Microplastics (MPs) have been increasingly detected in human tissues. However, their presence in the female reproductive system remains largely unexplored. In this study, micro-Raman spectroscopy was employed to analyze 60 human uterine tissue samples, including those from patients with uterine fibroids, endometrial cancer, cervical cancer, and healthy controls. MPs were detected in 91.1 % of diseased uterine tissue samples, with a mean abundance of 3.0 +/- 1.53 particles per gram after blank correction. A total of fifteen polymer types were identified, predominantly polyethylene (18.0 %), polypropylene (12.9 %), and polystyrene (8.6 %). Most particles measured less than 30 mu m in length and width. High similarity was observed in MP particle abundance, polymer composition, and size distribution across different types of diseased uterine tissues (p < 0.01). Notably, the mean MP abundance in diseased tissues exceeded that in healthy uterine tissues (1.53 +/- 1.09 particles per gram). Furthermore, MP abundance in diseased samples showed associations with patient age and body mass index. These findings provide evidence of widespread MP accumulation in human uterine tissues and reveal distinct differences between diseased and healthy states. This study offers novel insights into environmental MP exposure within the female reproductive system.

Cancer-associated fibroblasts (CAFs) are central architects of immunosuppression and therapy resistance across malignancies, yet how tumor-intrinsic genomic instability instructs stromal reprogramming remains unresolved. Integrated single-cell transcriptomics and epigenomics of samples from patients with high-grade serous ovarian carcinoma revealed POSTN+ myofibroblast-like cancer-associated fibroblasts (myCAFs) and effector regulatory T cells (eTreg cells) as critical mediators of immunosuppression in tumors with high genomic instability. Mechanistically, unstable genomes activated tumor-intrinsic STING signaling, triggering WNT3a/7a secretion. WNT/β-catenin signaling in fibroblasts established a POSTN-dependent positive feedback loop that epigenetically locked cells into a POSTN+ myCAF lineage. These myCAFs reciprocally expanded eTreg cells and exhausted CD8+ T cells, thereby converting genomic instability-driven immune activation into suppression and limiting poly(ADP-ribose) polymerase inhibitor (PARPi) efficacy. Therapeutic POSTN blockade reinvigorated T cell cytotoxicity, depleted eTreg cells, and potentiated PARP inhibition in ovarian and breast cancer models, overcoming resistance. Our work resolves the dual roles of genomic instability and identifies POSTN as a stromal-specific checkpoint to mediate immunosuppression in genomically unstable tumors.

BACKGROUND:Various obesity metrics are used to assess female infertility risk, but their relative prognostic value remains unclear. This cross-sectional study systematically evaluated 16 anthropometric indices to identify the most effective predictors. METHODS:Using National Health and Nutrition Examination Survey (NHANES) 2017-2020 data (n = 1154 reproductive-aged women), we applied a three-phase framework: (1) weighted restricted cubic spline (RCS) models were applied to examine potential non-linear associations between 16 anthropometric obesity indicators and female infertility risk, with univariate analyses used to assess their statistical associations, (2) two model states ('real-world settings' and 'ideal-world settings') were constructed based on the union and intersection of indicator datasets, and weighted logistic regression models were developed for each state and (3) predictive performance and clinical utility were assessed using receiver operating characteristic (ROC) curves, calibration curves and decision curve analysis (DCA), with key diagnostic metrics compared to identify optimal predictors. RESULTS:Thirteen obesity indicators showed predictive value for infertility; Anthropometric Body Shape Index (ABSI) exhibited a U-shaped association, whereas the others demonstrated positive linear relationships. Body mass index (BMI), Weight-Adjusted Waist Index (WWI) and Conicity Index (CI) demonstrated relatively better predictive performance (AUC > 0.65), with WWI showing slightly stronger association with infertility than the other indicators in both model states (adjusted OR = 1.40, 95% CI: 1.10-1.77; AUC = 0.656; results were consistent across the two model states). CONCLUSIONS:Thirteen obesity indicators predicted infertility; ABSI was U-shaped, others were linear. WWI was slightly superior, though differences were modest.

This study aims to uncover the role and mechanism of transforming growth factor α (TGFA) on the malignant progression of cervical cancer. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting were used to examine the expression levels of TGFA in cancer cells and tissues. Changes in cell viability, apoptosis, and malignant metastatic ability of cancer cells were detected using methylthiazolyldiphenyl-tetrazolium bromide (MTT), flow cytometry, and Transwell method respectively. Autophagy was evaluated via microtubule-associated proteins light chain 3 (LC3) and sequestosome 1 (p62) expression. A nude mouse xenograft model was used for in vivo validation. RNA pull-down assay was performed to explore the interaction between TGFA and desmoglein 2 (DSG2). These results indicated that TGFA expression was elevated in both cervical cancer tissues and cells. TGFA overexpression promoted cell proliferation, metastasis, and autophagy, whereas TGFA knockdown exerted the opposite effects and inhibited tumor growth. Mechanistically, TGFA bound to DSG2 and affected the downstream MYC oncogene (c-MYC)/ADAM metallopeptidase domain 17 (ADAM17) pathway. In conclusion, TGFA serves as an upstream regulator of the DSG2/c-MYC/ADAM17 axis, which is correlated with autophagy and malignant progression of cervical cancer.

Purpose:The menopausal transition is accompanied by metabolic changes in women. This study explores the Chinese visceral adiposity index (CVAI) as a potential indicator of menopausal status to aid in disease prevention. Patients and Methods:A cohort of 404 premenopausal Chinese women aged 45 years and above, from the China Health and Retirement Longitudinal Study (CHARLS), was included. CVAI was calculated from the 2011 survey data, menopausal status was collected in the 2018 survey, representing a 7-year longitudinal follow-up. The cross-sectional study cohort from the National Health and Nutrition Examination Survey (NHANES) included 3577 women aged 40-60 with CVAI and self-reported menopausal status from 2003 to 2020. Logistic regression models were estimated the odds ratio (OR) of the menopause data and 95% confidence intervals (CIs). Results:In the CHARLS cohort, the adjusted OR for menopause in the fourth quartile of CVAI compared to the first quartile was 5.23 (95% CI: 1.59, 17.17; P for trend: 0.005). Additionally, a significant difference in the association between menopausal status and CVAI was found between rural and urban populations (P for interaction = 0.029). Moreover, in the NHANES cohort, the CVAI and the menopausal status were associated after adjustment (OR: 1.02, 95% CI: 1.002, 1.037, P: 0.029). In the stratified analysis, the association of CVAI with the status of menopause was observed among other ethnicities which including Asians (OR: 1.092, 95% CI: 1.012, 1.178, P: 0.025). Finally, a nomogram was developed to facilitate the clinical assessment of menopause based on the CVAI. Conclusion:The CVAI demonstrated a significant association with the odds of menopausal status in both Chinese and the US populations, suggesting its potential as a correlative marker for menopausal status, but the associational strength may vary by population.

Poly(ADP-ribose) polymerase inhibitors (PARPi) have revolutionized the treatment of homologous recombination-deficient (HRD) tumors, yet their efficacy in homologous recombination-proficient (HRP) tumors is still limited. Here, we pinpoint lysyl oxidase-like 2 (LOXL2) as a key epigenetic regulator driving PARPi resistance. Our study demonstrate that elevated LOXL2 expression correlates with poor prognosis and disease recurrence in high-grade serous ovarian cancer (HGSOC) patients. Functional studies reveal that LOXL2 depletion or pharmacological inhibition synergizes with PARPi to suppress HRP models of both ovarian and breast cancer. Mechanistically, LOXL2 directly interacts with and transcriptionally activates BRD4, a core component of the super-enhancer complex, thereby amplifying the expression of DNA damage repair (DDR) genes such as MDC1, KAT5, and USP7. Strikingly, LOXL2 inhibition induces a "BRCAness" phenotype in HRP tumors, rendering them more susceptible to PARPi by impairing DDR capacity. Combining BET inhibitors with PARPi abrogates LOXL2-mediated resistance, underscoring BRD4 dependency in this process. Our findings establish LOXL2 as a druggable epigenetic target to overcome PARPi resistance in HRP models of multiple tumor types, presenting a therapeutic strategy independent of HR status and holding significant clinical potential for expanding PARPi benefits to a broader patient population.

With increasing global awareness of plastic pollution, microplastics (MPs) have emerged as a potential environmental health hazard. While MPs have been detected in various human tissues, their relevance to gynecological malignancies, particularly endometrial cancer (EC), remains largely unexplored. This study aims to evaluate MP accumulation in EC tissues and investigate its potential impact on tissue metabolic reprogramming. Raman spectroscopy, combined with untargeted metabolomics, was employed to comprehensively assess both the presence and metabolic consequences of MPs in EC and matched normal endometrial tissues. In 32 analyzed samples, MPs averaged 3.2 ± 2.3 particles/g, with polyethylene (15.7 %), polypropylene (11.8 %), ethylene-acrylic acid (10.8 %), and polystyrene (8.8 %) predominating. Endometrial cancer tissues exhibited significantly higher MP levels (3.7 ± 2.5 particles/g) than normal controls (2.0 ± 1.5 particles/g). Metabolomic profiling revealed that MP exposure is associated with substantial alterations in cancer-related metabolic pathways, with the glycine, serine, and threonine metabolism pathway showing the most pronounced enrichment. Key differential metabolites included glycine, N-acetyl-arginine, and 4-aminobutyric acid, which have been implicated in tumor proliferation and immune regulation. These findings suggest that MPs may promote the development of EC through modulation of metabolic pathways, providing novel insights into the role of MPs in gynecological cancers. This study highlights the emerging threat of environmental pollution to women's reproductive health and offers innovative theoretical perspectives for future research.

Background:Unhealthy lifestyle habits, such as smoking, can impact oxidative stress. During oxidative stress, unnaturalized free radicals can damage DNA, proteins, and lipids, leading to cellular damage and death. A comprehensive measurement of various pro-oxidative and antioxidative exposures can reflect an individual's oxidative stress burden. However, studies on assessing the association between dietary and lifestyle factors related to oxidative stress and menopause were previously lacking. Materials and methods:A cohort of 2,813 women aged 40-60 years from the National Health and Nutrition Examination Survey conducted between 2003 and 2020 was identified as meeting the eligibility criteria. The associations of oxidative balance score (OBS) with the menopausal status were examined via weighted logistic regression models, and the odds ratios (ORs) of menopause onset were calculated with 95% confidence intervals (CIs). Machine learning models were developed and compared to classify the menopausal status based on the OBS and other epidemiological factors, with the interpretability of the models explored using the Shapley Additive Explanations method. Results:Following adjustment for various confounding factors, OBS was reversely associated with menopause (OR: 0.97, 95% CI: 0.94-0.99, p = 0.010). When the OBS was categorized into quartiles, the association with menopause was still significant (p for trend = 0.009). The association of the OBS with menopause remained significant after excluding any each survey year cycles (p for trend < 0.050). The menopause classification models developed using TabFPN, Random Forest, CatBoost, and XGBoost achieved an area under the curve of 0.880, 0.884, 0.886, and 0.878, respectively. Based on the results from epidemiological analysis and machine learning models, the intake of magnesium, zinc, niacin, and vitamin B6 showed a decline in the early postmenopausal period and contributed in the model performance. Conclusions:OBS were reversely associated with the menopausal status, and the OBS might serve as an indicator of an individual's oxidative stress status for lifestyle interventions during the menopausal transition.

Microplastics (MPs) are synthetic solid particles or polymer matrices that range in size from 1 μm to 5 mm. MPs are widely present in the global biosphere, leading to increasing concerns about their impact on human health. In this study, micro-Raman spectroscopy was used to evaluate the presence and characteristic of MPs in adenomyosis, ovarian ectopic cysts, and uterine tube tissue samples from 60 females. MPs were detected in all human samples at an average level of 1.5 ± 1.2 particles per g of tissue (Average 1.40 ± 1.11 particles per g of tissue after blank correction.). Among these MPs, a total of 11 polymer types were identified. MPs are mainly composed of polyethylene (PE, 31 %), polypropylene (PP, 22 %) and PE-co-PP (11 %). These MPs had an average length of 15.15 ± 6.45 μm and an average width of 12.56 ± 6.65 μm, with the majority (70 %) measuring less than 20 μm in size. Most MPs were fibers (38.9 %) and fragments (24.4 %). A significant correlation (p <0.05) was found between the sizes of MPs detected across the three disease samples, with PE and PP being the most frequently identified types. This study demonstrates the presence of MPs in diseased tissues from patients with adenomyosis, ovarian ectopic cysts, and uterine tubes, providing evidence for the presence of MPs in reproductive system tissues.

To develop predictive nomograms of lymph vascular space invasion (LVSI) in patients with early-stage cervical cancer. We identified 403 patients with cervical cancer from the Affiliated Hospital of Jiangnan University from January 2015 to December 2019. Patients were divided into the training set (n = 242) and the validation set (n = 161), with patients in the training set subdivided into LVSI (+) and LVSI (−) groups according to postoperative pathology. Preoperative hematologic indexes were compared between the two subgroups. Univariate and multivariate logistic regression analyses were used to analyze the independent risk factors for LVSI, from which a nomogram was constructed using the R package. LVSI (+) was present in 94 out of 242 patients in the training set, accompanied by a significant increase in the preoperative squamous cell carcinoma antigen (SCC), white blood cells (WBC), neutrophil (NE), platelet (PLT), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), systemic inflammation index (SII), and tumor size (P < 0.05). Univariate analysis showed that SCC, WBC, NE, NLR, PLR, SII, and tumor size were correlated with LVSI (P < 0.05), and multivariate analysis showed that tumor size, SCC, WBC, and NLR were independent risk factors for LVSI (P < 0.05). A nomogram was correspondingly established with good performance in predicting LVSI [training: ROC-AUC = 0.845 (95

Microplastics (MP) are plastic particles smaller than 5 millimeters. MPs are widely present in the global biosphere, leading to increasing concerns about their impact on human health. In this study, micro-Raman spectroscopy was used to evaluate the presence and characteristic of MPs in tissue samples from 18 female with adenomyosis, ovarian ectopic cysts, and fallopian tubes. MPs were detected in all human samples at an average level of 1.39 ± 1.05 particles/g (Average 1.22 ± 0.97 particles/g of tissue after blank correction.). Among these MPs, a total of seven polymer types were identified. MPs are mainly composed of polyethylene (PE, 32%), polypropylene (PP, 24%) and PE-co-PP (20%). The size of these MPs ranged from 4.51 to 32.93 μm, and most of them (96%) were smaller than 20 μm. Most MPs is in the form of fibers (48%) and films (24%). It was found that there were differences in the concentration, type and size of MPs detected in the three disease samples. This study provides support for understanding the shape, size and type of MPs in human adenomyosis, ovarian ectopic cysts and fallopian tubes.

Abstract The authors have requested that this preprint be removed from Research Square.

目的:探讨不同盆腔淋巴结切除方式联合盆腔内注射铜绿假单胞注射液对宫颈癌术后盆腔淋巴囊肿及下肢淋巴水肿形成的影响.方法:收集2015年1月至2020年12月江南大学附属医院妇科收治的Ⅰb～Ⅱa期(术前)宫颈癌患者,均行经腹根治性全子宫切除+盆腔淋巴结切除术.将患者分为四组:观察组1(保留髂外末端淋巴结)、对照组1(系统盆腔淋巴清扫术)、观察组2[铜绿假单胞菌注射液(PA-MSHA)+保留髂外末端淋巴结]、对照组2(PA-MSHA+系统盆腔淋巴清扫术).分别比较四组术后1～7天引流量,引流时间,术后7天拔管率;术后4周、8周行超声或CT检查,评价盆腔淋巴囊肿及下肢淋巴水肿形成情况.结果:除术后第一天外,各观察组术后第2～7天盆腔引流量明显减少,引流时间明显缩短,术后7天拔管率均高于对照组,术后盆腔淋巴囊肿及下肢淋巴水肿形成率观察组均低于对照组,差异均有统计学意义(P＜0.05).观察组2术后引流时间较观察组1缩短,术后7天拔管率高于观察组1,差异有统计学意义(P＜0.001).观察组2与观察组1比较,盆腔淋巴囊肿形成率及下肢水肿发生率有降低趋势,但差异无统计学意义(P＞0.05).结论:保留髂外末端淋巴结联合盆腔内注射PA-MSHA,可明显减少早期宫颈癌患者术后盆腔引流量,并缩短置管时间,还可减少盆腔淋巴囊肿及下肢淋巴水肿发生率.

Background Of gynecologic malignancies, ovarian cancer is the leading cause of death, mainly due to the lack of sensitive tumor markers, which means it almost always presents at an advanced stage. Exosome Component 4 (EXOSC4) is involved in RNA degradation, but its role in epithelial ovarian cancer (EOC) is unclear. Methods The expression levels of EXOSC4 in EOC and normal ovarian tissue specimens were determined by immunohistochemical staining. The overall survival (OS) and progression-free survival (PFS) of patients with EOC were evaluated after patients were classified into high and low EXOSC4 expression groups, and the Cox regression model was established to identify independent predictors of patient prognosis. The effects of EXOSC4 on proliferation, colony formation, migration, and invasion were examined in the SKOV-3 and HO8910 cell lines by lentivirus-mediated shRNA knockdown. Flow cytometry was used to detect cell cycle changes. The mRNA levels of cyclin D1, CDK4, and c-myc were detected by RT-PCR. The protein expression levels of β-catenin, cyclin D1, CDK4, c-myc, vimentin, N-cadherin, and E-cadherin were assessed by western blot. Wnt/β-catenin activation was measured by TCF/LEF reporter assay. Results EXOSC4 was significantly elevated in EOC tissues and cell lines. High EXOSC4 expression was correlated with the International Federation of Gynecology and Obstetrics (FIGO) stage and pathological grade, and identified as an independent predictor of shorter OS and PFS. EXOSC4 knockdown suppressed proliferation, migration, and invasion in EOC cell lines. Cells were arrested at G0/G1 phase after EXOSC4 knockdown. The mRNA levels of cyclin D1, CDK4, and c-myc were decreased. β-catenin, cyclin D1, CDK4, c-myc, vimentin, and N-cadherin protein expression levels were reduced, while those of E-cadherin was increased. Wnt/β-catenin activity was suppressed after the EXOSC4 knockdown. Conclusions EXOSC4 is involved in EOC. Knockdown of EXOSC4 can inhibit the proliferation, migration, and invasion ability of EOC by suppressing the Wnt pathway. EXOSC4 is expected to be a novel biomarker and molecular target in EOC.

Background: Cesarean scar pregnancy (CSP) involves a rare form of placental attachment that often leads to life-threatening conditions. The best treatment for CSP has been debated for decades. We aimed to evaluate the different treatments for CSP and analyzed the risk factors for intraoperative hemorrhage. Methods: CSP patients treated at the Affiliated Hospital of Jiangnan University were reviewed retrospectively from January 2014 to 2020. CSP was classified into three types based on the location and shape of gestational tissue, blood flow features, and thickness of the myometrium at the incision site. The clinical characteristics, types, approaches of treatment, and clinical outcomes of CSP were analyzed. Results: A total of 55 patients were included in this study, 29 (52.7%) of whom underwent transvaginal curettage after uterine artery embolization (UAE) and 22 (40%) of whom underwent transabdominal ultrasound-guided hysteroscopic curettage (USHC) in type I and II. Four patients (7.3%) classified as type III underwent laparoscopic cesarean scar resection (LCSR). Intraoperative blood loss, blood transfusion rate, and scar diverticulum were significantly higher in type II than in type I (P < 0.05). Even though USHC showed no differences in intraoperative blood loss, length of stay, and scar diverticulum compared with curettage after UAE (P > 0.05), superiority was found in surgical time and hospitalization cost (P < 0.05). Furthermore, the type of CSP (OR = 10.53, 95% CI: 1.69–65.57; P = 0.012) and diameter of the gestational sac (OR = 25.76, 95% CI: 2.67–248.20; P = 0.005) were found to be risk factors for intraoperative hemorrhage. Conclusions: Transabdominal ultrasound-guided hysteroscopic curettage is an effective and relatively safe treatment option for patients with CSP. Type of CSP and diameter of the gestational sac were found to be associated with excessive intraoperative hemorrhage.

目的:探讨EXOSC4在调节宫颈癌细胞增殖、迁移和侵袭方面的作用及机制.方法:通过GEPIA在线数据库分析EXOSC4在宫颈癌中的表达及预后情况,Western blot法检测人宫颈癌细胞株中EXOSC4的表达,设计合成靶向EXOSC4的特异性shRNA,构建稳定低表达EXOSC4的宫颈癌细胞株.Western blot和荧光定量PCR验证干扰效率;CCK-8和细胞克隆形成实验检测各组HeLa细胞的增殖能力;采用流式细胞仪检测细细胞周期;采用细胞划痕和Transwell实验检测HeLa细胞的迁移和侵袭能力,Western blot法检测EMT相关蛋白(E-cadherin、N-cadherin、Vimentin)和Wnt/β-catenin通路相关蛋白(β-catenin、c-Myc、cyclin D1)的表达水平.结果:宫颈癌组织中EXOSC4表达明显高于正常组织,EXOSC4高表达提示预后不良.与HCC94和SiHa细胞系相比,EXOSC4在HeLa细胞中表达量最高.抑制EXOSC4表达可显著降低宫颈癌细胞的增殖、迁移和侵袭能力,细胞周期阻滞在G0/G1期,上调E-cadherin表达,下调N-cadherin、Vimentin、β-catenin、c-myc和Cyclin-D1表达(均P<0.05).结论:沉默EXOSC4可通过Wnt/β-catenin信号通路抑制HeLa细胞的增殖、迁移和侵袭.

目的:分析上皮性卵巢癌肝转移的独立预后因素,构建并验证预测患者癌症特异性生存率(CSS)的列线图.方法:回顾性分析SEER数据库中2010年至2013年确诊的329例卵巢癌肝转移患者的临床资料,应用Kaplan-Meier法绘制生存曲线,Log-rank检验评价各因素不同亚组生存期的差别,通过COX多因素回归分析获得独立预后因素,运用R软件绘制列线图.列线图的预测性能由校准图和ROC曲线下面积(AUC)进行内部验证.一致性指数(C-index)评估模型的精确性.结果:年龄≥73岁、黏液性癌、透明细胞癌和癌肉瘤是CSS的独立危险因素;T3期、仅细胞减灭术、细胞减灭术联合Ⅱ期肝切除术和化疗是CSS的独立保护因素.列线图内部验证C-index为0.692(95％CI:0.651～0.733).所建列线图预测确诊后1、3年CSS的预测区分度分别为0.802和0.723.校准曲线均呈现出良好的一致性.结论:基于SEER数据库确立了上皮性卵巢癌肝转移患者的独立预后因素,列线图预测效果良好,具有快速准确评估患者生存预后的应用价值.

Background Ovarian cancer typically is diagnosed late because insensitivity and lack of specificity of current biomarkers prior to its clinical detection. Ribosomal protein S6 (RPS6) is a ribosomal protein involved in the ribosomal 40S subunit, but its biological role in epithelial ovarian cancer (EOC) is still unknown. Results RPS6 was elevated in EOC compared to normal ovarian tissues and adenomas. Higher expression of RPS6 predicted worse prognosis in EOC. The level of RPS6 was correlated with clinical stage, histological type and pathological grade. Knockdown of RPS6 reduced the proliferation of ovarian cancer cell lines SKOV-3 and HO8910, and inhibit the migration and invasion ability. It revealed that cells arrested at G0G1 phase after knockdown of RPS6, and the expressions of CyclinD1, Cyclin E, CDK2, CDK4, CDK6 and pRb were also reduced. Conclusions RPS6 is involved in EOC and knockdown of RPS6 could inhibit the proliferation, invasion and migration ability of EOC in vitro by inducing G0/G1 phase arrest. RPS6 is expected to be a novel biomarker and molecular target to the EOC.

目的 探讨宫颈长度测量联合胎儿纤维连接蛋白(fFN)测定对早产的预测价值.方法 采用ELISA定量测定28～34周先兆早产孕妇阴道分泌物中的fFN水平,同时经阴道B超测量宫颈长度.分析fFN定量及测量宫颈长度对7d内早产的预测价值.结果 353例有先兆早产症状的孕妇中,34例(9.6％)在7 d内发生早产.在宫颈长度＜20 mm的孕妇中,当取25 ng/ml作为fFN临界值时,31例被认为是低风险,其中1例(3.2％)在7d内发生早产.fFN≥25 ng/ml者的7d早产率高于＜25 ng/ml者(29.3％vs.3.2％)(P＜0.01).在宫颈长度≥20 mm的孕妇中,当fFN临界值取200 ng/ml时,63例被认为是高风险,其中6例(9.5％)在7d内发生早产.fFN≥200 ng/ml者的7d早产率高于＜200 ng/ml者(9.5％vs.2.7％)(P＜0.05).有先兆早产症状的孕妇7d早产率与宫颈长度呈负相关(P＜0.05),而与fFN浓度呈正相关(P＜0.05).结论 fFN定量检测联合宫颈长度测量能够提高先兆早产孕妇在7d内早产的预测价值.