Mango (Mangifera indica L.), a climacteric fruit of significant global economic value, suffers from rapid postharvest deterioration driven by complex hormonal and transcriptional networks. While chitosan coating is known to extend mango shelf-life, the precise molecular mechanisms, particularly its interplay with key ripening hormones like abscisic acid and ethylene, and the role of transcriptional regulators, remain poorly understood. We hypothesized that chitosan delays ripening by reprogramming abscisic acid and ethylene signaling pathways and associated transcriptional networks. To test this, we employed an integrative multi-omics approach combining transcriptomics and metabolomics to elucidate the molecular basis of chitosan-mediated ripening delay in mango fruit. Our findings reveal that chitosan orchestrates a dual hormonal attenuation associated with suppression of abscisic acid biosynthesis (NCED3, ABA2) and ethylene signaling (ETR1, EIN3/EIN4) while enhancing abscisic acid catabolism (CYP707A1/4). Crucially, γ-aminobutyric acid (GABA) accumulation antagonized abscisic acid to uncouple starch-to-sugar conversion from ripening progression, correlating with retained firmness and chlorophyll levels. Furthermore, we identified key transcription factors, WRKY53 and bZIP/RF2b, likely acts as central hubs modulating abscisic acid-ethylene crosstalk and cell wall integrity. This knowledge provides novel mechanistic insights into chitosan action and offers a foundation for developing targeted strategies to optimize postharvest preservation in climacteric fruits by synchronously modulating Abscisic acid-ethylene crosstalk, GABA-mediated metabolic reprogramming, and key TFs.

课程思政是近年来各类教育的热点问题,该文针对农林类专业教学如何融入课程思政教育及探究课程思政如何切入教学的问题,从教学理念和目标的思政,教学方案和方法的思政,内容和案例的思政,内涵和主旨的思政等方面提出农林类专业教学融入课程思政的4个切入点;围绕"农林类专业课程思政如何开展"这一核心问题,提出学院整体规划—组队研讨实践—实行由点到面—全面推进建设的课程思政推进路线;从科学完善的整体规划、教研团队的打造、协同创新体系的3个维度,以课程思政试点建设为突破口,带动全课程思政,进而促进专业思政,实现农林类专业内涵建设;通过分组合作,订立条规计划,探究融合等多种教学方法和实践教学,从而实现专业教学与价值引导的有机融合,为农林类专业课程切入思政教育提供一定的模式借鉴.

本文通过分析广西现有乡村文化,探索乡村文化的内容及传承方式,找到广西乡村文化传承发展存在的问题,提出课堂教育教学,课外兴趣拓展,校园文化建设,节假日游学等方面融入乡村文化等策略来拓宽乡村文化传承发展路径,为义务教育融入地方特色文化提供参考;提升义务教育阶段对乡村文化的重视程度,丰富素质教育内涵,扩大乡村文化在校园中的影响,促进乡村文化的繁荣与兴盛,让学子们真正"望见青山绿水,铭记乡愁".

为提高地方应用型本科院校分子生物学课程教学质量,探讨了分子生物学课程教学改革策略.针对当前分子生物学理论课和实验课教学中存在的课程内容相对晦涩难懂、学生实践和创新能力薄弱等问题,提出了解决措施:从课堂教学方面,提升教育教学理念,优化教育教学体系,改善教学方式和教学手段,改进学习评价方式;从实验教学方面,创建良好的实验条件,合理安排实验进度,探索先进的实验方法,采取有效的实验评价方式,以期提高分子生物学教学效果和质量,培养实践能力强的创新型应用人才.

近几年,高校文化创意产品的研发体系越来越成熟,其产品的研发与使用在老师和学生的日常生活中扩展开来,其中包括学生在学习上、生活上,以及对文化用品的需求等.然而,我国高校文化创意产品样式单一、结构简单,缺少鲜明的地域文化特色,为了解决这些问题,文章分析校园文化创意产品设计存在的问题,将地域文化作为一个新的元素去发展创新校园文化产品的优势,提出基于地域文化的校园文化创意产品设计思路.以期丰富校园文化创意产品的内涵,更好地传承地域文化并促进经济的发展.

In this study, the effects of pectate and pectate lyase (PL) on nutritional quality of tomato fruits were investigated. During postharvest storage, pectate increased the fruit firmness and changed biochemical indicators such as water-soluble pectin (WSP) content and peroxidase activity (POD), simultaneously. RNA-Seq analysis and functional annotation showed that the majority of differentially expressed genes (DEGs) distributed in amino acid metabolism, carbohydrate metabolism, signal transduction pathways in either pectate or PL groups. In view of the opposite effects of pectate and PL on fruit postharvest physiology, 56 DEGs with opposite expression trend were considered to be involved in fruit softening. Subsequently, the qRT- PCR results indicated that transcription of SlPME2.1, SlCHI19, SlMal d1 and SlPR-4B related to cell wall tissue or biogenesis as well as pathogenesis were regulated by pecate and PL. The results help clarify the mechanism of pectate treatment on improving the softening of postharvest tomato fruits.

为探究Sl-miR6027在果实成熟过程中的功能,本研究利用生物信息学方法,分析了Sl-miR6027在番茄果实发育过程中的表达模式,同时对Sl-miR6027启动子中存在的顺式作用元件和Sl-miR6027的靶基因进行预测;依据Sl-miR6027成熟序列,设计STTM寡核苷酸序列,构建pCambia-STTM6027沉默载体;采用农杆菌介导法转化番茄,通过PCR筛选阳性转化植株并进行初步的表型分析.结果 表明:Sl-miR6027在绿熟期表达量最高;Sl-miR 6027启动子中存在20个光应答元件和一些非生物胁迫应答元件;Solyc09g098130为Sl-miR6027潜在的靶基因,二者在果实成熟不同时期表达量呈负相关,且其表达水平受到光照的调节;依据Sl-miR6027成熟序列成功构建出pCambia-STTM6027沉默载体;pCambia-STTM6027转基因番茄表型分析表明Sl-miR6027参与调节红熟期番茄果实颜色.上述结果初步表明Sl-miR6027参与调节番茄果实成熟过程.

研究番茄Sl-miR482在果实成熟阶段特殊的表达模式,对其功能进行生物信息学分析并构建STTM沉默载体,为深入阐明Sl-miR482在番茄果实成熟软化中的作用机制奠定基础.采用定量PCR进行基因表达检测;利用启动子分析软件和靶基因预测网站进行生物信息学分析;利用PCR扩增及酶切、连接反应进行STTM载体构建,并转化农杆菌.Sl-miR482在开花期表达量逐渐上升,到未成熟绿期表达量达到最高峰,随后逐渐下降.进一步生物信息学分析结果表明,Sl-miR482启动子中存在乙烯应答元件等.靶基因预测网站结果显示,番茄果胶裂解酶Sl-PL13为Sl-miR482的靶基因;基因表达分析结果表明,Sl-PL13与Sl-miR482表达量在果实成熟不同阶段呈现此消彼长的关系.利用PCR扩增及酶切连接方法,成功构建STTM482沉默载体并转化农杆菌.Sl-miR482在果实成熟软化过程中发挥重要作用.

Radiotherapy is one of the most common and effective treatments for localized cancer. However, radiotherapy kills tumor cells while causing damage to surrounding normal cells. Enhancing the radiation sensitivity of tumor cells and reducing the radiation damage to normal cells is a difficult problem. Here, we find that the expression of a human microRNA (miRNA), hsa-miR-222, is upregulated in response to ionizing radiation. TargetScan analysis shows that the 3' UTR of CD47 is potentially targeted by miR-222. This prediction was validated by luciferase reporter and mutation assays. It was demonstrated that miR-222 negatively regulates CD47 expression at mRNA and protein levels, and overexpression of the miR-222 enhances cancer cell radiosensitivity by the CD47-pERK pathway in cancer cells. Our findings enrich the complex relationship between miRNA and CD47 in irradiation stress and shed light on the potential of miRNAs both for direct cancer therapeutics and as tools to sensitize tumor cells to radiotherapy.