Background: We explored the effects of repeated plateletpheresis on the platelet P-selectin expression and soluble P-selectin (sP-selectin) concentrations in platelet donors.Methods: Totally 289 platelet donors and 97 first-time whole blood (WB) donors were enrolled from the blood donor registry at the Fujian provincial blood center, China. The accumulative numbers of plateletpheresis in the last 2 y for participants were recorded, and the basal concentrations of platelet count, sP-selectin and total platelet P-selectin (pP-selectin) were determined.Results: Platelet donors had significantly higher basal concentrations of sP-selectin compared to WB donors (24.12 +/- 7.33 ng/mL vs. 20.74 +/- 5.44 ng/mL, P < 0.0001), with no difference in platelet count and pP-selectin concentrations. Increased numbers of platelet donation were correlated with a steady increase of sP-selectin (r = 0.18, P = 0.002). Multivariate regression analysis identified that the frequency of plateletpheresis is an independent factor for the rise of the sP-selectin concentration (t = 2.64, P = 0.009) while no association was found for pP-selectin and platelet count.Conclusions: Repeated plateletpheresis could result in an increased basal concentration of sP-selectin in blood donors whereas not an alteration in the concentrations of total platelet P-selectin. It remains to be determined whether this might be a consequence of endothelial activation or platelet activation or some other phenomenon. (C) 2016 Elsevier B.V. All rights reserved.

BACKGROUND Recent genome-wide association studies in Caucasians suggested that an association exists between the ABO gene locus and soluble levels of P-selectin (sP-selectin). However, it is unclear if the relationship corresponds to the phenotypic expression of ABO groups or is present in different ethnic groups. The aim of this study was to verify this observation at both genotypic and phenotypic levels in a healthy Chinese population.STUDY DESIGN AND METHODS The ABO blood groups were determined by both phenotypes and genotypes in 440 healthy Chinese Han volunteers, while P-selectin levels were evaluated for sP-selectin and total platelet P-selectin (pP-selectin).RESULTS ABO phenotyping and quantitative analysis of individual sP-selectin plasma levels were combined to demonstrate that individuals phenotypically expressing the A antigen have approximately 20% lower sP-selectin plasma levels than those carrying the B or O phenotype (p<0.0001), but that no difference exists between A and AB and between B and O phenotypes. Genotyping data revealed that the presence of the A gene could be attributed to the observed difference in phenotype comparison, with no difference between A/A, A/B, and A/O genotypes. There were also no associations between ABO blood groups, either phenotypes or genotypes, and pP-selectin levels.CONCLUSION This study demonstrated an association between sP-selectin levels and ABO groups in a Chinese Han population, implicating its generalizability to other ethnic groups. This finding will improve the understanding of the mechanism of ABO blood group-associated diseases.

Objective To study the distribution characteristics and regularity of ABO blood group genotype of Han population in Fuzhou.Methods From 12 January 2011 to 23 May 2011,a total of 450 Han blood donors who donated blood in Fujian Provincial Blood Center were selected in the study randomly.ABO genotype of all the donors was analyzed by polymerase chain reaction-sequence specific primer (PCR-SSP).Results In 450 unrelated individuals of Fuzhou Han population,five alleles of A1 (21.78％),A205 (0.67％),B (19.33％),O1 (32.78％),O2 (25.44％) were detected,but no O3 allele was discovered; twelve genotypes were found,consisting of A1O1 (14.00％),A1O2 (11.11％),A1A1 (4.22％),A1A205 (1.11％),BB (3.56％),BO1(12.00％),BO2 (10.44％),O1O1 (10.44％),O1O2 (18.67％),O2O2 (5.33％),A1B (8.89％),A205B (0.22％).The difference was not statistically significant between observed value and expected value in different genotypes (x2 =13.5339,P＞0.05).The genotype frequency distribution of this group was in good agreement with Hardy-Weinberg equilibrium.Conclusions Corresponding with ABO genotype,the distribution characteristics of ABO blood group in Fuzhou Han population was O＞A＞B＞AB,the ABO gene frequency distributions from high to low were O1,O2,A1,B,A205.A2 allele had its own characteristic,dominated by A205. Key words: Han population; ABO blood group; PCR-SSP; Genotype