Objective:To investigate the effect of platelet antibody screening and cross-matching on platelet transfusion in patients with acute immune thrombocytopenia (AITP).Methods:A total of 97 patients with AITP were screened for antibody, of which 33 (34.02%, 33/97) were antibody positive and 64 (65.98%, 64/97) were antibody negative. There were no significant differences in platelet infusion times and antibody positive rate between observation group and control group ( t=0.98, χ2=0.33, both P values>0.05).The effective rate of the observation group was higher than that of the control group(93.88% vs. 64.58%, χ2=12.71, P<0.05).Compared with 1 h after infusion, the CCI value of observation group was higher than that of control group at 1 and 24 h after infusion [×10 9/L: (12.60±2.64) vs (8.61±1.48), (7.37±1.55) vs (5.09±0.85); t values were 9.21 and 8.96, respectively, both P values<0.05].After infusion, the levels of prothrombin time(PT) in AITP patients in the observation group and control group both increased, and thrombin time(TT) and activated partial thromboplastin time(APTT) levels decreased.The coagulation function indexes of the observation group were better than those of the control group[S: (12.60±2.64)vs(8.61±1.48), (7.37±1.55)vs(5.09±0.85); t values were 9.21, 8.96, both P values <0.05)].The incidence of adverse reactions in the observation group was 12.5%, which was lower than 32.65% in the control group( χ2=4.525, P<0.05). Conclusion:Platelet antibody screening and blood cross-matching can effectively improve the transfusion effect, coagulation function and blood transfusion safety in patients with acute AITP.