Tobacco (Nicotiana tabacum Linn.) is a famous traditional herb used in folk medicine. The essential oils of tobacco have been demonstrated in modern studies to possess antioxidant, anti-inflammatory, and neuroprotective properties, while its anxiolytic effect has not been reported. The purpose of this study was to evaluate the anxiolytic effect of Yunnan tobacco essential oil (YTO) and Zimbabwe tobacco essential oil (ZTO) on mice. The constituents of YTO and ZTO were analyzed by GC/MS. The anxiolytic effect of YTO and ZTO (0.1%, 1%, and 10%, v/v) on male ICR mice was evaluated in the light–dark box test (LDB) and the elevated plus maze test (EPM) test via inhalation and transdermal administration. After the behavioral tests, salivary corticosterone levels in mice were measured. The behavioral analysis showed that the administration of both YTO and ZTO elevated the time that the mice spent in the light chamber in the LDB test compared to the untreated control. In the EPM test, YTO and ZTO increased the time spent in open arms and the number of entries into the open arms. In addition, both YTO and ZTO significantly decreased salivary corticosterone levels in mice (p ≤ 0.001). In summary, our results demonstrated that inhalation and transdermal administration of both YTO and ZTO showed anxiolytic effect on male ICR mice.

[目的]探究烟碱与苯甲酸是否可结合形成稳定的复合物.[方法]通过Job's Plot方法、核磁共振氢谱(1H NMR)、红外光谱、等温滴定量热法(ITC)以及理论计算确定了两者间的相互作用方式.[结果](1)通过Job's Plot方法测定了烟碱与苯甲酸的结合比例为1:1.(2)通过核磁共振氢谱及红外光谱确定了烟碱与苯甲酸之间存在相互作用力,确定了两者的结合方式.(3)运用等温滴定量热法再次验证了烟碱与苯甲酸的结合比例,测定了两者结合过程中的热力学数据(ΔH=-26.8 KJ/mol;ΔG=-33.99 KJ/mol;ΔS=24.12 J/mol?K)及结合常数(Ka=9.021×105 M-1).(4)通过理论计算得出两者的结合能E=-18.64 Kcal/mol.[结论]烟碱与苯甲酸之间存在较强的结合力,可以结合形成1:1的复合物,结合过程是自发的放热过程,复合物拥有更高的热解温度.

采用水蒸气蒸馏法制备烟草挥发物,优化了工艺条件,采用GC-MS分析了烟草挥发物中的香味成分,将最优条件下制得的烟草挥发物应用于电子烟烟液的制备,通过感官评价分析其应用效果.结果表明:1)水中蒸馏方式烟草挥发物的得率明显高于水上蒸馏方式;2)最佳蒸馏条件为浸润时间2 h,蒸馏时间6 h,蒸馏体系中NaCl质量分数为5％;3)采用水中蒸馏方式,在最佳蒸馏条件下,烟草挥发物中致香成分主要包括新植二烯、茄酮、大马烯酮、巨豆三烯酮、香叶基丙酮、法尼基丙酮等,烟碱未检出;4)在最佳蒸馏条件下且同样稀释2000倍时,水中蒸馏获得的烟草挥发物香韵丰富,烟草本香突出,香气量充足,香气浓郁、厚实,优于水上蒸馏获得的烟草挥发物品质,更能满足电子烟所需传统卷烟的烟草风格和烟气状态.

通过Koenigs-Knorr糖苷化反应合成了柏木醇葡萄糖苷,对产物进行了表征,确认了化合物的结构,并对反应条件进行了优化,提高了收率.同时,对柏木醇葡萄糖苷的香气进行了评价,将其应用于卷烟中能明显改善烟气的状态及香气品质.

利用蜂蜜分别与酪氨酸、缬氨酸、天冬氨酸、天冬酰胺酸、谷氨酸5种氨基酸制备了美拉德香料,并进行了烟用感官评价.其中缬氨酸的蜂蜜美拉德香料整体效果最佳,进一步对其主要致香成分进行了剖析,其中吡嗪类、麦芽酚、糠醛类对整体的焦甜香香韵起到了关键性的作用.

通过两步反应合成了新型凉味剂4-MPF,并对催化剂的选择进行了考察,对物料配比进行了优化,对分离方法进行了改进.进一步对凉味剂4-MPF在卷烟中的应用进行了评价,在卷烟中能显著改善香气质和香气量,与烟香协调性较好,并且能明显增加烟气的清凉感.

Cyclopropylcoumarin was synthesized via one-step Corey-Chaykovsky reaction using coumarin as raw material. The structure of the final compound obtained was confirmed by 1H NMR、13C NMR、HRMS spectra. Several reaction parameters were optimized to enhance the final yield. Furthermore, its application effects in tobacco were studied and the results proved that it can improve the smoking and flavor quality.

The aroma fingerprints of three different flavor tobaccos based on volatile/semi-volatile components have been analyzed by using closed-loop stripping analysis. 20 kinds of volatile/semi-volatile compounds were identified and quantified according to the content, aroma characteristic and repeatability. Differences in content of volatile/semi-volatile compounds were distinguished by discriminant analysis: rich tobaccos generally had higher contents of aroma compounds except neophytadiene. Southeast had a great difference with Huang-Huai areas; Moderate tobaccos showed no significant differences, North areas had a good identification; Freshly tobaccos had a higher content of neophytadiene which considered as the main cause of freshly flavor, Fujian showed a more obvious aroma characteristic.