The innovation of cancer immunotherapy is improving the prognosis of colorectal cancer (CRC) in clinics. Nevertheless, due to tumor heterogeneity and complex underlying inhibitory mechanisms, the therapeutic response greatly varies among different patients. To optimize the clinical management of CRC patients, it is critical to develop novel approaches for response monitoring and prediction. In the current study, we developed a novel near-infrared fluorescence (NIRF) imaging probe (Cy5.5-ICOS mAb) targeting the inducible T-cell costimulatory receptor (ICOS or CD278) and assessed its capacity for the detection of ICOS+-activated T cells in vivo. ICOS expression was evaluated by flow cytometry and immunofluorescence staining in subcutaneous MC38 models treated with the stimulator of interferon genes (STING) agonist (STINGa). NIRF imaging study was performed 1 day after the last treatment, and tumor volume was monitored every other day with a caliper. A significantly higher optical signal could be detected at tumor regions in STINGa group, compared with that in the PBS group at all time points imaged, and this was in line with ex vivo imaging and immunofluorescence staining study. The data demonstrated that Cy5.5-ICOS mAb could detect ICOS+-activated T cells with high specificity, and ICOS NIRF imaging is a promising strategy for predicting and monitoring immune response in CRC.

回顾性分析2011年7月至2019年10月于哈尔滨医科大学附属第一医院胃脾门静脉高压症外科行脾极量切除术治疗的17例成人型戈谢病患者的临床与随访资料，术中失血量（953±584）ml，术后住院时间（14±3）d。与术前相比，术后患者生活质量评分提高，疾病严重程度评分降低。本研究表明，脾极量切除术应用于成人型戈谢病治疗经济、安全，可改善患者生存、生活质量，具有临床应用价值。

Objective To explore the feasibility and applicability of subtotal splenetomy for the treatment of adults Gaucher disease. Methods The clinical data of 6 patients with adult Gaucher disease who underwent subtotal splenectomy from October 2017 to February 2019 were retrospectively analyzed. All patients underwent splenectomy with preservation of the normal volume of the spleen innervated by the lower pole vessels of the spleen. The operational time, the intraoperative blood loss, the hospital stay, the postoperative complication of hemorrhage, pancreatic leakage and infection were noted. Haemocytes changes were observed. The volume of the spleen and the volume ratio of the original spleen were calculated. The blood flow status of the retained spleen was observed. The reticuloendothelial cell function of the retained spleen was evaluated. Results 6 cases of subtotal splenectomy were successfully completed with no conversion to total splenectomy during operation, no ischemic necrosis of retained spleen after subtotal splenectomy, no death during perioperative period. The average operation time was 98 min (80- 120 min), the average intraoperative blood loss was 320 ml (200- 500 ml), and the average postoperative hospital stay was 13 d (12- 15 d). No postoperative complications such as abdominal hemorrhage, pancreatic leakage and infection occurred. Postoperative white blood cell count returned to normal or slightly higher than the normal value, and platelet count rapidly increased, which was higher than the normal value or doubled, and red blood cell count and hemoglobin value slowly increased, which gradually appoached the normal value. Color Doppler ultrasound and enhanced CT showed that the preserved splenic artery branch blood flow was smooth, the distribution of blood supply to the spleen was even without ischemia and infarction. Although there was partial thrombosis in the splenic vein trunk, the blood flow was smooth. The average volume of retained spleen was 312 ml (265- 360 ml), accounting for 6. 3％ (5％ - 10％ ) of total volume, 99mTc isotope imaging showed that the retained spleen tissue nuclide distribution was basically uniform, and reticuloendothelial cell phagocytosis was good. Conclusion Subtotal splenectomy is a safe, feasible, cost-effective alternative treatment for adult Gaucher disease.

目前胃癌的主要治疗方式仍是手术治疗,标准D2根治术已得到推广,但胃癌术后的局部复发仍是导致患者远期预后不佳的重要因素.早期胃癌患者的检出率低和手术淋巴结清扫的不规范及胃周软组织切除的不彻底是导致胃癌患者局部复发的重要因素.全直肠系膜切除(TME)和完整结肠系膜切除(CME)对降低结直肠癌术后局部复发效果明显,相同进展程度下远期预后明显好于胃癌.近年提出的完整胃系膜切除治疗胃癌可能会降低胃癌术后局部复发,改善患者预后,规范了完成胃癌根治术的完整流程标准,对于胃癌手术的规范化实施达到整块切除具有指导意义,随着微创理念不断的深入,腹腔镜的应用与发展使我们对系膜的认识更加深入,我们对完整胃系膜切除治疗胃癌的现状及研究进展进行综述如下.

Pancreatic cancer remains one of the most lethal malignancies worldwide.Most tumors are discovered late in the tumorigenic process with over 80％ of patients ineligible for surgical resection,resulting in very low rates (＜ 5％) of five-year survival of pancreatic cancer patients.In the recent years,long non-coding RNAs (lncRNAs) have drawn great attention since they regulate almost all the aspects of cell biological behaviors,and regulate the progression and metastases of pancreatic cancer.Therefore,the present article aims to review latest advances in the underlying molecular mechanisms of lncRNAs in pancreatic cancer.In this review,we have discussed the regulatory roles of lncRNAs in cell cycle progression,apoptosis,autophagy and epithelial-mesenchymal-transition and cancer stem cells.