Salt dietary intake is tightly coupled to human health, and excessive sodium can cause strokes and cardiovascular diseases. Research into the renal medulla of camels exhibiting high salt resistance may aid identification of the mechanisms governing resistance to high salinity. In this study, we used RNA sequencing (RNA-seq) to show that in the renal medulla of camels under salt stress, 22 mRNAs, 2 long noncoding RNAs (lncRNAs), and 31 microRNAs (miRNAs) exhibited differential expression compared with the free salt-intake diet group. Using fluorescence in situ hybridization and dual-luciferase reporter assays, we demonstrated that the lncRNA LNC003834 can bind miRNA-34a and thereby relieve suppression of the salt-absorption-inhibiting SLC14A1 mRNA from miRNA-34a, suggesting that the above lncRNA-miRNA-mRNA act as competing endogenous RNAs (ceRNAs). We subsequently performed short hairpin RNA and small RNA interference and reactive oxygen species (ROS) detection assays to show that SLC6A1, PCBP2, and PEX5L can improve the antioxidation capacity of renal medulla cells of camel by decreasing ROS levels. Our data suggest that camels achieve sodium homeostasis through regulating the expression of salt-reabsorption-related genes in the renal medulla, and this involves ceRNAs (SLC14A1 mRNA, LNC003834, and miRNA-34a) and antioxidant genes (SLC6A1, PCBP2, and PEX5L). These data may assist in the development of treatments for diseases induced by high salt diets.

The Mongolian horses have excellent endurance and stress resistance to adapt to the cold and harsh plateau conditions. Intraspecific genetic diversity is mainly embodied in various genetic advantages of different branches of the Mongolian horse. Since people pay progressive attention to the athletic performance of horse, we expect to guide the exercise-oriented breeding of horses through genomics research. We obtained the clean data of 630,535,376,400 bp through the entire genome second-generation sequencing for the whole blood of four Abaga horses and ten Wushen horses. Based on the data analysis of single nucleotide polymorphism, we severally detected that 479 and 943 positively selected genes, particularly exercise related, were mainly enriched on equine chromosome 4 in Abaga horses and Wushen horses, which implied that chromosome 4 may be associated with the evolution of the Mongolian horse and athletic performance. Four hundred and forty genes of positive selection were enriched in 12 exercise-related pathways and narrowed in 21 exercise-related genes in Abaga horse, which were distinguished from Wushen horse. So, we speculated that the Abaga horse may have oriented genes for the motorial mechanism and 21 exercise-related genes also provided a molecular genetic basis for exercise-directed breeding of the Mongolian horse.

Purpose:Herbal medicine is one of crucial symbols of Chinese national medicine. Investigation on molecular responses of different herbal strategies against viral myocarditis is immeasurably conducive to targeting drug development in the current international absence of miracle treatment.Methods:Literature retrieval platforms were applied in the collection of existing empirical evidences for viral myocarditis-related single-herbal strategies. SwissTargetPrediction, Metascape, and Discovery Studio coordinating with multidatabases investigated underlying target genes, interactive proteins, and docking molecules in turn.Results:Six single-herbal medicines consisting of Huangqi (Hedysarum Multijugum Maxim), Yuganzi (Phyllanthi Fructus), Kushen (Sophorae Flavescentis Radix), Jianghuang (Curcumaelongae Rhizoma), Chaihu (Radix Bupleuri), and Jixueteng (Spatholobus Suberectus Dunn) meet the requirement. There were 11 overlapped and 73 unique natural components detected in these herbs. SLC6A2, SLC6A4, NOS2, PPARA, PPARG, ACHE, CYP2C19, CYP51A1, and CHRM2 were equally targeted by six herbs and identified as viral myocarditis-associated symbols. MCODE algorithm exposed the hub role of SRC and EGFR in strategies without Jianghuang. Subsequently, we learned intermolecular interactions of herbal components and their targeting heart-tissue-specific CHRM2, FABP3, TNNC1, TNNI3, TNNT2, and SCN5A and cardiac-myocytes-specific IL6, MMP1, and PLAT coupled with viral myocarditis. Ten interactive characteristics such as π-alkyl and van der Waals were modeled in which ARG111, LYS253, ILE114, and VAL11 on cardiac troponin (TNNC1-TNNI3-TNNT2) and ARG208, ASN106, and ALA258 on MMP1 fulfilled potential communicating anchor with ellagic acid, 5α, 9α-dihydroxymatrine, and leachianone g via hydrogen bond and hydrophobic interaction, respectively.Conclusions:The comprehensive outcomes uncover differences and linkages between six herbs against viral myocarditis through component and target analysis, fostering development of drugs.

Mongolian sheep and Dorper sheep as keystone sheep breed present excellent adaptability to the surroundings, but the heat-responsive genes of two sheep breeds are rarely explored, especially non-coding RNA gene. The three Mongolian sheep and three Dorper sheep were treated under heat condition, and the livers of six sheep were sampled and arranged to sequence. Using bioinformatics analysis to investigate RNA-seq data, 335 differentially expressed protein-coding genes and 65 long non-coding RNA (lncRNA) genes were emerged. In the liver of Mongolian sheep, 5 protein-coding genes relevant to heat response were inspected, including COMMD10, ABCA9, NIPAL1, APOA4 and LIN7A while 8 genes containing NUP62, PLIN2, ACACB, NIPAL1, EXO1, LGALS3, TRPM1 and TRPV2 were screened in liver of Dorper sheep. The biological effects of the 12 protein-coding genes were involved in heat shock protein binding, lipid homeostasis and storage, fatty acid oxidation, magnesium/calcium ions import, anti-oxidation and DNA repair. Among above heat response-centric genes, ABCA9 was predicted as target gene of significantly differential XLOC_476131. The selected 12 protein-coding genes and 1 lncRNA genes were considered to provide an understanding of how sheep positively respond to heat stress, and further to provide molecular evidence on heat-tolerance.

Mongolian sheep survive well on the Mongolian plateau during tremendously cold winters, but their cold response mechanisms are not well understood. By comparing with Dorper sheep originating from South Africa, we expected to reveal the cold tolerance mechanisms of Mongolian sheep on the basis of transcriptome data, further providing molecular evidence for the targeted breeding of sheep. Based on the mRNA data of RNA-seq, we selected candidate genes among the differentially expressed genes; 7 from adipose tissue, 33 from skeletal muscle, and 10 from the liver. According to enriched KEGG pathways and previous research results, the effects of the above candidate genes mainly involved weakening the synthesis of long-chain fatty acids, raising the adaptive thermogenesis and browning adipogenesis in fat, reducing glucose 6-phosphate, stimulating citrulline generation and enhancing lipid metabolism in skeletal muscle, and postponing cell senescence in the liver. The findings also demonstrate the regulation mechanisms of cold tolerance in different tissues of Mongolian sheep.

德保矮马(Equus caballus)是我国广西的优秀马品种,在体高方面与我国其他品种马匹有显著差异,但对德保矮马体尺发育的调控机制知之甚少.本研究对蒙古马和德保矮马在幼年和成年时期的垂体和长骨骨骺进行组织样本采集和转录组测序,得到不同发育阶段差异表达的长链非编码RNAs(long non-coding RNA,lncRNAs)847个,其中512个上调表达、335个下调表达;得到不同品种间差异表达的lncRNAs 1633个,其中882个上调表达、751个下调表达;另外,德保矮马成年和幼年发育时期垂体组织中差异表达的lncRNAs为41个,而长骨组织中为5个;蒙古马成年和幼年发育时期垂体组织中差异表达的lncRNAs为41个,而长骨组织中为7个;在幼年时期蒙古马和德保矮马垂体组织中差异表达的lncRNAs为59个,而长骨组织中为34个;在成年时期蒙古马和德保矮马垂体组织中差异表达的lncRNAs为32个,而长骨组织中为35个.将这些差异表达lncRNAs所对应的靶基因进行KEGG和GO(Gene Ontology)分析,结果主要涉及神经系统和内分泌系统发育等方面.根据差异表达基因相关的基因功能富集、通路富集和前人研究结果,共筛选出20个生长发育相关蛋白的编码基因,其中差异表达的lncRNAs中有13个是通过反式作用分别靶向生长激素(growth hormone,GH)和胰岛素样生长因子1(insulin-like growth factor-1,IGF-1)基因.本研究结果可为德保矮马体尺发育相关机制研究及靶向矮小性状的家畜育种提供参考依据.

Camels as a sort of animal long living in desert have evolved stress-resistance characteristics to adapt to environment with high temperature and water shortage environment. However, the research of non-coding RNA (ncRNA)-mediated molecular regulation about how camel responds to arid condition in post-transcriptional regulation level is deficient. Under water-deprivation stress, by RNA-sequencing of camel renal medulla associated with regulating water metabolism, we detected significantly differential 575 alternative splicing events (ASEs) and 17 mRNAs, 26 miRNAs and 0 lncRNA. The down-regulated ACLY and LOC105061856, up-regulated PCBP2 and miR-195 potentially targeting LOC105061856 and PCBP2 mRNA were selected as candidate resistance-related genes. In quantitative experiment, the expression level of above four genes was consistent with RNA-seq data by qRT-PCR. The suppressive cell dehydration with down-regulated ACLY, inhibitive aerobic respiration with down-regulated LOC105061856 targeted by miR-195 and strong anti-oxidative capability with PCBP2 aimed by miR-195 may be regulatory modes of camel renal medulla adapting to water-deprivation condition.

Camels have evolved various resistance characteristics adaptive to their desert habitats. In the present study, we used high-throughput sequencing to investigate stress-induced alternative splicing events as well as different genes involved in resistance to water deprivation and salt absorption in the ileum and liver in Camelus bactrianus. Through association analyses of mRNA, miRNA and lncRNA, we sought to explicate how camels respond to high salt and water scarcity conditions. There were two modes by which genes driven by alternative splicing were enriched to molecular functions, invoking of which was potentially fixed by organ and stress types. With qRT-PCR detection, the differentially expressed MUC6, AQP5, LOC105076960, PKP4, CDH11, TENM1, SDS, LOC105061856, PLIN2 and UPP2 were screened as functionally important genes, along with miR-29b, miR-484, miR-362-5p, miR-96, miR-195, miR-128 and miR-148a. These genes contributed to cellular stress resistance, for instance by reducing water loss, inhibiting excessive import of sodium, improving protective barriers and sodium ion homeostasis, and maintaining uridine content. The underlying competing endogenous RNAs referred to LNC001664, let-7e and LOC105076960 mRNA in ileum, and LNC001438, LNC003417, LNC001770, miR-199c and TENM1 mRNA in liver. Besides competent interpretation to resistance, there may be inspirations for curing human diseases triggered by high-salt intake.

旨在从基因组水平对蒙古马运动性状涉及的相关基因进行分析.本研究利用全基因组测序技术,对蒙古马的血液进行采样和基因组测序,并且结合人、牛、小鼠等7种哺乳动物的基因组数据,对蒙古马的特有基因和正向选择基因进行筛选.分析数据得到已注释的蛋白编码基因19 475种,并筛选出了38种蒙古马特有基因和45种正向选择基因.根据GO富集、KEGG通路富集分析和前人研究,发现包括MAPK13、CCN2、FSCN3、BTG1、TF等17种正向选择的蛋白编码基因可能参与蒙古马的运动过程.这些运动相关正向选择基因的生物学功能涉及到肌动蛋白丝结构、心肌收缩的正调节、钙离子结合、调节细胞内pH、血管生成、胶原生物合成过程的正调节等.而7种miRNAs(包括eca-miR-302c、eca-miR-8974、eca-miR-9077等)则通过与mRNA的3'UTR区域作用分别潜在靶向调控运动相关的MAPK13、LOC100057755、EHD4、MICAL3、TMBIM1和F11R.以上涉及的运动相关正向选择的蛋白编码基因可以促进人们对于蒙古马运动性状的了解,并为靶向竞技型的家畜育种提供分子理论基础.

可变剪接使单个基因产生多个mRNA,从而增强蛋白质的多样性,这与生物的生长发育过程密切相关.在中国丰富的马(Equus caballus)品种资源中,德保矮马和蒙古马具有明显的体高差异.本研究利用与马体尺大小密切相关的垂体组织,经RNA-seq和生物信息学分析,检测出可变剪接事件137 449个,主要以内含子保留(intron retention,IntronR)、可变的3'剪切位点(alternative 3'splice site,A3SS)、可变的5'剪切位点(alternative 5'splice site,A5SS)和外显子跳跃(exon skipping,ES)四种可变剪接类型为主.不同品种、不同生长时期马垂体的差异可变剪接基因共37种,其中,幼年德保矮马与幼年蒙古马的差异可变剪接基因23种,成年德保矮马与成年蒙古马的差异可变剪接基因1种,幼年德保矮马与成年德保矮马的差异可变剪接基因5种,幼年蒙古马与成年蒙古马的差异可变剪接基因8种.而幼年德保矮马垂体中WDR35(WD repeat-containing protein 35 gene)基因的外显子盒(cassette exon)可变剪接和OSBPL6 (oxysterol binding protein like 6)基因的ES可变剪接则被鉴别为影响德保矮马身高矮小的重要因素.此外,在德保矮马和蒙古马幼年至成年的发育阶段的垂体中,CPEB1、TCF12、CCDC28B、KIT、FAM129A、PTPRF、EPHA5、OGDH、USP28、EMID1、ANKHD1、KIDINS220和INF2共13种发生差异可变剪接的基因也被筛选作为马矮小性状的候选基因.上述矮小相关基因为进一步靶向马体高的分子育种提供了基础资料.

在马(Equus caballus)的繁殖和非繁殖季节,本研究探讨马扩展型(Ex)和紧凑型(Cp)卵丘-卵母细胞复合体(COCs)卵母细胞的孤雌激活效率.在繁殖季节,探讨马驹和成年马成纤维细胞核移植(SCNT)的成功率.孤雌激活实验结果显示,在繁殖季节,发育到2-细胞、4-细胞和桑椹胚的比例,扩展型(Ex)卵丘-卵母细胞复合体分别是52.8％ (19/36)、38.9％ (14/36)和5.6％ (2/36),紧凑型(Cp)卵丘-卵母细胞复合体分别是47.9％ (23/48)、33.3％ (16/48)和6.2％ (3/48).在非繁殖季节,发育到2-细胞、4-细胞的比例,扩展型(Ex)分别是37.2％ (16/43)和16.3％ (7/43),紧凑型(Cp)的比例分别是35.1％ (27/77)和11.7％ (9/77),都没有获得桑椹胚.同一季节,扩展型(Ex)与紧凑型(Cp)胚胎发育的比率差异不显著(P＞0.05),不同季节,两者差异显著(P＜0.05).体细胞核移植实验结果显示,以马驹成纤维细胞作为核供体细胞,胚胎发育到2-细胞、4～8细胞和桑椹胚的比例分别是41.5％(22/53)、33.9％ (18/53)和15.1％ (8/53),以成年马成纤维细胞作为核供体细胞,比例分别是38.9％(7/18)、22.2％ (4/18),没有获得桑椹胚.综上所述,季节和卵丘巧卵母细胞复合体(COCs)类型影响马卵母细胞孤雌激活的效率,不同核供体细胞影响克隆胚胎构建的成功率.

The Mongolian horses, as a neglected scientific resource, have excellent endurance and stress resistance to adapt to the cold and harsh plateau conditions. Intraspecific genetic diversity is mainly embodied in various genetic advantages of different branches of Mongolian horse. Abaga horse is better than Wushen horse in running speed, for example. Because people pay progressively attention to the athletic performance of horse, such as horse racing in Mongolia’s Naadam festival, we expect to guide the exercise-oriented breeding of horses through genomics research. We obtained the clean data of 630,535,376,400 bp through the entire genome second-generation sequencing for the whole blood of 4 Abaga horses and 10 Wushen horses. Based on the data analysis of single nucleotide polymorphism (SNP), we severally detected that 479 and 943 positively selected genes, particularly exercise-related, were mainly enriched on equine chromosome 4 in Abaga horses and Wushen horses, which implied that the chromosome 4 may be associated with the evolution of the Mongolian horse and athletic performance. Four hundred and forty genes of positive selection were enriched in 12 exercise-related pathways and narrowed in 21 exercise-related genes in Abaga horse, which were distinguished from Wushen horse. So, we speculated that the Abaga horse may have oriented genes for the motorial mechanism and 21 exercise-related genes also provided molecular genetic basis for exercise-directed breeding of Mongolian horse.

Objective: This study aims to discuss the role of remote ischemic preconditioning (RIPC) in gastric ischemia-reperfusion injury (GI-RI) through regulating nuclear factor kappaB (NF-kappa B). Method: A total of 114 healthy male SD rats were randomly divided into sham group (n = 6), I/R group (n = 36), RIPC group (n = 36) and NF-kappa B activation + RIPC group (n = 36). I/R, RIPC group, NF-kappa B activation + RIPC group were assigned into 6 sub-groups including reperfusion for 0 hour (T0), 1 hour (T1), 3 hours (T3), 6 hours (T6), 12 hours (T12) and 24 hours (T24). After 6 hours of reperfusion, the histopathological changes in gastric mucosal tissues were recorded and cell apoptosis was detected. At different time points after reperfusion, the gastric mucosal damage index (GMDI), malondialdehyde (MDA) level and superoxide dismutase (SOD) activity was determined. NF-kappa B p65 protein expression was detected using immunohistochemistry and Western blotting analysis. Results: The RIPC group showed slighter gastric mucosal damage than the I/R group. There were significantly higher GMDIs and MDA levels, decreased SOD activity and a higher NF-kappa B p65 protein expression in the I/R and NF-kappa B activation + RIPC groups compared to the sham group. The RIPC group displayed decreased cell apoptosis and MDA levels and increased SOD activity and NF-kappa B p65 protein expression in comparison to the I/R and NF-kappa B activation + RIPC groups. At T0, T1, T3 and T6, the RIPC group showed significantly lower GMDIs than the I/R and NF-kappa B activation + RIPC groups. Conclusion: RIPC may protect against GI-RI by inhibiting NF-kappa B activation and decreasing oxygen free radicals and neutrophil infiltration.

The aim of this study was to find out signaling pathways and candidate genes that related to cold tolerance of Mongolia cattle by RNA-sequencing and bioinformatics analysis of Mongolia cattle skin tissues in winter and summer.Transcriptome sequencing skin tissues of adult Mongolia cattle in winter and summer were carried out by Ion ProtonTM Sequencer,respectively.And then data processing,reference genome alignment,gene differential expression analysis,GO and KEGG enrichment analysis and candidate gene screening were applied on the transcriptome data;Additionally,the reliability of the transcriptome data was detected by Real-time PCR.The results showed that 86 954 060 and 69 837 009 reads were obtained from winter and summer groups,respectively;The percentage of the uniquely mapped reads was 73.21％ in winter group,and 75.55％ in summer group.There were 182 DEGs between winter and summer groups,compared to summer group,117 were up-regulated and 65 were down-regulated in winter group;Real-time PCR analysis showed that the transcriptome sequencing results were reliable;The results of GO analysis showed there were 21,39 and 224 terms enriched in the cellular components,molecular functions and biological processes,respectively;KEGG analysis showed that the DEGs were significantly enriched in lipid metabolism,blood coagulation,tyrosine metabolism,steroid hormone biosynthesis and retinol metabolism related pathways;8 candidate genes were screened out.In summary,lipid metabolism,blood coagulation and melanin synthesis related pathways and genes as well as hair synthesis related genes may play an important role in cold resistance of Mongolian cattle.

In order to find out signaling pathways that related to the differences in temperature adaptability between buffaloes and Yellow cattle, RNA-sequencing and bioinformatics analysis were performed on the skin tissues of 3 adult buffaloes and 3 adult Yellow cattle.The results showed that 59 369 167 and 69 837 009 reads were obtained from buffaloes and Yellow cattle skin, respectively.The percentages of the uniquely mapped reads in buffaloes and Yellow cattle were 70.76% and 75.55%, respectively.There were 1 611 differentially expressed genes in buffaloes and Yellow cattle skin, 801 of which were up-regulated in buffaloes skin and 810 were down-regulated in buffaloes skin.GO analysis showed that there were 316, 263 and 278 differentially expressed genes classified to the cellular components, molecular functions and biological processes, respectively.KEGG analysis showed that the differentially expressed genes were enriched in 33 signaling pathways, some of which such as neuroactive ligand-receptor interaction, oxidative phosphorylation, cholinergic synapse, serotonergic synapse, GABAergic synapse and calcium signaling pathway might related to the differences in temperature adaptability between buffaloes and Yellow cattle.In summary, the synapse and oxidative phosphorylation might be related to the differences in temperature adaptability between buffalo and Yellow cattle.This study would lay a foundation for further understanding the molecular mechanism of temperature adaptation differences between buffaloes and Yellow cattle and molecular breeding.

Current research has determined that many cloned animals have heterogeneous DNA methylation profiles. However, few studies have compared the methylation profiles of both naturally produced lambs and cloned lambs created using somatic cell nuclear transfer. The paucity of research in this area is because of insufficient resources to study limited cloned offspring, the ovine genome, and ovine genomic imprinting. In this study, to show the degree of reprogramming in cloned lambs, we cloned the putative differentially methylated regions (DMRs) of Peg3 from sheep and analyzed the DNA methylation patterns in CpG islands and DMRs of the putative imprinted genes Peg3, Cdkrzlc and GO in cloned lambs. We have provided evidence that Peg3 was highly methylated. The degree of methylation was 95.45% in the kidney and 88.18% in the lung of a natural sheep and 98.18% in the kidney and 87.27% in the lung for one cloned sheep. The bisulphite sequencing results for Cdknlc show complete non-methylation (0%, 0.53%, 0.53%, 0.53%) in all samples. In addition, Gd2 was hypomethylated in all lambs, from a linear correlation analysis, there were some differences in the quantitative values from both groups (correlation 72. 0.77). These data show that the DNA methylation status of the three imprinted genes was similar in cloned and natural lambs.

以呼和浩特市近郊大棚土壤为对象,采用常规理化分析、微生物纯培养方法分析了大棚土壤真菌种类、数量在12个月份、不同土层(0～5 cm、5～ 10 cm、10 ～ 15 cm、15 ～20 cm)的变化特征.结果表明:经纯化培养鉴定出8个属,镰孢属(Fusarium Link)、青霉属(Penicillium Lk.ex Fries)、腐霉属(Pythiumpringsh)、曲霉(Aspergillus)、红曲霉属(Monascus purpureus Went.)、金孢霉属(Chrysosporium)、毛霉属(Mucor)、木霉属(Trichoderma spp.).大棚真菌数量在12个月份、土层深度表现相应的变化.土壤真菌种类、数量在12个月份变化一致,即1～5月份真菌种类、数量均增加,6月达到峰值,7～12月份则依次下降.随土层深度的递增,土壤真菌种类变化呈现一定的减少趋势,其数量也逐渐降低.

The acetone extracts of parsley root materials obtained by four times column chromatography were used for exploring the effects of allelochemicals in the parsley root materials on physiological and biochemical aspects of Fusarium oxysporum f. sp. cucumebrium. Best column fractions were screened by the allelopathic inhibition effect and the activities of three cell wall degrading enzymes and fusaric acid content after 144 h of co-culture with F. oxysporum f. sp. cucumebrium in the liquid medium. The results indicated that the best column fractions obtained from the acetone extracts of fresh parsley root and rhizosphere soil inhibited the growth of F. oxysporum f. sp. cucumebrium. In comparison to CK,the cell wall degrading enzymes activities and fusaric acid contents decreased significantly with increased numbers of chromatography. The correlation analysis indicated that allelopathic inhibition effect had significant negative correlations with the activities of pectinase and β-glycosidase enzymes,but no significant correlation with the fusaric acid content.

本试验旨在研究绵羊孕酮控制释放器(CIDR)放置时间、不同饲养管理模式、重复超数排卵次数及超数排卵后发情时间对绵羊超数排卵的影响.CIDR放置不同时间结果显示,第10天起针组的平均可用胚胎数(P＜0.05)、胚胎可用率(P＜0.01)均高于第13天起针组;不同饲养管理模式结果显示,舍饲羊平均可用胚胎数、胚胎可用率极显著高于放牧羊(P＜0.01);不同次数超数排卵结果显示,第2次超数排卵效果好于其他5次,与第3次之间差异不显著(P＞0.05),与其他4次之间差异显著(P＜0.05);通过比较绵羊超数排卵后发情效果发现,绵羊超数排卵后发情24 h的胚胎利用效果最佳.综上所述,CIDR放置时间、不同饲养管理模式、重复超数排卵次数对供体绵羊超数排卵效果的影响极显著,且供体绵羊超数排卵后发情24 h的胚胎利用效果最佳.

It is necessary to treat a large numbers of synchronization of oestrus to recipients before the cow embryo transplantation. One of these study investigated the effects of synchronization of oestrus from two different ways, which including PG method and(CIDR+PG) method; the second test investigated the effects of synchronization of oestrus from parity(heifers, weaned cows and lactating cows) by PG method; the finally experiment surveyed the effects of synchronization of oestrus from different nutrition levels by CIDR + PG method. The consequence displayed that two processes did not remarkably affect the results of synchronization of oestrus(P>0.05). The comparison of the estrus synchronization rate of heifers was higher than others cows by PG method, and it showed that we can choose heifers as the best receptors.The trial investigated the optimal time of recipient cattle with calves is least 3 months under different nutrition levels of nature grazing conditions. There were kinds of results of parity and different nutrition levels and the optimal time of recipient cattle with calves' effect to synchronization of oestrus.