Background: Aurora B is one of the spindle assembly checkpoint proteins. It plays a crucial role in the regulation of mammalian female mitosis and meiosis. In this study, we examined the mechanism of small ubiquitin-related modifier modification (SUMOylation) of Aurora B on the chromosome arrangement of mice oocytes. Methods: Oocytes were randomly divided into three groups: Control group, AuroraB_K207R group, and the small ubiquitin-related modifier (SUMO) inhibitor group. The number of oocytes that developed at 2.5, 8, and 14 h were counted. Metaphase II (MII) oocytes in each group were selected for observing the arrangement of chromosomes and spindles. Meanwhile, MII oocytes in the control and AuroraB_K207R groups were collected for use in the Smart-seq2 protocol. Results: Messenger ribonucleic acid (mRNA) was obtained from the AuroraB_K207R group via the transcription of a linearized pMD 18T-T7-AuroraB_K207R plasmid. The proportion of metaphase I (MI) oocytes after 8 h in the SUMO inhibitor group was significantly lower than in the AuroraB_K207R and control groups (p < 0.05). The MII oocytes in the AuroraB_K207R and SUMO inhibitor groups decreased significantly compared with the control group after 14 h (p < 0.05). Furthermore, the rate of abnormal chromosome arrangement in the AuroraB_K207R and SUMO inhibitor groups increased significantly compared with the control group (p < 0.05). Using Smart-seq2, a total of 3288 differentially expressed genes were identified; 2874 of these genes involving Aurora B were upregulated and 414 genes were downregulated in the AuroraB_K207R group. Conclusions: Small ubiquitin-related modifier modifications may directly affect the regulatory function of Aurora B on chromosome arrangement in mice oocyte meiosis, leading to oocyte maturation disorder. Therefore, the SUMOylation of Aurora B plays an important role in chromosome arrangement in mice oocyte meiosis.

目的 探讨时差成像系统对复苏周期单囊胚移植妊娠结局的影响.方法 选取在本中心接受体外受精(IVF)或卵胞浆内单精子注射(ICSI)治疗的 113 个周期,将每周期获得的受精卵随机分配到时差成像系统(实验A组)和桌面培养箱(对照B组)进行卵裂期胚胎培养,囊胚培养全部转入桌面培养箱进行,同一培养系统胚胎同一液滴集合培养,按照受精方式和年龄进行分层,比较两组2PN卵裂率、D3 优质胚胎率、囊胚形成率、可利用囊胚形成率、优质囊胚形成率.将集合培养形成的可用囊胚进行冻存,并对第一次复苏周期囊胚移植的妊娠结局进行分析.复苏囊胚来源于时差成像系统的为实验C组(42 周期),来源于桌面培养箱的为对照D组(33 周期).结果 实验A组和对照B组患者的2PN卵裂率、D3 优质胚胎率、囊胚形成率、可利用囊胚形成率、优质囊胚形成率比较,差异无统计学意义.实验C组和对照D组患者的年龄、转化日内膜厚度、流产率比较,差异无统计学意义;但实验C组的临床妊娠率(83.33%vs 60.61%)和着床率(83.33%vs 60.61%)显著高于对照D组(P＜0.05).结论 时差成像系统对胚胎发育没有明显不利影响,可能改善复苏周期囊胚移植的临床妊娠结局.

Adenomyosis is a diffuse or localized organic disease caused by benign invasion of endometrial glands and stroma into the myometrium. It is a common disease that seriously affects reproductive health of women in childbearing age. Due to the unknown etiology and pathophysiological mechanism, and the lack of unified diagnostic criteria and effective treatment methods, total or subtotal hysterectomy has become a radical treatment for adenomyosis, which will lead to the complete loss of fertility. With the continuous exploration of the treatment to adenomyotic patients who have infertility or fertility intentions, new drugs, surgical methods and treating concepts appears. Adopt individualized conservative therapeutic strategies for patients with different conditions, preserve the uterus as much as possible and protect the patient’s fertility, which will play an important role on the follow-up assisted reproductive treatment and long-term management of adenomyosis.

与ABO 系统不同,Rh血型系统是红细胞血型中最复杂的一个系统,而Rh缺失型D--则是该血型系统中一种极为罕见的变异体,血清学表现为D抗原的增强和其他抗原(C、c、E、e)抗原的缺失.本例新生儿因发生溶血病,多次交叉配血失败,经鉴定其母亲为 Rh缺失型D--,现报道如下.

目的 阐明Wnt/β-catenin通路对Foxo3a及其下游分子的调控在多囊卵巢综合(PCOS)征患者颗粒细胞中的作用机制.方法 选取在接受体外受精-胚胎移植(IVF-ET)或者卵胞浆内单精子显微注射(ICSI)助孕治疗的PCOS患者及单纯输卵管因素患者为研究对象.分离、纯化患者取卵日卵泡液中的卵巢颗粒细胞,采用实时定量PCR方法检测颗粒细胞中β-catenin、Foxo3a、P27、Cyclin D1、Caspase3、Caspase8的表达水平.结果 与对照组相比,PCOS患者卵巢颗粒细胞中β-catenin、Foxo3a、P27、Caspase3、Caspase8表达水平升高,P<0.05或<0.01,而两组患者卵巢颗粒细胞中的Cy-clin D1表达水平的差异无统计学意义.结论 PCOS患者卵巢颗粒细胞的凋亡增加,增殖能力下降.Wnt/β-catenin通路对Foxo3a及其下游信号分子的调控参与了PCOS患者卵巢颗粒细胞异常的机制.

Objective The present study aims to evaluate the effect of monosodium glutamate on testicular spermatogenesis in mice from the perspective of the hypothalamic-pituitary-testicular axis and whether this destructive effect is alleviated with time. Methods Neonatal mice were randomly divided into a monosodium glutamate (MSG) group and a control group, just below the interscapular region after birth with 10 µL MSG to deliver 4 mg/g (body mass), or with equivalent volumes of 0.9% saline. Samples which involved blood, brains and testicles of mice were collected and measured at puberty at 60 days and adulthood at 90 days. Results The results show that the fluorescence intensity of GnRH nerve fibers, the levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) hormones in the reproductive system, the number of spermatocytes and spermatozoa in testicular sections, the body length, body weight, testicular weight, and testicular index in the 60-day-old mice in monosodium glutamate group (MSG60 group) and the MSG90 group were lower than those in the 60-day-old mice in normal control group (NC60 group) (p < 0.05), but the number of apoptotic cells in the testicular section was higher than in the NC60 group (p < 0.05). When the 90-day-old mice in monosodium glutamate group (MSG90 group) was compared with the MSG60 group, except for body weight and testicular weight increase (p < 0.05), there is no significant difference in the other parameters mentioned above (p > 0.05). Conclusion Monosodium glutamate can cause reproductive toxicity to male mice by damaging GnRH neurons, and this reproductive toxicity cannot be relieved spontaneously over time. These findings are supported by observed histological changes.

目的 探讨染色体复杂平衡易位对女性受孕的影响.方法 应用G显带技术对1对夫妇患者的外周血染色体进行分析,通过体外受精-胚胎移植(IVF-ET)技术对患者胚胎发育情况进行分析.结果 患者外周血染色体核型为46,XX,t(5;10;13;14),4条染色体发生复杂易位,同时存在插入,丈夫外周血染色体核型正常;该夫妇进行1周期IVF助孕,获卵14枚,受精12枚,第3日(D3)形成优质胚胎11枚,第5日(D5)形成优质囊胚7枚.结论 本患者虽然染色体复杂易位情况罕见,但仍能获得较多的优质囊胚.罕见的染色体复杂平衡易位患者获得完全正常后代的可能性极低,建议通过供卵途径助孕.