结直肠癌（CRC）是全球最普遍和最致命的实体恶性肿瘤之一 [1] ,也是全球癌症相关死亡的第二大常见原因，终生风险约为4%至5% [2] 。CRC的发病率和死亡率正在迅速增加 [3] 。本实验通过二维色谱质谱联用技术和免疫印迹探究大鼠LIM半胱氨酸丰富域蛋白1（LMCD1）在癌组织和癌旁组织中的差异表达及意义，报道如下。

结直肠癌是世界第三大最常见癌症[1]。其发病率和死亡率在全球范围内不断增加，仅2020年全球结直肠癌新发病例为193.16万，死亡病例93.52万，因此迫切需要降低发病率和死亡率[2]。本实验通过二维色谱质谱联用技术（LC-MS）及免疫印迹探究TOMM40在结直肠癌组织及癌旁组织的表达及意义，为结直肠癌的筛查和诊断提供一个新的方向。1材料与方法1.1一般资料本研究所选用的结直肠癌及配对癌旁组织标本经病理医师确认均为DukesB期腺癌，共16对，来源于胃肠外科手术切除，标本采集时间段为2018年1月到2020年12月，所有患者均签署了知情同意书，且保证术前没有经过任何方式治疗。

叶酸参与生物系统RNA和DNA的合成及氨基酸的代谢,怀孕期间由于细胞生长和增殖非常迅速,因而需要更多 [1] 。摄取量不够或遗传因素导致叶酸的利用能力降低,都能引起机体叶酸缺乏,亚甲基四氢叶酸还原酶（MTHFR）作为叶酸代谢通路中的一个关键酶,它包含11个外显子及10个内含子,位于1号染色体1p36.3上 [2] 。

酪氨酸3-单加氧酶/色氨酸5-单加氧酶活化蛋白（YWHAE）,也称为14-3-3ε是14-3-3家庭的成员。这个家族的成员是约30kDa酸性多肽,具有高度调节多种细胞功能的保守序列,包括细胞周期调节、信号转导、粘附和恶性转化 [1] 。14-3-3蛋白是真核生物体内存在的可溶性酸性蛋白,它通过与靶蛋

中性粒细胞是机体的免疫防御系统中的一条非常重要的防线,它参与机体的免疫反应主要是在病原体感染时,通过吞噬、脱颗粒来抵抗侵袭,形成吞噬体后,在内部通过NADPH [1] 形成具有高浓度的活性氧（reactive ox-ygen species,ROS）和抗菌蛋白可以用来杀灭病原微生物,而在2004年,Brinkmann等 [2] 首次研究证明,当受到病原体刺激后,中

Objective To study the expression of differentially expressed protein periostin in colorectal cancer tissues and adjacent tissues,and to explore the clinical significance of the increased expression protein periostin in colorectal cancer.Methods Differential expression of Periostin was screened by two-dimensional chromatography and mass spectrometry technique,and the differential expression of Periostin was validated by immunohistochemistry technique.Results 13 protein-adjusted proteins from the 34 differential proteins in colorectal cancer tissues and adjacent tissues were screened.Conclusion The expression of periostin in colorectal cancer tissue is higher than that of normal carcinoma,which can be used as a supplementary index for colorectal cancer diagnosis.

目的测定vinculin在大肠癌及肾癌组织中的表达情况,探究其在大肠癌及肾癌发生发展中的意义。方法用二维液相色谱-质谱联用技术对20例DukesB期大肠癌患者的癌组织及癌旁组织、18例透明细胞肾癌患者的癌组织及癌旁组织中vinculin表达水平进行检测,并通过免疫印迹实验进一步验证。结果 vinculin在大肠癌组织中的表达水平低于其癌旁组织,相反,在肾癌组织中表达较其癌旁组织高。结论 vinculin在大肠癌及肾癌的发生发展中起一定作用。

Objective To construct a prokaryotic cloning vector of invasion- associated protein (iap) gene p60 of Listeria monocytogenes. Methods Under gradient PCR amplification conditions, Listeria monocytogenes 54002-4 strain iap gene was amplified with our in-house primers. BamH Ⅰ and Xho Ⅰ sites were induced to 5' and 3' terminals of iap gene, respectively. PCR products were then analyzed and collected by agarose gel electrophoresis. Purified PCR product was connected with the vector pMD18-T. The recombinant plasmid was transformed into Escherichia coli JM109 competent cells, with transformation effect observed after 1mmol/L IPTG induction for 4-6 h. Results Colorless bacterial colony was cultured and verified to produce positive recombinant plasmid pMD18-T-Iap by PCR and nucleotide sequencing. Conclusions PCR optimization conditions and methods are established through gradient PCR. Iap gene cloning vector is obtained after transformation.

Objective To prepare monoclonal antibodies against Listeria monocytogenes and to creat a colloidal gold immunochromato graphy assay fordetecting Listeria monocytogenes.Methods Purified Listeria monocytogenes was used as antigen to immune Balb/c mice.Monoclonal antibodies against Listeria monocytogenes were prepared by normal hybridoma technology.Colloidal gold was coupled with the antibodies against Listeria monocytogenes and Listeria monocytogenes was detected by immunochromatography assay in this study.Results Colloidal gold was prepared by citrate-natrium method and the best compatible protein concentration was measured as 25 ug/ml,therefore,gold immunochromato graphy assay for detection was founded.Conclusion The detection method is aconvenient,quick,effective technology for Lizteria monocytogenes.