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1Relationship between serum tumor markers and EGFR gene mutation in NSCLC based on logistic regression and ROC curve analysis

JI Hai-fen,LUO Rui,Chen Yu

目的:采用logistic回归和受试者特征曲线(ROC)方法评价血清肿瘤标志物与非小细胞肺癌(NSCLC)中表皮生长因子受体(EGFR)基因突变的相关性.方法:收集肺腺癌住院病人128例,记录病人是否存在吸烟史,并收集病人初诊时静脉血5 mL,分离血清3 mL,检测病人血清肿瘤标志物.按照EGFR基因检测结果分为EGFR基因突变组和EGFR基因非突变组,比较2组病人的一般临床特征,基于logistic回归模型,绘制ROC曲线,计算曲线下面积(AUC)评价各指标及联合诊断的价值.结果:EGFR的突变与病人的性别、吸烟史、血清鳞状细胞癌抗原(SCC)以及血清糖类抗原(CA125)水平相关(P<0.05～P<0.01),NSCLC病人中女性发生突变频率高于男性(P<0.01),有吸烟史病人EGFR的突变频率低于无吸烟史病人(P<0.01),血清SCC水平阴性病人EGFR的突变频率高于血清SCC水平阳性(P<0.01),血清CA125阴性EGFR的突变频率高于CA125阳性病人(P<0.05).在血清肿瘤标志物中对EGFR基因突变诊断价值具有统计学意义的是性别、CA125、SCC以及病人的吸烟史(P<0.05),指标联合诊断中,性别+SCC、性别+CA125+SCC、性别+SCC+吸烟史、CA125+SCC+吸烟史、性别+CA125+SCC+吸烟史五者的AUC均>0.75(P<0.05),具有较高的诊断价值.结论:血清肿瘤标志物中SCC和CA125与NSCLC中发生EGFR突变存在着较好的相关性,与其他临床资料(性别、吸烟史)相结合增强了EGFR的预测鉴别能力,值得临床进一步推广应用.

2022Journal of Bengbu Medical College（2022）

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2miR-1251-3pはHE4を負に調節し、卵巣癌細胞株COC1の増殖と浸潤を抑制する。【JST・京大機械翻訳】

Li Feifei,Deng Qingmei, Cai Yunshuo,Ji Haifen

2020

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3miR-1251-3p negatively regulates HE4 inhibits the proliferation and invasion of ovarian cancer cell line COC1

Fei-fei Li,Qing-mei Deng, Yun-shuo Cai,Hai-fen Ji

目的:探讨miR1251-3p负调控HE4对卵巢癌细胞株COC1的增殖和侵袭的影响.方法:实验分为空白组、阴性对照组、模拟物组(mimic)和抑制剂组(inhibitor).采用qPCR、划痕实验和Transwell小室实验,检测miR-1251-3p在卵巢癌细胞株COC1细胞株侵袭转移中的作用.结果:miR-1251-3p与HE4的3'UTR区域有结合位点.与卵巢癌细胞株COC1空白组和阴性对照相比,转染miR-1251-3p mimics会抑制增殖和侵袭,而转染miR-1251-3p inhibitor可以促进卵巢癌细胞COC1的增殖和侵袭.结论:miR-1251-3p负调控HE4抑制卵巢癌细胞株COC1的增殖和侵袭.

2020Journal of Hunan Normal University(Medical Science)（2020）引用：1

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4Application value of the joint detection of serum carcinoembryonic antigen and lysophosphatidic acid in patients with non-small cell lung cancer

Rui LUO,Hai-feng JI,Xiao-qin YU

目的:探讨血清癌胚抗原(CEA)与溶血磷脂酸(LPA)在非小细胞肺癌病人中的应用价值.方法:选取84例非小细胞肺癌病人(非小细胞肺癌组)、84例肺炎病人(肺炎组)和84名健康体检者(对照组),分别测定和比较3组人群的血清CEA和LPA水平.分析血清CEA和LPA联合检测对非小细胞肺癌的诊断价值.结果:非小细胞肺癌病人血清CEA和LPA水平显著高于肺炎病人和健康体检者(P<0.01);腺癌组病人血清CEA阳性率(62.5％)显著高于鳞状细胞癌组和组织未分型组病人(P<0.05),另外LPA在3组非小细胞肺癌病人血清中的阳性率相当(45.00％～54.55％),3组间差异无统计学意义(P>0.05);CEA与LPA在诊断非小细胞肺癌中的效能结果显示,血清CEA单独检测阳性率为31.35％,LPA单独检测阳性率为30.16％,两者联合检测阳性率为38.89％.血清CEA准确度为67.86％,特异度为74.60％,灵敏度为50.40％;LPA准确度为64.68％,特异度为72.62％,灵敏度为42.86％;两者联合检测准确度提高到69.44％,灵敏度提高到60.71％,特异度也达到了70.24％.结论:血清CEA和LPA联合检测有利于提高非小细胞肺癌诊断的灵敏度和准确度,值得临床推广.

2018Journal of Bengbu Medical College（2018）引用：1

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5Distribution of autoantibody spectrum in serum of diabetic patients Application and study of early diagnosis

Rui Luo,Hai-fen Ji

目的评价联合检测血清中谷氨酸脱羧酶抗体(GADA)、胰岛细胞抗体(ICA)、蛋白酪氨酸磷酸酶抗体(IA-2A)、胰岛素抗体(IAA),锌8抗体(ZnT-8A)在糖尿病患者中的分布及对糖尿病早期诊断的应用价值研究.方法收集2016年1月至2017年8月合肥市第一人民医院就诊糖尿病患者157例,作为糖尿病组;其中Ⅰ型糖尿病患者48例,设为Ⅰ型糖尿病组;Ⅱ型糖尿病患者109例,设为Ⅱ型糖尿病组,同期收集同龄健康体检者血清(非糖尿病患者)194份,作为正常对照组.使用免疫印迹试剂法对各组糖尿病抗体(GADA,ICA,IA-2A,IAA,ZnT-8A)进行检测,并对检测结果进行统计分析.结果糖尿病抗体谱在Ⅰ型糖尿病组的检出率明显高于Ⅱ型糖尿病组,且差异具有统计学意义(P＜0.05),Ⅰ型糖尿病组的检出率分别为GADA (54.17),ICA(27.08％),IA-2A(6.25％),IAA(10.42％),ZnT-8A(12.5％).在Ⅰ型糖尿病组中糖尿病抗体谱以GPDA+ ICA阳性最为常见,阳性率高达20.83％,其次是GPDA+ZnT-8A(12.5％),与其他模式间差异存在统计学意义(P＜0.05).结论 GADA、ICA、IA-2A,IAA,ZnT-8A的测定对糖尿病具有重要的应用价值以及临床意义,联合检测可以有效的提高阳性率,以免漏诊.

2018Diabetes World（2018）引用：1

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6The Clinical Value of Combined Detection of Serum Tumor Markers in Diagnosis of Liver Cancer

叶芳,计海芬,罗锐,汪学龙

Objective To explore the clinical value in combined detection of the serum tumor markers on patients with hepatocellular carcinoma.Methods56 cases primary liver cancer of the hospitalized patients,32 cases of other liver diseases, and34 healthy persons were collected. Carcinoembryonic antigen (CEA), serum malignant tumor growth factor (TSGF), glutamate based transpeptidase (GGT), alpha fetoprotein (AFP)and fucosidase (AFU) were determined.Results Indexes AFP, TSGF, AFU, GGT and CEA in patients with primary liver cancer were higher than those with benign liver diseases and normal control (P<0.05). The sensitivity and specificity of combined detection were higher than those of the single test (P <0.05).Conclusion The combined detection of serum tumor markers of liver cancer can effectively improve the accuracy of diagnosis, and provide important basis for the differential diagnosis of hepatocellular carcinoma.

2016引用：8

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7Clinical significance of NLR detection in tumor patients with pathogens infection

邓庆梅,李飞, 赵华,计海芬,叶芳

Objective To discuss the diagnostic value of peripheral blood neutrophil-to-lymphocyte ratio (NLR) in the tumor pa-tients with pathogens infection. Methods The clinical data of 43 tumor patients with pathogens infection ( the infection group) and 43 tumor patients without pathogens infection ( the non-infection group) ever treated in our hospital from Apr 2014 to Jan 2016 were retrospectively ana-lyzed. The variability and correlation of leukocyte count, neutrophils count, lymphocyte count, NLR, hemoglobin ( Hb) level and serum al-bumin ( ALB) level in the two groups were statistically analyzed and compared with the healthy controls. The risk factors of pathogens infec-tion in the tumor patients were analyzed by binary logistic regression, and the diagnostic value of NLR in the tumor patients with pathogens in-fection were evaluated by receiver-operating characteristic ( ROC) curves. Results NLR of the infection group was significantly higher than those of the non-infection group and healthy controls, and NLR of the non-infection group was also significantly higher than that of healthy controls (P=0. 000). Leukocyte count and neutrophils count of the infection group were significantly higher than those of the non-infection group and healthy controls (P =0. 000, P =0. 001), while no significant difference was found between the non-infection group and the healthy controls (P=0. 240, P=0. 666). Lymphocyte count, Hb and ALB levels of the infection group were significantly lower than those of the non-infection group and healthy controls (P=0. 000, P=0. 000, P=0. 003), and those of the non-infection group were also significant-ly lower than those of healthy controls (P=0. 000, P=0. 000, P=0. 000). NLR of the tumor patients was negatively associated with their serum ALB levels, Hb levels and lymphocyte count (r= -0. 530, r= -0. 216, r= -0. 740;P=0. 000, P=0. 046, P=0. 000), and was positively associated with their neutrophils count (r=0. 604, P=0. 000). Logistic regression analysis showed that the increase of NLR and the decrease of ALB were independent risk factors of the tumor patients with pathogens infection (P=0. 001, P=0. 023). ROC curves resul-ted that area under the curve (AUC) of NLR was 0. 864 (P=0. 000), and when the NLR value being 4. 19 and 4. 62, the NLR sensitivity of infection diagnosis were 88. 39% and 83. 7%, the NLR specificity were 69. 77% and 74. 4%, respectively, with the best diagnostic val-ue. The AUC of neutrophils count was 0. 647 (P=0. 018), and the leukocyte count showed no diagnostic value for infection (P=0. 237). Conclusion The peripheral blood NLR shows diagnositic value in the tumor patients with pathogens infection, and it also has correlation with the ALB and Hb levels.

2016引用：4

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