<正>近日,山西省晋城市首批面向驻市部队的99套经济适用房全部建成并投入使用,领到经济适用房钥匙的部队官兵,兴高采烈地搬进新居。这是晋城军分区积极推进军民融合式发展结出的又一硕果。

<正>山西省晋城军分区党委坚持把学习作为用党创新理论武装头脑、全面提高官兵能力素质、忠实履行使命任务的第一需要,紧密结合单位实际,努力在强化学习机制、创新学习方法上下功夫,建立"每月一讲、每月一考、每人一档"的学习制度,提高了学习质

<正>盛夏时节,记者在山西省军区所属单位看到,遍布营院的标语、展板,营造了浓厚的安全氛围,"人防"与"技防"完美结合,对库、站、室、所等重点目标形成严密防控,各级机关办公秩序正规有序。面对记者惊诧的目光,省

<正>日前,山西省军区组织各军分区、人武部,预备役师、旅、团等单位200余名军事主官齐聚晋城军分区,在学习贯彻从严治军有关文件精神、聆听专家学者就贯彻新条令专题辅导、观看省军区加强正规化管理录像片的基础上,实地参观了晋城军分区机关、高平市人武部正规化管理情况,推广了晋城军分区"四位一体"部队管理新模式,这是该省军区学习贯彻落实新条令的又一实际举措。

<正>山西省晋城军分区最近举办了"崇尚学习强素质、爱军精武立新功"电视知识竞赛,这是该部党委机关在开展深化当代革命军人核心价值观主题教育活动中的配合活动之一。这次知识竞赛是贯彻党中央提出建设学习型党组织的战略决策和省军区党委开展"争创学习型党组织、争建学习型机关、争当学习型干部"活动的指示要求,

盛夏时节,记者在山西省军区所属单位看到,遍布营院的标语、展板,营造了浓厚的安全氛围,人防与技防完美结合,对库、站、室、所等重点目标形成严密防控,各级机关办公秩序正规有序。面对记者惊诧的目光,省

The completed NS1 gene was amplified from PPV DNA by PCR method.Then the products were cloned into pET32c vector and the sequence was determined.The recombinant plasmid pET32c-NS1was transformed into BL21 competent cells and expressed in super-high level by using low-temperature induction to reduce formation of inclusion body.Western-blot analysis proved the renaturation protein has a good immunoreactivity against PPV antibody.The indirect ELISA method for the detection of PPV specific antibody in procine serum was established,after the optional working circumstances for the ELISA assay(antigen concentration:2.5 mg/L;optimal serum dilution:1∶200) with chessboard titration.The recombinant NS1 can be applied in differential diagnosis of PPV infections.

The completed NS1 gene was amplified from PPV DNA by PCR method.Then the products were cloned into pET32c vector and the sequence was determined.The recombinant plasmid pET32c-NS1 was transformed into BL21 competent cells and expressed in very high level as inclusion body when induced with IPTG.Western blot analysis proved the renaturation protein with good immunoreactivity against PPV antibody.The indirect ELISA method for the detection of PPV specific antibody in procine serum was established with the optional working circumstances for the ELISA assay(antigen concentration : 3.1 μg/mL;optimal serum dilution :1∶100) using chessboard titration.The positive criterion of this ELISA method was ODtesting serum≥0.4 and ODtesting serum/ODstandard positive value≥2.1.

<正>猪细小病毒病是由猪细小病毒(Porcine Parvovirus.PPV)感染引起以母猪繁殖障碍为主要特征的病毒性传染病,其特征是母猪孕前期感染该病毒后,经胎盘使胚胎或胎儿受到侵袭,引起母猪

After the revival of PRRSV S-1 strain by Marc-145 cells,the ORF5 and ORF7 genes of PRRSV S-1 strain were amplified by RT-PCR and cloned directly into the prokaryotic expression vector pET-32.The sequence analysis showed that the variability of ORF5 gene was relatively high and the homology between the PRRSV S-1 and VR-2332 strains and between the PRRSV S-1 and CH-1a strains was respectively 91% and 89% in nucleotide and 87% and 85% in amino acid.The ORF7 gene was relatively conservative,and compared with the VR-2332 and Ch-1a strains the homology was respectively 97% and 94% in nucleotide and 95% and 93% in amino acid.

The prokaryotic expression vector for PRRSV GP5 protein and N protein had been constructed,named pET-E and pTE-N.These vectors were transfarmed to the E.coli(BL21) for expression under induction of IPTG.SDS-PAGE and western blotting analysis showed that the fusion proteins with 39 kDa and 31 kDa could react with pig serum containing antibody against PRRSV.The result provided fundamental data and materials for the further investigation on immunogenicity function of GP5 and N of PRRSV.

In this study , the open reading frame of PPV VP2 and NS1 were amplified by PCR method from the PPV infected ST cells. Then the products were cloned in prokaryotic vector and pET32c/VP2 and pET30a/NS1expressed were construed respectively .The plasmids were introduced into E. coli BL21. After induction by IPTG , the high expression were found in products of pET32c/VP2 and pET30a/NS1 . Biological activity of the recombinant protein were detected by Western-blot , which can be applied in differential diagnosis of PPV infections.