Background: An increasing number of studies demonstrate that abnormal miRNA expression contributes to the advancement of many tumors. Nonetheless, the potential role of miR-125b in multiple myeloma (MM) remains unknown. Objectives: To explore the potential effects and mechanism of miR-125b in MM. Methods: Realtime quantitative PCR was used to measure the expression levels of miR-125b and MKNK2 in a variety of MM samples. Colony formation and cell counting Kit-8 (CCK-8) assays were used to assess cell proliferation, the transwell assay was used to evaluate the cell invasion capability, and dual luciferase reporter gene assay and Western blot were used to examine the interaction between miR-125b and MKNK2. Results: The levels of miR-125b were higher in MM tissue samples, alongside increased expression of MKNK2. There was a negative correlation between MKNK2 and miR-125b expression in MM tissues. MKNK2 was identified as a direct target gene of miR-125b in MM cells. Overexpression of miR-125b suppressed MM cell growth, colony formation, and invasion. In addition, MKNK2 was found to mediate the effects of miR-125b on cell proliferation, colony formation, and invasion in MM. Conclusions: miR-125b acts as a suppressive factor in multiple myeloma and can affect the malignant behavior of MM by regulating the expression of MKNK2.

目的 观察经桡动脉途径(TRA)与经股动脉(TFA)途径行肝癌经导管动脉化疗栓塞术(tace)的临床疗效.方法 选取我院行肝癌tace术患者154例,随机分为TRA组(经桡动脉路径穿刺)和TFA组(经股动脉路径穿刺)各77例,分析两组患者治疗后疗效.结果 两组患者穿刺成功率、治疗成功率、曝光时间、曝光剂量及造影剂量组间比较差异无统计学意义(P＞0.05),患者偏好比较TRA组明显高于TFA组(P＜0.05);两组治疗总有效率比较差异不具统计学意义(P＞0.05);组间比较并发症发生率比较差异不具统计学意义(P＞0.05).结论 行肝癌tace术治疗时选择TRA或TFA穿刺无明显效果差异,但患者更易接受TRA.

Objective To explore the change of miRNA-16(miR-16) level in the patients with multiple my-eloma(MM ) and its relationship with the Mayo risk stratification and prognosis judgment .Methods Each 10 mL of bone marrow samples was collected from 31 cases of MM in the hematology department of the First Af-filiated Hospital of Guangxi Medical University from August 2016 to January 2017 ,and each 5 mL of bone marrow sample was collected from 5 healthy bone marrow donors as the healthy control .The real time fluores-cence quantitative PCR(qRT-PCR) was used to analyze relative the change of miR-16 relative expression level and fluorescence in situ hybridization(iFISH) was used to detect the abnormal gene .Then the relationship be-tween miR-16 relative expression level with Mayo based risk stratification ,and the difference of miR-16 rela-tive expression level between before and after self treatment were statistically analyzed .Results The miR-16 relative expression level in MM patients was increased and positively correlated with the Mayo risk stratifica-tion .Meanwhile in the MM patients with disease progression ,the miR-16 expression was increased . Conclusion miR-16may serve as the indicator for judging the recurrence or progression of MM ,and guides the clinical personalized therapy of MM according to its expression level change .

目的:分析多发性骨髓瘤(multiple myeloma,MM)的不同患者体内miR-15a表达水平差异及其与Mayo危险分层的临床意义,判断miR-15a是否可对MM患者进行预后判断及病程进展监控,指导临床治疗方案执行.方法:通过qRT-PCR分析就诊于广西医科大学附属第一医院30例MM患者和5例正常骨髓捐献者骨髓单个核细胞内的miR-15a表达水平;同时以荧光原位杂交技术(fluorescence in situ hybridization,FISH)检测的常见异常基因为基础进行Mayo危险分层.统计学分析二者之间的相关性.结果:复发的MM标危患者miR-15a表达水平高于标危的初治患者,具有统计学意义.结论:miR-15a表达量与Mayo危险分层不具有统计学意义(P>0.05),但miR-15a的表达量与多发性骨髓瘤病程进展成正相关(P=0.0014<0.05),有望成为监测MM复发、 恶化的新生物学标志物.

目的:探讨SPARC和Tiam1基因在人早幼粒细胞白血病细胞株(HL-60)与人慢性粒细胞白血病细胞株(K562)中的表达的水平及其意义.方法:常规细胞培养,取在对数生长期的HL-60、K562细胞进行mRNA抽提;以HUVEC细胞的mRNA作为对照组,逆转录后采用实时荧光定量PCR(RTFQ-PCR)法检测SPARC和Tiam1在HL-60、K562细胞中mRNA的表达水平.结果:①SPARC和Tiam1在HL-60、K562细胞中均为低表达;②SPARC和Tiam1在HL-60、K562细胞中的表达差异均有统计学意义(P＜0.05);③SPARC与Tiam1在HL-60细胞中的表达呈负相关(r=-0.886,P＜0.01);SPARC与Ti-am1在K562细胞中的表达无相关关系(r=-0.086,P=0.872).结论:在髓系白血病中,评价SPARC与Tiam1的表达水平可能具有一定的预后价值;在急性早幼粒细胞白血病中,SPARC和Tiam1可能通过共同的信号通路调控下游基因.

Objective To investigate the significance of fluorescence in situ hybridization ( FISH) applied to the diagnosis of abnormalities in chromosome 7 in myelodysplastic syndromes ( MDS ) .Methods The bone marrow samples collected from 84 MDS patients were analyzed for chromosomal karyotypes by interphase FISH and convention -al cytogenetic analysis(CCA),respectively.A comparison was done for the detection relevance ratio of abnormalities in chromosome 7 between FISH and CCA .Results Among 84 MDS patients,35 with chromosome aberrations were accounted for 41 .7%( 35/84 ) , in which 9 cases of numerical abnormality of chromosomes , accounted for 25 .7%(9/35),were dominated by +8;Thirteen cases of chromosomal structural abnormality ,accounted for 37.1%(13/35),were dominated by 7q-,5q-and 20q-;There were 13 cases of structural and numerical abnormality ,accoun-ted for 37.1%(13/35).The most common chromosomal aberration was abnormality in chromosome +8(11 cases), the follows as complex chromosomal aberrations ( 15 cases ) , abnormality in chromosome 7 ( 9 cases ) , abnormality in chromosome 5q-(5 cases) ,abnormality in chromosome 20q-(5 cases),abnormality in chromosome Y (2 cases). Nine cases of abnormality in chromosome 7,accounted for 10.7%(9/84),were confirmed by FISH,while only 1 case of abnormality in chromosome 7,accounted for 1.2% (1/84),was confirmed by CCA (P <0.01).Conclusion FISH has a higher detection relevance ratio of abnormality in chromosome 7 in MDS patients compared with CCA ,but FISH can′t take the place of CCA .