The present study was designed to evaluate the effects of astragalan on low temperature preservation of boar semen and subsequently find out a natural cryoprotectant which can protect improve the low temperature preservation of boar semen.Boar semen was preserved at a low temperature(4 ℃)for 7dusing the modified buffer supplemented with various concentrations(0,0.10,0.20,0.30 and 0.40g/L)of astragalan and data on sperm viability,motility,abnormality,acrosomal integrity and plasma membrane integrity were compared between the treatments and the control.The results showed that 0.10g/L astragalan didnu0027t significantly(P0.05)alter the quality of boar semen stored at a low temperature;0.40g/L astragalan significantly(P0.05)reduced the above-mentioned sperm parameters;0.20 and 0.30g/L astragalan demonstrated significant(P0.05)improvement on the sperm measurements;Of all groups,0.30g/L astragalan indicated the best improvement effect on boar spermatozoa.In summary,under the conditions of this study feasible concentration of astragalan significantly increases the quality of boar semen conserved under low temperature(4℃),and the addition of 0.30g/L astragalan in the semen extender could be optimal to the low temperature preservation of boar spermatozoa.

为了改善猪精液低温保存方法,通过在改进的猪精液低温稀释液中添加不同浓度(0,0.50,1.00,1.50,2.00 mg/mL)的水蛭素,研究在4℃低温保存条件下其对猪精液品质的影响.试验采用PI荧光染色检测精子活率,利用计算机辅助精液分析仪(CASA)检测精子活力,Diff-Quik染色检测精子畸形率,考马斯亮蓝染色检测精子顶体完整率及采用低渗肿胀法(HOST)检测质膜完整率.猪精液保存7d,每天检测上述指标,试验重复5次.结果表明:稀释液中添加一定浓度的水蛭素在一定时间内有利于维持精子的活率、活力,降低精子的畸形率,提高精子的顶体完整率和质膜完整率,并且水蛭素浓度越高,保存效果越好.因此,在本试验条件下,2.00 mg/mL是水蛭素在猪精液低温保存时的最佳添加浓度.