目的 探讨心理护理干预对颅内肿瘤患者围手术期负面情绪的影响.方法 选取2020年8月至2021年8月间西安医学院第二附属医院收治的50例颅内肿瘤患者,采用随机数表法分为研究组和对照组,每组25例.研究组患者采用心理护理干预,对照组患者采用常规护理干预,比较两组患者的护理满意度、负面情绪和生活质量.结果 研究组患者护理满意度为96.0％,高于对照组的76.0％,差异有统计学意义(P＜0.05).护理后,研究组患者的焦虑和抑郁评分均低于对照组,生活质量量表各项评分均高于对照组,差异均有统计学意义(均P＜0.05).结论 颅内肿瘤患者采用心理护理干预,可提升患者护理满意度,缓解负面情绪,改善生活质量,值得临床研究与借鉴.

目的 探讨优质护理对脑膜瘤切除术后并发症的预防效果.方法 选取2019年12月至2021年12月间西安医学院第二附属医院收治的90例脑膜瘤切除术患者,根据随机数字表法分为观察组和对照组,每组45例.对照组患者采用常规护理,观察组患者采用优质护理,比较两组患者的负性情绪、术后恢复指标、并发症总发生率和满意度.结果 护理后,观察组患者焦虑和抑郁评分均降低,且观察组低于对照组,差异均有统计学意义(均P<0.05).观察组患者肛门首次排气、首次下床时间和住院时间均短于对照组,差异均有统计学意义(均P<0.05).观察组患者并发症总发生率为2.2％,低于对照组的17.8％,差异有统计学意义(P<0.05).观察组患者护理满意度为95.6％,高于对照组的75.6％,差异有统计学意义(P<0.05).结论 优质护理可有效减轻脑膜瘤切除术患者的焦虑、抑郁等不良情绪,缩短术后机体恢复时间,降低并发症发生率,提高护理满意度.

目的 探讨围术期加速康复外科(ERAS)护理对脑胶质瘤患者并发症发生率的影响.方法 选取2018年10月至2020年10月间西安医学院附属第二医院收治的80例脑胶质瘤患者,采用随机数表法分为研究组和对照组,每组40例.研究组患者采用围术期ERAS护理干预,对照组患者采用常规康复护理干预,比较两组患者的护理满意度、并发症发生情况、焦虑抑郁情绪、疼痛评分、引流管拔除时间和住院时间.结果 研究组患者护理满意度为97.5％,对照组为77.5％,差异有统计学意义(P<0.05).研究组患者并发症发生率为5.0％,对照组为20.0％,差异有统计学意义(P<0.05).护理前,两组患者焦虑、抑郁、疼痛评分比较,差异无统计学意义(P>0.05);护理后,两组患者焦虑、抑郁、疼痛评分比较,差异均有统计学意义(均P<0.05).研究组患者引流管拔除时间和住院时间均短于对照组,差异均有统计学意义(均P<0.05).结论 脑胶质瘤患者采用围术期ERAS护理干预,可提升护理满意度,缓解焦虑、抑郁、疼痛症状,缩短引流管拔除时间和住院时间,减少术后并发症,建议临床借鉴.

Protein tyrosine phosphatase non-receptor type 21 (PTPN21) has been recognised as a new tumour-associated protein that is implicated in diverse tumours. However, the correlation between PTPN21 and glioma remains unaddressed. This investigation focused on the relevance of PTPN21 in glioma. The Cancer Genome Atlas (TCGA) analysis identified PTPN21 as being up-regulated in glioma tissue. The elevation of PTP21 in glioma was validated by evaluating clinical specimen. Kaplan-Meier plot analysis revealed that a high PTPN21 level predicted poor survival rate in glioma patient. Silencing of PTPN21 produced remarkable anticancer effects in glioma cells including proliferation inhibition, cell cycle arrest, metastasis suppression and enhanced chemosensitivity. Mechanistic studies uncovered that PTPN21 contributes to mediation of the phosphatidyl-inositole-3 kinase (PI3K)/AKT pathway via the regulation of epidermal growth factor receptor (EGFR). Restraint of EGFR diminished PTPN21 overexpression-induced promoting effect on PI3K/AKT pathway. Reactivation of AKT reversed PTPN21 silencing-evoked antitumor effect. The tumorigenic potential of PTPN21-silenced glioma cells in vivo was markedly compromised. In summary, this study demonstrates that silencing of PTPN21 produces remarkable anticancer effects in glioma by restraining the EGFR/PI3K/AKT pathway.

目的 探讨白藜芦醇对脑胶质瘤细胞增殖和侵袭的影响及可能的分子调控机制.方法 体外培养胶质瘤U251细胞,分别用浓度为0μmol/L、50μmol/L、100μmol/L、150μmol/L白藜芦醇继续培养48 h,参考Lipofectamine2000TM说明书将pcD?NA3.1/CD44(CD44过表达)、pcDNA3.1(过表达对照)等质粒转染胶质瘤U251细胞并培养24 h进行后续实验.利用CCK-8法检测细胞增殖,利用Transwell实验检测细胞侵袭;RT-PCR和免疫印迹法检测细胞CD44、CyclinD1、CDK4、MMP2和MMP9的mRNA和蛋白表达.结果 白藜芦醇明显抑制U251细胞的增殖和侵袭能力(P<0.05),而且呈浓度依赖性(P<0.05);所以,用150μmol/L白藜芦醇进行CD44干预实验.白藜芦醇明显抑制U251细胞CD44、CyclinD1、CDK4、MMP2和MMP9的mRNA和蛋白表达(P<0.05),而且呈浓度依赖性(P<0.05).CD44过表达明显抑制白藜芦醇对胶质瘤U251细胞增殖和侵袭的抑制作用(P<0.05.结论 白藜芦醇抑制胶质瘤细胞增殖和侵袭,其机制与下调CD44的表达有关.

目的 探讨对重症脑出血患者实施早期肠内营养(EEN)支持的临床效果.方法 选取2020年9月至2021年10月本院收治的300例重症脑出血患者为研究对象,采用随机排列法分为对照组和实验组,各150例.两组患者在入院12～48 h内均给予营养支持,其中对照组采用常规鼻饲饮食,实验组给予EEN干预.分别于入院第1 d和入院2周后比较患者营养状况和免疫功能的差异,观察患者预后情况.结果 两组干预前血清白蛋白(ALB)、前清蛋白(PA)、血清总蛋白(TP)三项营养状况指标水平和免疫球蛋白G(IgG)、免疫球蛋白A(IgA)和免疫球蛋白M(IgM)三项免疫功能指标水平比较差异无统计学意义(P＞0.05);干预后,对照组营养状况指标和免疫功能指标水平均低于本组干预前,差异有统计学意义(P＜0.05),而实验组营养状况指标和免疫功能指标水平均高于本组干预前,差异有统计学意义(P＜0.05);且实验组干预后ALB、PA、TP、IgG、IgA和IgM水平高于对照组,差异有统计学意义(P＜0.05).实验组并发症总发生率6％低于对照组25.33％,差异有统计学意义(P＜0.05).结论 EEN支持可以有效改善重症脑出血患者营养状况,提高患者的免疫功能,减少并发症的发生,为患者预后转归创造良好条件.

Isorhynchophylline (IRN), a component of traditional Chinese herb Uncaria rhynchophylla, possesses strong antioxidant activity. Ferroptosis induced by iron overload causes cell oxidative stress after intracerebral hemorrhage (ICH). Therefore, this study aims to explore the effects of IRN on the ferroptosis following ICH. In this study, mouse hippocampal HT-22 cells were treated with ferric ammonium citrate (FAC) alone or together with IRN, and we found IRN reduced the FAC-induced cell damage. Then, cells were treated with IRN following treatment with FAC after transfection with miR-122-5p inhibitor, and the results showed IRN reduced the FAC-induced decrease of miR-122-5p levels and relieved the ferroptosis by detecting ferroptotic marker proteins, iron ion concentration and oxidative stress level; after transfection with miR-122-5p inhibitor, the protective effects of IRN against FAC-induced ferroptosis in these cells were weakened. TP53 (also known as p53) was verified as a target of miR-122-5p by using dual luciferase reporter assay, and restoration of TP53 attenuated the effects of miR-122-5p on ferroptotic marker proteins expression, iron ion concentration and lipid ROS levels, as well as solute carrier family seven member 11 (SLC7A11) mRNA expression. SLC7A11 siRNA reversed the inhibitory effects of IRN on FAC-induced ferroptosis and oxidative stress levels. Subsequently, IRN increased the mNSS score, and decreased brain water content and EB content in ICH model. Moreover, IRN decreased ferroptosis and lipid ROS level, upregulated the expression of miR-122-5p and SLC7A11 mRNA, and inhibited TP53 expression. Our findings reveal that IRN protects neurocyte from ICH-induced ferroptosis via miR-122-5p/TP53/SLC7A11 pathway, which may provide a potential therapeutic mechanism for ICH.

目的:研究分析神经外科护理过程中,实施细节化干预的应用效果.方法:在2020年5月-2021年4月期间,随机选择西安医学院第二附属医院神经外科收治的200例住院患者为研究对象,根据入院单双号分为两组,研究组和参照组各100例.参照组实施神经外科常规护理,研究组在参照组的基础上进行细节化护理干预,比较分析两组的护理效果.结果:干预后,研究组的不良事件发生率(4.0％)明显低于参照组不良事件发生率(16.0％),两组比较差异具有统计学意义(P＜0.05).研究组的护理满意度(98.0％)高于参照组的护理满意度

Cognitive impairments are associated with advancing age. Trans-cinnamaldehyde (CIN) and ellagic acid (ELA) have multiplex activities to reduce various age-related cognitive disorders. In this study, we investigated the effects of these compounds separately or in combination on the cognitive outcomes, mitochondrial function, and inflammatory and apoptotic mediators in aged male Wistar rats. Thirty-two old (22 months old) and eight young (5 months old) rats were randomly allocated to five groups of young control, aged control, ELA-aged, CIN-aged, and ELA + CIN-aged. ELA (15 mg/kg, orally) and CIN (50 mg/kg, intraperitoneally) separately or in combination were administered for 1 month in aged animals. Spatial memory and cognitive activity were evaluated by the Barnes maze and novel object recognition tests. Mitochondrial function (its reactive oxygen species [ROS], mitochondrial membrane potential and ATP level), pro-inflammatory cytokines such as interleukin (IL)-1β and IL-6 and pro-apoptotic caspase 3 and Bax, and anti-apoptotic Bcl2 levels and their ratio were assessed in the prefrontal cortex. Behavioral results revealed that CIN separately or in combination with ELA significantly alleviates aging-induced memory impairment. Moreover, co-administration of agents effectively decreased inflammatory cytokines, cleaved-caspase 3, Bax and Bax/Bcl2 levels, mitochondrial ROS production, and mitochondrial membrane depolarization and increased Bcl2 and ATP level as compared with untreated aged control rats. Combination therapy was greater than those of individual treatments in all parameters. Therefore, combination therapy with CIN and ELA improved aging-induced cognitive impairment through anti-inflammatory, anti-apoptotic, and mitochondrial-boosting effects in aged rats.

目的 分析显微血管减压术中三叉神经根部浅表电凝治疗三叉神经痛的效果.方法 选取164例三叉神经痛患者随机分为两组,对照组给予常规显微血管减压术治疗,观察组给予显微血管减压术且术中对三叉神经根部浅表电凝.比较两组手术指标、不同时刻疼痛评分、临床疗效、术后1个月内并发症发生率及随访6个月复发率.结果 两组的手术时间、术中出血量相比差异均无统计学意义(P＞0.05),观察组住院时间短于对照组(P＜0.05);两组疼痛评分均随着时间的延长显著下降(P＜0.05),术后观察组的疼痛评分均低于对照组(P＜0.05);观察组总有效率高于对照组(P＜0.05);两组术后1个月内听力下降、脑脊液漏、面瘫的发生率及复发率相比差异均无统计学意义(P＞0.05),观察组面部麻木发生率高于对照组(P＜0.05).结论 显微血管减压术中三叉神经根部浅表电凝治疗三叉神经痛可以缩短住院时间,减轻疼痛感,提高临床疗效,虽然可增加面部麻木发生率,但可快速恢复.

目的:探讨康复治疗对脊髓损伤患者近期及远期社会、心理、生理状况的影响.方法:回顾性分析2000年10月至2005年12月期间西安医学院第二附属医院和陕西中医药大学附属医院收治的180例2型脊髓损伤患者,分为观察组和对照组,各90例.对照组采用常规治疗,观察组在对照组治疗基础上采用康复治疗.比较两组患者近期及远期社会状态评分[脊髓损伤运动指数(AMS)、巴氏指数(BI)、功能独立性评定(FIM)]、心理状态评分[汉密顿抑郁量表(HAMD)、汉密顿焦虑量表(HAMA)]、生理状况指标[膀胱容量、最大尿流速率、残余尿量、脑源性神经生长因子(BDNF)和神经生长因子(NGF)].结果:近期及远期AMS、BI、FIM评分观察组显著高于对照组(均P＜0.05);HAMD、HAMA评分观察组显著低于对照组(均P＜0.05);观察组膀胱容量、最大尿流速率显著大于对照组,残余尿量显著少于对照组(均JP＜0.05).观察组血清BDNF和NGF水平显著高于对照组(均P＜0.05).结论:康复治疗可有效促进脊髓损伤患者近期及远期生理功能和社会、心理状态的恢复,提高其生活质量.

目的 探讨重症颅脑损伤患者肺部感染的危险因素,并分析循证护理干预对策.方法 收集2018年2月至2019年2月该院神经外科收治的148例重症颅脑损伤患者的临床基础信息、病情评估、抗菌药物使用、不良事件发生情况及住院时间等资料,根据是否发生肺部感染将其分为感染组和对照组,进行统计学分析.结果 148例重症颅脑损伤患者中有33例(22.30％)发生肺部感染.感染组中年龄≥60岁、有吸烟史、营养不良、抗菌药物联用、发生不良事件者所占比例显著高于对照组,且呼吸机辅助通气时间、昏迷时间、气管切开或插管病程、住院时间明显长于对照组,差异均有统计学意义(P＜0.05),两组其余资料和指标比较,差异均无统计学意义(P＞0.05).Logistic回归分析显示,营养不良、抗菌药物联用、不良事件、呼吸机辅助通气时间、昏迷时间、气管切开或插管病程、住院时间为重症颅脑损伤患者发生肺部感染的危险因素(P＜0.05).结论 重症颅脑损伤患者肺部感染风险高,肺部感染与患者营养不良、抗菌药物应用、不良事件、病情及侵入性操作等诸多因素有关,应强化护理风险管理,尤其注重循证护理的应用.

目的 探讨慢性硬膜下血肿(CSDH)钻孔引流术后脑膨胀不良的原因及治疗体会.方法 选择2015年1月至2018年5月于我院进行CSDH钻孔引流治疗后出现术后脑膨胀不良的34例患者为研究对象,出院后给予其阿托伐他汀治疗.结果 34例患者经不同疗程阿托伐他汀治疗后脑膨胀良好,硬膜下积液消失.结论 不同疗程的阿托伐他汀(20 mg/次,1次/d)辅助治疗CSDH钻孔引流术后脑膨胀不良效果显著,值得推广.

目的 探讨循证护理模式对糖尿病高值(12～20)患者血糖水平及生存质量的影响.方法 选取我院糖尿病高值(12～20)患者110例,随机分为对照组与观察组,各55例,对照组行常规护理模式,研究组行循证护理模式,比较干预前后两组患者的血糖水平、生存质量评分变化以及护理满意率.结果 干预后两组患者各血糖指标水平均明显降低,且研究组低于对照组(P＜0.05),干预后两组患者在社会维度、生理维度、心理维度、治疗维度方面及生存质量总分均显著降低,且研究组低于对照组(P＜0.05),研究组患者并发症发生率低于对照组(P＜0.05),研究组患者护理满意率高于对照组(P＜0.05).结论 循证护理模式可有效控制血糖水平,提高患者的生存质量及护理满意率.

目的:通过有效的护理工作提高慢性硬膜下血肿患者的生活质量;方法:对患者围手术期,术前及术后不同的临床状态,采取不同的护理策略,并总结出"动静结合"的护理理念;结果:患者均恢复到了本次患病前的生活状态,在院期间无引流管脱落等护理不良事件发生;无肺部感染,下肢静脉血栓,泌尿系感染等并发症发生;结论:根据慢性硬膜下血肿患者治疗不同阶段的特点,采用不同的护理措施,并以"动静结合"的护理理念作为指导,能够有效减少并发症,并促进患者的心理康复,适合临床借鉴推广.

目的 探究CBL教学法联合PDCA循环在神经外科护理教学中的应用效果.方法 选择西安医学院护理专业2015级四年制护理专业本科生84例为研究对象,随机将其分为对照组和观察组,各42例.对照组采用传统教学法,试验组采用CBL教学法联合PDCA循环.比较两组理论知识、实践能力及综合素质,并分析该教学模式的整体评价结果 .结果试验组理论知识掌握情况、临床检查、病情评估、专科护理、应变能力、护患沟通、病历书写得分均高于对照组(P<0.05).试验组中超过95％的护生认为该教学模式有助于促进自主学习、独立思考及对理论知识的掌握应用,能提高分析解决问题、临床推理、应变、护患沟通能力及自信心等,90.48％的护生喜欢该教学模式.结论 CBL教学法联合PDCA循环能帮助护生提升综合素质及实践操作技能,且对今后工作中解决问题的方法和思维能够起到积极的作用.

PURPOSE:Accumulating evidence indicates that dysregulated long noncoding RNAs (lncRNAs) play critical roles in tumorigenesis and cancer progression. LncRNA-maternally expressed gene 3 (MEG3) has been shown to be involved in the initiation and development of several cancers, including glioma. However, the clinical prognostic value of MEG3 in glioma has not yet been fully elucidated.METHODS:The expression levels of MEG3 were detected in 79 glioma tissues and adjacent normal brain tissues, as well as, glioma cells and normal human astrocytes by qRT-PCR. Kaplan-Meier and Cox regression methods were utilized for the survival analysis. MTT assay, flow cytometry, and immunofluorescence assay were carried out to detect the impact of MEG3 on glioma cell proliferation, apoptosis, and autophagy.RESULT:The current results showed that MEG3 expression was significantly downregulated in glioma tissues and cell line and negatively correlated with WHO grade in glioma patients. Low MEG3 expression was significantly associated with the advanced WHO grade, low Karnofsky performance score (KPS), IDH wild-type, and tumor recurrence. Patients displaying a low expression of MEG3 contributed to poor overall survival. The downregulated level of MEG3, advanced WHO grade, low KPS, IDH wild-type, and tumor recurrence were independent poor prognostic indicators in glioma patients. The in vitro experiments demonstrated that the MEG3 overexpression remarkably suppressed the proliferation while facilitating apoptosis and autophagy in glioma cells.CONCLUSIONS:These findings indicated a critical role of MEG3 in glioma cell proliferation, apoptosis, and autophagy. Also, the gene was found to be significantly associated with the prognosis in glioma patients. Thus, it might provide a new target for predicting prognosis and therapeutic intervention in glioma.

目的：分析胃癌患者术后早期肠内营养护理心得。方法：将2012年11月－2014年11月期间收治于我院的胃癌术后早期肠内营养支持患者60例为研究对象，随机将其均分成观察组和对照组，每组患者各30例。其中给予对照组患者常规护理；给予观察组患者优质护理；对比分析两组患者的排气时间、进食时间、排便时间、住院时间等。结果：在经过不同的护理模式后，观察组患者无论是排气时间、进食时间、排便时间、住院时间均短于对照组（P ＜0．05），两组间差异显著。结论：针对于胃癌术后早期肠内营养支持治疗患者来讲，对其进行有针对性的优质护理措施能够改善患者的预后情况，缩短排气时间、进食时间、排便时间以及住院时间，临床中值得推广使用。

Objective:To explore the effect of nursing intervention after breast cancer operation on the affected limb function exercise rehabilitation.Methods:112 patients with breast cancer were randomly divided into the experimental group and the control group.The control group were given conventional nursing,and the experimental group were given nursing intervention based on routine nursing mode.We compared the functional recovery condition of the patients in two groups.Results:In the experimental group,the basic function recovery was significantly better than that of the control group(P<0.05).Conclusion:Nursing intervention can effectively improve the curative effect of limb function recovery of patients after breast cancer operation, and enhance the effect of functional exercise.

<正>介入治疗是中晚期肿瘤的重要治疗手段,可以有效缩小肿瘤体积,减缓其生长,从而延长患者的生存时间,提高患者生活质量。但是,恶性肿瘤患者本身往往存在焦虑、抑郁和悲观等不良情绪,加上对治疗的恐惧,给患者的治疗和生活均造成不良影响。我们对107例行介入治疗的肝癌患者进