Protein tyrosine phosphatase non-receptor type 21 (PTPN21) has been recognised as a new tumour-associated protein that is implicated in diverse tumours. However, the correlation between PTPN21 and glioma remains unaddressed. This investigation focused on the relevance of PTPN21 in glioma. The Cancer Genome Atlas (TCGA) analysis identified PTPN21 as being up-regulated in glioma tissue. The elevation of PTP21 in glioma was validated by evaluating clinical specimen. Kaplan-Meier plot analysis revealed that a high PTPN21 level predicted poor survival rate in glioma patient. Silencing of PTPN21 produced remarkable anticancer effects in glioma cells including proliferation inhibition, cell cycle arrest, metastasis suppression and enhanced chemosensitivity. Mechanistic studies uncovered that PTPN21 contributes to mediation of the phosphatidyl-inositole-3 kinase (PI3K)/AKT pathway via the regulation of epidermal growth factor receptor (EGFR). Restraint of EGFR diminished PTPN21 overexpression-induced promoting effect on PI3K/AKT pathway. Reactivation of AKT reversed PTPN21 silencing-evoked antitumor effect. The tumorigenic potential of PTPN21-silenced glioma cells in vivo was markedly compromised. In summary, this study demonstrates that silencing of PTPN21 produces remarkable anticancer effects in glioma by restraining the EGFR/PI3K/AKT pathway.

目的 探讨不依赖于多普勒超声引导的锁骨下静脉穿刺置管方法.方法 回顾性分析2014年1月至2019年2月西安医学院第二附属医院神经外科需进行锁骨下静脉置管术的128例患者的临床资料,患者均采用锁骨下静脉穿刺法静脉置管.观察患者的穿刺成功率及不良反应发生情况.结果 128例患者在不依赖超声引导下锁骨下静脉置管过程中,一次穿刺成功95例(74.22％);经反复穿刺多次获得成功24例(18.75％);4例(3.12％)反复调整不成功,更换穿刺部位或超声引导下穿刺成功;穿刺不成功停止穿刺5例(3.91％).128例患者穿刺置管中误穿动脉12例(9.38％),局部血肿形成6例(4.69％),气胸2例(1.56％),短期内(1周内)堵管17例(13.28％),菌血症2例(1.56％).结论 锁骨下静脉穿刺置管是神经外科患者常用、安全、有效的血管通路.掌握正确的锁骨下静脉盲穿方法是术者提高穿刺成功率、减少并发症发生的保障,不依赖多普勒超声适时引导减轻了患者的经济负担,值得临床推广.

Cognitive impairments are associated with advancing age. Trans-cinnamaldehyde (CIN) and ellagic acid (ELA) have multiplex activities to reduce various age-related cognitive disorders. In this study, we investigated the effects of these compounds separately or in combination on the cognitive outcomes, mitochondrial function, and inflammatory and apoptotic mediators in aged male Wistar rats. Thirty-two old (22 months old) and eight young (5 months old) rats were randomly allocated to five groups of young control, aged control, ELA-aged, CIN-aged, and ELA + CIN-aged. ELA (15 mg/kg, orally) and CIN (50 mg/kg, intraperitoneally) separately or in combination were administered for 1 month in aged animals. Spatial memory and cognitive activity were evaluated by the Barnes maze and novel object recognition tests. Mitochondrial function (its reactive oxygen species [ROS], mitochondrial membrane potential and ATP level), pro-inflammatory cytokines such as interleukin (IL)-1β and IL-6 and pro-apoptotic caspase 3 and Bax, and anti-apoptotic Bcl2 levels and their ratio were assessed in the prefrontal cortex. Behavioral results revealed that CIN separately or in combination with ELA significantly alleviates aging-induced memory impairment. Moreover, co-administration of agents effectively decreased inflammatory cytokines, cleaved-caspase 3, Bax and Bax/Bcl2 levels, mitochondrial ROS production, and mitochondrial membrane depolarization and increased Bcl2 and ATP level as compared with untreated aged control rats. Combination therapy was greater than those of individual treatments in all parameters. Therefore, combination therapy with CIN and ELA improved aging-induced cognitive impairment through anti-inflammatory, anti-apoptotic, and mitochondrial-boosting effects in aged rats.

目的 分析显微血管减压术中三叉神经根部浅表电凝治疗三叉神经痛的效果.方法 选取164例三叉神经痛患者随机分为两组,对照组给予常规显微血管减压术治疗,观察组给予显微血管减压术且术中对三叉神经根部浅表电凝.比较两组手术指标、不同时刻疼痛评分、临床疗效、术后1个月内并发症发生率及随访6个月复发率.结果 两组的手术时间、术中出血量相比差异均无统计学意义(P＞0.05),观察组住院时间短于对照组(P＜0.05);两组疼痛评分均随着时间的延长显著下降(P＜0.05),术后观察组的疼痛评分均低于对照组(P＜0.05);观察组总有效率高于对照组(P＜0.05);两组术后1个月内听力下降、脑脊液漏、面瘫的发生率及复发率相比差异均无统计学意义(P＞0.05),观察组面部麻木发生率高于对照组(P＜0.05).结论 显微血管减压术中三叉神经根部浅表电凝治疗三叉神经痛可以缩短住院时间,减轻疼痛感,提高临床疗效,虽然可增加面部麻木发生率,但可快速恢复.

目的:探讨康复治疗对脊髓损伤患者近期及远期社会、心理、生理状况的影响.方法:回顾性分析2000年10月至2005年12月期间西安医学院第二附属医院和陕西中医药大学附属医院收治的180例2型脊髓损伤患者,分为观察组和对照组,各90例.对照组采用常规治疗,观察组在对照组治疗基础上采用康复治疗.比较两组患者近期及远期社会状态评分[脊髓损伤运动指数(AMS)、巴氏指数(BI)、功能独立性评定(FIM)]、心理状态评分[汉密顿抑郁量表(HAMD)、汉密顿焦虑量表(HAMA)]、生理状况指标[膀胱容量、最大尿流速率、残余尿量、脑源性神经生长因子(BDNF)和神经生长因子(NGF)].结果:近期及远期AMS、BI、FIM评分观察组显著高于对照组(均P＜0.05);HAMD、HAMA评分观察组显著低于对照组(均P＜0.05);观察组膀胱容量、最大尿流速率显著大于对照组,残余尿量显著少于对照组(均JP＜0.05).观察组血清BDNF和NGF水平显著高于对照组(均P＜0.05).结论:康复治疗可有效促进脊髓损伤患者近期及远期生理功能和社会、心理状态的恢复,提高其生活质量.

目的 探讨慢性硬膜下血肿(CSDH)钻孔引流术后脑膨胀不良的原因及治疗体会.方法 选择2015年1月至2018年5月于我院进行CSDH钻孔引流治疗后出现术后脑膨胀不良的34例患者为研究对象,出院后给予其阿托伐他汀治疗.结果 34例患者经不同疗程阿托伐他汀治疗后脑膨胀良好,硬膜下积液消失.结论 不同疗程的阿托伐他汀(20 mg/次,1次/d)辅助治疗CSDH钻孔引流术后脑膨胀不良效果显著,值得推广.