This study aimed to discover key genes associated with the in-vitro-fertilization embryo transfer (IVF‑ET) outcomes and develop new prediction models, as well as potential drugs. We enrolled the following infertile women who received IVF-ET between Sep 8, 2018, and Oct 19, 2021. The endometrial biopsy was performed for RNA sequencing. The differentially expressed genes (DEGs) were identified, based on which two models were developed for prediction of clinical pregnancy and live birth. Besides, we screened key pregnancy-lower and pregnancy-higher genes and identified candidate drugs that modulate the expression of key genes. Overall, 147 patients received IVF-ET, including 92 patients in the pregnancy group and 55 in the no-pregnancy group. We initially identified 180 differentially expressed genes (DEGs) between pregnancy and no-pregnancy groups, including 131 pregnancy-lower and 49 pregnancy-higher genes. After covariate-adjusted sensitivity analyses (age, RIF status, right-side AFC, and cycle type), 168 of these DEGs (93.3

Human extended pluripotent stem cells (hEPS) had been reported to be derived from discarded blastocysts, whereas the derivation method of hEPS cells was extremely complex in this protocol with feeder and animal serum conditions, which also limited the safety and homogeneity of hEPS. Here, we report an optimized, highly efficient protocol by utilizing chemically defined and xeno-free media for the derivation of human extended pluripotent stem cell lines from discarded blastocysts. With this method, we successfully isolated hEPS cell lines from discarded blastocysts with an efficiency of 46%. Chemically defined and xeno-free media simplified the process of hEPS cell isolation with a higher survival rate of cell aggregation passaging from outgrowth. To our knowledge, this is the first report of hEPS cells being efficiently derived from discarded blastocysts under chemically defined and xeno-free conditions.

There is a conspicuous lack of knowledge, attitudes, and practices (KAP) studies directed towards understanding the impact of diet on pregnancy among infertile individuals. Dietary habits can have a certain impact on infertility. To investigates the KAP of infertile individuals regarding the influence of diet on pregnancy. A cross-sectional study was conducted from July 2023 to January 2024 in Beijing, China. A structured questionnaire based on comprehensive literature review and validated by expert consultation was administered to gather demographic information, as well as to assess KAP among individuals experiencing infertility. The Cronbach’s α coefficient was 0.897, indicating high internal consistency. Statistical analyses included descriptive statistics, correlation analysis, logistic regression, and structural equation modeling. A total of 580 participants were included in this study. Of these, 494 individuals (85.17

Evaluating the quality of embryos and implantation potential is a critical determinant of in vitro fertilization and embryo transfer, and it is also one of the main challenges of assisted reproductive technology. A reliable non-invasive method to choose the best candidate with real implantation potential for transfer from two day-3 embryos with equal morphological quality is still lacking clinically. In this article, a sensitive LC-MS method was developed and metabolomic profiling analysis in a 3-day embryo culture medium was performed. Differential metabolites were analysed in two kinds of commercial culture media, and a total of 66 common metabolites were obtained from 106 independent samples in 5 batches. The relationship between changes in key metabolite, benzoic acid, concentration and the embryo implantation result was discovered. This work improved coverage through conditional optimization, enhanced the reliability of omics data through multi-batch validation, and provided a potential biomarker for evaluating the implantation potential of day-3 embryos.

Objective: To explore the association between snoring and in vitro fertilization (IVF) outcomes in infertile women, focusing on embryological parameters and pregnancy outcomes. Methods: This study represents a secondary analysis of the PKU-ERC study (NCT05373290). We included a cohort of 632 infertile women, aged 24-45 years, undergoing their first IVF treatment from the Reproductive Center of Peking University People's Hospital between January 2018 and November 2021. All patients with the assistance of their husbands completed a questionnaire including snoring status and frequency before ovulation induction (OI). Embryology parameters were evaluated during the first IVF cycle, and pregnancy outcomes were assessed through follow-up. Results: Among 579 subjects, 33.5 % reported occasional snoring, and 8.8 % reported frequent snoring. After adjusting for confounding factors, multiple linear regression model showed that frequent snorers had higher beta-coefficients for the number of blastocysts and available embryos compared to non-snorers (both P < 0.05). Among 551 subjects who completed the first embryo transfer, 6.2 % suffered biochemical pregnancy loss. Frequent snorers were more likely to experience biochemical pregnancy loss compared to non-snorers and occasional snorers (5.7 % vs. 14.6 %, P = 0.033; 4.8 % vs. 14.6 %, P = 0.026). Multivariable analysis revealed that frequent snoring was a risk factor for biochemical pregnancy loss (adjusted odds ratio, aOR: 2.95, 95 % confidence interval, CI: 1.06-8.24, P = 0.039), while high-density lipoprotein cholesterol (HDL-C) level was a protective factor after IVF (aOR: 0.21, 95 % CI: 0.05-0.92, P = 0.038). Conclusion: Frequent snoring is associated with a decreased number of available oocytes and an increased risk of biochemical pregnancy loss following IVF. However, the potential influence of undiagnosed obstructive sleep apnea (OSA) should be considered when interpreting these results.

Introduction: Epidemiological evidence over the last few decades has consistently shown that exposure to endocrine-disrupting chemicals (EDCs) is associated with adverse reproductive health outcomes, including male and female infertility, poor-pregnancy outcomes, and increased risk of diseases in childhood and beyond. To investigate the effects of EDCs and lifestyle on all aspects of reproduction (including early oocyte development, fertilization, embryo development, embryo implantation, abortion, and preterm birth).Methods: We performed this cohort study on patients receiving in vitro fertilization (IVF) treatment. Biological samples including urine, serum, follicular fluid, semen, fetal tissue, decidua, and placenta, were obtained.Results: By studying the correlations between reproductive outcomes and environmental pollutant exposure and lifestyle, we determined the toxicological mechanisms and health effects of EDCs on female reproductive health. We found that higher concentrations of per- and polyfluoroalkyl substances were correlated with polycystic ovary syndrome (PCOS). Using specific biomarkers, we also detected the concentrations of organophosphorus flame retardants (OPFRs) in urine and found that OPFRs may disrupt hormone homeostasis.Discussion: All of these results reveal EDCs may disrupt female reproduction.

BACKGROUND:The success of assisted pregnancy relies heavily on the effectiveness of the embryo transfer process. Currently, embryo transfer is typically conducted with the assistance of abdominal ultrasound.OBJECTIVE:The primary aim of this study was to evaluate the influence of targeted nursing interventions on the embryo transfer procedure, its impact on pregnancy outcomes, and the level of patient comfort concerning bladder management throughout the procedure.METHODS:A total of 247 patients who underwent embryo transfer at the Reproductive Center of Peking University People's Hospital from December 2019 to August 2020 were included in this study. These patients were categorized into two groups: the control group (n= 124) and the experimental group (n= 123). Within the control group, patients received conventional preoperative education, whereas those within the experimental group were subjected to targeted nursing interventions. Furthermore, patients in the experimental group were furnished with explicit instructions pertaining to the volume and timing of water intake. Multiple factors were assessed in this study, encompassing bladder filling, the quality of uterine imaging, the utilization of assistive devices during the surgical procedure, and pregnancy outcomes. Additionally, a post-operative questionnaire was administered to both groups to gauge their comfort levels regarding urinary retention.RESULTS:Following the targeted nursing intervention, ultrasound scans indicated an increase in bladder depth (5.91 ± 1.76 vs. 5.40 ± 1.61, P= 0.02), resulting in clearer endometrial imaging (96.74% vs. 88.71%, P= 0.02). Additionally, the experimental group reported significantly higher levels of comfort with urine retention (P= 0.01) compared to the control group, and these differences held statistical significance. Furthermore, the pregnancy rate in the experimental group was greater than that in the control group (52.85% vs. 50.8%, P> 0.05).CONCLUSION:Based on the premise that pregnancy rates remain unaffected, the implementation of targeted nursing care has the potential to augment bladder filling, enhance the quality of endometrial imaging, reduce the requirement for instrument-assisted embryo transfers, and notably enhance the comfort of patients in relation to urine retention.

Novel organophosphate flame retardants (OPFRs) are recently identified and highly detected in indoor dusts, but their personal exposure was not clear. Here, wristband was used to estimate non-dietary exposure to emerging OPFRs in comparison with legacy OPFRs in 93 adults in Beijing, China. Comparison of studies in wristband monitoring data showed a clear difference in profiles of legacy OPFRs between China and United States, where tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) was usually the dominant OPFR in the United States, but triphenyl phosphate has the highest contribution to total OPFRs in China. Five emerging OPFRs, including diethylene glycol bis(bis(2-chloroisopropyl) phosphate) (DEGBBCPP) and bis(2-ethylhexyl) phenyl phosphate (BEHPP), were detected in above 45 % of wristbands. The median concentration of DEGBBCPP (2.2 ng/g) was about three times higher than TDCIPP (0.76 ng/g), a legacy chloro-OPFR. Both emerging and legacy OPFRs were significantly correlated within 40 pairs of couples, suggesting major exposure in their homes. Wristbands from husbands had significantly higher tris(2-butoxyethyl) phosphate (TBOEP) and DEGBBCPP, while 2-ethylhexyl diphenyl phosphate (EHDPP) was significantly higher in wives' bands, suggesting gender-related exposure sources for these OPFRs.

Objective To analyze the effects of aryl organophosphate esters(aryl-OPEs)on female reproductive health.Methods Urine samples from 571 women with infertility who sought in vitro fertilization-embryo transfer treatment at the Reproductive Center of Peking University People's Hospital from January 2018 to January 2021 were tested for concentrations of four aryl-OPE metabolites:diphenyl phosphate(DPHP),4-hydroxyphenyl diphenyl phosphate(4-OH-TPHP),2-ethyl-5-hydroxyhexyl diphenyl phosphate(5-OH-EHDPP),and 4-(hydroxymethyl)phenyl di-p-tolyl phosphate(4-OH-MDTP).Continuous independent variable data were converted into categorical variables,divided into four levels(Q1,Q2,Q3,Q4)using the 25th,50th,and 75th percentiles as cut-off points.After reassigning values to the independent variables in each group,they were incorporated into the regression model as continuous variables for trend testing.Using multivariable linear mixed models,the correlations between aryl-OPE metabolite concentrations and reproductive hormones[estradiol(E2),progesterone(P),testosterone(T),follicle-stimulating hormone(FSH),luteinizing hormone(LH),and prolactin(PRL)]and infertility causes were assessed by adjusting for relevant covariates(age,BMI,smoking status,education level,infertility reason,and primary infertility diagnosis).Results The detection rates of the four aryl-OPE metabolites were all＞80％.The results of the multivariable linear regression model showed that the concentrations of 4-OH-MDTP and 5-OH-EHDPP in urine were associated with decreased E2 levels(P＜0.05).The E2 level in the 4-OH-MDTP Q3 group was significantly lower by 20.59％compared to the Q1 group,and in the 5-OH-EHDPP Q4 group,it decreased by 34.52％compared to the Q1 group.The exposure concentrations of 4-OH-TPHP and DPHP showed a significant positive correlation with progesterone levels(P＜0.05).The progesterone level in the 4-OH-TPHP Q3 group was significantly increased by 42.42％compared to the Q1 group,and in the DPHP Q4 group,it was significantly increased by 32.85％compared to the Q1 group.In the analysis of infertility causes,the concentrations of 4-OH-TPHP and 4-OH-MDTP in patients with polycystic ovary syndrome(PCOS)and endometriosis were significantly higher than those in women undergoing in vitro fertilization due to male factors.Moreover,the risk of PCOS and endometriosis increased in the 4-OH-TPHP and 4-OH-MDTP Q2-Q4 groups compared to the Q1 group.Conclusion Aryl-OPEs can interfere with estrogen and progesterone levels in women.The parent compounds and metabolites may be associated with the occurrence of PCOS and endometriosis and could be one of the causes of infertility.

We aimed to develop an accurate model to predict live birth for patients receiving in vitro fertilization and embryo transfer (IVF-ET) treatment. This is a prospective nested case–control study. Women aged between 18 and 38 years, whose body mass index (BMI) were between the range of 18.5–24 kg/m2, who had an endometrium of ≥ 8 mm at the thickest were enrolled from 2018/9 to 2020/8. All patients received IVF-ET treatment and were followed up until Jan. 2022 when they had reproductive outcomes. Endometrial samples during the window of implantation (LH + 6 to 9 days) were subjected to analyze specific endometrial receptivity genes’ expression using real-time PCR (RT-PCR). Patients were divided into live birth group and non-live birth group based on IVF-ET outcomes. Clinical signatures relevant to live birth were collected, analyzed, and used to establish a predictive model for live birth by univariate analysis (clinical model). Specific endometrial receptivity genes’ expression was analyzed, selected, and used to construct a predictive model for live birth by The Least Absolute Shrinkage and Selection Operator (LASSO) analysis (gene model). Finally, significant clinical factors and genes were used to construct a combined model for predicting live birth using multivariate logistical regression (combined model). Different models’ Area Under Curve (AUC) were compared to identify the most predictive model. Thirty-nine patients were enrolled in the study, twenty-four patients had live births, fifteen did not. In univariate analysis, the odds of live birth for women with ovulation dysfunction was 4 times higher than that for women with other IVF-ET indications (OR = 4.0, 95

Background The decline in the quantity and quality of mitochondria are closely associated with infertility, particularly in advanced maternal age. Transferring autologous mitochondria into the oocytes of infertile females represents an innovative and viable strategy for treating infertility, with no concerns regarding ethical considerations. As the donor cells of mitochondria, stem cells have biological advantages but research and evidence in this area are quite scarce. Methods To screen out suitable human autologous ooplasmic mitochondrial donor cells, we performed comprehensive assessment of mitochondrial physiology, function and metabolic capacity on a varity of autologous adipose, marrow, and urine-derived mesenchymal stromal cells (ADSC, BMSC and USC) and ovarian germline granulosa cells (GC). Further, to explore the biosafety, effect and mechanism of stem cell-derived mitochondria transfer on human early embryo development, randomized in-vitro basic studies were performed in both of the young and aged oocytes from infertile females. Results Compared with other types of mesenchymal stromal cells, USC demonstrated a non-fused spherical mitochondrial morphology and low oxidative stress status which resembled the oocyte stage. Moreover, USC mitochondrial content, activity and function were all higher than other cell types and less affected by age, and it also exhibited a biphasic metabolic pattern similar to the pre-implantation stage of embryonic development. After the biosafety identification of the USC mitochondrial genome, early embryos after USC mitochondrial transfer showed improvements in mitochondrial content, activity, and cytoplasmic Ca 2+ levels. Further, aging embryos also showed improvements in embryonic morphological indicators, euploidy rates, and oxidative stress status. Conclusion Autologous non-invasively derived USC mitochondria transfer may be an effective strategy to improve embryonic development and metabolism, especially in infertile females with advanced age or repeated pregnancy failure. It provides evidence and possibility for the autologous treatment of infertile females without invasive and ethical concerns.

Studies have shown that letrozole cotreatment can improve clinical outcomes in high and poor responders in GnRH-antagonist protocol. However, whether letrozole is also beneficial to normal responders is not known. To investigate the clinical value of letrozole cotreatment during ovarian stimulation in vitro fertilization for normal ovarian reserve patients who were treated with the GnRH antagonist protocol, we conducted a retrospective study that based data from 1 January to 31 December 2017 for all IVF–ICSI GnRH-antagonist protocols. A total of 252 women who aged <40 years, FSH <10 IU/L on day 3 and antral follicle counting (AFC) >6 were included in the analysis (96 in the letrozole group and 156 in the no-letrozole group). The cumulative live-birth rate was calculated as the first live birth achieved after all cycles having an embryo transfer (cycles using fresh embryos and frozen–thawed embryos) among both groups. The initial gonadotropin (Gn) dosage and total Gn dosage were significantly lower and the number of days of Gn treatment was significantly fewer in the letrozole group than the non-letrozole group (p < 0.05). There were also significant between-group differences in luteinizing hormone, estradiol, and progesterone concentrations; and the number of metaphase II oocytes on the day of human chorionic gonadotropin treatment (p < 0.05). There was a significant difference in the implantation rate between the two groups that the letrozole group higher than the non-letrozole group (39.79 vs. 27.96%, p = 0.006), but there was no significant difference in the cumulative live-birth rate. The combination of letrozole with a GnRH antagonist may have no effect on the clinical pregnancy rate or cumulative live-birth rate in patients with a normal ovarian reserve. However, letrozole may increase the rate of embryo implantation and may reduce the requirement for exogenous gonadotrophins and, consequently, the cost of an IVF treatment cycle. In addition, the decreased estradiol level in the ovarian simulation by letrozole supports letrozole can be a safe solution for fertility preservation in estrogen-related cancer patients.

OBJECTIVE:It is well known that a dual trigger treatment can improve clinical outcomes of in vitro fertilization (IVF) in high or normal ovarian responders. However, it is not clear whether dual triggering also benefits patients with diminished ovarian reserve (DOR). The aim of this study was to investigate whether a dual trigger treatment of gonadotropin-releasing hormone (GnRH) agonist combined with human chorionic gonadotropin (hCG) for final follicular maturation improves the cumulative live birth rate (CLBR) during the GnRH-antagonist cycle in patients with DOR.METHODS:This retrospective study included patients with DOR who received a GnRH-antagonist protocol during IVF and intracytoplasmic sperm injection (IVF-ICSI) cycles at Peking University People's Hospital from January 1, 2017 through December 31, 2017. Oocyte maturation was triggered by GnRH combined with hCG (n=110) or hCG alone (n=71). Embryos were transferred on the third day after oocyte retrieval or during a subsequent freeze-thaw cycle. Patients were followed up for 3 years.RESULTS:The dual trigger treatment did not affect CLBR, which is an overall determinant of the success rate of assisted reproductive technology (ART). Women in the dual trigger group had significantly higher rates of fertilization than those in the hCG group (90.1% vs. 83.9%, P=0.040).CONCLUSION:Dual trigger with GnRH agonist and hCG did not improve CLBR in patients with DOR, but did slightly improve fertilization rate, oocyte count, and embryo quality.

This study investigated the association between house cleaning frequency and the risk of miscarriage in a case control sample of Chinese population. We recruited 59 pregnant women with clinical pregnancy loss as cases and 122 women who chose to conduct induced abortion as controls. All participants were aged 20~40 years and completed a questionnaire of lifestyle exposure with a trained nurse. The effect of frequency of cleaning up on risk of miscarriage was estimated using multivariable logistic regressions, adjusting for potential confounders. In the present study, it was shown that house cleaning of less than twice per week was significantly associated with cough during day or night with odds ratio (OR) of 2.97 (95% CI: 1.36~6.75, p = 0.007), and cough during day or night was significantly associated with risk of miscarriage with OR of 2.69 (95% CI: 1.22~6.02, p = 0.014). Thus, house cleaning of less than twice per week was statistically significantly associated with miscarriage with OR of 3.05 (95% CI: 1.51~6.31, p = 0.002). We found that females who have their house cleaned less than twice per week are at elevated risk for miscarriage. Therefore, the home of pregnant woman should be cleaned at least twice per week in order to avoid miscarriage.

This is a retrospective cohort study included 1021 patients underwent a flexible GnRH antagonist IVF protocol from January 2017 to December 2017 to explore the effect of a premature rise in luteinizing hormone (LH) level on the cumulative live birth rate. All patients included received the first ovarian stimulation and finished a follow-up for 3 years. A premature rise in LH was defined as an LH level >10 IU/L or >50% rise from baseline during ovarian stimulation. The cumulative live birth rate was calculated as the number of women who achieved a live birth divided by the total number of women who had either delivered a baby or had used up all their embryos received from the first stimulated cycle. In the advanced patients (≥37 years), the cumulative live birth rate was reduced in patients with a premature rise of LH (β: 0.20; 95% CI: 0.05–0.88; p =0.03), compared to patients (≥37 years) without the premature LH rise. The incidence of premature LH rise is associated with decreased rates of cumulative live birth rate in patients of advanced age (≥37 years) and aggravated the reduced potential of embryos produced by the advanced age, not the number of embryos.

“Dual triggering” for final oocyte maturation using a combination of a gonadotropin-releasing hormone agonist (GnRHa) and human chorionic gonadotropin (hCG) can improve clinical outcomes in high responders during in vitro fertilization–intracytoplasmic sperm injection (IVF–ICSI) GnRH-antagonist cycles. However, whether this dual trigger is also beneficial to normal responders is not known. We retrospectively analyzed the data generated from 469 normal responders from 1 January to 31 December 2017. The final oocyte maturation was undertaken with a dual trigger with a GnRHa combined with hCG (n = 270) or hCG alone (n = 199). Patients were followed up for 3 years. The cumulative live-birth rate was calculated as the first live birth achieved after all cycles having an embryo transfer (cycles using fresh embryos and frozen–thawed embryos) among both groups. Women in the dual-trigger group achieved a slightly higher number of oocytes retrieved (11.24 vs. 10.24), higher number of two-pronuclear (2PN) embryos (8.37 vs. 7.67) and a higher number of embryos available (4.45 vs. 4.03). However, the cumulative live-birth rate and the all-inclusive success rate for assisted reproductive technology was similar between the two groups (54.07 vs. 59.30%). We showed that a dual trigger was not superior to a hCG-alone trigger for normal responders in GnRH-antagonist cycles in terms of the cumulative live-birth rate.

目的 探讨在受精失败高危因素患者中采用部分卵胞浆内单精子注射(half-ICSI)对临床结局的影响及对体外受精(IVF)受精率的相关因素分析.方法 回顾性分析2017年1月至2018年12月在本院因原发不孕、不孕年限≥5年、不明原因不孕、3次人工授精失败(同时满足两条即可)half-ICSI授精方案的363例患者的临床资料.比较同一患者两种受精方式之间(IVF和ICSI)的受精率、卵裂率、囊胚形成率及妊娠结局.同时采用多因素线性回归分析模型,分析影响IVF受精率的相关因素.结果 363例患者IVF组与ICSI组的受精率为(79.87％,2 384/2 985;88.37％,2 265/2 563)、早期流产率为(12.60％,16/127;25％,20/80),两组比较,差异有统计学意义(P＜0.05).IVF组与ICSI组平均2PN受精数为[(5.54±1.55)个;(5.75±1.28)个]、2PN卵裂数[(5.41±1.45)个;(5.63±1.31)个]、卵裂率(89.26％,2 128/2 384;91.13％,2 064/2 265)、囊胚形成率(40.03％,643/1 606;38.55％,628/1 629),两组比较,差异均无统计学意义(P＞0.05);IVF组与ICSI组平均移植胚胎数[(1.81±0.37)个;(1.82±0.45)个]、胚胎种植率(38.33％,161/420;42.24％,109/258)、临床妊娠率(54.74％,127/232;56.74％,80/141)、双胎妊娠率(26.78％,34/127;23.75％,19/80)、活产率(47.41％,110/232;41.13％,58/141)比较,差异均无统计学意义(P＞0.05).多因素线性回归分析显示,获卵数及促性腺激素(gonadotropin,Gn)总量与IVF受精率呈负相关(回归系数:-0.158,-0.060;P＜0.05).结论 对具有受精失败高危因素的患者,行half-ICSI助孕可以提高部分患者的受精率,其中IVF受精率跟获卵数以及Gn用量成负相关,但对大多数患者而言并没有明显的受益.

The female reproductive toxicity of per- and polyfluoroalkyl substances (PFAS) has raised concerns, but knowledge about their human preconception exposure is limited. In this study, 15 emerging PFAS were identified in follicular fluid samples from healthy women by using high-resolution mass spectrometry, and Cl-substituted perfluoroalkyl ether sulfonates (Cl-PFESAs) including 4:2, 5:2, 6:2, and 8:2 Cl-PFESAs, 4:4 C-8 perfluoroalkyl ether sulfonate (PFESA), C-8 perfluoroalkyl ether carboxylate (PFECA), and C-8 polyether PFECA (Po-PFECA) were detected in over 50% of 28 follicular fluid samples. Ten legacy PFAS were also detected, and the geometric mean concentration of PFOS was the highest (4.82 ng/mL), followed by PFOA (4.60 ng/mL), 6:2 Cl-PFESA (1.09 ng/mL), PFHxS (0.515 ng/mL), PFNA (0.498 ng/mL), and C-8 PFECA (0.367 ng/mL). The blood-follicle transfer efficiencies for PFCAs decreased with increasing chain length (0.96 for PFHpA, 0.56 for PFTriDA), and the transfer efficiencies of C-8 PFECA (0.78) was significantly higher than that of PFOA (0.76). The transfer efficiencies of 4:2 Cl-PFESA (0.73), 6:2 Cl-PFESA (0.75) and 8:2 Cl-PFESA (0.91) were significantly higher than that (0.70) of PFOS (p = 0.028, 0.026 and 0.002, respectively). This study constitutes the first report of the human oocyte exposure to emerging PFAS and their blood-follicle transfer abilities.

目的 探讨信息化管理对辅助生殖术后患者随访工作的重要性.方法 统计随访过程中随访延误、错访、漏访和失访的发生率,比较人工管理模式和电子化信息管理模式下随访工作效率及质量控制的差别.结果 两种管理模式下随访的IVF、FET和IUI三组病例,其随访延误率、错访率、漏访率和失访率的差别均有统计学意义(P＜0.05).结论 采用电子化信息管理模式,显著提高随访工作效率及质量控制.

目的:基于电子病历系统设计辅助生殖技术手术通知单以及手术通知流程,实现生殖中心医疗工作的统一管理.方法:确定手术通知单内容;制定获取部位:决定触发节点;设计手术排序的原则;设计列表界面及打印界面;制定手术通知工作流程.结果:手术通知单内容包括患者基本信息和临床信息,信息自动获取,填写扳机方法和时间生成手术通知单,形成固定手术通知流程.结论:流程设计可以自动获取基本信息:保证关键数据的录入;数据自动获取;手术安排自动化;手术列表信息完备,以方便护士和实验室人员安排工作;保证医疗安全.