目的 分析福州地区2014—2020年单采血小板献血者输血相关传染病标志物筛查不合格情况的变化,探讨降低筛查不合格率的措施,减少单采血小板的报废.方法 回顾性分析2014—2020年福州地区60735例单采血小板献血者的血液筛查结果,对不合格指标进行分析.结果 福州地区单采血小板献血者输血相关传染病标志物筛查不合格率为0.73％,各年度筛查总不合格率比较差异有统计学意义(P<0.05),丙氨酸氨基转移酶(ALT)和核酸扩增检测技术(NAT)是主要不合格项;不同性别和不同年龄段筛查总不合格率比较差异均有统计学意义(P<0.05),全血献血者的筛查总不合格率高于单采血小板献血者(P<0.05).结论 应规范献血前的健康咨询和体检初筛工作,加强试剂和设备管理,做好献血者的屏蔽与归队工作,减少血液的报废,防止不必要的血源流失.

目的 了解核酸检测技术在福州地区无偿献血者血液筛查中的应用情况,评估开展血液核酸检测的必要性和试剂升级前后的检测性能差异.方法 应用Procleix TIGRIS全自动核酸检测系统,对无偿献血者血液标本进行HBV/HCV/HIV-1联合检测,同时采用2种血清学ELISA试剂对所有标本进行HBsAg、抗-HCV、抗-HIV/P24和抗-TP等项目并行检测,并对核酸联合筛查反应性的标本进行鉴别试验.结果 2012年～2017年,共检测献血者血液标本491159份,其中ELISA无反应性,NAT反应性标本共计1637份,检出HBV窗口期标本共477份,HIV窗口期标本2份.2015年核酸检测升级后,ELISA无反应性NAT反应性率由0.28％提高到0.41％,鉴别阳性的预测值由25.61％提高到33.89％.结论 核酸检测技术应用于无偿献血者血液筛查能有效降低输血感染风险,保证临床用血安全,试剂的更新升级有助于检测性能提高.

Background: We explored the effects of repeated plateletpheresis on the platelet P-selectin expression and soluble P-selectin (sP-selectin) concentrations in platelet donors.Methods: Totally 289 platelet donors and 97 first-time whole blood (WB) donors were enrolled from the blood donor registry at the Fujian provincial blood center, China. The accumulative numbers of plateletpheresis in the last 2 y for participants were recorded, and the basal concentrations of platelet count, sP-selectin and total platelet P-selectin (pP-selectin) were determined.Results: Platelet donors had significantly higher basal concentrations of sP-selectin compared to WB donors (24.12 +/- 7.33 ng/mL vs. 20.74 +/- 5.44 ng/mL, P < 0.0001), with no difference in platelet count and pP-selectin concentrations. Increased numbers of platelet donation were correlated with a steady increase of sP-selectin (r = 0.18, P = 0.002). Multivariate regression analysis identified that the frequency of plateletpheresis is an independent factor for the rise of the sP-selectin concentration (t = 2.64, P = 0.009) while no association was found for pP-selectin and platelet count.Conclusions: Repeated plateletpheresis could result in an increased basal concentration of sP-selectin in blood donors whereas not an alteration in the concentrations of total platelet P-selectin. It remains to be determined whether this might be a consequence of endothelial activation or platelet activation or some other phenomenon. (C) 2016 Elsevier B.V. All rights reserved.

BACKGROUND Recent genome-wide association studies in Caucasians suggested that an association exists between the ABO gene locus and soluble levels of P-selectin (sP-selectin). However, it is unclear if the relationship corresponds to the phenotypic expression of ABO groups or is present in different ethnic groups. The aim of this study was to verify this observation at both genotypic and phenotypic levels in a healthy Chinese population.STUDY DESIGN AND METHODS The ABO blood groups were determined by both phenotypes and genotypes in 440 healthy Chinese Han volunteers, while P-selectin levels were evaluated for sP-selectin and total platelet P-selectin (pP-selectin).RESULTS ABO phenotyping and quantitative analysis of individual sP-selectin plasma levels were combined to demonstrate that individuals phenotypically expressing the A antigen have approximately 20% lower sP-selectin plasma levels than those carrying the B or O phenotype (p<0.0001), but that no difference exists between A and AB and between B and O phenotypes. Genotyping data revealed that the presence of the A gene could be attributed to the observed difference in phenotype comparison, with no difference between A/A, A/B, and A/O genotypes. There were also no associations between ABO blood groups, either phenotypes or genotypes, and pP-selectin levels.CONCLUSION This study demonstrated an association between sP-selectin levels and ABO groups in a Chinese Han population, implicating its generalizability to other ethnic groups. This finding will improve the understanding of the mechanism of ABO blood group-associated diseases.

Objective To study the distribution characteristics and regularity of ABO blood group genotype of Han population in Fuzhou.Methods From 12 January 2011 to 23 May 2011,a total of 450 Han blood donors who donated blood in Fujian Provincial Blood Center were selected in the study randomly.ABO genotype of all the donors was analyzed by polymerase chain reaction-sequence specific primer (PCR-SSP).Results In 450 unrelated individuals of Fuzhou Han population,five alleles of A1 (21.78％),A205 (0.67％),B (19.33％),O1 (32.78％),O2 (25.44％) were detected,but no O3 allele was discovered; twelve genotypes were found,consisting of A1O1 (14.00％),A1O2 (11.11％),A1A1 (4.22％),A1A205 (1.11％),BB (3.56％),BO1(12.00％),BO2 (10.44％),O1O1 (10.44％),O1O2 (18.67％),O2O2 (5.33％),A1B (8.89％),A205B (0.22％).The difference was not statistically significant between observed value and expected value in different genotypes (x2 =13.5339,P＞0.05).The genotype frequency distribution of this group was in good agreement with Hardy-Weinberg equilibrium.Conclusions Corresponding with ABO genotype,the distribution characteristics of ABO blood group in Fuzhou Han population was O＞A＞B＞AB,the ABO gene frequency distributions from high to low were O1,O2,A1,B,A205.A2 allele had its own characteristic,dominated by A205. Key words: Han population; ABO blood group; PCR-SSP; Genotype

目的 比较ELISA一步法和两步法检测HBsAg的差异.方法 经HBsAg中和试验确证的115份阳性样本和30份阴性样本,采用ELISA一步法试剂和两步法试剂进行检测.通过检测结果的统计分析评价两种试剂的检测能力.结果 115份阳性样本,一步法阳性检出率68.7%,两步法阳性检出率76.5%;30份阴性样本,一步法阴性检出率93.3%,两步法阴性检出率96.7%;对等评估指标比较结果,两步法均优于一步法,其中敏感性差异有统计学意义(P<0.01),特异性差异无统计学意义(P>0.05);阳性样本检测值S/CO,两步法高于一步法(P<0.001);阴性样本检测值(S/CO),两试剂检测差异无统计学意义(P>0.10).结论 ELISA两步法检测HBsAg的能力高于ELISA一步法,且能确保血液安全.

五常法即常组织、常整顿、常清洁、常规范、常自律,简称为5S,起源于日本,是用来维持品质环境的一种管理技术[1],是现场管理的基础,是全面质量管理(TQM)的第一步,是ISO9000有效推行的保证[2],目前已被世界上很多知名企业所采用.大量的实践证明:五常法在对生产、技术、经营活动的程序化、法制化、标准化,提高职工的素质和工作责任感,改进产品与服务,最终在顾客中建立良好声誉等方面,均有促进作用[3].

[目的] 探讨无偿献血模式下梅毒抗体筛查方法的选择. [方法] 比较福州市13 248名无偿献血者梅毒ELISA(双抗原夹心)法与TRUST法的检测结果,并与TPPA结果对照. [结果] 梅毒抗体阳性97例,其中TRUST法检出率0.23%(31例),ELISA法0.68%(90例). [结论] TRUST法检出率低于ELISA法,无法满足安全血液的要求,不宜作为无偿献血常规筛查方法.

为减少输血感染HBV的风险,血站对HBsAg检测试剂除注重特异性外,更关注灵敏度.部分血站则采用了检测成本更高的进口试剂.为了解本省常用的HBsAg ELISA试剂的整体情况,笔者收集了5个试剂厂的产品(3家进口,2家国产)进行灵敏度的比较.