To understand codon usage features of ICE1 gene in radish,the sequence of ICE1 was analyzed via programs of CUSP and Codon W,and it was also compared with other 10 plant species and genomes.The results showed that ICE1 in radish was bias towards the synonymous codons with A and T at third codon position.According to the RSCU (Relative Synonymous Codon Usage),12 codons were high-frequency.Compared with the codon usages of other radish genes,bias towards of 19 codons were the same.There were some differences in codon bias of ICE1 among different species.The cluster tree of ICE1 based on CDS revealed a better evolutionary relationship among different species than the result analyzed by RSCU.The differences of codon usage between ICE1 and rice genome was larger than that between ICE1 and some cruciferae plant genomes(Arabidopsis,cabbage).This means something should be done to ICE1 to heterologous expression of ICE1.This study not only preliminarily reveals the codon usage pattern of ICE1 of radish,but also provides a certain theoretical basis to further molecular genetic study.

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