目的 探讨EAMG大鼠肾上腺的免疫相关性,及强的松的治疗机制,以阐明EAMG的发病机制.方法 36只Lewis大鼠随机分为模型组、对照组、强的松组3组,每组12只.观察各组EAMG大鼠Lennon评分,并通过ELISA法检测EAMG大鼠血清AchR-Ab含量水平,通过PCR法检测肾上腺中IFN-γ mRNA、TGF-β mRNA、TNF-α mRNA、IL-2 mRNA、IL-4 mRNA、IL-17 mRNA的表达.结果 与对照组比较,模型组大鼠肾上腺中 IFN-γ mRNA、TGF-β mRNA、IL-4 mRNA表达减少,TNF-α mRNA、IL-17 mRNA的表达增加,且有显著差异;与模型组比较,强的松组大鼠肾上腺中IFN-γ mRNA表达减少,TGF-β mRNA、IL-4 mRNA、TNF-α mRNA、IL-4 mRNA、IL-17 mRNA的表达增加;EAMG大鼠肾上腺中没有IL-2 mRNA的表达.结论 强的松能有效改善EAMG大鼠Lennon评分,降低EAMG大鼠血清AchR-Ab水平,保护EAMG大鼠肾上腺细胞TGF-β mRNA、IL-4 mRNA的表达,减少肾上腺细胞TNF-α mRNA、IL-17 mRNA、IFN-γ mRNA的表达,这可能是其治疗MG的作用机制之一.

Objective To investigate the association between depression behavior and the expression of calreticulin precursor, tropomyosin I, milogen activated prolein kinase 1 and dual specificity mitogen-activated protein kinase 1 in spontaneous diabetes mellitus rats. Methods Twenty adult male healthy rats (control group) and 40 spontaneous diabetic rats were studied. Forty spontaneous diabetic rats were further divided into diabetes group and diabetic depression group, and the latter was to construct depression model. The depressive behavior of the 3 groups was measured by sucrose water consumption test and wilderness test. The expression level of calreticulin precursor, tropomyosin 1, milogen activated prolein kinase 1, and dual specificity mitogen-activated protein kinase 1 in the hippocampus of the 3 groups was measured by western blot. Results In the 15 th day and 22 nd days of stress, the amount of sucrose water drinking of the rats in diabetic depression group was significantly lower than that of the control group and the diabetes group (P < 0.05) , and there was no significant difference between the control group and the diabetic group (P> 0.05). The score of field test and vertical score in the diabetic depression group were significantly lower than those of the control group and the diabetes group (P < 0.05) , but there was no significant difference between the control group and the diabetic group (P> 0.05). The expression of calreticulin precursor, tropomyosin 1, milogen activated prolein kinase 1, and dual specificity mitogen-activated protein kinase 1 in the hippocampus of diabetic depression group was significantly lower than that in the control group and the diabetic group (P < 0.05) , and there was no significant difference between the control group and the diabetic group (P> 0.05).Conclusions The depressive behavior of spontaneously diabetic rats is associated with the decrease of the expression level of calreticulin precursor, tropomyosin 1, milogen activated prolein kinase 1, and dual specificity mitogenactivated protein kinase 1 in the hippocampus of rats.