Objective To explore the relationship between the expression level and localization of CD80 and carcinoembryonic antigen(CEA) in the human fetal thymus and its development. Methods Twelve aborted dead fetus samples(via donation from the dead fetus's parents and permission of the hospital agency) were collected from Department of Genecology and Obstetrics in the affiliated hospital,including 4 cases aged less than 4 months in earlier stage and 8 cases aged 5 months in later stage. The two groups of earlier and later aged samples were randomly divided into two aliquots. One aliquot was prepared for frozen sections stained with HE,ANAE histochemistry,CD80 and carcinoembryonic antigen (CEA) immunohistochemistry; and detected with CEA ELISA. The protein extracted from another aliquot with Trizol reagent was detected with CD80 immunoblotting and CEA ELISA. Results The thymus in earlier aged group,especially the medulla was less developed. The reticulo-epithelial cells (RECs) with CD80-IR in the later aged group could be classified into six patterns. The type I RE in flatten shape were located at the inner surface of the capscule and trabecula. The type II RECs (DCs) in small satellite shaped were localized in the medulla. Both type III and type IV RECs were located at the dzemarcation between the cortex and medulla. The type V RECs (DCs) in large satellite shaped with delicate processes to connect into reticular meshes were distributed in the medulla and the type VI RECs were found in the Hassall's corpuscles. The ANAE-dot pattern T cells (CD4+) and ANAE-diffuse granular pattern T cells (CD8+) in separate clusters could be found in the reticular pores. In the immunoblotting and ELISA the expression level of both CD80-IR and CEA-IR in the later aged group was higher than that in the earlier aged group.Conclusion The results suggest that the expression and cocalization of CD80 and CEA closely related to the development and function of fetal thymus.

Objective: To explore effect of quercetin on antiapoptosis damage in NIH-3T3 cells.Methods: The NIH-3T3 cells in logarithmic growth phase incubated in DMEM were divided into 4 groups as follows.① Q pre-protection group(Qb);prior to incubation with 0.5 mmol/LH2O2 for 30 min,the NIH-3T3 cells were incubated with 50umol/L quercetin for 24 h.② Q post-protection group(Qa): after incubation with 0.5 mmol/L H2O2 for 30 min,the NIH-3T3 cells were incubated with 50umol/L quercetin for 24 h.③ H2O2 damage group(H2): the NIH-3T3 cells were incubated with 0.5 mmol/L H2O2 for 30 min followed by subsequent incubation with 10% FBS for 24h.④ The control group(C): the NIH-3T3 cells were incubated with DMEM medium supplemented with 10% FBS.The apoptosis was detected by DNA ladder and TUNEL.The expressions of Bcl-2,Bax and Caspase-3 were demonstrated with immunocytochemistry.The NIH-3T3 cell survival rate was detected by MTT assay.Results: The apoptosis in the Qb group was decreased than that in the Qa and H2 groups.The Qb group compared with Qa and H2 groups,the expressions of Bcl-2 was increased;Bax and Caspase-3 were attenuated;and the NIH-3T3 cell survival rate was promoted.Conclusion: The anti-apoptosis effect of quercetin damage in the NIH-3T3 cells may act through down-regulated expressions of Bax and Caspase-3 and up-regulated expression of Bcl-2.

Objective To explore the protection effect of quercitin(Q)on oxidative damage in NIH-3T3 cells.Methods The NIH-3T3 cells in logarithmic growth phase incubated in DMEM were divided into 4 groups as follows. (1)Q pre-protection group (Q1) prior to incubation with 0.5mmol/L H2O2 for 30min. the 3T3 cells were incubated with 50μmol/L quercetin for 24h(2)Q post-protection group (Q2) after incubation with 0.5mmol/L H2O2 for 30min. the 3T3 cells were incubated with 50μmol/L quercetin for 24h(3)H2O2 damage group(H2) the 3T3 cells were incubated with 0.5mmol/L H2O2 for 30min followed by subsequent incubation with 10% fetal bovine serum(FBS) for 24h(4)The control group(C) the 3T3 cells were incubated with DMEM medium supplemented with 10% FBS. The T-AOC. SOD. GSH-Px. GSH. NOS. NO and MDA were detected in the lysate of harvested each group cells. The 3T3 cell survival rate was detected by MTT assay.Results The levels of T-AOC.SOD.GSH-Px.GSH were enhancedMDA was decreased and NO.NOS had insignificant changeThe 3T3 cell survival rate was promoted markedly.Conclusion The enhanced anti-oxidative effect of quercetin on the 3T3 cells may act through up-regulation of a series of anti-oxidative enzymen system.