选择80只36周龄产蛋种母鸡,按产蛋率相近的原则随机分成4组,分别为对照组(试验1组)和受试药推荐剂量1、3、5倍组(试验2、3、4组),分别按博普总碱散0、400、1200、2000mg· kg-1(饲料)的剂量混饲给予,连用10d,通过观测给药前、给药9～10 d和休药5～6 d的母鸡产蛋率、种蛋品质及受精率、孵化率和出壳苗鸡健康状况、体重等指标,考察博普总碱散对种鸡及其种蛋是否存在生殖毒性.结果 表明:与对照组相比,给予受试药物1、3、5倍剂量的3个试验组种鸡产蛋率、种蛋重、蛋壳强度、蛋白高度、蛋黄颜色及哈氏单位等蛋品质指标均无显著差异;与给药前和对照组种蛋受精率及孵化率相比,3个试验组在给药后9～10 d及休药5～6 d种蛋的受精率和孵化率有显著提高.这一研究提示博普总碱散对种鸡及种蛋的繁殖性能具有较好的安全性,且能提高种蛋的受精率和孵化率.

选取12~20日龄感染流行性腹泻病毒腹泻猪,采用复方中药和西药抗生素进行临床治疗,治疗结束后取胃底部、十二指肠中段、空肠中段、回肠中段和结肠中段,通过场发射扫描电镜和透射电镜对比观察消化道病变;取回肠,通过免疫组化法检测琥珀酸脱氢酶、单胺氧化酶、5′-核苷酸酶和Na+-K+-ATP酶活性变化.场发射扫描电镜观察结果表明,中药治疗猪未见异常,西药治疗猪十二指肠黏膜表面见少量黏附的大肠杆菌.透射电镜观察结果表明,中药治疗猪细胞结构接近正常,无严重细胞损伤,但细胞微丝管、骨架等结构不如健康猪清晰;西药治疗猪消化道可见内质网损伤,内质网和高尔基体断裂,核形态和核染色质异常.琥珀酸脱氢酶、单胺氧化酶和Na+-K+-ATP酶检测结果均为中药治疗猪酶活性显著高于西药治疗猪.由结果可以推测,复方中药治疗猪流行性腹泻的良好效果可能与保护线粒体、稳定细胞结构、稳定膜结构、调整酶活性和抗继发感染有关.

A total of 56 12-20 day-old pigs which infected by porcine epidemic diarrhea virus were treated with Chinese medicine and western medicine antibiotic.The therapeutic effect was evaluated by the observation of cure time,body weight changes,cure rate and recurrence rate.The therapeutic effect was further determined and the mechanism was explored by paraffin sections of stomach,duodenum,jejunum,ileum and colon of pigs treated with Chinese medicine and western medicine antibiotics.Serum levels changes of TGF-α,TGF-β,IgM,IgA,IgG,IL-1 and IL-4 were compared after treatment with the untreated results.Clinical treatment result:Chinese medicine treatment group in an average of 4.42 days to cure,86.67％ cure rate,0.00％ recurrence rate,0.59％ maximum weight decreased,22.44％ weight increased at 11 days,thewestern medicine antibiotics treatment group in an average of 4.91 days to cure,84.61％ cure rate,7.69％ recurrence rate,5.08％ maximum weight decreased,compared with the traditional Chinese medicine group the difference was significant(P＜0.01),20.94％ weight increased at 11 days.Paraffin section results:The pig of Chinese medicine treatment was basically returned to normal after treated.Inflammato ry response was obvious while pig after western medicine antibiotics treated,and the progress of mucosal repaired was slow.Serum index results:IgM of Chinese medicine treatment group and western medicine antibiotics treatment group was significantly higher than treated before,IL-1 of Chinese medicine treatment group and western medicine antibiotics treatment group was significantly lower than treated before,IL-4 of western medicine antibiotics treatment group was significantly higher than treated before.Chinese medicine is effective in the treatment of porcine epidemic diarrhea,it can accelerate the tissue repair,reduce the inflammatory reaction,protect the mitochondria,stabilize the cell structure and regulate the immunity.

为了观察苦参止痢颗粒对犬细小病毒病的治疗作用,试验以水平感染的犬作为动物模型,将其分为中药组、西药组、阳性组和空白组,用苦参止痢颗粒和西药进行治疗,观察临床症状、病理解剖和组织病理学切片.结果表明:苦参止痢颗粒和西药都能控制犬细小病毒病,其中中药组没有出现血痢症状,组织病理学切片观察肠道组织也没有明显出血,并且维持了肠绒毛结构的完整性.说明苦参止痢颗粒能抑制犬细小病毒引起的肠黏膜损伤和出血性病变,对犬细小病毒病可达到治愈的作用.