Background: Gliomas are the most frequent and dangerous primary cerebral tumors. Therefore, there is a need to develop molecular targets for the diagnosis and treatment for glioma. Methods: In September 2020, we retrieved the expression matrix of glioblastoma (GBM) sufferers and pertinent clinical data from the TCGA (The Cancer Genome Atlas) database. Prognostic differences between various families with sequence similarity 110 member C (FAM110C) expression groups were assessed by Kaplan-Meier with log-rank test. The R platform get used to assess the accuracy of FAM110C delivery in predicting the prognosis of PDAC using a time-dependent receptor operating characteristic (ROC) curve. The delivery level of FAM110C was determined by qRT-PCR and western blot. Gene set enrichment investigated possible mechanisms between different FAM110C expression groups in GBM (GSEA). The impact of FAM110C on glioma cell movement was discovered using migration test. The drug's gene-targeting impact was validated by the CCK8 test. Results: A total of 173 GBM samples were obtained from the TCGA database, with 148 including information on IDH1 mutations and 151 containing information on overall survival. The mRNA expression level of FAM110C was greater in wild-type GBM, according to qRT-PCR data. The connection between FAM110C expression and Hallmark, GO, and KEGG pathway gene sets was investigated using GSEA software. We used migration test to assess the impact of FAM110C on glioma motility in order to confirm the findings of the GSEA analysis. Conclusion: FAM110C might get used as a possible diagnostic and prognostic biomarker for wild-type GBM, and its inhibition could be used to prevention and treatment wild-type GBM.

目的 探讨齐墩果酸(oleanolic acid,OA)/5氟尿嘧啶(5-fluorouracil,5FU)协同对肝癌细胞内Ca2+表达水平的影响及其对JNK通路诱导细胞凋亡的作用机制,为临床治疗提供可靠的依据.方法 通过MTT和集落形成实验评估各用药组在肝癌细胞BEL-7402中的作用;采用Ca2+荧光探针检测肝癌细胞中Ca2+分布,并通过流式细胞术分析Ca2+表达水平;采用免疫荧光染色检测肝癌细胞中Ca2+通道相关蛋白Grp75的表达;通过Hoechst 33342染色检测肝癌细胞的凋亡情况;通过Western blot检测Ca2+通道相关蛋白和细胞凋亡相关蛋白的表达.结果 在JNK信号通路抑制剂SP600125作用下,与空白组比较,OA/5 FU协同显著降低了体外肝癌细胞的生存活力(P<0.05);克隆结果表明:与空白组比较,OA/5 FU协同可抑制肝癌细胞的集落形成能力(P<0.01);Ca2+荧光探针检测结果显示:与空白组比较,OA/5 FU协同可增加肝癌细胞内Ca2+的表达水平(P<0.01).免疫荧光结果显示:与空白组比较,OA/5 FU协同可降低Ca2+通道相关蛋白Grp75的表达.Hoechst 33342染色结果表明:与空白组比较,OA/5 FU协同可诱导肝癌细胞凋亡.Western blot结果显示:与空白组比较,OA/5 FU协同可调控Grp75、PDI、VDAC1、BCL-2、Bax、cl-Caspase3蛋白的表达,抑制肝癌细胞增殖并诱导细胞凋亡.结论 OA/5 FU协同可诱导肝癌细胞凋亡、抑制细胞增殖,其机制可能是通过调控JNK信号通路影响肝癌细胞内Ca2+的表达水平从而达到抗肿瘤作用.

Background This study aimed to investigate FAM84B expression in glioma tissues and explore the role of FAM84B in promoting the proliferation of glioma cells and the mechanism of regulating the cell cycle pathways. Methods The TCGA database was adopted to analyze FAM84B expression in glioma tissues. The FAM84B expression was detected by qRT-PCR in patients with glioma, especially that in glioma cells, U251, LN-229, U98, and U87. Two glioma cell lines U87 and T98 were selected for siRNA transfection, which were divided into si-NC si-FAM84B-1 and si-FAM84B-2 groups. The effect of FAM84B on the proliferation of glioma cells was detected with the MTT experiment and that on the glioma cell cycle was detected with the flow cytometry. The signaling pathways potentially regulated by FAM84B in glioma were analyzed through the bioinformatics analysis. The expression of proteins, Cyclin D1, CDK4, Cdk6, and p21, in the cell cycle-related pathways in cells of each group was detected by the Western blot. Results TCGA database results showed a significantly higher FAM84B expression in glioma tissues than that in paracancerous tissues. According to the detection of qRT-PCR, FAM84B expressed the highest in the glioma cell line U87 ( P < 0.05). Compared with the serum of healthy controls, FAM84B mRNA expression significantly increased in patients with gliomas. And compared with the si-NC group, the proliferation ability of U87 and T98 cells decreased and the cell cycle was blocked in the G0/G1 phase in both si-FAM84B transfection groups ( P < 0.05). According to the bioinformatics analysis, FAM84B regulated the cell cycle pathways in glioma. FAM84B siRNA inhibited the expression of key proteins, Cyclin D1, CDK2, CDK4, and Cdk6, of the cell cycle pathways in glioma cells and promoted the expression of P53 and P21 proteins. Conclusions In conclusion, FAM84B may inhibit the proliferation of glioma cells by regulating the cell cycle pathways.

目前高校对医学生的培养主要表现为注重传统知识教学,忽视科研素质及创新精神的培养.本文以"线粒体膜电位检测"实验为例,利用"翻转课堂"教学模式,结合"雨课堂"平台初步探究了细胞生物学实验教学改革策略,以期提高医学生的科学素养.

Objective:To investigate the role of DcR3 expression in apoptosis and proliferation of hepatocellular carcinoma cells.Methods:In this study，the Dcr3 lentivirus vector was constructed，and the non-specific vector was established.After transfection into HepG2 cell lines，the transfection efficiency was observed and recorded.Dcr3 protein expression level and apoptosis index transcriptional level were detected.Results:The expression levels of DcR3 protein in HepG2，MHCC-LM3 and MHCC-97H were detected.It could be seen that the level of HepG2 cell line was lower than that of other MHCC-LM3 and MHCC-97H(P<0.05).After upregulating the mRNA level of HepG2 cell line DcR3，the transcription levels of Caspase3 and Bax in apoptosis indicators were significantly decreased，while the transcription levels of bcl-2 in anti-apoptotic genes were increased(P<0.05).The proliferation rate of LV-DCR3 group was significantly higher than that of LV-NC group(P<0.05) when hepatoma cells were cultured for 4 and 6 days(P<0.05).Conclusion:The expression of DcR3 plays an obvious role in the apoptosis and proliferation of hepatocellular carcinoma cells，which can promote the proliferation and anti-apoptosis of hepatocellular carcinoma cells.The analysis of the reason is mainly related to the up-regulation of the expression of anticancer gene Bcl-2.

在以大数据、数字化、智能化、互联网等为主要特征的信息化时代背景下,如何提高高等医学院校的教学质量和教学水平已成为重要的教育课题.高等医学教育在理念、内容、模式和方法等方面的变革正在探索中.为了培养新时代背景下具有较高分析问题、解决问题和沟通协作能力的医学生,作为生命科学中的核心和技术学科的医学细胞生物学,其教学模式的改革就显得尤为必要.文章从学生需求出发,从理论教学和实践教学进行教学模式的改革,采用新技术为学生学习提供良好的学习环境和实践平台,完善多元化评价体系,实现师生有效的互动.因此,构建符合学校办学特色的,具有实践性、自主性、发展性、综合性和开放性,以学生为主体的有机结合的模块式教学就成为医学细胞生物学教学的重点研究内容.

为了培养新时代背景下具有较高分析问题和解决问题能力的医学生,医学细胞生物学教学模式的改革就显得尤为必要.从学生需求出发,从理论教学和实践教学两方面构建的以学生为中心的模块式教学成为了医学细胞生物学教学的重点研究内容.该模块式教学模式的应用,不仅优化了教学内容,完善了评价体系,而且也达到了预期的教学目标,体现了学生的主体地位,激发了学生的学习热情,培养和提高了学生的自主学习能力.

随着移动通讯技术和信息技术的发展和各种移动终端的普及,推动了学生学习方式和教师授课模式改革探索的步伐.除微课、慕课、翻转课堂教学模式之外,在线资源与传统教学有机结合的混合教学模式已经被大多数高校教师接受和认可.为了在有限的学习时间拓展医学生学习的深度和广度,学校《医学细胞生物》以超星网络教学平台为载体,实行线上线下混合式教学模式.该模式优于"教师讲授为主"的传统教学模式,不仅促进了课堂师生互动交流,提高了教学效率,而且可以促使学生自主学习.本文从教学准备、教学实施和教学效果评价等方面综合分析线上线下混合教学的优势,为不断建设、完善和优化多元化教学模块,提高教师教学质量、促进课程教学改革提供实践经验.

目的 探究蹄叶橐吾提取物(ethanolic extract of ligularia fischeri,EEOLF)的抗炎作用机制,为蹄叶橐吾(Ligularia fischeri,LF)的临床应用提供理论依据.方法 采用脂多糖(lipopolysaccharide,LPS)作用于RAW264.7细胞建立体外细胞炎症模型.实验分6组,即空白对照组、EEOLF组、LPS组、LPS+吡咯烷二硫代氨基甲酸盐(Ammonium pyrrolidinedithiocarbamate,PDTC)组、LPS+EEOLF组、LPS+EEOLF+PDTC组.Western blot法检测药物作用于RAW264.7细胞后,细胞核内因子κB p65(nuclear factor-κB,NF-κB)的表达水平和磷酸化水平.ELISA法测定药物作用于RAW264.7细胞后IL-1β及TNF-α的释放量.结果 与空白组对照比较,LPS能显著增加细胞核内NF-κB p65蛋白表达及其磷酸化水平(P＜0.001);与LPS组比较,EEOLF能显著下调LPS诱导增加的NF-κB p65蛋白表达及其磷酸化水平(P＜ 0.001).与空白组比较,LPS能显著增加炎症因子L-1β及TNF-α的分泌(P＜0.001);与LPS组比较,蹄叶橐吾提取物(ethanolic extract of ligularia fischeri,EEOLF)能显著降低LPS诱导增加的L-1β及TNF-α的分泌(P＜ 0.001).在NF-κB抑制剂PDTC作用下,LPS诱导的细胞炎症反应减弱,并且在PDTC存在下,EEOLF能更加明显降低LPS所诱导的细胞炎症反应.结论 EEOLF通过作用于NF-κB通路抑制炎性因子的分泌,从而达到抗炎作用.

近些年齐齐哈尔医学院补考和重修问题较为突出,统计数据表明重修2次及以上学生的不及格率较高.互联网技术的广泛应用,为这些学生专业知识的学习提供了新的途径和可能.可以将重修学生分类建群,由专门教师负责组织他们在网络教学平台学习.改革完善补考和重修制度,每门课程应限定补考和重修次数,总计不应超过3次;建议可将考试时间安排在开学后两周进行,学生可以得到老师和同学的辅导帮助,取得更好成绩.

将小组讨论与Mini-CEX教学方法融合运用于细胞生物学课程中,针对医学生缺乏主观能动性、被动填鸭式学习的问题,根据细胞生物学课程的基础性、实践性、实用性等特点,结合先进的教学评估理念进行全面改革.通过学习模式重构、教学投入的重构、教师角色重塑等路径广泛开展参与式教学,激发学生间的协作精神,提高教学效率.改革目的在于结合小组讨论与Mini-CEX评价模式,对教学模式进行全面反馈,通过基础知识、实践操作和沟通交流并重的教学方法,培养医学生的综合能力、团队合作精神以及人际交往能力,实现理论实践的提升,提高细胞生物学的教学质量.

Photodynamic therapy (PDT) is considered to be an advancing antitumor technology. PDT using hydrophilic/lipophilic tetra-alpha-(4-carboxyphenoxy) phthalocyanine zinc (T alpha PcZn-PDT) has exhibited antitumor activity in Bel-7402 hepatocellular cancer cells. However, the manner in which p38 MAPK and caspase-9 are involved in the regulation of mitochondria-mediated apoptosis in the T alpha PcZn-PDT-treated LoVo human colon carcinoma cells remains unclear. Therefore, in the present study, a siRNA targeting p38 MAPK (siRNA-p38 MAPK) and the caspase-9 specific inhibitor z-LEHD-fmk were used to examine the crosstalk between p38 MAPK and caspase-9 during mitochondria-mediated apoptosis in the T alpha PcZn-PDT-treated LoVo cells. The findings revealed that the T alpha PcZn-PDT treatment of LoVo cells resulted in the induction of apoptosis, the formation of p38 MAPK/caspase-9 complexes, the activation of p38 MAPK, caspase-9, caspase-3 and Bid, the downregulation of Bcl-2, the reduction of mitochondrial membrane potential (Delta Psi m), the upregulation of Bax and the release of apoptosis-inducing factor (AIF) and cytochrome c (Cyto c). By contrast, siRNA-p38 MAPK or z-LEHD-fmk both attenuated the effects of T alpha PcZn-PDT in the LoVo cells. Furthermore, the results revealed that siRNA-p38 MAPK had more significant inhibitory effects on apoptosis and mitochondria compared with the effects of z-LEHD-fmk in T alpha PcZn-PDT-treated LoVo cells. These findings indicated that p38 MAPK plays the major regulatory role in the crosstalk between p38 MAPK and caspase-9 and that direct interaction between p38 MAPK and caspase-9 may regulate mitochondria-mediated apoptosis in the T alpha PcZn-PDT-treated LoVo cells.

目的 探讨医学细胞生物学实验中引入微课教学及Mini-CEX评价式教学法的应用效果及可行性.方法 将齐齐哈尔医学院2017级临床医学专业的本科生分为对照组和实验组,对照组进行传统讲授式教学,实验组结合课程特点进行微课教学及Mini-CEX评价式教学,通过综合成绩和教学反馈问卷评估教学效果的优劣.结果 实验组成绩优于对照组(P<0.05),差异有统计学意义;实验组问卷结果也优于对照组,学生满意度高.结论"微课"结合Mini-CEX评价式教学在医学细胞生物学实验中具有良好的教学效果,使学生在操作能力、解决问题能力、沟通能力等方面有明显提高,实现"以考促学"有效提升学生的综合素养,值得进一步探索推广.

Our previous study indicated that anti-Fas antibody/actinomycin D (AF/AD) induced apoptosis of human hepatocellular carcinoma Bel-7402 cells; however, crosstalk influence between P38MAPK and autophagy on mitochondria-mediated apoptosis induced by AF/AD in Bel-7402 cells remains unclear. Therefore, effect of AF/AD on apoptosis, autophagy, phosphorylated-P38MAPK (p-P38MAPK), and membrane potential (ΔΨm) with or without the P38MAPK inhibitor SB203580 or the autophagy inhibitor 3-methyladenine (3-MA) in Bel-7402 cells was investigated in the present study. The results showed that AF/AD resulted in induction of apoptosis concomitant with autophagy, upregulation of p-P38MAPK and autophagy-associated gene proteins (Atg5-Atg12 protein complex, Atg7, Atg10, Beclin-1, LC3 I, and LC3 II), and downregulation of ΔΨm in Bel-7402 cells. In contrast, SB203580 attenuated the effects of AF/AD in Bel-7402 cells. Furthermore, the findings also demonstrated that 3-MA inhibited the impact of AF/AD on autophagy, Atg5-Atg12 protein complex, Atg7, Atg10, Beclin-1, LC3 I, LC3 II, and ΔΨm, and promoted the influence of AF/AD on apoptosis and p-P38MAPK in Bel-7402 cells. Taken together, we conclude that crosstalk between P38MAPK and autophagy regulates mitochondria-mediated apoptosis induced by AF/AD in Bel-7402 cells.

构建医学细胞生物学与遗传学教与学评价体系.对教师教学工作的评价包括督导评价、教研室评价、学生评价,教师自评;构建形成性考核、阶段测验(或期中考试)、终结性考核相结合的学生评价体系,学生互相评价作为有益补充.教与学评价体系的构建,使教学过程中教与学两个方面都得到了有效的监督和管理.

目的 探讨迷你临床演练评量(Mini-CEX)教学模式应用到细胞生物学实验教学中的可行性.方法 结合课程特点,采用Mini-CEX教学模式,以考促学,通过教学实践活动给予学生客观综合评价及反馈意见.并对开展Mini-CEX教学模式的实验组和传统教学模式的空白对照组分别进行问卷调查.结果 开展Mini-CEX教学模式的实验组综合成绩高于空白对照组,学生满意度高,结果 差异有统计学意义(P<0.05).结论 Mini-CEX评价模式应用于医学细胞生物学实验课教学具有可行性,可有效提高教学效果.

目的 探讨在FAS/AD抑制Bel-7402细胞增殖过程中自噬的作用.方法 通过MTT法检测3-甲基腺嘌呤(3-MA)对细胞增殖的影响,通过免疫荧光法检测LC3及Beclin 1的表达变化,通过Western印迹检测Atg12-5和Atg7的表达,以揭示FAS/AD对Bel-7402细胞的自噬作用.结果 与对照组相比,FAS/AD组的细胞的自噬现象明显,LC3及Beclin 1高表达,同时Atg12-5和Atg7也高表达,而FAS/AD/MA组的活力明显降低,自噬现象较弱,同时LC3、Beclin 1及Atg12-5和Atg7的表达降低.

将迷你临床演练评量(Mini-CEX)教学方法应用于《细胞生物学》的实验教学中,针对学生被动灌输的习惯及知识运用时的生搬硬套问题,根据课程特点进行教学方法改革,提高学生的自主学习性和参与度.迅速提高学生的操作能力,利用个性化反馈弥补知识框架中的薄弱点,培养学生的综合能力.并设计Mini-CEX评价表及调查问卷,总结反馈意见,实现以考促学、教学相长.现以教学互动理论为依据,旨在提高细胞生物学教学的质量.

Due to the critical role of glucose level in the diagnosis and treatment of diabetes, as well as the increasing number of diabetics, there is an overwhelming demand for developing glucose sensors. It is well acknowledged that these sensors, especially those based on glucose oxidase, have played an important role in blood glucose detection. Inspired by the attractive properties, nanomaterials, especially nanostructured carbon and metal/metal oxides, have been extensively explored to develop enzymatic glucose sensors with high sensitivity, fast response time, and satisfied stability. In this review, a brief history of glucose biosensors is firstly presented. Furthermore, we discuss the currently available fabrication possesses in the field of enzymatic glucose biosensors based on nanomaterials, focusing on the carbon-based, metal-based, and metal oxides-based nanocomposites. What is more, we discuss the challenges and attempt to give an outlook on the possible further developments.

探讨以遗传病例导向为主的讨论式教学方法在医学遗传学教学中的应用效果，为提高教师的教学质量以及学生成绩提供依据。通过案例设计、分组讨论、课堂交流等形式实施讨论式教学，完成授课后发放调查问卷评价讨论式教学的实施效果，分析探讨讨论式教学在医学遗传学中的应用情况，并通过合理设置满足所有学生的课堂需求。