎是较为常见的男科疾病,患病率在10%左右 [1] ,据统计约50%的男性在一生中的不同时期会出现前列腺炎相关的症状 [2] 。研究表明,慢性前列腺炎与男性不育症关系密切,其中Ⅲ型前列腺炎（慢性前列腺炎/慢性骨盆疼痛综合症）是其

目的:探讨多囊卵巢综合征(PCOS)患者不同时期血浆Nesfatin-1水平的变化.方法:选择健康育龄妇女33例和PCOS不孕患者30例,于经期2～3天时期采集前臂静脉血,ELISA方法测定血浆Nesfatin-1,常规方法测定性激素进行比较；另选择20例PCOS患者口服避孕药达英35加二甲双胍调节月经周期两周期,与用药前进行自身血浆Nesfatin-1水平比较,观察有无变化.结果:PCOS患者血浆Nesfatin-1水平低于健康妇女,血LH、T水平高于健康妇女,差异有统计学意义(P＜0.05),余性激素差异无统计学意义(P＞0.05)；PCOS患者用药后血浆Nesfatin-1水平高于用药前,差异有统计学意义(P＜0.05).结论:多囊卵巢综合征患者血浆Nesfatin-1低于健康妇女,口服达英-35加二甲双胍两周期后患者血浆Nesfatin-1水平上升.