Primary open-angle glaucoma (POAG) is now seen as a progressively worsening neurodegenerative disorder. Previvors neuroimaging studies neglect dynamic brain activity, assuming static patterns in resting-state functional MRI (rs-fMRI), while intrinsic brain activity is dynamic and fluctuates over time. To test the time-varying brain activity in POAG patients, a temporal dynamic analysis of rs-fMRI data was conducted on 70 POAG patients and 45 healthy controls (HCs). The sliding-window method calculated dynamic amplitude of low-frequency fluctuations (dALFF) across the brain, and group differences and correlations with ophthalmological and neuropsychological measures were analyzed. Multivariate pattern analysis evaluated dALFF and static-ALFF's ability to differentiate POAG patients from HCs. POAG patients exhibited lower dALFF in visual network areas and higher dALFF in the default mode, ventral attention, and frontoparietal networks compared to HCs. Decreased dALFF in the cuneus correlated with visual field deficits. Visual network dALFF distinguished POAG patients from HCs with 79.13 % accuracy, 66.67 % sensitivity, and 87.14 % specificity (p = 0.001). The dALFF's area under the curve was significantly greater than that of static-ALFF (p = 0.026). POAG patients show irregular time-varying brain activity, especially in areas linked to vision, movement, and emotional/cognitive functions. This study suggests that dALFF offers a novel approach to capture the aberrant time-varying brain activity and its clinical relation, holding potential for enhancing the understanding of POAG mechanisms and improving its diagnosis.

PRÉCIS:Hypobaric hypoxia, the major environmental factor at high altitudes, has been observed to induce pupil miosis and widening of the anterior chamber angle. This environment may be safe for individuals with narrow angle and deserves further study. PURPOSE:This study aimed to quantify anterior chamber biometric parameters before and after acute short-term, effortless exposure to hypobaric hypoxia (HH) in healthy lowlanders using swept-source anterior segment optical coherence tomography (SS AS-OCT). METHODS:This prospective study included 25 healthy young lowlanders (50 eyes) who underwent SS AS-OCT measurements and intraocular pressure (IOP) assessments under baseline sea-level conditions (T1). They were then passively exposed to simulated 4000 m above sea level for 3 hours and underwent acute mountain sickness (AMS) symptoms evaluation and IOP measurement after 2 hours exposure to HH (T2). Repeat SS AS-OCT measurements and IOP assessments were taken within 15 minutes after leaving the hypobaric chamber (T3). Anterior segment parameters including anterior chamber depth (ACD), lens vault (LV), angle opening distance (AOD500), trabecular-iris space area (TISA500), angle recess area (ARA500) at 500 μm from the scleral spur, iris curvature (IC), iris volume (IV), pupil diameter (PD), and central corneal thickness (CCT) were obtained through SS AS-OCT. These repeated measurements were compared using linear mixed model analysis. RESULTS:In comparison to the sea level, both IOP (16.4±3.4 vs. 14.9±2.4 mm Hg, P =0.029) and PD (5.36±0.77 vs. 4.78±0.89 mm, P =0.001) significantly decreased after exposure to HH. Significant post-HH changes [mean difference (95% CI)] were observed in AOD500 [0.129 (0.006, 0.252), P =0.04], TISA500 [0.059 (0.008, 0.11), P =0.025], ARA500 [0.074 (0.008, 0.141), P =0.029], IV [1.623 (0.092, 3.154), P =0.038], and IC [-0.073 (-0.146, 0.001), P =0.047], whereas CCT, ACD, and LV remained stable. After adjusting for age, post-HH variations in AOD500 (Beta=0.553, 95% CI: 0.001, 1.105, P =0.048) and TISA500 (Beta=0.256, 95% CI: 0.02, 0.492, P =0.034) were associated with decreased IC but were not related to lowered arterial oxygen pressure or IV increase per millimeter of pupil miosis (IV/PD). These differences in anterior segment parameters were neither correlated with differences in IOP nor AMS. CONCLUSIONS:After short-term, effortless exposure to hypobaric hypoxia, pupil miosis occurred with widening of the anterior chamber angle and decreased IC. These changes in anterior chamber angle parameters were associated with decreased IC but did not correlate with the post-hypobaric variations in IV/PD, IOP, or AMS.

To assess the long-term outcomes of patients with glaucoma secondary to iridocorneal endothelial syndrome (GS-ICE) who underwent Ahmed glaucoma valve (AGV) implantation combined with phacoemulsification and intraocular lens (IOL) implantation surgery. In this non-comparative retrospective case series study, twelve patients with uncontrolled GS-ICE and cataract underwent Ahmed aqueous shunt combined with phacoemulsification and IOL implantation surgery at Beijing Tongren Eye Center between June 2014 and June 2022. Main medical records included best-corrected visual acuity (BCVA), intraocular pressure (IOP), number of antiglaucoma medications (AGM), corneal status and further surgical interventions. Surgical success was defined as a postoperative IOP ≥ 6mmHg and ≤ 21mmHg without (complete success) or with/without (qualified success) topical AGM and vision of at least light perception during the follow-up period. Twelve eyes of twelve patients were reviewed. Preoperative IOP was decreased from a mean of 38.5 ± 6.7 mmHg on 3.3 ± 0.9 AGM to a mean of 16.3 ± 4.3 mmHg (P<0.001) on 0.6 ± 1.1 medications (P<0.001) at the last follow-up (47.0 ± 24.7 months). Eight eyes (66.7

To compare the corneal biomechanical parameters measured by Corvis ST in subjects with varying degrees of myopia. And the factors that may affect corneal biomechanical properties were also investigated. Participants in this prospective cross-sectional study were classified into three groups according to spherical equivalent (SE) and axial length (AL): Non-myopia (NM, SE > - 0.50 D and AL < 26 mm), Mild-to-moderate myopia (MM, - 6.00 D < SE ≤ - 0.50 D and AL < 26 mm), high myopia (HM, SE ≤ - 6.00 D or AL ≥ 26 mm). Ten corneal biomechanical parameters were finally included. Linear mixed-effects model accounting for using both eyes in the same participant was carried out to evaluate how the corneal biomechanical parameter was influenced by varying degrees of myopia after adjusting for biomechanically corrected intraocular pressure (bIOP) and central corneal thickness (CCT). Further, multiple linear regression was performed to explore the correlation between corneal biomechanical parameter and SE, AL, bIOP or CCT. A total of 304 eyes from 224 healthy myopic subjects were recorded. There were 95 eyes with NM, 122 eyes with MM, and 87 eyes with HM. After adjusting for bIOP and CCT, eyes with high myopia showed shorter highest concavity time (HC-time, p = 0.025), greater peak distance (PD, p = 0.001), greater deflection amplitude (DA-Max, p = 0.002), smaller whole eye movement (WEM-Max, p < 0.001) and reduced stiffness parameter (SP-A1, p < 0.001). Multiple regression analysis showed that five parameters (HC-time, p < 0.001; PD, p < 0.001; DA-Max, p = 0.001; WEM-Max, p < 0.001; and SP-A1, p < 0.001) of Corvis ST were significantly correlated with AL, and one parameter (Corvis biomechanical index, p = 0.016) has significant relationship with SE. With the increase of myopia, significant changes in several corneal biomechanical parameters indicated a progressive decrease in corneal stiffness, independent of bIOP and CCT. Corneal biomechanical parameters may be predictors of scleral mechanical strength in high myopia, which has certain application value in clinical management of myopia.

AIMS:To evaluate the association between contrast sensitivity function (CSF) and glaucomatous structural damage in primary open-angle glaucoma (POAG). METHODS:A cross-sectional study was performed with 103 patients (103 eyes) aged 25-50 years who had POAG without any other ocular disease. CSF measurements were obtained by the quick CSF method, a novel active learning algorithm that covers 19 spatial frequencies and 128 contrast levels. The peripapillary retinal nerve fibre layer (pRNFL), macular ganglion cell complex (mGCC), radial peripapillary capillary (RPC) and macular vasculature were measured by optical coherence tomography and angiography. Correlation and regression analyses were used to assess the association of area under log CSF (AULCSF), CSF acuity and contrast sensitivities at multiple spatial frequencies with structural parameters. RESULTS:AULCSF and CSF acuity were positively associated with pRNFL thickness, RPC density, mGCC thickness and superficial macular vessel density (p<0.05). Those parameters were also significantly associated with contrast sensitivity at 1, 1.5, 3, 6, 12, 18 cycles per degree spatial frequencies (p<0.05) and, the lower the spatial frequency, the higher the correlation coefficient. RPC density (p=0.035, p=0.023) and mGCC thickness (p=0.002, p=0.011) had significant predictive value for contrast sensitivity at 1 and 1.5 cycles per degree, with adjusted R 2 of 0.346 and 0.343, respectively. CONCLUSIONS:Full spatial frequency contrast sensitivity impairment, most notably at low spatial frequencies, is a characteristic change in POAG. Contrast sensitivity is a potential functional endpoint for the measurement of glaucoma severity.

BACKGROUND:Blood perfusion of the optic nerve (ON) plays a key role in many optic neuropathies. Microvascular changes precede or accompany neuronal changes, and detecting these changes at an early stage may facilitate early treatment to avoid blindness. However, the quantification of ON blood perfusion remains a challenge. This study aimed to evaluate the viability of three-dimensional pseudocontinuous arterial spin labelling (3D-pCASL) MRI for the quantification of ON blood flow (BF). NEW METHOD:The ON segmentation was performed using nnFormer on a cohort of ten participants (4 males, 6 females, 25-59 years old). Subsequently, the mean BF of each ON segment was calculated using whole brain 3D-pCASL image data. RESULTS:The average ON-BF values of the left and right intraorbital segments, left and right intracanalicular segments, left and right intracranial segments, optic chiasma, and left and right optic tract were 41.308 mL/100 g/min, 43.281 mL/100 g/min, 53.188 mL/100 g/min, 57.202 mL/100 g/min, 45.089 mL/100 g/min, 49.554 mL/100 g/min, 42. 326 mL/100 g/min, 43.831 mL/100 g/min and 45.176 mL/100 g/min, respectively. The ON-BF correlated with cerebral BF (r = 0.503, p = 0.024). COMPARISON WITH EXISTING METHOD(S):The 3D-pCASL can measure tissue microvascular blood perfusion in absolute quantitative units with good test-retest repeatability over a wide field of view and without restrictions on depth. The use of the nnFormer makes the measurement easy, objective and reproducible. CONCLUSIONS:The study showed that, 3D-pCASL may be a promising tool for detecting abnormal ON-BF. In particular, 3D-pCASL coupled with the nnFormer provides an objective, reproducible, and reliable method to quantify BF in ON.

Purpose Primary open-angle glaucoma (POAG) is a widespread neurodegenerative condition affecting brain regions involved in visual processing, somatosensory processing, motor control, emotional regulation and cognitive functions. Cerebral hemodynamic dysfunction contributes to the pathogenesis of glaucomatous neurodegeneration. We aimed to investigate cerebral blood flow (CBF) redistributed patterns in visual and higher-order cognitive cortices and its relationship with clinical parameters in POAG, and we hypothesized that CBF changes together across regions within the same functional network. Methods Forty-five POAG patients and 23 normal controls underwent three-dimensional pseudocontinuous arterial spin labeling MRI to measure the resting-state CBF. Group comparisons of CBF and correlations between CBF changes and ophthalmological and neuropsychological indices were assessed. We determined CBF-based functional connectivity (CBFC) by calculating the correlations between specific regions and all other brain voxels and compared CBFC differences between groups. Results The patients exhibited decreased CBF in visual cortices, postcentral gyrus, inferior parietal lobule and cerebellum and increased CBF in medial, middle, and superior frontal gyri, as well as the insula. The reduced CBF in the visual cortices positively correlated with visual field defect ( r = 0.498, p = 0.001) in POAG patients, while the increased CBF in the right medial frontal gyrus was negatively associated with the visual field defect ( r = −0.438, p = 0.004) and positively associated with the cup-to-disc ratio ( r = 0.469, p = 0.002). POAG patients showed negative connections weakening or converting to mild positive connections, as well as positive connections converting to negative connections. Conclusions Regional and interregional CBF properties confirmed that the aberrant brain regions extend beyond the visual pathway, including the somatosensory, emotional and cognitive networks, which highlights the importance of cerebral hemodynamic dysfunction in the pathophysiology of spreading neurodegeneration in POAG.

Increased cerebral blood flow resulting from altered capillary level autoregulation at high altitudes leads to capillary overperfusion and then vasogenic cerebral edema, which is the leading hypothesis of acute mountain sickness (AMS). However, studies on cerebral blood flow in AMS have been mostly restricted to gross cerebrovascular endpoints as opposed to the microvasculature. This study aimed to investigate ocular microcirculation alterations, the only visualized capillaries in the central neural system (CNS), during early-stage AMS using a hypobaric chamber. This study found that after high altitude simulation, the optic nerve showed retinal nerve fiber layer thickening (P=0.004–0.018) in some locations, and the area of the optic nerve subarachnoid space (P=0.004) enlarged. Optical coherence tomography angiography (OCTA) showed increased retinal radial peripapillary capillary (RPC) flow density (P=0.003–0.046), particularly on the nasal side of the nerve. The AMS-positive group had the largest increases in RPC flow density in the nasal sector (AMS-positive, Δ3.21±2.37; AMS-negative, Δ0.01±2.16, P=0.004). Among multiple ocular changes, OCTA increase in RPC flow density was associated with simulated early-stage AMS symptoms (beta=0.222, 95

Delineating the neuropathological characteristics of primary open-angle glaucoma (POAG) is critical for understanding its pathophysiology. While temporal stability represents a crucial aspect of the brain's functional architecture, the specific patterns underlying its contribution to POAG remain unclear. This study aims to analyze the brain functional abnormalities in POAG using functional stability, a dynamic functional connectivity (DFC) approach based on resting-state functional magnetic resonance imaging (rs-fMRI). Seventy patients with POAG and forty-five healthy controls underwent rs-fMRI and ophthalmological examinations. The stability of DFC was calculated as the concordance of DFC over time using a sliding-window approach, and the differences in stability between the two groups were compared. Subsequently, Spearman’s correlation analyses were conducted to examine the relationship between functional stability and clinical indicators. Compared with healthy controls, patients with POAG exhibited significantly decreased functional stability in the visual network, including the early visual center, ventral and dorsal stream visual cortex in both hemispheres. Conversely, stability values increased in the bilateral inferior parietal gyrus and right inferior frontal cortex. In POAG patients, the dynamic stability of the left early visual cortex and ventral stream visual cortex correlated with the mean deviation of visual field defects ( r = 0.251, p = 0.037). The evidence from this study suggests that functional stability may provide a new understanding of brain alterations in the progression of POAG.

This study aimed to illustrate the efficacy of the combination of lens extraction, trabeculectomy, and anterior vitrectomy in patients with secondary angle-closure glaucoma (ACG) with autosomal recessive bestrophinopathy or Best vitelliform macular dystrophy. This is a retrospective self-controlled case series study. Five patients undergoing a single trabeculectomy in one eye and triple surgery in the other eye were enrolled. All patients underwent a complete ophthalmic examination that included best-corrected visual acuity (BCVA), intraocular pressure (IOP), ultrasound biomicroscopy, and static gonioscopy. Multimodal fundus imaging was performed, including color fundus photography, fundus autofluorescence, and optical coherence tomography. Genetic testing was also analyzed. Among the 10 eyes, the mean IOP was 31.4 ± 4.7 mmHg before surgery. The mean axial length (AL) was 21.53 mm and the anterior chamber depth (ACD) was 2.31 mm. There were no statistically significant differences in preoperative IOP, BCVA, ACD, and AL between the two groups (all P > 0.05). The mean follow-up time was 64.0 months. All five eyes with a single trabeculectomy developed malignant glaucoma (MG). No complications were found in the other five eyes with triple surgery, and the anterior chamber was deepened and stable after surgery until the last visit. The mean IOP at the last visit was normalized to 16 mmHg without using any medications. Triple surgery is superior to single trabeculectomy for patients with ACG and BEST1 mutation, effectively bypassing MG complications. The vitreous may play a vital role in the mechanism of ACG in those patients and the high incidence of MG after filtering surgery.

目的 分析开角型青光眼（open-angle glaucoma,OAG）在多层级青光眼门诊中疾病亚型分布情况及人口学特点。设计回顾性病例系列。研究对象青光眼专科门诊586例OAG患者。方法 回顾2018年9月至2020年2月北京同仁医院（主要眼科诊疗中心）、成都市中西医结合医院（次级眼科诊疗机构A类）、邯郸市眼科医院（次级眼科诊疗机构B类）青光眼门诊患者资料。分析OAG患者疾病亚型、性别、年龄、患者来源地。主要指标患者人口学特征及OAG亚型构成比。结果 诊疗9584人次中,纳入OAG患者586例。平均年龄（52.35±16.48）岁,男性占59.7%。各OAG亚型中原发性开角型青光眼（primary open-angle glaucoma,POAG）429例（73.2%）,平均年龄（51.74±16.61）岁,男性占61.8%;正常眼压青光眼（normal-tension glaucoma,NTG）108例（18.4%）,平均年龄（54.97±15.31）岁,男女均为50.0%;继发性OAG 49例（8.4%）。与主要眼科诊疗中心比,次级眼科诊疗机构NTG比例更低（分别为23.0%、10.6%,χ~2=14.06,P＜0.01）,继发性OAG比例较高（分别为5.1%、13.8%,χ~2=13.42,P＜0.01）。主要眼科诊疗中心外省市患者占73.2%,远高于次级眼科诊疗机构（A类9.2%,χ~2=110.58,P＜0.01;B类3.5%,χ~2=96.97,P＜0.01）。结论 青光眼专科门诊中OAG患者性别、年龄分布与既往人群研究结果相似,但NTG患者比例较人群研究结果低。主要眼科诊疗中心青光眼门诊中外省市患者比例高,NTG患者较多,继发性OAG患者较少。（眼科,2022,31:8-13）

This study aimed to provide a 24-month follow-up on the surgical success and safety of gonioscopy-assisted transluminal trabeculotomy (GATT) combined with phacoemulsification and intraocular lens (IOL) implantation in the treatment of patients with primary open-angle glaucoma (POAG) combined cataract.We included 124 consecutive cases of POAG with microcatheter-assisted GATT or GATT combined with phacoemulsification and IOL implantation at Beijing Tongren Eye Centre between October 2019 and November 2020. Main outcome measures included surgical success rate, changes in IOP, number of antiglaucoma medications, best corrected visual acuity (BCVA), postoperative complications at baseline, and follow-up period of up to 24 months.In total, 58 eyes received GATT combined with phacoemulsification surgery and 66 eyes received GATT alone. The overall qualified success rate was 86.21% for eyes with GATT combined with phacoemulsification surgery, and 83.48% for eyes with GATT only at 24 months. IOP was reduced from 26.40 ± 6.37 mmHg on 3.12 ± 0.80 medications preoperatively to 14.61 ± 2.28 mmHg on 0.27 ± 0.71 medications at 12 months and 16.08 ± 2.38 mmHg on 0.45 ± 0.96 medications at 24 months after combined surgery. Additionally, mean BCVA improved from 0.75 ± 0.43 logMAR units preoperatively to 0.22 ± 0.18 logMAR units 24 months after combined surgery. No vision-threatening complications occurred during the 24-month follow-up.The 24-month follow-up results of our study suggest that GATT combined with cataract surgery is a safe and effective treatment for decreasing IOP and number of medications in patients with POAG combined cataract.

Objective:To compare performance assessment of two methods of measuring radial peripapillary capillary (RPC) vessel density (VD) after skeletonization using MATLAB and Image J in glaucoma clinical setting. Methods:Seventy-three eyes of 73 glaucoma patients from Beijing Tongren Hospital were included in this prospective study. Original images of RPC were obtained using optical coherence tomography angiography. Two approaches were executed before measuring. Method 1 (M1) required image sharpening, removal of big vessels, and skeletonization. Method 2 (M2) required skeletonization and removal of major vessels. Each method was executed twice. Repeatability and correlations with glaucomatous parameters were assessed. Factors associated with retinal nerve fiber layer thickness (RNFLT) and visual field mean deviation (MD) were analyzed. Results:Average VD was 13.86 ± 2.73 and 7.50 ± 2.50% measured by M1 and M2. Percentage of total elimination of the major vessels was 36.99 and 100% by M1 and M2, respectively. The intrasession and intersession reproducibility was higher by M2 (ICC = 0.979, ICC = 0.990) than by M1 (ICC = 0.930, ICC = 0.934). VD measured by M2 showed stronger correlations with glaucomatous parameters than by M1. By stepwise multiple linear regression, thinner RNFLT was associated with smaller VD measured by M2 (B = 4.643, P < 0.001). Worse MD was associated with smaller VD measured by M1 (B = 1.079, P = 0.015). Conclusion:The VD measured by M2 showed better reproducibility and higher correlation with glaucomatous structural parameters. Image sharpning helps display of hazy vasculature in glaucoma, which may reflect visual function better. Researchers should carefully choose image processing methods according to their research object.

PURPOSE:To investigate the changes in choroidal thickness (CT) after acute cerebrospinal fluid pressure (CSFP) reduction in human subjects. METHODS:Before and 15 minutes after diagnostic lumbar puncture (LP), 44 patients underwent measurement of CT by swept-source optical coherence tomography. Thirty-two healthy volunteers imitated the body posture of LP procedure and underwent the same measurement before and 15 minutes after body posture change. RESULTS:After CSFP reduction from 10.9 ± 2.1 mmHg at baseline to 8.1 ± 1.5 mmHg (p < 0.001), CT decreased in subfoveal region (p = 0.005), small to medium vessel layer (SMVL, p < 0.001), peripapillary regions in temporal (p = 0.001), nasal (p < 0.001), superior (p < 0.001) and inferior (p < 0.001), respectively. However, no significant change in CT in the control group after body posture change (all p > 0.05). A significant association between CSFP and the ratio of small to medium vessel layer to total choroidal thickness was found (p = 0.009). The CSFP reduction rate was associated with the change rate of SMVL to total CT portion, for each percent decrease in CSFP was associated with a decrease by 0.22% in the rate of SMVL to total CT portion (R2 = 0.125, p = 0.018). CONCLUSIONS:A significant decrease in subfoveal CT, small to medium vessel layer and peripapillary region were observed following acute CSFP reduction. The CSFP reduction rate was associated with the change rate of small to medium vessel layer to total CT portion.

The present study aims to investigate the effect of temporary cerebrospinal fluid pressure（CSFP） reduction on optic nerve head（ONH） and macular vessel density（VD） using optical coherence tomography angiography.Forty-four eyes of 44 adults with diagnostic lumbar puncture and CSFP reduction were recruited.Thirty-two eyes of 32 healthy volunteers were controls.ONH and macular VD images were evaluated differences between baseline and after CSFP reduction.The results showed that the mean CSFP decreased from（11.6±2.1） mm Hg to（8.2±3.4） mm Hg（P<0.001）.VD in the macular regions decreased significantly after CSFP reduction in the study group（all P<0.05）.The control group showed no significant changes in macular VD（all P>0.05）.In the study group,decreased VD in the macular parainferior region was associated with CSFP reduction（R 2 =0.192,P=0.003）,the reduction of macular VD in parafoveal（R 2 =0.098,P=0.018）,parainferior（R 2 =0.104,P=0.021）,parasuperior（R 2 =0.059,P=0.058）,paranasal（R 2 =0.057,P=0.042）,paratemporal（R 2 =0.079,P=0.026） was associated with mean ocular perfusion pressure decrease following CSFP reduction.ONH vessel density did not differ after CSFP reduction（all P>0.05）.In conclusion,macular vessel density decreased in association with CSFP reduction.Retinal vessel density in the macular region is more sensitive than that in peripapillary region after CSFP reduction.

Objective: To determine whether systemic vascular dysregulation (SVD) evaluated by nailfold capillaroscope and Flammer Syndrome Questionnaire (FSQ) affects retinal peripapillary microcirculation in non-glaucomatous healthy eyes at steady status. Methods: 120 healthy eyes from 63 non-glaucomatous subjects underwent Optical coherence tomography angiography (OCTA) after a rest of 30 minutes. Average retinal peripapillary capillary (RPC) vessel density (VD) and sectoral VD were automatically calculated, and peripapillary retinal nerve fiber layer thickness (RNFLT) was measured. Vasospastic diathesis was assessed using Flammer Syndrome Questionnaire (FSQ). Cold provocation test (CPT) was performed using nail-fold capillaroscope after OCTA. Positive CPT and a score of FSQ higher than 20% were necessary to determine a subject with SVD. Systemic and ocular parameters were compared between subjects with and without SVD. Results: In this study, heart rate ( p = 0.042), ocular perfusion pressure ( p = 0.014) and average RPC vessel density ( p = 0.046) was significantly different between subjects with and without SVD determined by the combination of CPT and FSQ. Generalized estimation equation (GEE) showed lower VD was significantly associated with longer axial length (β = −0.352, p = 0.001), thinner peripapillary retinal nerve fiber layer thickness (RNFLT) (β = 0.296, p < 0.001), SVD determined by CPT and FSQ (β = 0.617, p = 0.003) and high blood pressure (β = −0.879, p < 0.001). In the superotemporal sector, multivariate model showed only SVD was associated with RPC vessel density (β = −0.811, p < 0.001). Conclusion: In subjects with SVD and non-glaucomatous healthy eyes, lower RPC vessel density in the superotemporal sector was observed. SVD determined by CPT and FSQ was significantly associated with attenuated retinal peripapillary microcirculation. Studies on ocular diseases relevant to vasospasms should consider the effects of SVD on the retinal peripapillary capillaries.

Objectives To evaluate the association between serum retinol concentration and normal-tension glaucoma (NTG). Methods A total of 345 study subjects were recruited in a prospective cross-sectional study: 101 patients with NTG, 106 patients with high-pressure primary open-angle glaucoma (POAG) and 138 healthy control subjects. Serum retinol concentration in fasting blood samples was determined by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). All study subjects were given complete ophthalmic examinations and diagnosed by two glaucoma sub-specialists. Results Serum retinol concentrations in NTG, POAG, and controls were 338.90 ± 103.23 ng/mL, 405.22 ± 114.12 ng/mL, and 408.84 ± 122.36 ng/mL respectively. NTG patients had lower serum retinol concentrations than POAG ( p < 0.001) or healthy controls ( p < 0.001). There was no statistical difference between the POAG and healthy controls ( p = 0.780). Higher proportion of NTG patients (37.6%) than POAG (17.9%) or controls (21.7%) had serum retinol concentrations lower than 300 ng/mL. Serum retinol was positively correlated with optic nerve sheath diameter (ONSD) ( r = 0.349, p = 0.001) in glaucoma patients and not associated with any other demographic features or ophthalmic biometric parameters in the NTG patients. Multivariate logistic regression showed that serum retinol (OR = 0.898, 95CI%: 0.851–0.947) was associated with incident NTG. Conclusions NTG patients had lower serum retinol concentrations. Serum retinol uniquely associated with NTG makes it a new potential option for the diagnosis and treatment of the disease.

王宁利教授是北京学者,入选国家高层次人才（万人计划）、国际眼科科学院院士。长期致力于全球首位不可逆致盲眼病青光眼的防治研究,领导并承担多项国内外重大防盲工程和任务,在大家的共同努力下,使我国眼病总致盲率降低了27%,并通过加强国际合作分享中国的成果。

The optic nerve is the only pair of cranial nerves that pass through three sealing containers with pressure. First, the retinal ganglion cell bodies and its gathering of the axons belong to the ocular container. Then, the rest part of the axons is surrounded by cerebrospinal fluid along its pathway, until into the third container, the intracranial container. The connection between the first two containers is called the laminar cribrosa, which is the part of the sclera that is pierced by the axons. The intracranial container is a bone structure without space to expand. So, the intracranial pressure change can severely impair the neuro structures. At the same time, the ocular container is constructed with compact fiber tissues, also with no space to expand.