Background Posterior capsule opacification(PCO) is the main cause inducing low vision after extacapsular cataract extraction. Our previous study determined that polylysine-ethylene diamine tetraacetic acid (EDTA) (PLE) can suppress the incidence of PCO. Objective The goal of this experiment was to investigate the inhibition of polylysine-EDTA on rabbit lens epithelial cells (LECs) proliferation in vitro and the effective concentrations of polylysine-EDTA. Methods The anterior capsular membranes from 10 3-month-old clean New Zealand white rabbits were digested and then cultured to obtain the LECs. The second and third generation of LECs were inoculated on the 96-hole culture plate with the cell density of the 1 × 105/ml. 12.5,25.0,50. 0,100. 0 μmol/Lof PLE were added into the culture medium for 48 hours respectively,and the DMSO medium was used at the same way as the control group. The proliferation of the LECs was then detected by MTT method and the inhibitory rate of PLE on LECs growth was calculated. Results LECs grew at a near normal state in ≤25.0 μmol/L PLE groups,however,cultured LECs were out of shape and the numbers decreased with the weakened adhesion ability in ≥50.0 μ mol/L PLE groups. The A490 values of LECs were 0. 278±0. 013,0. 266±0. 028,0. 260±0. 022 and 0. 247±0. 012 in 12. 5,25.0,50. 0, 100. 0 μmol/L polylysine-EDTA groups respectively and were lower than 0. 311 ±0. 038 of DMSO control group( P=0. 035,0. 011,0. 009,0.013 ). The inhibitory rates of 12. 5,25.0,50. 0, 100.0 μmoL/L PLE on LECs proliferation were 10.61％ , 14.47％ , 16.40％ and 20. 58％ respectively. Conclusions Polylysine-EDTA can inhibit the growth and proliferation of LECs in vitro at a dose-dependent manner.

Objective Posterior capsular opacification (PCO) is one of the most common complications after cataract surgery.The major cause of PCO is the proliferation of residual lens epithelial cells (LECs)after surgery.This study was to determine the feasibility of using a polylysine-EDTA (PLE) conjugate to lens capsule during surgery to remove LECs and its prevention for PCO. Methods EDTA was conjugated to polylysine using (1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride following the instructions of Pierce.Extracapsular cataract extraction (ECCE) was performed on 18 eyes of 9 Japanese white rabbits,and 5 mL of 20 mmol/L EDTA or PLE containing 10 mmol/L EDTA was injected into the capsular bag separately in 6 eyes for 2 minutes and removed using compound sodium chloride injection,and the equal amount compound sodium chloride injection was used in 6 control eyes.Ocular anterior segment inflammation,including cornea,anterior chamber and posterior capsule in postoperation was assessed under the slitlamp microscope.The rabbits were sacrificed and the eyes were enucleated in the 28th day and the section of eye tissue was prepared for histological examination under the light microscope. Results Some inflammation was seen in the all 18 eyes in 3 days following surgery and diminished 2 weeks later following the use of antimicrobial.Obvious cellular proliferation was seen in the anterior capsular membrane,showing the spindle line of cells over 10 layers at equator of lens in the control group.A severer cellular proliferation was seen in the EDTA group.However,Only a few residual cells were found and there was not obvious proliferation on the anterior capsular membrane in PLE group. Conclusion PLE presents an ideal result in removing the residual LECs and decreasing the proliferation in comparison with EDTA.

Objective: To study the influence of fluoride on the endoplasmic reticulum of cultured chondrocytes and antagonizing effect of superoxide dismutase (SOD). Methods: The chondrocytes from the Wistar rat were cultured in vitro for 7 days. Various concentrations of fluoride with or without SOD were added to the cultured medium. Endoplasmic reticulum was observed using laser scanning confocal microscope (LSCM). Results: The average optical density (AOD) of endoplasmic reticulum was obviously decreased respectively cultured with 40, 80 and 160 μmol/L of NaF, while it was obviously increased when 80 μmol/L and 160 μmoL/L of NaF was added with SOD. Conclusions: (1) Endoplasmic reticulum is influenced by various concentration of fluoride. (2) SOD has antagonistic effect on the damage caused by fluoride, especially by high level of fluoride. (3) Quantitative analysis on the endoplasmic reticulum of chondrocytes with laser scanning confocal microscope accurately identifies the functioning state of chondrocytes under fluorosis.